Restriction fragment length polymorphism of 195 bp repeated satellite DNA of Trypanosoma cruzi supports the existence of two phylogenetic groups.

Abstract:

:The restriction fragment length polymorphism of the 195 bp repeated DNA sequence of Trypanosoma cruzi was analyzed among 23 T. cruzi stocks giving a reliable picture of the whole phylogenetic variability of the species. The profiles observed with the enzymes Hinf I and Hae III were linked together and supported the existence of two groups. Group 1 shows a 195 bp repeated unit (Hinf I) and high molecular weight DNA (Hae III), while group 2 presents a ladder profile for each enzyme, which is a characteristic of tandemly repeated DNA. The two groups, respectively, clustered stocks pertaining to the two principal lineages evidenced by isoenzyme and RAPD markers. The congruence among these three independent genomic markers corroborates the existence of two real phylogenetic lineages in T. cruzi. The specific monomorphic profiles for each major phylogenetic lineage suggest the existence of ancient sexuality and cryptic biological speciation.

journal_name

Mem Inst Oswaldo Cruz

authors

Bastrenta B,Bosseno MF,Barnabé C,Tibayrenc M,Brenière SF

doi

10.1590/s0074-02761999000300008

subject

Has Abstract

pub_date

1999-05-01 00:00:00

pages

323-8

issue

3

eissn

0074-0276

issn

1678-8060

pii

S0074-02761999000300008

journal_volume

94

pub_type

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