Development of a real time polymerase chain reaction for quantitation of Schistosoma mansoni DNA.

abstract：:Studies on the sandfly fauna to evaluate natural infection indexes are still limited in the Brazilian Amazon, a region with an increasing incidence of cutaneous leishmaniasis. Here, by using a multiplex polymerase chain reaction directed to Leishmania kDNA and hybridisation, we were able to identify L. (Viannia) subge...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Araujo-Pereira T,Pita-Pereira D,Boité MC,Melo M,Costa-Rego TA,Fuzari AA,Brazil RP,Britto C

更新日期：2017-01-01 00:00:00

abstract：:In previous studies it was shown that the recombinant molecule, r-Sm14, induces high levels of protection against Schistosoma mansoni infection in two outbred animal models and immune crossprotection against infection by Fasciola hepatica in Swiss outbred mice. r-Sm14 was derived from a living worm extract, called SE,...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Thaumaturgo N,Vilar MM,Diogo CM,Edelenyi R,Tendler M

更新日期：2001-01-01 00:00:00

abstract：:A sylvatic Triatoma infestans DM (dark morph) population detected in the Bolivian Chaco was characterized and compared with various domestic ones. The degree of differentiation of DM was clearly within the T. infestans intra-specific level. Nevertheless marked chromatic and morphometric differences as well as differen...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Noireau F,Bastrenta B,Catalá S,Dujardin JP,Panzera F,Torres M,Perez R,Galvão C,Jurberg J

更新日期：2000-01-01 00:00:00

abstract：:Two of the major problems facing the Amazon - human migration from the other areas and uncontrolled deforestation - constitute the greatest risk for the establishment of endemic Chagas disease in this part of Brazil. At least 18 species of triatomines had been found in the Brazilian Amazon, 10 of them infected with Tr...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Coura JR,Junqueira AC,Boia MN,Fernandes O

更新日期：1999-01-01 00:00:00

abstract：:Domiciliated Rhodnius prolixus and sylvatic R. colombiensis were analyzed in order to confirm their genetic divergence and verify the risk that the latter represents in the domiciliation process, and to provide tools for identifying the sources of possible reinfestation by triatomines in human dwellings allowing contr...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Jaramillo C,Montaña MF,Castro LR,Vallejo GA,Guhl F

更新日期：2001-11-01 00:00:00

abstract：:Methicillin resistant Staphylococcus aureus (MRSA) is an organism that is frequently transmitted in hospitals and perinatal units. The MRSA is considered a public health problem in neonatology because of its strong potential for dissemination in the wards associated with high rates of morbidity and mortality. In this ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Loureiro MM,de Moraes BA,Quadra MR,Pinheiro GS,Suffys PN,Asensi MD

更新日期：2000-11-01 00:00:00

abstract：:We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputu...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Cardoso CL,Giacomelli LR,Helbel C,Sant'Ana JJ,Martins FM,Barreto AM

更新日期：2001-02-01 00:00:00

abstract：BACKGROUND:Zika virus (ZIKV) was recognised as a zoonotic pathogen in Africa and southeastern Asia. Human infections were infrequently reported until 2007, when the first known epidemic occurred in Micronesia. After 2013, the Asian lineage of ZIKV spread along the Pacific Islands and Americas, causing severe outbreaks ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Freire CCM,Palmisano G,Braconi CT,Cugola FR,Russo FB,Beltrão-Braga PC,Iamarino A,Lima Neto DF,Sall AA,Rosa-Fernandes L,Larsen MR,Zanotto PMA

更新日期：2018-05-10 00:00:00

abstract：:The objectives of this work were to determine the prevalence of Babesia bovis, Babesia bigemina, and Anaplasma marginale detecting antibodies in cattle raised in the semi-arid region of the state of Bahia, Brazil, through indirect enzyme linked immunosorbent assays (ELISA) and to compare the performances of indirect e...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Barros SL,Madruga CR,Araújo FR,Menk CF,de Almeida MA,Melo EP,Kessler RH

更新日期：2005-10-01 00:00:00

abstract：:The anesthetic effect of some water-soluble anesthesic or narcotic drugs currently used in mice was tested in molluscs of the Biomphalaria genus. Sodium thiopental was very toxic to the snails resulting in high rates of mortality in all the treatment schedules tested. Cetamine base, at concentration of 0.25 mg/ml of w...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Martins-Sousa R,Negrão-Corrêa D,Bezerra F,Coelho P

更新日期：2001-04-01 00:00:00

abstract：:We have been interested in identifying genes that play a role in reproduction of the mosquito Aedes aegypti. Our interests are currently focused on the vitellogenin genes which in the mosquito are expressed only in the fat body in response to the insect steroid hormone, 20-hydroxyecdysone. Four of the five vitellogeni...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Hamblin MT,Marx JL,Wolfner MF,Hagedorn HH

更新日期：1987-01-01 00:00:00

abstract：:Some species of Amphibia and Reptilia are listed as new hosts of spargana, from material deposited in the Helminthological Collection of Oswaldo Cruz Institute. It is discussed the difficulties in identifying the larvae (Sparganum) and also the identification of adults of Luheella species from South America. The histo...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Rego AA,Schäffer GV

更新日期：1992-01-01 00:00:00

abstract：:Studies based on shell or reproductive organ morphology and genetic considerations suggest extensive intraspecific variation in Biomphalaria snails. The high variability at the morphological and genetic levels, as well as the small size of some specimens and similarities between species complicate the correct identifi...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Vidigal TH,Dias Neto E,Spatz L,Nunes DN,Pires ER,Simpson AJ,Carvalho OS

更新日期：1998-01-01 00:00:00

abstract：:We present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans) that can be found in the Amazon Region. Nested PCR-based identifications...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Tang TH,López-Vélez R,Lanza M,Shelley AJ,Rubio JM,Luz SL

更新日期：2010-09-01 00:00:00

abstract：:Goats from the Venezuelan northern arid zones were found infested by the following nematodes: Haemonchus contortus, Trichostrongylus axei, T. colubriformis, Cooperia curticei, Oesophagostomum columbianum, Skrjabinema ovis y Trichuris globulosa. The Shannon-Weaver index of diversity values oscillated between 0.045 and ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Morales G,Pino LA,Aldana E,Perdomo L,Molina E

更新日期：1986-04-01 00:00:00

abstract：:The present paper reports acuarioid nematodes recovered from avian hosts. A new species of the genus Schistorophus Railliet, 1916 is proposed based mainly on findings referring to ptilina, spicules and vagina. Ancyracanthopsis coronata (Molin, 1860) Chabaud & Petter, 1959 is referred again in Brazil since its proposit...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Pinto RM,Vicente JJ,Muniz-Pereira LC,Noronha D

更新日期：1999-11-01 00:00:00

abstract：:Chronic Chagas disease diagnosis relies on laboratory tests due to its clinical characteristics. The aim of this research was to review commercial enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) diagnostic test performance. Performance of commercial ELISA or PCR for the diagnosis of chron...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Brasil PE,Castro R,Castro Ld

更新日期：2016-01-01 00:00:00

abstract：BACKGROUND:Enterocytozoon bieneusi are the most common microsporidia associated with different clinical manifestations such as diarrhoea, respiratory tract inflammation and acalculous cholecystitis, especially in immunocompromised patients. Infection usually occurs by ingestion of food and water contaminated with spore...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Yamashiro S,Fiuza VRDS,Teixeira ÂTLS,Branco N,Levy CE,Castro ICVS,Franco RMB

更新日期：2017-06-01 00:00:00

abstract：:In this study, HIV-1 viral blood quantitation determined by Nucleic Acid Sequence Based Amplification (NASBA) was compared with other surrogate disease progression markers (antigen p24, CD4/CD8 cell counts and beta-2 microglobulin) in 540 patients followed up at São Paulo, SP, Brazil. HIV-1 RNA detection was statistic...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Sitnik R,Pinho JR

更新日期：1998-05-01 00:00:00

abstract：:In this work, a murine experimental model of toxocariasis has been developed in BALB/c, C57BL/10 and C3H murine strains orally inoculated with 4,000 Toxocara canis embryonated eggs, in order to investigate the isotype-specific immune responses against excretory-secretory antigens from larvae. T. canis specific IgG+M, ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Cuéllar C,Fenoy S,del Aguila C,Guillén JL

更新日期：2001-05-01 00:00:00

abstract：:Experimental infections by Trypanosoma (Megatrypanum) minasense were performed in primates - Saimiri sciureus and Callithrix penicillata - with the objective of searching for morphological variations of the blood trypomastigotes with respect to hosts and time of infection. We carried out morphological and morphometric...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Ziccardi M,Lourenco-de-Oliveira R

更新日期：1999-09-01 00:00:00

abstract：:There is no clear understanding of the outcome of reinfection in New World cutaneous leishmaniasis, and its role in the relationship to the development of protection or secondary disease. For this reason, reinfection experiments with homologous (Leishmania panamensis-L. panamensis) and heterologous (L. major-L. paname...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Osorio Y,Gonzalez SJ,Gama VL,Travi BL

更新日期：1998-05-01 00:00:00

abstract：:The preferred food items of the slugs Laevicaulis alte and the snails Achatina fulica were used to prepare 'poison baits' by injecting the pesticides 'Rogor' and 'Nuvan' to kill these mollusc pests. The 'poison baits' prepared with Trichosanthes dioica and Lycopersicum esculentum were accepted by 100% individuals of b...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Panigrahi A,Raut SK

更新日期：1993-04-01 00:00:00

abstract：:Patterns of parasite abundance and prevalence are thought to be influenced by several host characteristics such as size, sex, developmental stage, and seasonality. We examined two obligatory ectoparasites of the bat Noctilio leporinus (L.) (Chiroptera, Noctilionidae) to test whether prevalence and abundance of Noctili...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Moura MO,Bordignon MO,Graciolli G

更新日期：2003-09-01 00:00:00

abstract：:The presence of tRNA array, a region with high tRNA gene number and density, has been demonstrated in Mycobacterium genus. However, a recent phylogenomic study revealed the existence of five distinct monophyletic groups (genera) within this genus. Considering this new scenario, and based on in-silico analyses, we have...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Morgado SM,Vicente ACP

更新日期：2019-01-01 00:00:00

abstract：:Tungiasis is a parasitic skin disease widespread in resource-poor urban and rural communities in Brazil. Inhabitants of an urban slum in Northeast Brazil were examined for the presence of tungiasis lesions and followed-up twice a week for a period of three weeks. Each time the number, stages, and topographic localizat...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Feldmeier H,Kehr JD,Poggensee G,Heukelbach J

更新日期：2006-02-01 00:00:00

abstract：:Acoustic signalling has been extensively studied in insect species, which has led to a better understanding of sexual communication, sexual selection and modes of speciation. The significance of acoustic signals for a blood-sucking insect was first reported in the XIX century by Christopher Johnston, studying the hear...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Vigoder Fde M,Ritchie MG,Gibson G,Peixoto AA

更新日期：2013-01-01 00:00:00

abstract：:The antibody response to Plasmodium falciparum parasites of naturally infected population is critical to elucidate the role of polymorphic alleles in malaria. Thus, we evaluated the impact of antigenic diversity of repetitive and family dimorphic domains of the merozoite surface protein 2 (MSP-2) on immune response of...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Sallenave-Sales S,Faria CP,Zalis MG,Daniel-Ribeiro CT,Ferreira-da-Cruz Mde F

更新日期：2007-06-01 00:00:00

abstract：:Many lines of Plasmodium falciparum undergo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and the selected cells produce gametocy...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Kemp DJ,Thompson J,Barnes DA,Triglia T,Karamalis F,Petersen C,Brown GV,Day KP

更新日期：1992-01-01 00:00:00

abstract：:During an epidemiological survey of acute respiratory infection in Rio de Janeiro, among 208 adenovirus isolates, we found two strains that we were not able, by a standard neutralization procedure, to distinguish between type 3 or 7. However, DNA restriction pattern for the two strains with different enzymes were anal...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Moraes MT,da Silva M,Leite JP,Nascimento JP

更新日期：1998-03-01 00:00:00