Cytotoxicity of three new triazolo-pyrimidine derivatives against the plant trypanosomatid: Phytomonas sp. isolated from Euphorbia characias.

Abstract:

:There is no effective chemotherapy against diseases caused by Phytomonas sp., a plant trypanosomatid responsible for economic losses in major crops. We tested three triazolo-pyrimidine complexes [two with Pt(II), and another with Ru(III)] against promastigotes of Phytomonas sp. isolated from Euphorbia characias. The incorporation of radiolabelled precursors, ultrastructural alterations and changes in the pattern of metabolite excretion were examined. Different degrees of toxicity were found for each complex: the platinum compound showed an inhibition effect on nucleic acid synthesis, provoking alterations on the levels of mitochondria, nucleus and glycosomes. These results, together with others reported previously in our laboratory about the activity of pyrimidine derivatives, reflect the potential of these compounds as agents in the treatment of Phytomonas sp.

journal_name

Mem Inst Oswaldo Cruz

authors

Magán R,Marín C,Salas JM,Barrera-Pérez M,Rosales MJ,Sánchez-Moreno M

doi

10.1590/s0074-02762004000600021

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

651-6

issue

6

eissn

0074-0276

issn

1678-8060

pii

S0074-02762004000600021

journal_volume

99

pub_type

杂志文章
  • RS virus diagnosis: comparison of isolation, immunofluorescence and enzyme immunoassay.

    abstract::Two techniques for rapid diagnosis, immunofluorescence (IFAT) and enzyme immunoassay (EIA), have been compared with virus isolation in tissue culture for the detection of respiratory syncytial virus (RSV) in specimens of nasopharyngeal secretions. The specimens were obtained from children under five years of age suffe...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761986000200013

    authors: Siqueira MM,Ferreira V,Nascimento JP

    更新日期:1986-04-01 00:00:00

  • Experimental infection of Calomys callosus (Rodentia, Cricetidae) by Toxoplasma gondii.

    abstract::Calomys callosus, Rengger 1830 (Rodentia, Cricetidae), a wild rodent found in Central Brazil, was studied to investigate its susceptibility to Toxoplasma gondii experimental infection and its humoral immune response against this protozoa. The electrophoretic profile of the serum proteins of C. callosus showed that IgG...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761998000100018

    authors: Favoreto-Júnior S,Ferro EA,Clemente D,Silva DA,Mineo JR

    更新日期:1998-01-01 00:00:00

  • Ultrasound monitoring of structural urinary tract disease in Schistosoma haematobium infection.

    abstract::A major advance in our understanding of the natural history of Schistosoma haematobium-related morbidity has come through the introduction of the portable ultrasound machines for non-invasive examination of the kidneys and bladder. With the use of generators or battery packs to supply power in non-clinical field setti...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000900028

    authors: King CH

    更新日期:2002-01-01 00:00:00

  • Access to diagnosis and treatment of Chagas disease/infection in endemic and non-endemic countries in the XXI century.

    abstract::In this article, Médicos Sin Fronteras (MSF) Spain faces the challenge of selecting, piecing together, and conveying in the clearest possible way, the main lessons learnt over the course of the last seven years in the world of medical care for Chagas disease. More than two thousand children under the age of 14 have be...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762007005000081

    authors: Villa L,Morote S,Bernal O,Bulla D,Albajar-Vinas P

    更新日期:2007-10-30 00:00:00

  • Association of NR1I2 gene polymorphisms and time of progression to AIDS.

    abstract:BACKGROUND:The time of progression towards AIDS can vary greatly among seropositive patients, and may be associated with host genetic variation. The NR1I2 (PXR) gene, a ligand-activated transcription factor, regulates the transcription immune pathway genes and can therefore be targets of viral replication mechanisms in...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160382

    authors: Medeiros RM,Menti CF,Benelli JL,Matte MC,Melo MG,Almeida SE,Fiegenbaum M

    更新日期:2017-04-01 00:00:00

  • An outbreak of diarrhoea associated with rotavirus serotype 1 in a day care nursery in Rio de Janeiro, Brazil.

    abstract::Faeces from 17 children less than 1.6 years old and 15 adults more than 22 years old were collected during an outbreak of gastroenteritis in a day care nursery and screened for the presence of adenovirus and rotavirus by enzyme immunoassay (EIARA) and other viruses by electron microscopy (EM) and polyacrylamide gel el...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761994000100002

    authors: de Castro L,Rodrigues Ddos P,Flauzino R,Moura M,Leite JP

    更新日期:1994-01-01 00:00:00

  • Life cycle, feeding and defecation patterns of Rhodnius ecuadoriensis (Lent & León 1958) (Hemiptera: Reduviidae: Triatominae) under laboratory conditions.

    abstract::Rhodnius ecuadoriensis is the second most important vector of Chagas Disease (CD) in Ecuador. The objective of this study was to describe (and compare) the life cycle, the feeding and defecation patterns under laboratory conditions of two populations of this specie [from the provinces of Manabí (Coastal region) and Lo...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762008000700011

    authors: Villacís AG,Arcos-Terán L,Grijalva MJ

    更新日期:2008-11-01 00:00:00

  • [Microbial resistance of Salmonella agona from various regions of Brazil].

    abstract::The object of the investigation was the evaluation of the susceptibility to antibiotic and chemotherapeutic agents of 240 strains of Salmonella agona isolated from different sources (human, food and environment) obtained from five Brazilian states (Minas Gerais, São Paulo, Rio de Janeiro, Pernambuco and Rio Grande do ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761986000100002

    authors: Solari CA,dos Reis EM,Dias JC,Hofer E

    更新日期:1986-01-01 00:00:00

  • DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis.

    abstract::Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of the Leptospira genus. Vaccination with bacterins has severe limitations. Here, we evaluated the N-terminal region of the leptospiral immunoglobulin-like B protein (LigBrep) as a vaccine candidate against leptospirosis using immunisation strategies...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760150222

    authors: Forster KM,Hartwig DD,Oliveira TL,Bacelo KL,Schuch R,Amaral MG,Dellagostin OA

    更新日期:2015-12-01 00:00:00

  • Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection.

    abstract::Direct smear examination using Ziehl-Neelsen staining for pulmonary tuberculosis (PTB) diagnosis is inexpensive and easy to use, but has the major limitation of low sensitivity. Rapid molecular methods are becoming more widely available in centralized laboratories, but they depend on timely reporting of results and st...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762011000200013

    authors: Michelon CT,Rosso F,Schmid KB,Sperhacke RD,Oliveira MM,Kritski AL,Rezende L Jr,Costa ER,Ribeiro AW,Verza M,Cafrune PI,Silva MS,Kuhleis D,Zaha A,Rossetti ML

    更新日期:2011-03-01 00:00:00

  • Use of isozyme patterns in the identification of Biomphalaria tenagophila (D'Orbigny, 1835) and B. occidentalis (Paraense, 1981) (Gastropoda: Planorbidae).

    abstract::Two sibling species of Biomphalaria, B. tenagophila and B. occidentalis were identified using isozyme patterns obtained by horizontal gel electrophoresis. Six diagnostic enzymatic loci were identified in digestive gland homogenates. The results enable us to distinguish the species, calculate the Nei's coefficient of g...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761995000300010

    authors: Mascara D,Morgante JS

    更新日期:1995-05-01 00:00:00

  • Urban malaria in the Brazilian Western Amazon Region I: high prevalence of asymptomatic carriers in an urban riverside district is associated with a high level of clinical malaria.

    abstract::Cross sectional studies on malaria prevalence was performed in 2001, 2002, and 2004 in Vila Candelária, an urban riverside area of Porto Velho, Rondônia, in the Brazilian Western Amazon, followed by longitudinal surveys on malaria incidence. Vila Candelária is a working class district, provided with electricity, water...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762007005000012

    authors: Tada MS,Marques RP,Mesquita E,Dalla Martha RC,Rodrigues JA,Costa JD,Pepelascov RR,Katsuragawa TH,Pereira-da-Silva LH

    更新日期:2007-06-01 00:00:00

  • Isolation and identification of actin-binding proteins in Plasmodium falciparum by affinity chromatography.

    abstract::The invasion of the erythrocyte by Plasmodium falciparum depends on the ability of the merozoite to move through the membrane invagination. This ability is probably mediated by actin dependent motors. Using affinity columns with G-actin and F-actin we isolated actin binding proteins from the parasite. By immunoblottin...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762000000300007

    authors: Forero C,Wasserman M

    更新日期:2000-05-01 00:00:00

  • Temporal and spatial distribution of Leishmania mexicana infections in a population of Neotoma micropus.

    abstract::A 19-month mark-release-recapture study of Neotoma micropus with sequential screening for Leishmania mexicana was conducted in Bexar County, Texas, USA. The overall prevalence rate was 14.7% and the seasonal prevalence rates ranged from 3.8 to 26.7%. Nine incident cases were detected, giving an incidence rate of 15.5/...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762003000200002

    authors: Raymond RW,McHugh CP,Witt LR,Kerr SF

    更新日期:2003-03-01 00:00:00

  • Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures.

    abstract::Three Yersinia pestis strains isolated from humans and one laboratory strain (EV76) were grown in rich media at 28 degrees C and 37 degrees C and their outer membrane protein composition compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Several proteins with molecular weights ranging f...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761990000100018

    authors: Abath FG,de Almeida AM,Ferreira LC

    更新日期:1990-01-01 00:00:00

  • Detection and characterization of rotavirus G and P types from children participating in a rotavirus vaccine trial in Belém, Brazil.

    abstract::This study sought the characterization of rotaviruses in a trial with a tetravalent rhesus-human rotavirus vaccine in Belém, Brazil in children who received three doses of vaccine or placebo in the 1st, 3rd and 5th months of life. Rotavirus electropherotypes, subgroups, G serotypes, G, [P] and [P], G genotypes were de...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1590/s0074-02762002000100020

    authors: Mascarenhas JD,Linhares AC,Gabbay YB,Leite JP

    更新日期:2002-01-01 00:00:00

  • Two new species of nematodes (Rhabditida: Diplogasteridae and Rhabditidae) parasites of Gryllodes laplatae (Orthoptera: Gryllidae) in Argentina.

    abstract::Cephalobium magdalensis n. sp. (Rhabditida: Diplogasteridae) found in Magdalena, Buenos Aires, and Cruznema lincolnensis n. sp. (Rhabditida: Rhabditidae) found in Lincoln, Buenos Aires, parasitizing the cricket Gryllodes laplatae (Orthoptera: Gryllidae) are described and illustrated. C. magdalensis n. sp. is character...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761998000600013

    authors: Reboredo GR,Camino NB

    更新日期:1998-11-01 00:00:00

  • The in vitro activity of fatty diamines and amino alcohols against mixed amastigote and trypomastigote Trypanosoma cruzi forms.

    abstract::Four diamines and three amino alcohols derived from 1-decanol, 1-dodecanol and 1,2-dodecanediol were evaluated in an in vitro assay against a mixture of trypomastigote and intracellular amastigote forms of Trypanosoma cruzi. Two of these compounds (6 and 7) showed better activity against both proliferative stages of T...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276130496

    authors: Sales Júnior PA,Rezende Júnior CO,Le Hyaric M,Almeida MV,Romanha AJ

    更新日期:2014-06-01 00:00:00

  • Redescription of Anopheles (Nyssorhynchus) antunesi Galvão & Amaral and description of a new species of the Myzorhynchella Section (Diptera: Culicidae) from Serra da Mantiqueira, Brazil.

    abstract::Anopheles (Nyssorhynchus) pristinus Nagaki & Sallum, n. sp. of the Myzorhynchella Section is described based on morphological characters of adult females, males, fourth-instar larvae, pupae and male genitalia. Anopheles (Nyssorhynchus) antunesi Galvão & Amaral is characterized to fix its identity and distinguish it fr...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762010000300006

    authors: Nagaki SS,Motta Mde A,Sallum MA

    更新日期:2010-05-01 00:00:00

  • N-terminal amino acid sequences of the major outer membrane proteins from a Neisseria meningitidis group B strain isolated in Brazil.

    abstract::The four dominant outer membrane proteins (46, 38, 33 and 28 kDa) were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in a semi-purified preparation of vesicle membranes of a Neisseria meningitidis (N44/89, B:4:P1.15:P5.5,7) strain isolated in Brazil. The N-terminal amino acid sequenc...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761996000100020

    authors: De Simone SG,Soares SA,Souza AL,Danelli MG

    更新日期:1996-01-01 00:00:00

  • Dengue virus type 3 isolation from Aedes aegypti in the municipality of Nova Iguaçu, State of Rio de Janeiro.

    abstract::In a prospective field study conducted from July 2000 to June 2001, adult Aedes aegypti and Ae. albopictus mosquitoes were caught from the municipality of Nova Iguaçu, State of Rio de Janeiro, Brazil. Virus isolation in Ae. albopictus clone C6/36 cell line and a semi-nested reverse transcription-polymerase chain react...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000600009

    authors: Lourenco-de-Oliveira R,Honorio NA,Castro MG,Schatzmayr HG,Miagostovich MP,Alves JC,Silva WC,Leite PJ,Nogueira RM

    更新日期:2002-09-01 00:00:00

  • Ivermectin resistant and susceptible third-stage larvae of Haemonchus contortus: cholinesterase and phosphatase activities.

    abstract::Cholinesterase and acid phosphatase (AP), but not alkaline phosphatase activities, were detected in cytosolic and membrane-bound fractions of ivermectin resistant and susceptible Haemonchus contortus infective-stage larvae. Some differences in acetylcholinesterase activity of cytosolic fractions and in the AP activity...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762004000200019

    authors: Giménez-Pardo C,Martínez-Grueiro MM,Gómez-Barrio A,Rodríguez-Caabeiro F

    更新日期:2004-03-01 00:00:00

  • Host characteristics do not affect community structure of ectoparasites on the fishing bat Noctilio leporinus (L., 1758) (Mammalia: Chiroptera).

    abstract::Patterns of parasite abundance and prevalence are thought to be influenced by several host characteristics such as size, sex, developmental stage, and seasonality. We examined two obligatory ectoparasites of the bat Noctilio leporinus (L.) (Chiroptera, Noctilionidae) to test whether prevalence and abundance of Noctili...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762003000600017

    authors: Moura MO,Bordignon MO,Graciolli G

    更新日期:2003-09-01 00:00:00

  • Assessing key safety concerns of a Wolbachia-based strategy to control dengue transmission by Aedes mosquitoes.

    abstract::Mosquito-borne diseases such as dengue fever, chikungunya or malaria affect millions of people each year and control solutions are urgently needed. An international research program is currently being developed that relies on the introduction of the bacterial endosymbiont Wolbachia pipientis into Aedes aegypti to cont...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762010000800002

    authors: Popovici J,Moreira LA,Poinsignon A,Iturbe-Ormaetxe I,McNaughton D,O'Neill SL

    更新日期:2010-12-01 00:00:00

  • Lectin receptors distribution in the surface membrane of Trypanosoma cruzi blood forms collected from mice submitted to specific treatment.

    abstract::The authors investigated the distribution of lectin receptors on Trypanosoma cruzi blood forms collected from mice inoculated with, respectively, the drug-resistant and drug-sensitive strains VL-10 and CL, and treated with the two standard active nitroheterocyclic compounds nifurtimox and benznidazole used for treatme...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761991000300003

    authors: Fontes G,Romanha AJ,Pereira ME,Brener Z

    更新日期:1991-07-01 00:00:00

  • Genetic an antigenic analysis of adenovirus type 3 strains showing intermediate behavior in standard seroneutralization test.

    abstract::During an epidemiological survey of acute respiratory infection in Rio de Janeiro, among 208 adenovirus isolates, we found two strains that we were not able, by a standard neutralization procedure, to distinguish between type 3 or 7. However, DNA restriction pattern for the two strains with different enzymes were anal...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761998000200019

    authors: Moraes MT,da Silva M,Leite JP,Nascimento JP

    更新日期:1998-03-01 00:00:00

  • Analgesic activity of a triterpene isolated from Scoparia dulcis L. (Vassourinha).

    abstract::Analgesic and anti-inflammatory activities of water (WE) and ethanolic (EE) extracts of Scoparia dulcis L. were investigated in rats and mice, and compared to the effects induced by Glutinol, a triterpene isolated by purification of EE. Oral administration (p.o.) of either WE or EE (up to 2 g/kg) did not alter the nor...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761991000600034

    authors: Freire SM,Torres LM,Roque NF,Souccar C,Lapa AJ

    更新日期:1991-01-01 00:00:00

  • Catadiscus pomaceae sp. n. (Trematoda, Paramphistomatidae) from Pomacea canaliculata (Lamarck, 1801) (Prosobranchia, Ampullariidae).

    abstract::Catadiscus pomaceae sp. n. from the intestine of the prosobranch mollusc Pomacea canaliculata (Lamarck, 1801), is described. The host snail was collected from a lenitic biotope belonging to the Riachuelo basin (Corrientes province, Argentina) during 1985-1986. So far the species of the genus Catadiscus Cohn, 1904 have...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761992000100002

    authors: Hamann MI

    更新日期:1992-01-01 00:00:00

  • alpha-glycerophosphate dehydrogenase activity in flight muscles of triatomine bugs Panstrongylus megistus and Triatoma sordida.

    abstract::The alpha-glycerophosphate dehydrogenase (alpha-GPDH) activity in flight muscles of Panstrongylus megistus and Triatoma sordida, vectors of Chagas disease in Brazil, was studied. Both species showed higher enzymatic activities in fliers than in non-fliers insects. T. sordida exhibited a higher proportion of flier inse...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762000000500016

    authors: Soares RP,Santoro MM

    更新日期:2000-09-01 00:00:00

  • Allelic diversity at the merozoite surface protein-1 (MSP-1) locus in natural Plasmodium falciparum populations: a brief overview.

    abstract::The merozoite surface protein-1 (MSP-1) locus of Plasmodium falciparum codes for a major asexual blood-stage antigen currently proposed as a major malaria vaccine candidate. The protein, however, shows extensive polymorphism, which may compromise its use in sub-unit vaccines. Here we compare the patterns of allelic di...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761998000500013

    authors: Ferreira MU,Kaneko O,Kimura M,Liu Q,Kawamoto F,Tanabe K

    更新日期:1998-09-01 00:00:00