Ivermectin resistant and susceptible third-stage larvae of Haemonchus contortus: cholinesterase and phosphatase activities.


:Cholinesterase and acid phosphatase (AP), but not alkaline phosphatase activities, were detected in cytosolic and membrane-bound fractions of ivermectin resistant and susceptible Haemonchus contortus infective-stage larvae. Some differences in acetylcholinesterase activity of cytosolic fractions and in the AP activity of these fractions as well as in the response to AP inhibitors by membrane-bound fractions were detected. Data are discussed.


Mem Inst Oswaldo Cruz


Giménez-Pardo C,Martínez-Grueiro MM,Gómez-Barrio A,Rodríguez-Caabeiro F




Has Abstract


2004-03-01 00:00:00














  • Characteristics of the biological cycle of Lutzomyia evandroi Costa Lima & Antunes, 1936 (Diptera: Psychodidae) under experimental conditions.

    abstract::Lutzomyia evandroi Costa Lima and Antunes, 1936 is found in Rio Grande do Norte, northeastern Brazil, in areas of visceral and mucocutaneous leishmaniasis and follows the same geographic distribution of L. longipalpis. The biological cycle, oviposition, morphological and behavioral characteristics of the species were ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: de Melo Ximenes Mde F,Maciel JC,Jerônimo SM

    更新日期:2001-08-01 00:00:00

  • An experimental and clinical assay with ketoconazole in the treatment of Chagas disease.

    abstract::Ketoconazole, an azole antifungic drug which is already in the market has also been demonstrated to be active against Trypanosoma cruzi experimental infections. In this paper we confirmed the drug effect and investigated its range of activity against different T. cruzi strains naturally resistant or susceptible to bot...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 临床试验,杂志文章


    authors: Brener Z,Cançado JR,Galvão LM,da Luz ZM,Filardi Lde S,Pereira ME,Santos LM,Cançado CB

    更新日期:1993-01-01 00:00:00

  • Human chronic chagasic cardiopathy: participation of parasite antigens, subsets of lymphocytes, cytokines and microvascular abnormalities.

    abstract::This article tries to demonstrate by new pathological findings (with the use of immunohistochemical technique and confocal laser microscopy) that chronic chagasic cardiomyopathy is a result of multiple factors involving myocarditis, immunodepression, severe fibrosis and microvessels dilatation and that all of these al...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审


    authors: de Lourdes Higuchi M

    更新日期:1999-01-01 00:00:00

  • First isolation of West Nile virus in Brazil.

    abstract:BACKGROUND:Serological evidence of West Nile virus (WNV) infection has been reported in different regions of Brazil from equine and human hosts but the virus had never been isolated in the country. OBJECTIVES:We sought to identify the viral etiology of equine encephalitis in Espírito Santo state. METHODS:We performed...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Martins LC,Silva EVPD,Casseb LMN,Silva SPD,Cruz ACR,Pantoja JAS,Medeiros DBA,Martins Filho AJ,Cruz EDRMD,Araújo MTF,Cardoso JF,Cunha MACRD,Almada GL,Romano APM,Santos MGDP,Rodrigues GAP,Chiang JO,Quaresma JAS,Carvalho

    更新日期:2019-01-17 00:00:00

  • Similarity between the association factor of ribosomal subunits and the protein Stm1p from Saccharomyces cerevisiae.

    abstract::A ribosome association factor (AF) was isolated from the yeast Sacchharomyces cerevisiae. Partial amino acid sequence of AF was determined from its fragment of 25 kDa isolated by treating AF with 2-(2-nitrophenylsulfenyl)-3-methyl-3'-Bromoindolenine (BNPS-skatole). This sequence has a 86% identity to the product of th...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Correia H,Medina R,Hernández A,Bustamante E,Chakraburtty K,Herrera F

    更新日期:2004-11-01 00:00:00

  • [Action of a molluscacide (Bayluscide-SRB) from the Dynatech R/D Company in lakes of northeastern Brazil--Sergipe, Brazil].

    abstract::To study the action of molluscicide nine ponds were selected: 3 of them lying in Maruim municipality, 29 km far from north Aracaju, the State capital, and 6 ponds in Itabaianinha municipality, 118 km far from south Aracaju. This study was carried out for 16 months. Environmental parameters observed were those thought ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Rosas E,Ribeiro EC

    更新日期:1987-01-01 00:00:00

  • Ecological analysis of acari recovered from coprolites from archaeological site of northeast Brazil.

    abstract::Coprolite samples of human and animal origin from the excavations performed at the archaeological site of Furna do Estrago, at Brejo da Madre de Deus in the state of Pernambuco, Brazil and sent to the Paleoparasitology Laboratory at Escola Nacional de Saúde Pública-Fiocruz, Rio de Janeiro, were analyzed for mites. Aft...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: de Candanedo Guerra Rde M,Gazêta GS,Amorim M,Duarte AN,Serra-Freire NM

    更新日期:2003-01-01 00:00:00

  • Human and canine visceral leishmaniasis in an emerging focus in Araçuaí, Minas Gerais: spatial distribution and socio-environmental factors.

    abstract::This study aimed to analyse the spatial distribution of human (2007-2013) and canine (2013) visceral leishmaniasis (VL) in the city of Araçuaí, Minas Gerais, Brazil, and identify the socio-environmental factors related to their occurrence. The spatial distribution of human and canine cases was analysed by kernel densi...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Ursine RL,Dias JV,Morais HA,Pires HH

    更新日期:2016-07-04 00:00:00

  • In vitro and in vivo activity of meglumine antimoniate produced at Farmanguinhos-Fiocruz, Brazil, against Leishmania (Leishmania) amazonensis, L (L.) chagasi and L (Viannia) braziliensis.

    abstract::The leishmanicidal activity of four batches of meglumine antimoniate, produced in Farmanguinhos-Fiocruz, Brazil (TAMs), was assessed and compared to Glucantime-Aventis Pharma Ltda. Using the amastigote-like in vitro model, the active concentrations of Sb v varied from 10microg/ml to 300microg/ml for L. (L.) chagasi an...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Morais-Teixeira Ed,Carvalho AS,Costa JC,Duarte SL,Mendonça JS,Boechat N,Rabello A

    更新日期:2008-06-01 00:00:00

  • Survival of tubercle bacilli in heat-fixed and stained sputum smears.

    abstract::We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputu...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Cardoso CL,Giacomelli LR,Helbel C,Sant'Ana JJ,Martins FM,Barreto AM

    更新日期:2001-02-01 00:00:00

  • Culicidae (Diptera) selection of humans, chickens and rabbits in three different environments in the province of Chaco, Argentina.

    abstract::Studies were conducted to determine the selection of humans, chickens and rabbits by Culicidae in three different environments in the province of Chaco, Argentina. Mosquitoes were collected fortnightly using cylindrical metal traps containing animal bait (chickens and rabbits). The mosquitoes were collected between Ju...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Stein M,Zalazar L,Willener JA,Almeida FL,Almirón WR

    更新日期:2013-08-01 00:00:00

  • Human toxocariasis: a seroepidemiological survey in schoolchildren of Sorocaba, Brazil.

    abstract::A seroepidemiological survey for toxocariasis, among 180 schoolchildren of the public schools of Sorocaba City, state of São Paulo, Brazil, was carried out from August 2000 to July 2001. ELISA test was performed using excretory and secretory antigens for the detection of IgG anti-Toxocara antibodies. Information regar...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Coelho LM,Silva MV,Dini CY,Giacon Neto AA,Novo NF,Silveira EP

    更新日期:2004-10-01 00:00:00

  • Draft genome of the Leptospira interrogans strains, Acegua, RCA, Prea, and Capivara, obtained from wildlife maintenance hosts and infected domestic animals.

    abstract::In the present paper, we announce new draft genomes of four Leptospira interrogans strains named Acegua, RCA, Prea, and Capivara. These strains were isolated in the state of Rio Grande do Sul, Brazil, from cattle, dog, Brazilian guinea pig, and capybara, respectively. ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Kremer FS,Eslabão MR,Jorge S,Oliveira NR,Labonde J,Santos MN,Monte LG,Grassmann AA,Cunha CE,Forster KM,Moreno LZ,Moreno AM,Campos VF,McBride AJ,Pinto LS,Dellagostin OA

    更新日期:2016-04-01 00:00:00

  • Deep sequencing of small RNAs reveals the repertoire of miRNAs and piRNAs in Biomphalaria glabrata.

    abstract:BACKGROUND:Biomphalaria glabrata snails are widely distributed in schistosomiasis endemic areas like America and Caribe, displaying high susceptibility to infection by Schistosoma mansoni. After the availability of B. glabrata genome and transcriptome data, studies focusing on genetic markers and small non-coding RNAs ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Queiroz FR,Portilho LG,Jeremias WJ,Babá ÉH,do Amaral LR,Silva LM,Coelho PMZ,Caldeira RL,Gomes MS

    更新日期:2020-07-01 00:00:00

  • Daily pattern of flight activity of Aedes albifasciatus in central Argentina.

    abstract::Aedes albifasciatus is an important common mosquito in Central Argentina. It is a confirmed vector of the Western Equine Encephalitis and is responsible for loss of milk production in dairy cattle during peak populations. This paper reports the flight activity pattern of Ae. albifasciatus for different seasons, in the...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Almeida FF,Gorla DE

    更新日期:1995-09-01 00:00:00

  • Comparative genomics reveals diverse capsular polysaccharide synthesis gene clusters in emerging Raoultella planticola.

    abstract::Raoultella planticola is an emerging zoonotic pathogen that is associated with rare but life-threatening cases of bacteremia, biliary tract infections, and urinary tract infections. Moreover, increasing antimicrobial resistance in the organism poses a potential threat to public health. In spite of its importance as a ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Huang YT,Chuang WY,Ho BC,Wu ZY,Kuo RC,Ko M,Liu PY

    更新日期:2018-08-27 00:00:00

  • Studies on the virulence and attenuation of Trypanosoma cruzi using immunodeficient animals.

    abstract::Tissue invasion and pathology by Trypanosoma cruzi result from an interaction between parasite virulence and host immunity. Successive in vivo generations of the parasite select populations with increasing ability to invade the host. Conversely, prolonged in vitro selection of the parasite produces attenuated sublines...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Basombrío MA,Segura MA,Gómez L,Padilla M

    更新日期:2000-01-01 00:00:00

  • Helicobacter pylori cagA and vacA genotypes in Cuban and Venezuelan populations.

    abstract::The aim of this study was to determine the presence of Helicobacter pylori cytotoxin-associated gene (cagA)/vacuolating cytotoxin gene (vacA) among patients with chronic gastritis in Cuba and Venezuela. Gastric antrum biopsies were taken for culture, DNA extraction and PCR analysis. Amplification of vacA and cagA segm...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Ortiz-Princz D,Guariglia-Oropeza V,Avila M,Correnti M,Perrone M,Gutierrez B,Torres J,Megraud F,Cavazza ME

    更新日期:2010-05-01 00:00:00

  • Previous dengue or Zika virus exposure can drive to infection enhancement or neutralisation of other flaviviruses.

    abstract:BACKGROUND:Dengue virus (DENV) has circulated in Brazil for over 30 years. During this time, one serotype has cyclically replaced the other, until recently, when all four distinct serotypes began to circulate together. Persistent circulation of DENV for long time periods makes sequential infections throughout a person'...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Oliveira RA,de Oliveira-Filho EF,Fernandes AI,Brito CA,Marques ET,Tenório MC,Gil LH

    更新日期:2019-01-01 00:00:00

  • Epidemiological and clinical features of Leishmania (Viannia) braziliensis American cutaneous and mucocutaneous leishmaniasis in the State of Espírito Santo, Brazil.

    abstract::Between 1985 and 2000, epidemiological surveys of the American tegumentary leishmaniasis (ATL) were carried out in several rural and urban communities in Espírito Santo, Brazil. A total of 100 stocks of Leishmania (comprising isolates from both human and canine hosts with ATL) were identified by two methods of molecul...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Falqueto A,Sessa PA,Ferreira AL,Vieira VP,Santos CB,Varejão JB,Cupolillo E,Porrozzi R,Carvalho-Paes LE,Grimaldi Júnior G

    更新日期:2003-12-01 00:00:00

  • Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni.

    abstract::The extent of genomic variability of clones of Schistosoma mansoni obtained from field isolates was compared with that of strains that have been laboratory maintained. Analysis was undertaken using randomly amplified polymorphic DNAs (RAPDs) generated with three primers. Phenograms showing the similarity among the clo...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Pinto PM,Brito CF,Passos LK,Tendler M,Simpson AJ

    更新日期:1997-05-01 00:00:00

  • Life cycle, feeding and defecation patterns of Rhodnius ecuadoriensis (Lent & León 1958) (Hemiptera: Reduviidae: Triatominae) under laboratory conditions.

    abstract::Rhodnius ecuadoriensis is the second most important vector of Chagas Disease (CD) in Ecuador. The objective of this study was to describe (and compare) the life cycle, the feeding and defecation patterns under laboratory conditions of two populations of this specie [from the provinces of Manabí (Coastal region) and Lo...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Villacís AG,Arcos-Terán L,Grijalva MJ

    更新日期:2008-11-01 00:00:00

  • The vitellogenin gene family of Aedes aegypti.

    abstract::We have been interested in identifying genes that play a role in reproduction of the mosquito Aedes aegypti. Our interests are currently focused on the vitellogenin genes which in the mosquito are expressed only in the fat body in response to the insect steroid hormone, 20-hydroxyecdysone. Four of the five vitellogeni...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Hamblin MT,Marx JL,Wolfner MF,Hagedorn HH

    更新日期:1987-01-01 00:00:00

  • The first record of Lutzomyia longipalpis in the Argentine northwest.

    abstract::In 2004, the urban presence of Lutzomyia longipalpis was recorded for the first time in Formosa province. In 2006, the first autochthonous case of human urban visceral leishmaniasis (VL) was recorded in Misiones in the presence of the vector, along with some canine VL cases. After this first case, the vector began to ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Bravo AG,Quintana MG,Abril M,Salomón OD

    更新日期:2013-12-01 00:00:00

  • The retained capacity of Lutzomyia longipalpis (Lutz & Neiva) to transmit Leishmania chagasi (Cunha & Chagas) after eight years (64 generations) in a closed laboratory colony.

    abstract::A closed Lutzomyia longipalpis colony, from Ceará has been used to transmit Leishmania chagasi isolated from a fox in Pará state. The last time this colony was successfully used in similar transmission experiments was eight years (64 generations) ago indicating that this colony of Lu. longipalpis has fully maintained ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Gonçalves MD,Ryan L,Lainson R,Shaw JJ

    更新日期:1985-07-01 00:00:00

  • Scanning electron microscopic study of Prosorhynchoides arcuatus (Linton, 1990) (Bucephalidae: Digenea).

    abstract::Prosorhynchoides arcuatus (Linton, 1900) from the intestine of Pomatomus saltator (L.) from the Atlantic coast of the State of Rio de Janeiro is studied by scanning electron microscopy, with detailed description of tegumental spines. Comments on the synonymy of this species with Bucephalopsis callicotyle Kohn, 1962 ar...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Cohen SC,Kohn A,Barth OM

    更新日期:1995-01-01 00:00:00

  • Performance of diagnostic biomarkers in predicting liver fibrosis among hepatitis C virus-infected Egyptian children.

    abstract::The aim of the present study was to identify specific markers that mirror liver fibrosis progression as an alternative to biopsy when biopsy is contraindicated, especially in children. After liver biopsies were performed, serum samples from 30 hepatitis C virus (HCV) paediatric patients (8-14 years) were analysed and ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Nassef YE,Shady MM,Galal EM,Hamed MA

    更新日期:2013-11-01 00:00:00

  • Chagas disease: national survey of seroprevalence in children under five years of age conducted in 2008.

    abstract:BACKGROUND:Since the early 1990s, programs to control Chagas disease in South America have focused on eradicating domiciliary Triatoma infestans, the main vector. Seroprevalence studies of the chagasic infection are included as part of the vector control programs; they are essential to assess the impact of vector contr...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Russomando G,Cousiño B,Sanchez Z,Franco LX,Nara EM,Chena L,Martínez M,Galeano ME,Benitez L

    更新日期:2017-05-01 00:00:00

  • Recent advances and prospective researches on molecular epidemiology of dengue viruses.

    abstract::The determination of amino acid changes in the envelope protein by direct sequencing of either genomic RNA or PCR-amplified cDNA fragments provides useful informations for assessing the genetic variability and the geographic distribution of the actually most widespread dengue-2 serotype. The possible link of variation...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审


    authors: Deubel V

    更新日期:1992-01-01 00:00:00

  • Sativa seeds against Schistosoma mansoni different stages.

    abstract::The schistosomicidal properties of Nigella sativa seeds were tested in vitro against Schistosoma mansoni miracidia, cercariae, and adult worms. Results indicate its strong biocidal effects against all stages of the parasite and also showed an inhibitory effect on egg-laying of adult female worms. In the present work w...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章


    authors: Mohamed AM,Metwally NM,Mahmoud SS

    更新日期:2005-04-01 00:00:00