DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis.

Abstract:

:Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of the Leptospira genus. Vaccination with bacterins has severe limitations. Here, we evaluated the N-terminal region of the leptospiral immunoglobulin-like B protein (LigBrep) as a vaccine candidate against leptospirosis using immunisation strategies based on DNA prime-protein boost, DNA vaccine, and subunit vaccine. Upon challenge with a virulent strain ofLeptospira interrogans, the prime-boost and DNA vaccine approaches induced significant protection in hamsters, as well as a specific IgG antibody response and sterilising immunity. Although vaccination with recombinant fragment of LigBrep also produced a strong antibody response, it was not immunoprotective. These results highlight the potential of LigBrep as a candidate antigen for an effective vaccine against leptospirosis and emphasise the use of the DNA prime-protein boost as an important strategy for vaccine development.

journal_name

Mem Inst Oswaldo Cruz

authors

Forster KM,Hartwig DD,Oliveira TL,Bacelo KL,Schuch R,Amaral MG,Dellagostin OA

doi

10.1590/0074-02760150222

subject

Has Abstract

pub_date

2015-12-01 00:00:00

pages

989-95

issue

8

eissn

0074-0276

issn

1678-8060

pii

S0074-02762015005026101

journal_volume

110

pub_type

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