Strain specific variation of outer membrane proteins of wild Yersinia pestis strains subjected to different growth temperatures.

Abstract:

:Three Yersinia pestis strains isolated from humans and one laboratory strain (EV76) were grown in rich media at 28 degrees C and 37 degrees C and their outer membrane protein composition compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Several proteins with molecular weights ranging from 34 kDa to 71 kDa were observed to change in relative abundance in samples grown at different temperatures. At least seven Y. pestis outer membrane proteins showed a temperature-dependent and strain-specific behaviour. Some differences between the outer membrane proteins of full-pathogenic wild isolates and the EV76 strain could also be detected and the relevance of this finding on the use of laboratory strains as a reference to the study of Y. pestis biological properties is discussed.

journal_name

Mem Inst Oswaldo Cruz

authors

Abath FG,de Almeida AM,Ferreira LC

doi

10.1590/s0074-02761990000100018

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

107-11

issue

1

eissn

0074-0276

issn

1678-8060

pii

S0074-02761990000100018

journal_volume

85

pub_type

杂志文章
  • First description of Leishmania (Viannia) infection in Evandromyia saulensis, Pressatia sp. and Trichophoromyia auraensis (Psychodidae: Phlebotominae) in a transmission area of cutaneous leishmaniasis in Acre state, Amazon Basin, Brazil.

    abstract::Studies on the sandfly fauna to evaluate natural infection indexes are still limited in the Brazilian Amazon, a region with an increasing incidence of cutaneous leishmaniasis. Here, by using a multiplex polymerase chain reaction directed to Leishmania kDNA and hybridisation, we were able to identify L. (Viannia) subge...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160283

    authors: Araujo-Pereira T,Pita-Pereira D,Boité MC,Melo M,Costa-Rego TA,Fuzari AA,Brazil RP,Britto C

    更新日期:2017-01-01 00:00:00

  • Clinical forms of Trypanosoma cruzi infected individuals in the chronic phase of Chagas disease in Puebla, Mexico.

    abstract::In Mexico, despite the relatively high seroprevalence of Trypanosoma cruzi infection in humans in some areas, reported morbidity of Chagas disease is not clear. We determined clinical stage in 71 individuals seropositive to T. cruzi in the state of Puebla, Mexico, an area endemic for Chagas disease with a reported ser...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762006000700005

    authors: Sánchez-Guillén Mdel C,López-Colombo A,Ordóñez-Toquero G,Gomez-Albino I,Ramos-Jimenez J,Torres-Rasgado E,Salgado-Rosas H,Romero-Díaz M,Pulido-Pérez P,Pérez-Fuentes R

    更新日期:2006-11-01 00:00:00

  • New approaches for the control and eradication of schistosomiasis in Venezuela.

    abstract::Schistosomiasis in America with the exception of Brazil, behaves as a chronic mild disease with few clinical manifestations due to low parasite burden. These features restrict the clinical and parasitological diagnosis. The most commonly used stool examination method, Kato-Katz, becomes insensitive when the majority o...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02761992000800035

    authors: de Noya BA,Noya O,Balzan C,Cesari IM

    更新日期:1992-01-01 00:00:00

  • Leptoconops nosopheris sp. n. (Diptera: Ceratopogonidae) and Paleotrypanosoma burmanicus gen. n., sp. n. (Kinetoplastida: Trypanosomatidae), a biting midge--trypanosome vector association from the Early Cretaceous.

    abstract::Leptoconops nosopheris sp. n. (Diptera: Ceratopogonidae) is described from a blood-filled female biting midge in Early Cretaceous Burmese amber. The new species is characterized by a very elongate terminal flagellomere, elongate cerci, and an indistinct spur on the metatibia. This biting midge contained digenetic tryp...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762008000500010

    authors: Poinar G Jr

    更新日期:2008-08-01 00:00:00

  • Molecular approaches for eco-epidemiological studies of Paracoccidioides brasiliensis.

    abstract::Medical mycology has greatly benefited from the introduction of molecular techniques. New knowledge on molecular genetics has provided both theoretical and practical frameworks, permitting important advances in our understanding of several aspects of pathogenic fungi. Considering Paracoccidioides brasiliensis in parti...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02762009000400018

    authors: Richini-Pereira VB,Bosco Sde M,Theodoro RC,Macoris SA,Bagagli E

    更新日期:2009-07-01 00:00:00

  • Identification of human T-lymphotropic virus type I (HTLV-I) subtypes using restricted fragment length polymorphism in a cohort of asymptomatic carriers and patients with HTLV-I-associated myelopathy/tropical spastic paraparesis from São Paulo, Brazil.

    abstract::Although human T-lymphotropic virus type I (HTLV-I) exhibits high genetic stability, as compared to other RNA viruses and particularly to human immunodeficiency virus (HIV), genotypic subtypes of this human retrovirus have been characterized in isolates from diverse geographical areas. These are currently believed not...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000300009

    authors: Segurado AA,Biasutti C,Zeigler R,Rodrigues C,Damas CD,Jorge ML,Marchiori PE

    更新日期:2002-04-01 00:00:00

  • Is the thymus a target organ in infectious diseases?

    abstract::The thymus is a central lymphoid organ, in which T cell precursors differentiate and generate most of the so-called T cell repertoire. Along with a variety of acute infectious diseases, we and others determined important changes in both microenvironmental and lymphoid compartments of the organ. For example, one major ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02761992000900010

    authors: Savino W,de Moraes Mdo C,Barbosa SD,Da Fonseca EC,De Almeida VC,Hontebeyrie-Joscowicz M

    更新日期:1992-01-01 00:00:00

  • Acute Chagas disease in the state of Pará, Amazon Region: is it increasing?

    abstract::Acute Chagas disease (ACD) has a distinct epidemiological profile in the Amazon Region, with cases and outbreaks of Trypanosoma cruzi infection being possibly related to the ingestion of contaminated food. Data on ACD in the state of Pará retrieved from 2000 to 2016 from the Brazilian Notifiable Diseases Information S...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760170298

    authors: Santos VRCD,Meis J,Savino W,Andrade JAA,Vieira JRDS,Coura JR,Junqueira ACV

    更新日期:2018-01-01 00:00:00

  • Ecogenetics of Triatoma sordida and Triatoma guasayana (Hemiptera: reduviidae) in the Bolivian chaco.

    abstract::Triatoma guasayana and two putative cryptic species pertaining to T. sordida complex (named groups 1 and 2) occur in sympatry in the Bolivian Chaco. Using multilocus enzyme electrophoresis and subsequent genetic analysis, our work assesses their population distribution and dispersal capacity in domestic, peridomestic,...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/S0074-02761999000400004

    authors: Noireau F,Gutierrez T,Flores R,Breniere F,Bosseno MF,Wisnivesky-Colli C

    更新日期:1999-07-01 00:00:00

  • Sandfly species diversity in association with human activities in the Kani tribe settlements of the Western Ghats, Thiruvananthapuram, Kerala, India.

    abstract::Sandfly prevalence in the Kani tribe settlements of Western Ghats in India was investigated. A total of 1,279 sandflies comprising 17 species was obtained. Sandfly abundance showed a negative correlation (r = -0.97, p = 0.003) with increase in altitudinal ranges from 0-1,000 m. When sandfly samples were grouped accord...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760140272

    authors: Ranganathan S,Swaminathan S

    更新日期:2015-04-01 00:00:00

  • Conservation and developmental expression of ubiquitin isopeptidases in Schistosoma mansoni.

    abstract::Several genes related to the ubiquitin (Ub)-proteasome pathway, including those coding for proteasome subunits and conjugation enzymes, are differentially expressed during the Schistosoma mansoni life cycle. Although deubiquitinating enzymes have been reported to be negative regulators of protein ubiquitination and sh...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276130107

    authors: Pereira RV,Vieira HG,Oliveira VF,Gomes Mde S,Passos LK,Borges Wde C,Guerra-Sá R

    更新日期:2014-02-01 00:00:00

  • Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells.

    abstract::Acute infection with Trypanosoma cruzi results in intense myocarditis, which progresses to a chronic, asymptomatic indeterminate form. The evolution toward this chronic cardiac form occurs in approximately 30% of all cases of T. cruzi infection. Suppression of delayed type hypersensitivity (DTH) has been proposed as a...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276108062013004

    authors: Thé TS,Portella RS,Guerreiro ML,Andrade SG

    更新日期:2013-09-01 00:00:00

  • Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil.

    abstract:BACKGROUND:Enterocytozoon bieneusi are the most common microsporidia associated with different clinical manifestations such as diarrhoea, respiratory tract inflammation and acalculous cholecystitis, especially in immunocompromised patients. Infection usually occurs by ingestion of food and water contaminated with spore...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160435

    authors: Yamashiro S,Fiuza VRDS,Teixeira ÂTLS,Branco N,Levy CE,Castro ICVS,Franco RMB

    更新日期:2017-06-01 00:00:00

  • The in vitro activity of fatty diamines and amino alcohols against mixed amastigote and trypomastigote Trypanosoma cruzi forms.

    abstract::Four diamines and three amino alcohols derived from 1-decanol, 1-dodecanol and 1,2-dodecanediol were evaluated in an in vitro assay against a mixture of trypomastigote and intracellular amastigote forms of Trypanosoma cruzi. Two of these compounds (6 and 7) showed better activity against both proliferative stages of T...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276130496

    authors: Sales Júnior PA,Rezende Júnior CO,Le Hyaric M,Almeida MV,Romanha AJ

    更新日期:2014-06-01 00:00:00

  • [Dispersion of Biomphalaria straminea in the State of Sergipe: a comparative study within an interval of 19 years].

    abstract::The aim of the current paper was to study in the state of Sergipe the population distribution and interactions between two species of Biomphalaria, the snail vectors of Schistosoma mansoni in the northeastern Brazil. Data collected in 1969 showed that B. straminea, with only one exception, was limited to the semi-dry ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761989000300014

    authors: Figueiredo CC

    更新日期:1989-07-01 00:00:00

  • Preliminary analysis of Sm14 in distinct fractions of Schistosoma mansoni adult worm extract.

    abstract::In previous studies it was shown that the recombinant molecule, r-Sm14, induces high levels of protection against Schistosoma mansoni infection in two outbred animal models and immune crossprotection against infection by Fasciola hepatica in Swiss outbred mice. r-Sm14 was derived from a living worm extract, called SE,...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762001000900011

    authors: Thaumaturgo N,Vilar MM,Diogo CM,Edelenyi R,Tendler M

    更新日期:2001-01-01 00:00:00

  • Association of NR1I2 gene polymorphisms and time of progression to AIDS.

    abstract:BACKGROUND:The time of progression towards AIDS can vary greatly among seropositive patients, and may be associated with host genetic variation. The NR1I2 (PXR) gene, a ligand-activated transcription factor, regulates the transcription immune pathway genes and can therefore be targets of viral replication mechanisms in...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160382

    authors: Medeiros RM,Menti CF,Benelli JL,Matte MC,Melo MG,Almeida SE,Fiegenbaum M

    更新日期:2017-04-01 00:00:00

  • Genetic an antigenic analysis of adenovirus type 3 strains showing intermediate behavior in standard seroneutralization test.

    abstract::During an epidemiological survey of acute respiratory infection in Rio de Janeiro, among 208 adenovirus isolates, we found two strains that we were not able, by a standard neutralization procedure, to distinguish between type 3 or 7. However, DNA restriction pattern for the two strains with different enzymes were anal...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761998000200019

    authors: Moraes MT,da Silva M,Leite JP,Nascimento JP

    更新日期:1998-03-01 00:00:00

  • Criteria for identification of Schistosoma mansoni eggs in faecal sediments prepared with the Helmintex method and stained by ninhydrin.

    abstract::Helmintex is a sensitive method used for detecting Schistosoma mansoni eggs. Here, we describe the observed frequency of six proposed criteria associated with the identification of S. mansoni eggs prepared with the Helmintex method and stained with ninhydrin. The efficacy of these criteria in classifying S. mansoni eg...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760180529

    authors: de Souza RP,Favero V,Pascoal VF,Lindholz C,Bittencourt HR,Graeff-Teixeira C

    更新日期:2019-01-01 00:00:00

  • A cladistic analysis of Inaequalium (Coscarón & Wygodzinsky, 1984), with information on geographical distribution (Diptera: Simuliidae).

    abstract::The black flies of the genus Inaequalium present a Neotropical distribution, with Panama at the northern limit, and the Argentinian pampas at the southern, but do not occur in the Central Amazon. This study offers a cladistic analysis establishing a hypothesis of relationships between the species of Inaequalium. A tot...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000100010

    authors: Strieder MN,Py-Daniel V

    更新日期:2002-01-01 00:00:00

  • Urban Chagas disease in children and women in primary care centres in Buenos Aires, Argentina.

    abstract::The primary objective of this study was to estimate the prevalence of this disease in women of childbearing age and children treated at health centres in underserviced areas of the city of Buenos Aires. Demographic and Chagas disease status data were collected. Samples for Chagas disease serology were obtained on filt...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,多中心研究

    doi:10.1590/0074-02760150107

    authors: Moscatelli G,Berenstein A,Tarlovsky A,Siniawski S,Biancardi M,Ballering G,Moroni S,Schwarcz M,Hernández S,García-Bournissen F,Cozzi AE,Freilij H,Altcheh J

    更新日期:2015-08-01 00:00:00

  • [Microecological characterization of parasitic nematoda present in goats of arid zones of Venezuela].

    abstract::Goats from the Venezuelan northern arid zones were found infested by the following nematodes: Haemonchus contortus, Trichostrongylus axei, T. colubriformis, Cooperia curticei, Oesophagostomum columbianum, Skrjabinema ovis y Trichuris globulosa. The Shannon-Weaver index of diversity values oscillated between 0.045 and ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761986000200009

    authors: Morales G,Pino LA,Aldana E,Perdomo L,Molina E

    更新日期:1986-04-01 00:00:00

  • Liaisons dangereuses: cross-border gene flow and dispersal of insecticide resistance-associated genes in the mosquito Aedes aegypti from Brazil and French Guiana.

    abstract:BACKGROUND:In recent years, South America has suffered the burden of continuous high impact outbreaks of dengue, chikungunya and Zika. Aedes aegypti is the main mosquito vector of these arboviruses and its control is the only solution to reduce transmission. OBJECTIVES:In order to improve vector control it is essentia...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760190120

    authors: Salgueiro P,Restrepo-Zabaleta J,Costa M,Galardo AKR,Pinto J,Gaborit P,Guidez A,Martins AJ,Dusfour I

    更新日期:2019-09-23 00:00:00

  • Diversity of Campylobacter isolates from three activated sludge systems.

    abstract::Thermophilic campylobacters were isolated from three sewage plants in Rio de Janeiro, RJ, Brazil and identified. Laboratory analysis of 390 sewage samples showed the presence of 169 thermophilic strains. The results demonstrated that human and animal pathogenic biotypes could be isolated from activated sludge during t...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761998000300003

    authors: Lauria-Filgueiras AL,Hofer E

    更新日期:1998-05-01 00:00:00

  • Polysaccharide-rich fraction of Agaricus brasiliensis enhances the candidacidal activity of murine macrophages.

    abstract::A polysaccharide-rich fraction (ATF) of medicinal mushroom Agaricus brasiliensis was evaluated on the candidacidal activity, H2O2 and nitric oxide (NO) production, and expression of mannose receptors by murine peritoneal macrophages. Mice received three intraperitoneal (i.p.) injections of ATF and after 48 h their per...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762008005000011

    authors: Martins PR,Gameiro MC,Castoldi L,Romagnoli GG,Lopes FC,Pinto AV,Loyola W,Kaneno R

    更新日期:2008-05-01 00:00:00

  • In vitro susceptibility of Plasmodium falciparum Welch field isolates to infusions prepared from Artemisia annua L. cultivated in the Brazilian Amazon.

    abstract::Artemisinin is the active antimalarial compound obtained from the leaves of Artemisia annua L. Artemisinin, and its semi-synthetic derivatives, are the main drugs used to treat multi-drug-resistant Plasmodium falciparum (one of the human malaria parasite species). The in vitro susceptibility of P. falciparum K1 and 3d...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762012000700004

    authors: Silva LF,Magalhães PM,Costa MR,Alecrim Md,Chaves FC,Hidalgo Ade F,Pohlit AM,Vieira PP

    更新日期:2012-11-01 00:00:00

  • Comparison of two PCR methods for detection of Leptospira interrogans in formalin-fixed and paraffin-embedded tissues.

    abstract::In this study we compared two polymerase chain reaction (PCR) methods using either 16S ribosomal RNA (rRNA) or 23S rRNA gene primers for the detection of different Leptospira interrogans serovars. The performance of these two methods was assessed using DNA extracted from bovine tissues previously inoculated with sever...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762012000100012

    authors: D'Andrea A,Martinez YZ,Alduina R,Monteverde V,Molina CF,Vitale M

    更新日期:2012-02-01 00:00:00

  • Increased levels of reactive oxygen species in platelets and platelet-derived microparticles and the risk of respiratory failure in HIV/AIDS patients.

    abstract::Respiratory failure (RF) is the main cause of hospital admission in HIV/AIDS patients. This study assessed comorbidities and laboratory parameters in HIV/AIDS inpatients with RF (N = 58) in relation to those without RF (N = 36). Tuberculosis showed a huge relative risk and platelet counts were slightly higher in HIV/A...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760200082

    authors: Gama WM,Oliveira LB,Chaves YO,Ribeiro F,Almeida TVR,Baptista BJA,Santana MF,Ferreira LC,Lacerda MVG,Nogueira PA

    更新日期:2020-09-14 00:00:00

  • Fossil psychodoid flies and their relation to parasitic diseases.

    abstract::Psychodid sand flies are blood-sucking fly vectors of several parasitic diseases. The oldest definitive record of this group is from the Lower Cretaceous amber of Lebanon (circa-135 to -125 My), but the high diversity within this group supports the idea that the psychodoids originated much earlier in history. The pala...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762003000900007

    authors: Azar D,Nel A

    更新日期:2003-01-01 00:00:00

  • Report of Didymocystis wedli Ariola, 1902 (Digenea; Didymozoidae) from Thunnus albacares in Brazil.

    abstract::Didymocystis wedli a parasite from the gills of Thunnus albacares from the coast of the State of Rio de Janeiro, is described by use of light and scanning electron microscopy. This is the first report of this species in Brazil and South America. New data are presented on the surface topography as demonstrated by scann...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762001000700011

    authors: Kohn A,Santos A,Baptista-Farias MF

    更新日期:2001-10-01 00:00:00