Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro.

abstract：:In the present paper, we announce new draft genomes of four Leptospira interrogans strains named Acegua, RCA, Prea, and Capivara. These strains were isolated in the state of Rio Grande do Sul, Brazil, from cattle, dog, Brazilian guinea pig, and capybara, respectively. ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Kremer FS,Eslabão MR,Jorge S,Oliveira NR,Labonde J,Santos MN,Monte LG,Grassmann AA,Cunha CE,Forster KM,Moreno LZ,Moreno AM,Campos VF,McBride AJ,Pinto LS,Dellagostin OA

更新日期：2016-04-01 00:00:00

abstract：:Several factors appear to affect vertical HIV-1 transmission, dependent mainly on characteristics of the mother (extent of immunodeficiency, co-infections, risk behaviour, nutritional status, immune response, genetical make-up), but also of the virus (phenotype, tropism) and, possibly, of the child (genetical make-up)...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Bongertz V

更新日期：2001-01-01 00:00:00

abstract：:Two essential oils of Lippia alba (Mill.) N.E. Brown (Verbenacea), the carvone and citral chemotypes and 15 of their compounds were evaluated to determine cytotoxicity and antifungal activity. Cytotoxicity assays for both the citral and carvone chemotypes were carried out with tetrazolium-dye, which showed a dose-depe...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Mesa-Arango AC,Montiel-Ramos J,Zapata B,Durán C,Betancur-Galvis L,Stashenko E

更新日期：2009-09-01 00:00:00

abstract：:Cytokines are important in the cell-mediated response to Schistosoma mansoni eggs. We have found that Th2 cytokine responses (e.g. IL-4 and IL-5) are augmented after egg laying begins while Th1 responses (IL-2 and IFN-gamma) are down regulated in S. mansoni infected mice. Treatment of mice with anti-IL-5 monoclonal an...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Cheever AW,Xu Y,Macedonia JG,Cox T,Hieny S,Sher A

更新日期：1992-01-01 00:00:00

abstract：:A shift from large to small average sizes was observed in Triatoma infestans and Rhodnius domesticus between field and domestic (or laboratory) conditions of life. It was more pronounced in the female specimens, leading to a subsequent reduction of sexual size dimorphism. This feature is discussed in terms of genetic ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Dujardin J,Steinden M,Chavez T,Machane M,Schofield C

更新日期：1999-07-01 00:00:00

abstract：:Faeces from 17 children less than 1.6 years old and 15 adults more than 22 years old were collected during an outbreak of gastroenteritis in a day care nursery and screened for the presence of adenovirus and rotavirus by enzyme immunoassay (EIARA) and other viruses by electron microscopy (EM) and polyacrylamide gel el...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： de Castro L,Rodrigues Ddos P,Flauzino R,Moura M,Leite JP

更新日期：1994-01-01 00:00:00

abstract：:This was a retrospective descriptive study on a series of cases of schistosomal myeloradiculopathy (SMR) and the aim was to investigate the incidence of this disease and its clinical and epidemiological characteristics in cases diagnosed at three healthcare units in Pernambuco, Brazil between 1994-2006. The data were ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Araújo KC,Silva Cda R,Santos AG,Barbosa CS,Ferrari TC

更新日期：2010-07-01 00:00:00

abstract：:BACKGROUND Chagas disease, resulting from Trypanosoma cruzi infections, continues to be a health concern mainly in Latin American countries where the parasite is endemic. The laboratory diagnosis of a chronic infection is determined through serological assays for antibodies against T. cruzi and several tests are avail...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Sáez-Alquezar A,Junqueira ACV,Durans ADM,Guimarães AV,Corrêa JA,Provance DW Jr,Cabello PH,Coura JR,Viñas PA

更新日期：2020-01-01 00:00:00

abstract：:The cellular nature of the infiltrate in cutaneous lesion of rhesus monkeys experimentally infected with Leishmania (L.) amazonensis was characterized by immunohistochemistry. Skin biopsies from infected animals with active or healing lesions were compared to non-infected controls (three of each type) to quantitate in...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Amaral V,Pirmez C,Gonçalves A,Ferreira V,Grimaldi G Jr

更新日期：2000-03-01 00:00:00

abstract：:Hepatosplenic schistosomiasis was the first human disease in which the possibility of extensive long standing hepatic fibrosis being degraded and removed has been demonstrated. When such changes occurred, the main signs of portal hypertension (splenomegaly, esophageal varices) progressively disappeared, implying that ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Andrade ZA,Baptista AP,Santana TS

更新日期：2006-09-01 00:00:00

abstract：:The epidemiological features of rotavirus A (RVA) infection differ between children from developing and developed countries which could result in differences in vaccine efficacy around the world. To evaluate the impact of Rotarix™ on RVA prevalence, we monitored RVA genotypes circulating in Goiânia by monitoring virus...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Borges AM,Dias e Souza M,Fiaccadori FS,Cardoso Dd

更新日期：2011-06-01 00:00:00

abstract：:The alpha-glycerophosphate dehydrogenase (alpha-GPDH) activity in flight muscles of Panstrongylus megistus and Triatoma sordida, vectors of Chagas disease in Brazil, was studied. Both species showed higher enzymatic activities in fliers than in non-fliers insects. T. sordida exhibited a higher proportion of flier inse...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Soares RP,Santoro MM

更新日期：2000-09-01 00:00:00

abstract：:Lymphatic filariasis caused by nematode parasites Wuchereria bancrofti or Brugia malayi is a spectral disease and produces wide range of immune responses and varying levels of microfilaraemia in infected individuals. The relationship between the immune response of host and the developmental stage of the parasite as we...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Athisaya Mary K,Hoti S,Paily K

更新日期：2006-05-01 00:00:00

abstract：:In this work, a murine experimental model of toxocariasis has been developed in BALB/c, C57BL/10 and C3H murine strains orally inoculated with 4,000 Toxocara canis embryonated eggs, in order to investigate the isotype-specific immune responses against excretory-secretory antigens from larvae. T. canis specific IgG+M, ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Cuéllar C,Fenoy S,del Aguila C,Guillén JL

更新日期：2001-05-01 00:00:00

abstract：:The study was set up to evaluate the impact of two commercial larvicide formulations, Bacillus thuringiensis serovar israelensis base (Bti) at 15 ppm/1 min and temephos at 0.03 ppm of active ingredient, used to control Simulium pertinax populations, on associated non-target entomofauna occupying the same breeding site...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： de Araújo-Coutinho CJ,Cunha Ade B,Serra-Freire NM,de Mello RP

更新日期：2003-07-01 00:00:00

abstract：:Trypanosoma rangeli, a parasite generally considered non-pathogenic for man, is the second species of human trypanosome to be reported from the New World. The geographical distribution of T. rangeli often overlaps with that of T. cruzi, the same vertebrate and invertebrate hosts being infected. Their differentiation t...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Guhl F,Vallejo GA

更新日期：2003-06-01 00:00:00

abstract：:Rhesus monkeys (Macaca mulatta) were infected subcutaneously with 1.0 x 10(4) to 1.5 x 10(4) metacyclic trypomastigotes of Trypanosoma cruzi (Colombian strain). Parasitological and immunological parameters were evaluated in these animals for periods of 1 month to over 3 years. A chagoma was observed between the 3rd an...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Bonecini-Almeida Mda G,Galvão-Castro B,Pessoa MH,Pirmez C,Laranja F

更新日期：1990-04-01 00:00:00

abstract：:Six clinical isolates of influenza A viruses were examined for hemagglutinin receptor specificity and neuraminidase substrate specificity. All of the viral isolates minimally passaged in mammalian cells demonstrated preferential agglutination of human erythrocytes enzymatically modified to contain NeuAc alpha 2,6Gal s...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Couceiro JN,Baum LG

更新日期：1994-10-01 00:00:00

abstract：:Here, we observed the uptake of membrane-impermeant molecules by cercariae as they penetrate the skin and are transformed into schistosomula. We propose that membrane-impermeant molecules, Lucifer Yellow, Propidium iodide and Hoechst 33258 enter the parasite through both thenephridiopore and the surface membrane and t...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Thornhill J,Kusel J,Oliviera FA,Ribeiro F,Lima SF,Coelho PM,McVeigh P,Mattos AC

更新日期：2010-07-01 00:00:00

abstract：BACKGROUND:Cutaneous leishmaniasis (CL) generally presents with a single or several localised cutaneous ulcers without involvement of mucous membranes. Ulcerated lesions are susceptible to secondary contamination that may slow the healing process. OBJECTIVE:This study verified the influence of non-parasitic wound infe...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Antonio LF,Lyra MR,Saheki MN,Schubach AO,Miranda LFC,Madeira MF,Lourenço MCDS,Fagundes A,Ribeiro ÉADS,Barreto L,Pimentel MIF

更新日期：2017-09-01 00:00:00

abstract：:Analysis of zymograms with SDS-polyacrilamide gel electrophoresis containing gelatin as substrate, and performed on samples of haemolymph or fat body taken from Rhodnius prolixus inoculated or not with Enterobacter cloacae, demonstrated distinct patterns of protease activities: (i) in the haemolymph two proteases were...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Feder D,Salles JM,Garcia ES,Azambuja P

更新日期：1998-11-01 00:00:00

abstract：:Feline coprolites were examined for parasites with the aim of studying ancient infections that occurred in the Patagonian region during the Holocene period. Eggs compatible to Trichuris sp., Calodium sp., Eucoleus sp., Nematodirus sp., Oesophagostomum sp. (Nematoda), Monoecocestus sp. (Cestoda) and Eimeria macusaniens...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Fugassa MH,Beltrame MO,Bayer MS,Sardella NH

更新日期：2009-12-01 00:00:00

abstract：:Acinetobacter baumannii is a strictly aerobic bacterium which causes severe infections, however its pathogenic characteristics are not well defined. Thirteen A. baumannii strains isolated from urine of hospitalized and nonhospitalized patients with different ages were investigated for the presence of virulence factors...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Braun G,Vidotto MC

更新日期：2004-12-01 00:00:00

abstract：:Studies on natural infection by Leishmania spp of sandflies collected in endemic and nonendemic areas can provide important information on the distribution and intensity of the transmission of these parasites. This study sought to investigate the natural infection by Leishmaniain wild female sandflies. The specimens w...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Oliveira EF,Casaril AE,Mateus NL,Murat PG,Fernandes WS,Oshiro ET,Oliveira AG,Galati EA

更新日期：2015-12-01 00:00:00

abstract：:Penicillin tolerance among 67 strains of beta-hemolytic streptococci was examined by determining the ratio of the minimal bactericidal concentration to the minimal inhibitory concentration as 32 or greater. Tolerance was demonstrated in 15 group A strains and in 11.7, and 4 of groups B, C and G, respectively. Thereaft...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Avelino CC,Benchetrit LC

更新日期：1995-07-01 00:00:00

abstract：:Direct smear examination using Ziehl-Neelsen staining for pulmonary tuberculosis (PTB) diagnosis is inexpensive and easy to use, but has the major limitation of low sensitivity. Rapid molecular methods are becoming more widely available in centralized laboratories, but they depend on timely reporting of results and st...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Michelon CT,Rosso F,Schmid KB,Sperhacke RD,Oliveira MM,Kritski AL,Rezende L Jr,Costa ER,Ribeiro AW,Verza M,Cafrune PI,Silva MS,Kuhleis D,Zaha A,Rossetti ML

更新日期：2011-03-01 00:00:00

abstract：:A new species of planorbid mollusc, Plesiophysa dolichomastix (Greek dolichos = long, mastix = flagellum), collected from Lagoa da Pedra, municipality of Santa Rosa, state of Goiás, Brazil (15 degrees 01'S, 47 degrees 13'W) is described. It is indistinguishable by the shell characters from the five congeneric species ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Paraense WL

更新日期：2002-06-01 00:00:00

abstract：:This paper presents the morphological, histological and ultrastructural characteristics of Myxobolus oliveirai sp. nov., a parasite of the gill filaments in Brycon hilarii from the Brazilian Pantanal. Out of 216 B. hilariispecimens examined (126 wild and 90 cultivated), 38.1% of wild specimens (n = 48) were infected. ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Milanin T,Eiras JC,Arana S,Maia AA,Alves AL,Silva MR,Carriero MM,Ceccarelli PS,Adriano EA

更新日期：2010-09-01 00:00:00

abstract：:Mycobacteria, specially Mycobacterium tuberculosis are among the micro-organisms that are increasing dramatically the number of infections with death, all over the world. A great number of animal experimental models have been proposed to investigate the mechanisms involved in the host response against these intracellu...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Menezes-de-Lima-Júnior O,Henriques M das G

更新日期：1997-01-01 00:00:00

abstract：:Angiotensin II (AII), a product of rennin-angiotensin system, exerts an important role on the function of immune system cells. In this study, the effect of AII on the phagocytic activity of mouse peritoneal macrophages was assessed. Mice peritoneal macrophages were cultured for 48 h and the influence of different conc...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Belline P,da Melo PS,Haun M,Palhares FB,Boer PA,Gontijo JA,Figueiredo JF

更新日期：2004-03-01 00:00:00