Haemolymph and fat body metallo-protease associated with Enterobacter cloacae infection in the bloodsucking insect, Rhodnius prolixus.

Abstract:

:Analysis of zymograms with SDS-polyacrilamide gel electrophoresis containing gelatin as substrate, and performed on samples of haemolymph or fat body taken from Rhodnius prolixus inoculated or not with Enterobacter cloacae, demonstrated distinct patterns of protease activities: (i) in the haemolymph two proteases were induced in insects inoculated with bacteria; (ii) two proteases were detected in the fat bodies derived from non-inoculated controls or insect inoculated with sterile culture medium; (iii) haemolymph and fat body had both the same apparent molecular weights proteases (46 and 56 kDa); and (iv) these enzymes were characterized as metallo-proteases. The association of these enzymes in Rhodnius infected with bacteria was discussed.

journal_name

Mem Inst Oswaldo Cruz

authors

Feder D,Salles JM,Garcia ES,Azambuja P

doi

10.1590/s0074-02761998000600023

subject

Has Abstract

pub_date

1998-11-01 00:00:00

pages

823-6

issue

6

eissn

0074-0276

issn

1678-8060

pii

S0074-02761998000600023

journal_volume

93

pub_type

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