Phenotypic and genotypic characteristics of shiga toxin-producing Escherichia coli strains isolated from children in São Paulo, Brazil.

Abstract:

:The biochemical and serological characteristics, virulence properties, and genetic relatedness of Shiga toxin-producing Escherichia coli (STEC) strains isolated in S o Paulo, from April 1989 through March 1990, were determined. This is also the first report on clinic findings of human STEC infections in Brazil. The only three STEC strains identified in that period were lysine decarboxylase negative, belonged to serotype O111ac: non-motile, were Stx1 producers, carried the eae and astA genes, and 2 of them also presented the EHEC-hly sequence. The children carrying STEC were all boys, with less than two years old, and had no previous history of hospitalization. None of them presented blood in stools. Vomiting, cough and coryza were the most common clinical manifestations observed. Although the STEC strains were isolated during summer months, and presented similar phenotypic and genotypic characteristics, carbohydrate fermentation patterns and PFGE analysis suggested that these diarrheal episodes were not caused by a single clone.

journal_name

Mem Inst Oswaldo Cruz

authors

Guth BE,Ramos SR,Cerqueira AM,Andrade JR,Gomes TA

doi

10.1590/s0074-02762002000800003

subject

Has Abstract

pub_date

2002-12-01 00:00:00

pages

1085-9

issue

8

eissn

0074-0276

issn

1678-8060

pii

S0074-02762002000800003

journal_volume

97

pub_type

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