Human papillomavirus type distribution and HPV16 intratype diversity in southern Brazil in women with and without cervical lesions.

Abstract:

BACKGROUND:Increasing evidence suggests that human papillomavirus (HPV) intratype variants (specific lineages and sublineages) are associated with pathogenesis and progression from HPV infection to persistence and the development of cervical cancer. OBJECTIVES:This study aimed to verify the prevalence of HPV infection and distribution of HPV types and HPV16 variants in southern Brazil in women with normal cytology or intraepithelial lesions. METHODS:HPV typing was determined by L1 gene sequencing. To identify HPV16 variants, the LCR and E6 regions were sequenced, and characteristic single nucleotide variants were identified. FINDINGS:A total of 445 samples were studied, with 355 from cervical scrapes and 90 from cervical biopsies. HPV was detected in 24% and 91% of these samples, respectively. The most prevalent HPV types observed were 16 (cervical, 24%; biopsies, 57%) and 58 (cervical, 12%; biopsies, 12%). Seventy-five percent of the HPV16-positive samples were classified into lineages, with 88% defined as lineage A, 10% as lineage D, and 2% as lineage B. MAIN CONCLUSIONS:This study identified a high frequency of European and North American HPV16 lineages, consistent with the genetic background of the human population in southern Brazil.

journal_name

Mem Inst Oswaldo Cruz

authors

de Oliveira GR,Vieira VC,Ávila EC,Finger-Jardim F,Caldeira TD,Gatti FA,Gonçalves CV,Oliveira SG,da Hora VP,Soares MA,de Martinez AM

doi

10.1590/0074-02760160530

subject

Has Abstract

pub_date

2017-07-01 00:00:00

pages

492-498

issue

7

eissn

0074-0276

issn

1678-8060

pii

S0074-02762017000700492

journal_volume

112

pub_type

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