Sensitivity of a vacuum aspiratory culture technique for diagnosis of localized cutaneous leishmaniasis in an endemic area of Leishmania (Viannia) braziliensis transmission.

Abstract:

:Sixty eight patients with localized cutaneous leishmaniasis from an area with Leishmania (Viannia) braziliensis transmission had cultures performed with a modified Marzochís vacuum aspiratory puncture technique to establish sensitivity and contamination rate with this new method. Overall sensitivity of three aspirates was 47.1%; (CI95% 39.4; 59.4) significantly greater than the sensitivity of a single one aspirate. Fungal contamination was observed in 6/204 (2.9%) inoculated culture tubes. We recommend that this useful technique should be adopted as routine for primary isolation of L. (V.) braziliensis from localized cutaneous ulcers.

journal_name

Mem Inst Oswaldo Cruz

authors

Romero GA,Sampaio RN,Macedo Vde O,Marsden PD

doi

10.1590/s0074-02761999000400014

subject

Has Abstract

pub_date

1999-07-01 00:00:00

pages

505-8

issue

4

eissn

0074-0276

issn

1678-8060

pii

S0074-0276(99)09400414

journal_volume

94

pub_type

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