Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea).

Abstract:

:Heteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of approximately 42 kDa in the whole-cell lysates of parasites targeting mammals (T. cruzi, Trypanosoma brucei and Leishmania major), insects (Angomonas deanei, Crithidia fasciculata, Herpetomonas samuelpessoai and Strigomonas culicis) and plants (Phytomonas serpens). A single polypeptide of approximately 42 kDa was detected in the whole-cell lysates of T. cruzi cultured epimastigotes, metacyclic trypomastigotes and amastigotes at similar protein expression levels. Confocal microscopy showed that actin was expressed throughout the cytoplasm of all the tested trypanosomatids. These data demonstrate that actin expression is widespread in trypanosomatids.

journal_name

Mem Inst Oswaldo Cruz

authors

Souza LC,Pinho RE,Lima CV,Fragoso SP,Soares MJ

doi

10.1590/0074-0276108052013015

subject

Has Abstract

pub_date

2013-08-01 00:00:00

pages

631-6

issue

5

eissn

0074-0276

issn

1678-8060

pii

S0074-02762013000500631

journal_volume

108

pub_type

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