Abstract:
:The nuclear phenotypes of Malpighian tubule cells in fifth instar nymphs of Triatoma infestans, one of the most important vectors of Chagas disease, were studied following sequential shocks at 0 degrees C, separated by intervals of 8 h and 24 h at 30 degrees C, under conditions of moderate fasting and full nourishment. The insects pertained to colonies reared in the laboratory and originated from domestic specimens collected in the Brazilian states of São Paulo (north) and Minas Gerais (south). Since nuclear phenotypes in this species are affected by single cold shocks, it was expected that these phenotypes could also be changed by sequential shocks. Nuclear phenotypes indicative of mechanisms of cell survival (nuclear fusion and heterochromatin decondensation) and cell death (apoptosis and necrosis) were observed concomitantly in all the conditions tested. Nuclear fusion and heterochromatin decondensation were not found relevant for the presumed acquisition of the cold-hardening response in T. infestans. The decreased frequency of apoptosis and necrosis following sequential cold shocks including under fasting conditions, indicated that tolerance to sequential cold shocks occurred in T. infestans of the mentioned origin.
journal_name
Mem Inst Oswaldo Cruzjournal_title
Memorias do Instituto Oswaldo Cruzauthors
Campos SG,Rodrigues VL,Mello MLdoi
10.1590/s0074-02762002000600019subject
Has Abstractpub_date
2002-09-01 00:00:00pages
857-64issue
6eissn
0074-0276issn
1678-8060pii
S0074-02762002000600019journal_volume
97pub_type
杂志文章abstract::The main purpose of the present study was to examine if there is difference in terms of incidence rates of congenital toxoplasmosis among populations assisted in public and private hospitals from Uberlândia, state of Minas Gerais, Brazil. A total of 805 serum samples from cord blood were collected, being 500 from publ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762004000100002
更新日期:2004-02-01 00:00:00
abstract::Cholinesterase and acid phosphatase (AP), but not alkaline phosphatase activities, were detected in cytosolic and membrane-bound fractions of ivermectin resistant and susceptible Haemonchus contortus infective-stage larvae. Some differences in acetylcholinesterase activity of cytosolic fractions and in the AP activity...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762004000200019
更新日期:2004-03-01 00:00:00
abstract::Through its life cycle from the insect vector to mammalian hosts Trypanosoma cruzi has developed clever strategies to reach the intracellular milieu where it grows sheltered from the hosts' immune system. We have been interested in several aspects of in vitro interactions of different infective forms of the parasite w...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761999000700014
更新日期:1999-01-01 00:00:00
abstract::Hepatitis B virus (HBV) has a low endemicity in Rio de Janeiro, Brazil. Sexual transmission must play an important role in this virus, but the prevalence and risk factors have never been properly investigated. The aim of this paper is to determine the prevalence and risk factors for HBV infection in patients attending...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762001000500007
更新日期:2001-07-01 00:00:00
abstract::This study was undertaken to investigate the prevalence of Mansonella ozzardi infection and to estimate the parasitic infection rate (PIR) in simuliid black flies in the municipality of Pauini, Amazonas, Brazil. We used thick blood films to examine 921 individuals in 35 riverine communities along the Pauini and Purus ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762009000100012
更新日期:2009-02-01 00:00:00
abstract::Acute cases of schistosomiasis have been found on the coastal area of Pernambuco, Brazil, due to environmental disturbances and disorderly occupation of the urban areas. This study identifies and spatially marks the main foci of the snail host species, Biomphalaria glabrata on Itamaracá Island. The chaotic occupation ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762004000900014
更新日期:2004-01-01 00:00:00
abstract::A ribosome association factor (AF) was isolated from the yeast Sacchharomyces cerevisiae. Partial amino acid sequence of AF was determined from its fragment of 25 kDa isolated by treating AF with 2-(2-nitrophenylsulfenyl)-3-methyl-3'-Bromoindolenine (BNPS-skatole). This sequence has a 86% identity to the product of th...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762004000700012
更新日期:2004-11-01 00:00:00
abstract:BACKGROUND:Enterocytozoon bieneusi are the most common microsporidia associated with different clinical manifestations such as diarrhoea, respiratory tract inflammation and acalculous cholecystitis, especially in immunocompromised patients. Infection usually occurs by ingestion of food and water contaminated with spore...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-02760160435
更新日期:2017-06-01 00:00:00
abstract::Sandfly prevalence in the Kani tribe settlements of Western Ghats in India was investigated. A total of 1,279 sandflies comprising 17 species was obtained. Sandfly abundance showed a negative correlation (r = -0.97, p = 0.003) with increase in altitudinal ranges from 0-1,000 m. When sandfly samples were grouped accord...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-02760140272
更新日期:2015-04-01 00:00:00
abstract::Acinetobacter baumannii is a strictly aerobic bacterium which causes severe infections, however its pathogenic characteristics are not well defined. Thirteen A. baumannii strains isolated from urine of hospitalized and nonhospitalized patients with different ages were investigated for the presence of virulence factors...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762004000800010
更新日期:2004-12-01 00:00:00
abstract::The high sensitivity and the possibility of automation of the enzyme-linked-immunosorbent-assay (ELISA) has indicated this technique as one of the most useful serological test for epidemiological studies. In the present study, an ELISA for detection of IgG antibodies against adult worm antigens (IgG-ELISA) was investi...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761998000700054
更新日期:1998-01-01 00:00:00
abstract::Triatoma guasayana and two putative cryptic species pertaining to T. sordida complex (named groups 1 and 2) occur in sympatry in the Bolivian Chaco. Using multilocus enzyme electrophoresis and subsequent genetic analysis, our work assesses their population distribution and dispersal capacity in domestic, peridomestic,...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/S0074-02761999000400004
更新日期:1999-07-01 00:00:00
abstract::From October 2000 to April 2001, insecticide bioassays were conducted in 18 ranches from 10 counties in the states of Mato Grosso and Mato Grosso do Sul, in Central Brazil. Horn flies from wild populations were exposed to diazinon-impregnated filter papers immediately after collection on cattle, and mortality was reco...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762002000600029
更新日期:2002-09-01 00:00:00
abstract::Several parasitological studies carried out in El Salvador between 2000-2012 showed a higher frequency of acute cases of Chagas disease than that in other Central American countries. There is an urgent need for improved Chagas disease surveillance and vector control programs in the provinces where acute Chagas disease...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-0276140416
更新日期:2014-04-01 00:00:00
abstract::A seroepidemiological survey for toxocariasis, among 180 schoolchildren of the public schools of Sorocaba City, state of São Paulo, Brazil, was carried out from August 2000 to July 2001. ELISA test was performed using excretory and secretory antigens for the detection of IgG anti-Toxocara antibodies. Information regar...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:/S0074-02762004000600002
更新日期:2004-10-01 00:00:00
abstract::Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of the Leptospira genus. Vaccination with bacterins has severe limitations. Here, we evaluated the N-terminal region of the leptospiral immunoglobulin-like B protein (LigBrep) as a vaccine candidate against leptospirosis using immunisation strategies...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-02760150222
更新日期:2015-12-01 00:00:00
abstract::Clinical and laboratory evidence is reviewed which shows that there is a great deal of variation in the susceptibility of Schistosoma mansoni to oxamniquine. This variation occurs both among endemic regions and within endemic regions in Brazil and Kenya. It is genetically controlled. It is suggested that the parasite ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761987000800026
更新日期:1987-01-01 00:00:00
abstract::A pleiotropic response to the calpain inhibitor MDL28170 was detected in the tomato parasite Phytomonas serpens. Ultrastructural studies revealed that MDL28170 caused mitochondrial swelling, shortening of flagellum and disruption of trans Golgi network. This effect was correlated to the inhibition in processing of cru...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-02760160270
更新日期:2017-01-01 00:00:00
abstract::Leptospirosis in humans usually involves hypokalaemia and hypomagnesaemia and the putative mechanism underlying such ionic imbalances may be related to nitric oxide (NO) production. We previously demonstrated the correlation between serum levels of NO and the severity of renal disease in patients with severe leptospir...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/S0074-0276108042013007
更新日期:2013-06-01 00:00:00
abstract::Several studies conducted all over the world have reported that the influenza virus is associated with great morbidity and mortality rates. In this study, we analyzed the incidence of the influenza virus between 2000 and 2003 in Curitiba. We studied 1621 samples obtained from outpatients and hospitalized patients of b...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762008000200009
更新日期:2008-03-01 00:00:00
abstract::Applications of flow cytometry to clinical and experimental hematopoietic stem cell transplantation (HSCT) are discussed in this review covering the following topics: diagnosis and classification of lymphohematologic disorders, quantitation of hematopoietic progenitors in the graft, lymphohematopoietic reconstitution ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章,评审
doi:10.1590/s0074-02762000000300018
更新日期:2000-05-01 00:00:00
abstract::A preliminary study of the pharmacokinetic parameters of t-Butylaminoethanethiol (TBAESH) was performed after administration of a single dose (35 mg/kg) either orally or intravenously. Plasma or blood samples were treated with dithiothreitol, perchloric acid and, after filtration, submitted to further purification wit...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761990000300013
更新日期:1990-07-01 00:00:00
abstract::With the currently known distribution of genera and species of the tribe Triatomini Jeannel, 1919, maps and diagrams were prepared, showing the geographical area occupied by the species and their respective dispersion in grades of latitude and altitude. Two genera are not treated: Panstrongylus Berg, 1879, already pub...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761998000100007
更新日期:1998-01-01 00:00:00
abstract::The evolutionary relationships of sand flies and Leishmania are discussed in this report, which draws distinctions between co-association, co-evolution and co-speciation (or co-cladogenesis). Examples focus on Phlebotomus vectors of Le. infantum and Le. major in the Mediterranean subregion. ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762000000400024
更新日期:2000-07-01 00:00:00
abstract::Certifying the absence of Chagas disease transmission by native vectors lacks scientific grounds and weakens long-term control-surveillance systems to the detriment of people living under risk conditions. Instead, a regular "certification of good practice" (including vector control-surveillance, case detection/patient...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-0276108022013022
更新日期:2013-04-01 00:00:00
abstract::Integration of human papillomavirus (HPV) DNA into human cells accompanied by the disruption of the viral genome has been described as a prerequisite for cancer development. This study aimed to investigate E2 gene integrity of HPV16 and HPV58 viruses isolated from infected women with cervical lesions. Forty-two HPV16-...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-02760160269
更新日期:2016-12-01 00:00:00
abstract::The cases of five patients with unusual manifestations of acute schistosomiasis mansoni are described in this paper. One patient developed skin lesions, three displayed diverse lung involvement, and one presented pyogenic liver abscesses caused by Staphylococcus aureus. ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761997000500013
更新日期:1997-09-01 00:00:00
abstract::The biochemical pathways involved in nicotinamide adenine dinucleotide (NAD) biosynthesis converge at the enzymatic step catalysed by nicotinamide mononucleotide adenylyltransferase (NMNAT, EC: 2.7.7.1). The majority of NMNATs are assembled into homo-oligomeric states that comprise 2-6 subunits. Recently, the NMNAT of...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-02760180073
更新日期:2018-07-10 00:00:00
abstract::In this transversal study, factors related to infection with and transmission of Schistosoma mansoni were explored. Based on stool examinations of two Kato-Katz smears of a single sample, the prevalences of schistosomiasis and geohelminths were established. In a multivariable analysis, sets of demographic, socio-econo...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762010000400037
更新日期:2010-07-01 00:00:00
abstract::This paper presents a list of 131 Mallophaga type specimens deposited in the Werneck Collection of Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brazil. It includes 69 holotypes, 62 allotypes, 683 paratypes, 1 syntype and 2 neoparatypes, distributed among 6 families and 35 genera. The types are listed with their respect...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762003000200012
更新日期:2003-03-01 00:00:00