Ionic imbalance and lack of effect of adjuvant treatment with methylene blue in the hamster model of leptospirosis.

Abstract:

:Leptospirosis in humans usually involves hypokalaemia and hypomagnesaemia and the putative mechanism underlying such ionic imbalances may be related to nitric oxide (NO) production. We previously demonstrated the correlation between serum levels of NO and the severity of renal disease in patients with severe leptospirosis. Methylene blue inhibits soluble guanylyl cyclase (downstream of the action of any NO synthase isoforms) and was recently reported to have beneficial effects on clinical and experimental sepsis. We investigated the occurrence of serum ionic changes in experimental leptospirosis at various time points (4, 8, 16 and 28 days) in a hamster model. We also determined the effect of methylene blue treatment when administered as an adjuvant therapy, combined with late initiation of standard antibiotic (ampicillin) treatment. Hypokalaemia was not reproduced in this model: all of the groups developed increased levels of serum potassium (K). Furthermore, hypermagnesaemia, rather than magnesium (Mg) depletion, was observed in this hamster model of acute infection. These findings may be associated with an accelerated progression to acute renal failure. Adjuvant treatment with methylene blue had no effect on survival or serum Mg and K levels during acute-phase leptospirosis in hamsters.

journal_name

Mem Inst Oswaldo Cruz

authors

Santos CS,Azevedo EC,Soares LM,Carvalho MO,dos Santos AC,das Chagas AD Jr,da Silva CL,Chagas UM,dos Reis MG,Athanazio DA

doi

10.1590/S0074-0276108042013007

subject

Has Abstract

pub_date

2013-06-01 00:00:00

pages

438-45

issue

4

eissn

0074-0276

issn

1678-8060

pii

S0074-02762013000400438

journal_volume

108

pub_type

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