Toxoplasma gondii infection induces lipid metabolism alterations in the murine host.

Abstract:

:Host lipids have been implicated in the pathogenesis of Toxoplasma gondii infection. To determine if Toxoplasma infection influences the lipid status in the normal host, we assessed serum lipids of Swiss-Webster mice during infection with the BGD-1 strain (type-2) at a series of time points. Mice were bled at days zero and 42 post-infection, and subgroups were additionally bled on alternating weeks (model 1), or sacrificed at days zero, 14 and 42 (model 2) for the measurement of total cholesterol (Chl), high density lipoproteins (HDL), low density lipoproteins (LDL) and triglycerides and adiponectin. At day 42, brains were harvested for cyst enumeration. A significant decrease (p = 0.02) in HDL and total Chl was first noted in infected vs. control mice at day 14 and persisted to day 42 (p = 0.013). Conversely, LDL was unaltered until day 42, when it increased (p = 0.043). Serum LDL levels at day 42 correlated only with cyst counts of above 300 (found in 44% mice), while the change in HDL between days zero and 42 correlated with both the overall mean cyst count (p = 0.041) and cyst counts above 300 (p = 0.044). Calculated per cyst, this decrease in HDL in individual animals ranged from 0.1-17 micromol/L, with a mean of 2.43 +/- 4.14 micromol/L. Serum adiponectin levels remained similar between infected and control mice throughout the experiment.

journal_name

Mem Inst Oswaldo Cruz

authors

Milovanović I,Vujanić M,Klun I,Bobić B,Nikolić A,Ivović V,Trbovich AM,Djurković-Djaković O

doi

10.1590/s0074-02762009000200008

subject

Has Abstract

pub_date

2009-03-01 00:00:00

pages

175-8

issue

2

eissn

0074-0276

issn

1678-8060

pii

S0074-02762009000200008

journal_volume

104

pub_type

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