More productive in vitro culture of Cryptosporidium parvum for better study of the intra- and extracellular phases.

abstract：:Although vaccines are the best means of protection against influenza, neuraminidase inhibitors are currently the main antiviral treatment available to control severe influenza cases. One of the most frequent substitutions in the neuraminidase (NA) protein of influenza A(H3N2) viruses during or soon after oseltamivir a...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Pontoriero A,Avaro M,Benedetti E,Russo M,Czech A,Periolo N,Campos A,Zamora A,Baumeister E

更新日期：2016-12-01 00:00:00

abstract：:The Brazilian variant of human immunodeficiency virus type 1 (HIV-1) subtype B, (serotype B"-GWGR), has a tryptophan replacing the proline in position 328 the HIV-1 envelope. A longer median time period from infection to acquired immunodeficiency syndrome (AIDS) for serotype B (B"-GWGR) infected subjects compared to t...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Casseb J,Montanheiro P,Komninakis S,Brito A,Duarte AJ

更新日期：2004-02-01 00:00:00

abstract：:This study's objective was to search for Cryptosporidium sp. in diarrheic feces from children aged zero to 12 years and cared for at medical units within Universidade Federal de Uberlândia or at a private practice in Uberlândia, State of Minas Gerais, Brazil, from September 1992 to August 1993. Three fecal samples pre...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Gennari-Cardoso ML,Costa-Cruz JM,de Castro E,Lima LM,Prudente DV

更新日期：1996-09-01 00:00:00

abstract：:Five field populations of Musca domestica L. collected in poultry farms were bioassayed in order to detect possible resistance to the larvicide cyromazine in Brazil. The concentrations used were 0, 0.5, 0.1, 0.2, 0.4, 1, 2, 4 and 8 ppm. Three populations (Petrópolis, RJ, Montes Claros, MG and Promissão, SP) were resis...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Pinto MC,do Prado AP

更新日期：2001-07-01 00:00:00

abstract：:A cross-sectional study for schistosomiasis was carried out in the localities of Aliança, Alegre and Coroatá (districts of Cururupu, São Bento and São João Batista, respectively) in the lowland of the state of Maranhão, after respectively 13, 11 and 4 mass treatments with oxamniquine in the period of ten years (1977-1...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Cutrim RN,Coura JR

更新日期：1992-10-01 00:00:00

abstract：:Sixteen species of ectoparasites were collected from 50 wild rodents, from August 1990 to August 1991, in an area of Araucaria augustifolia forest, in the municipality of Tijucas do Sul, State of Paraná, Brazil. Ectoparasites infested 98% of the rodents, with the highest indices of infestation found in the drycool sea...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Barros-Battesti DM,Arzua M,Linardi PM,Botelho JR,Sbalqueiro IJ

更新日期：1998-11-01 00:00:00

abstract：:Soluble antigens (Ag) from Paracoccidioides brasiliensis, Histoplasma capsulatum and Aspergillus fumigatus were prepared and standardized by double immunodiffusion (DID) and immunoelectroosmophoresis (IEOP). No difference in sensitivity was observed between the two techniques; 100% of standard patient sera were positi...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Ferreira-da-Cruz MF,Galvão-Castro B,Wanke B

更新日期：1985-07-01 00:00:00

abstract：:In this work, the susceptibility to benznidazole of two parental Trypanosoma cruzi strains, Colombian and Berenice-78, was compared to isolates obtained from dogs infected with these strains for several years. In order to evaluate the susceptibility to benznidazole two groups of mice were infected with one of five dis...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Veloso VM,Carneiro CM,Toledo MJ,Lana M,Chiari E,Tafuri WL,Bahia MT

更新日期：2001-10-01 00:00:00

abstract：:The role of intracellular free polyamine (putrescine and spermidine) pools in multiple resistance to aminoglycoside antibiotics was investigated among in vitro selected kanamycin-resistant Escherichia coli J53 mutants expressing diminished oligopeptide-binding protein (OppA) levels and/or defective ornithine decarboxy...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Acosta MB,Ferreira RC,Ferreira LC,Costa SO

更新日期：2005-11-01 00:00:00

abstract：:Sand flies were collected in the central region of the state of Rondônia (W 64 degrees 30' to 63 degrees 00' and S 10 degrees 00'to 11 degrees 00') using Shannon and CDC light traps from October 1997 to August 2000. A total of 85,850 specimens representing 78 named species were captured. Of these 14 were new records f...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Gil LH,Basano SA,Souza AA,Silva MG,Barata I,Ishikawa EA,Camargo LM,Shaw JJ

更新日期：2003-09-01 00:00:00

abstract：:We present herein an improved assay for detecting the presence of extracellular proteases from microorganisms on agar plates. Using different substrates (gelatin, BSA, hemoglobin) incorporated into the agar and varying the culture medium composition, we were able to detect proteolytic activities from Pseudomonas aerug...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Vermelho AB,Meirelles MN,Lopes A,Petinate SD,Chaia AA,Branquinha MH

更新日期：1996-11-01 00:00:00

abstract：:Experiments with Squamatoides trivitattus were carried out in two different controlled temperatures (16 +/- 1 degree C/50-60% RH and 27 +/- 1 degree C/70-80% RH). The viability of larvae and pupae at 27 degrees C was 89.82% and 92.75% respectively. Larvae did not develop at 16 degrees C. Larval development lasted for ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Salviano RJ,de Mello RP,Beck LC,d'Almeida JM

更新日期：1996-03-01 00:00:00

abstract：:Studies on natural infection by Leishmania spp of sandflies collected in endemic and nonendemic areas can provide important information on the distribution and intensity of the transmission of these parasites. This study sought to investigate the natural infection by Leishmaniain wild female sandflies. The specimens w...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Oliveira EF,Casaril AE,Mateus NL,Murat PG,Fernandes WS,Oshiro ET,Oliveira AG,Galati EA

更新日期：2015-12-01 00:00:00

abstract：:The infection pattern in Swiss mice and Triatomine bugs (Rhodnius neglectus) of eleven clones and the original stock of a Trypanosoma cruzi isolate, derived from a naturally infected Didelphis marsupialis, were biochemically and biologically characterized. The clones and the original isolate were in the same zymodeme ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Lima VS,Mangia RH,Carreira JC,Marchevsky RS,Jansen AM

更新日期：1999-05-01 00:00:00

abstract：:The lymneid snail Lymnaea bogotensis Pilsbry 1935 is synonymized with L. cousini Jousseaume 1887, based on morphological comparisons of the reproductive systems. The shell, renal tube, and reproductive system are described and illustrated from specimens collected in the type locality and the municipality of Paipa, Col...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Velásquez LE

更新日期：2006-11-01 00:00:00

abstract：:We analyzed prospectively 326 laboratory-confirmed, uncomplicated malarial infections (46.3% due to Plasmodium vivax, 35.3% due to P. falciparum, and 18.4% mixed-species infections) diagnosed in 162 rural Amazonians aged 5-73 years. Thirteen symptoms (fever, chills, sweating, headache, myalgia, arthralgia, abdominal p...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： da Silva-Nunes M,Ferreira MU

更新日期：2007-06-01 00:00:00

abstract：BACKGROUND:In Brazil, the Yellow Fever virus (YFV) is endemic in the Amazon, from where it eventually expands into epidemic waves. Coastal south-eastern (SE) Brazil, which has been a YFV-free region for eight decades, has reported a severe sylvatic outbreak since 2016. The virus spread from the north toward the south o...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Abreu FVS,Delatorre E,Dos Santos AAC,Ferreira-de-Brito A,de Castro MG,Ribeiro IP,Furtado ND,Vargas WP,Ribeiro MS,Meneguete P,Bonaldo MC,Bello G,Lourenço-de-Oliveira R

更新日期：2019-01-01 00:00:00

abstract：:Early Cretaceous flagellates with characters typical of trypanosomatids were found in the gut of sand fly larvae, as well as in surrounding debris, in Burmese amber. This discovery supports a hypothesis in which free-living trypanosomatids could have been acquired by sand fly larvae in their feeding environment and th...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Poinar G Jr

更新日期：2007-08-01 00:00:00

abstract：:Several genes related to the ubiquitin (Ub)-proteasome pathway, including those coding for proteasome subunits and conjugation enzymes, are differentially expressed during the Schistosoma mansoni life cycle. Although deubiquitinating enzymes have been reported to be negative regulators of protein ubiquitination and sh...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Pereira RV,Vieira HG,Oliveira VF,Gomes Mde S,Passos LK,Borges Wde C,Guerra-Sá R

更新日期：2014-02-01 00:00:00

abstract：:In this study, IgA1 levels in the milk and serum of puerperae were compared and a correlation was established between the levels of this immunoglobulin and the occurrence of parasitism. Eighty-three paired milk and serum samples were obtained from puerperal and IgA1 levels were analyzed. In addition, the presence of i...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Manhani MN,Cruz JM,Ferreira DM,Abdallah VO,Souza MA

更新日期：2008-08-01 00:00:00

abstract：:We report the prevalence of human papillomavirus type 16 (HPV-16) variants in women with cervical lesions from the Federal District, Central Brazil. We analyzed 34 HPV-16 samples, identifying the sequence variations of E6 and L1 genes and correlating variant frequency with disease status. The most prevalent HPV-16 var...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Cruz MR,Cerqueira DM,Cruz WB,Camara GN,Brígido MM,Silva EO,Carvalho LG,Martins CR

更新日期：2004-05-01 00:00:00

abstract：BACKGROUND:Increasing evidence suggests that human papillomavirus (HPV) intratype variants (specific lineages and sublineages) are associated with pathogenesis and progression from HPV infection to persistence and the development of cervical cancer. OBJECTIVES:This study aimed to verify the prevalence of HPV infection...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： de Oliveira GR,Vieira VC,Ávila EC,Finger-Jardim F,Caldeira TD,Gatti FA,Gonçalves CV,Oliveira SG,da Hora VP,Soares MA,de Martinez AM

更新日期：2017-07-01 00:00:00

abstract：:Faeces from 17 children less than 1.6 years old and 15 adults more than 22 years old were collected during an outbreak of gastroenteritis in a day care nursery and screened for the presence of adenovirus and rotavirus by enzyme immunoassay (EIARA) and other viruses by electron microscopy (EM) and polyacrylamide gel el...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： de Castro L,Rodrigues Ddos P,Flauzino R,Moura M,Leite JP

更新日期：1994-01-01 00:00:00

abstract：:Previous investigations showed that Schistosoma mansoni infection aggravates protein malabsorption in undernourished mice and this can be reverted by administration of casein hydrolysate. The present study was undertaken to evaluate the effects of ingestion of casein hydrolysate for long periods. Albino Swiss mice wer...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Ferreira HS,Coutinho EM,Nascimento GR,Carvalho MC

更新日期：1998-01-01 00:00:00

abstract：:The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of ...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Barbieri RR,Sales AM,Illarramendi X,Moraes MO,Nery JA,Moreira SJ,Sarno EN,Machado Ade M,Bozza FA

更新日期：2014-11-01 00:00:00

abstract：:We have initiated a gene discovery program in Schistosoma mansoni based on the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of cDNAs obtained from single passes in automatic DNA sequences. ESTs can be used to identify genes on the basis of their homology with sequences from other species deposit...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Franco GR,Simpson AJ,Pena SD

更新日期：1995-03-01 00:00:00

abstract：:The current drug options for the treatment of chronic Chagas disease have not been sufficient and high hopes have been placed on the use of genomic data from the human parasite Trypanosoma cruzi to identify new drug targets and develop appropriate treatments for both acute and chronic Chagas disease. However, the lack...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Alves-Ferreira M,Guimarães AC,Capriles PV,Dardenne LE,Degrave WM

更新日期：2009-12-01 00:00:00

abstract：:Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of the Leptospira genus. Vaccination with bacterins has severe limitations. Here, we evaluated the N-terminal region of the leptospiral immunoglobulin-like B protein (LigBrep) as a vaccine candidate against leptospirosis using immunisation strategies...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Forster KM,Hartwig DD,Oliveira TL,Bacelo KL,Schuch R,Amaral MG,Dellagostin OA

更新日期：2015-12-01 00:00:00

abstract：:The determination of amino acid changes in the envelope protein by direct sequencing of either genomic RNA or PCR-amplified cDNA fragments provides useful informations for assessing the genetic variability and the geographic distribution of the actually most widespread dengue-2 serotype. The possible link of variation...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Deubel V

更新日期：1992-01-01 00:00:00

abstract：:We describe a simple method for detection of Plasmodium vivax and Plasmodium falciparum infection in anophelines using a triplex TaqMan real-time polymerase chain reaction (PCR) assay (18S rRNA). We tested the assay on Anopheles darlingi and Anopheles stephensi colony mosquitoes fed with Plasmodium-infected blood meal...

journal_title：Memorias do Instituto Oswaldo Cruz

authors： Bickersmith SA,Lainhart W,Moreno M,Chu VM,Vinetz JM,Conn JE

更新日期：2015-06-01 00:00:00