A sensitive, specific and reproducible real-time polymerase chain reaction method for detection of Plasmodium vivax and Plasmodium falciparum infection in field-collected anophelines.

Abstract:

:We describe a simple method for detection of Plasmodium vivax and Plasmodium falciparum infection in anophelines using a triplex TaqMan real-time polymerase chain reaction (PCR) assay (18S rRNA). We tested the assay on Anopheles darlingi and Anopheles stephensi colony mosquitoes fed with Plasmodium-infected blood meals and in duplicate on field collected An. darlingi. We compared the real-time PCR results of colony-infected and field collected An. darlingi, separately, to a conventional PCR method. We determined that a cytochrome b-PCR method was only 3.33% as sensitive and 93.38% as specific as our real-time PCR assay with field-collected samples. We demonstrate that this assay is sensitive, specific and reproducible.

journal_name

Mem Inst Oswaldo Cruz

authors

Bickersmith SA,Lainhart W,Moreno M,Chu VM,Vinetz JM,Conn JE

doi

10.1590/0074-02760150031

subject

Has Abstract

pub_date

2015-06-01 00:00:00

pages

573-6

issue

4

eissn

0074-0276

issn

1678-8060

pii

S0074-02762015005050031

journal_volume

110

pub_type

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