Abstract:
:Protection against Fasciola hepatica in goats immunized with a synthetic recombinant antigen from Schistosoma mansoni fatty acid-binding protein 14 (rSm14) was investigated by assessing worm burdens, serum levels of hepatic enzymes, faecal egg count and hepatic damage, which was evaluated using gross and microscopic morphometric observation. The nature of the local immune response was assessed by examining the distribution of CD2+, CD4+, CD8+ and γ´+ T lymphocytes along with IgG+, IL-4+ and IFN-γ+ cells in the liver and hepatic lymph nodes (HLN). The goats used consisted of group 1 (unimmunized and uninfected), group 2 [infected control - immunized with Quillaia A (Quil A)] and group 3 (immunized with rSm14 in Quil A and infected), each containing seven animals. Immunization with rSm14 in Quil A adjuvant induced a reduction in gross hepatic lesions of 56.6% (p < 0.001) and reduced hepatic and HLN infiltration of CD2+, CD4+, CD8+ and γ´+ T lymphocytes as well as IL-4+ and IFN-γ+ cells (p < 0.05). This is the first report of caprine immunization against F. hepatica using a complete rSm14 molecule derived from S. mansoni. Immunization reduced hepatic damage and local inflammatory infiltration into the liver and HLN. However, considering that Quil A is not the preferential/first choice adjuvant for Sm14 immunization, further studies will be undertaken using the monophosphoryl lipid A-based family of adjuvants during clinical trials to facilitate anti-Fasciolavaccine development.
journal_name
Mem Inst Oswaldo Cruzjournal_title
Memorias do Instituto Oswaldo Cruzauthors
Mendes RE,Zafra R,Pérez-Ecija RA,Buffoni L,Martínez-Moreno A,Tendler M,Pérez Jdoi
10.1590/s0074-02762010000500017subject
Has Abstractpub_date
2010-08-01 00:00:00pages
698-705issue
5eissn
0074-0276issn
1678-8060pii
S0074-02762010000500017journal_volume
105pub_type
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journal_title:Memorias do Instituto Oswaldo Cruz
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journal_title:Memorias do Instituto Oswaldo Cruz
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journal_title:Memorias do Instituto Oswaldo Cruz
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journal_title:Memorias do Instituto Oswaldo Cruz
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journal_title:Memorias do Instituto Oswaldo Cruz
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journal_title:Memorias do Instituto Oswaldo Cruz
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journal_title:Memorias do Instituto Oswaldo Cruz
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journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
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journal_title:Memorias do Instituto Oswaldo Cruz
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