Aspidosperma (Apocynaceae) plant cytotoxicity and activity towards malaria parasites. Part I: Aspidosperma nitidum (Benth) used as a remedy to treat fever and malaria in the Amazon.

Abstract:

:Infusions of Aspidosperma nitidum (Apocynaceae) wood bark are used to treat fever and malaria in the Amazon Region. Several species of this family are known to possess indole alkaloids and other classes of secondary metabolites, whereas terpenoids, an inositol and the indole alkaloids harmane-3 acid and braznitidumine have been described in A. nitidum . In the present study, extracts from the wood bark, leaves and branches of this species were prepared for assays against malaria parasites and cytotoxicity testing using human hepatoma and normal monkey kidney cells. The wood bark extracts were active against Plasmodium falciparum and showed a low cytotoxicity in vitro, whereas the leaf and branch extracts and the pure alkaloid braznitidumine were inactive. A crude methanol extract was subjected to acid-base fractionation aimed at obtaining alkaloid-rich fractions, which were active at low concentrations against P. falciparum and in mice infected with and sensitive Plasmodium berghei parasites. Our data validate the antimalarial usefulness of A. nitidum wood bark, a remedy that can most likely help to control malaria. However, the molecules responsible for this antimalarial activity have not yet been identified. Considering their high selectivity index, the alkaloid-rich fractions from the plant bark might be useful in the development of new antimalarials.

journal_name

Mem Inst Oswaldo Cruz

authors

Coutinho JP,Aguiar AC,dos Santos PA,Lima JC,Rocha MG,Zani CL,Alves TM,Santana AE,Pereira Mde M,Krettli AU

doi

10.1590/0074-0276130246

subject

Has Abstract

pub_date

2013-12-01 00:00:00

pages

974-82

issue

8

eissn

0074-0276

issn

1678-8060

pii

S0074-02762013000800974

journal_volume

108

pub_type

杂志文章
  • Access to diagnosis and treatment of Chagas disease/infection in endemic and non-endemic countries in the XXI century.

    abstract::In this article, Médicos Sin Fronteras (MSF) Spain faces the challenge of selecting, piecing together, and conveying in the clearest possible way, the main lessons learnt over the course of the last seven years in the world of medical care for Chagas disease. More than two thousand children under the age of 14 have be...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762007005000081

    authors: Villa L,Morote S,Bernal O,Bulla D,Albajar-Vinas P

    更新日期:2007-10-30 00:00:00

  • Immune response in cattle vaccinated against rabies.

    abstract::In order to determine the best type of rabies vaccine to use as a booster, 78 serological samples from singly vaccinated cattle were analyzed by counterimmunoelectrophoresis technique. The animals were divided into several groups, received the first vaccine dose with modified live virus vaccine (ERA strain) and were r...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762000000100013

    authors: Oliveira AN,Andrade MC,Silva MV,Moura WC,Cortez Contreiras E

    更新日期:2000-01-01 00:00:00

  • [Microecological characterization of parasitic nematoda present in goats of arid zones of Venezuela].

    abstract::Goats from the Venezuelan northern arid zones were found infested by the following nematodes: Haemonchus contortus, Trichostrongylus axei, T. colubriformis, Cooperia curticei, Oesophagostomum columbianum, Skrjabinema ovis y Trichuris globulosa. The Shannon-Weaver index of diversity values oscillated between 0.045 and ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761986000200009

    authors: Morales G,Pino LA,Aldana E,Perdomo L,Molina E

    更新日期:1986-04-01 00:00:00

  • [Morbidity of schistosomiasis mansoni in Brazil. Developmental study in an endemic area over a 10-year period].

    abstract::Four cross sectional studies on morbidity of schistosomiasis mansoni were carried out respectively in 1973, 1974, 1979 and 1983 in the Village of Capitão Andrade, District of Itanhomi, State of Minas Gerais, Brazil, through clinical and fecal examination of the population. In addition a comparison was made of the dina...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761984000400009

    authors: Coura JR,Conceição MJ,Pereira JB

    更新日期:1984-10-01 00:00:00

  • Polymorphism in trypomastigotes of Trypanosoma (Megatrypanum) minasense in the blood of experimentally infected squirrel monkey and marmosets.

    abstract::Experimental infections by Trypanosoma (Megatrypanum) minasense were performed in primates - Saimiri sciureus and Callithrix penicillata - with the objective of searching for morphological variations of the blood trypomastigotes with respect to hosts and time of infection. We carried out morphological and morphometric...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761999000500016

    authors: Ziccardi M,Lourenco-de-Oliveira R

    更新日期:1999-09-01 00:00:00

  • The influence of meteorological variables on the oviposition dynamics of Aedes aegypti (Diptera: Culicidae) in four environmentally distinct areas in northeast Brazil.

    abstract::BACKGROUND Fluctuations in climate have been associated with variations in mosquito abundance. OBJECTIVES To analyse the influence of precipitation, temperature, solar radiation, wind speed and humidity on the oviposition dynamics of Aedes aegypti in three distinct environmental areas (Brasília Teimosa, Morro da Conce...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760200046

    authors: Santos ICDS,Braga C,de Souza WV,de Oliveira ALS,Regis LN

    更新日期:2020-01-01 00:00:00

  • Report of Didymocystis wedli Ariola, 1902 (Digenea; Didymozoidae) from Thunnus albacares in Brazil.

    abstract::Didymocystis wedli a parasite from the gills of Thunnus albacares from the coast of the State of Rio de Janeiro, is described by use of light and scanning electron microscopy. This is the first report of this species in Brazil and South America. New data are presented on the surface topography as demonstrated by scann...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762001000700011

    authors: Kohn A,Santos A,Baptista-Farias MF

    更新日期:2001-10-01 00:00:00

  • Acute schistosomiasis: report on five singular cases.

    abstract::The cases of five patients with unusual manifestations of acute schistosomiasis mansoni are described in this paper. One patient developed skin lesions, three displayed diverse lung involvement, and one presented pyogenic liver abscesses caused by Staphylococcus aureus. ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761997000500013

    authors: Lambertucci JR,Rayes AA,Barata CH,Teixeira R,Gerspacher-Lara R

    更新日期:1997-09-01 00:00:00

  • [Action of a molluscacide (Bayluscide-SRB) from the Dynatech R/D Company in lakes of northeastern Brazil--Sergipe, Brazil].

    abstract::To study the action of molluscicide nine ponds were selected: 3 of them lying in Maruim municipality, 29 km far from north Aracaju, the State capital, and 6 ponds in Itabaianinha municipality, 118 km far from south Aracaju. This study was carried out for 16 months. Environmental parameters observed were those thought ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761987000800061

    authors: Rosas E,Ribeiro EC

    更新日期:1987-01-01 00:00:00

  • [Epidemiology of human leptospirosis, with special reference to greater Rio de Janeiro, Brazil, from 1970 to 1982].

    abstract::From 1970 to 1982, 884 cases of human leptospirosis were serologically diagnosed at the Leptospirosis Laboratory, Fundação Oswaldo Cruz - FIOCRUZ, by means of the microscopic agglutination test. Of these patients, 775 were hospitalized in the city of Rio de Janeiro, and 109 were reported by other Brazilian cities. Ict...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761987000100016

    authors: Andrade J,Brandão AP

    更新日期:1987-01-01 00:00:00

  • In vitro synergic effect of beta-lapachone and isoniazid on the growth of Mycobacterium fortuitum and Mycobacterium smegmatis.

    abstract::Nontuberculous mycobacteria are ubiquitous and saprophytic organisms that have been implicated in a wide spectrum of diseases due to an increasing number of immunocompromised patients. The natural resistance of atypical mycobacteria to classical antituberculous drugs has encouraged research into new chemotherapeutic a...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762009000400008

    authors: Silva JL,Mesquita AR,Ximenes EA

    更新日期:2009-07-01 00:00:00

  • The control of schistosomiasis: epidemiological aspects of reinfection.

    abstract::Current strategies for schistosomiasis control rely heavily on drug treatment intended to reduce morbidity and disease to an insignificant level of public health importance. This approach reduces but rarely, if ever, eliminates transmission. Indeed, snail infection rates may be little altered by such chemotherapy. Rei...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02761989000500014

    authors: Sturrock RF

    更新日期:1989-10-01 00:00:00

  • Correlations between major risk factors and closely related Mycobacterium tuberculosis isolates grouped by three current genotyping procedures: a population-based study in northeast Mexico.

    abstract::The characteristics of tuberculosis (TB) patients related to a chain of recent TB transmissions were investigated. Mycobacterium tuberculosis (MTB) isolates (120) were genotyped using the restriction fragment length polymorphism-IS6110 (R), spacer oligotyping (S) and mycobacterial interspersed repetitive units-variabl...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276130550

    authors: Peñuelas-Urquides K,Martínez-Rodríguez HG,Enciso-Moreno JA,Molina-Salinas GM,Silva-Ramírez B,Padilla-Rivas GR,Vera-Cabrera L,Torres-de-la-Cruz VM,Martínez-Martínez YB,Ortega-García JL,Garza-Treviño EN,Enciso-Moreno L,Saucedo-Cárd

    更新日期:2014-09-01 00:00:00

  • Applications of flow cytometry to hematopoietic stem cell transplantation.

    abstract::Applications of flow cytometry to clinical and experimental hematopoietic stem cell transplantation (HSCT) are discussed in this review covering the following topics: diagnosis and classification of lymphohematologic disorders, quantitation of hematopoietic progenitors in the graft, lymphohematopoietic reconstitution ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02762000000300018

    authors: Voltarelli JC

    更新日期:2000-05-01 00:00:00

  • Distribution of phlebotomine fauna (Diptera: Psychodidae) across an urban-rural gradient in an area of endemic visceral leishmaniasis in northern Brazil.

    abstract::The number of visceral leishmaniasis (VL) cases has increased over the past 10 years in Brazil, especially in the North and Northeast regions of the country. The aim of this study was to evaluate the urbanisation of VL vectors in Barcarena, Pará, an area in northern Brazil where VL is endemic. Sandflies were captured ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762011000800023

    authors: Oliveira DM,Saraiva EM,Ishikawa EA,Sousa AA,Silva EO,Silva IM

    更新日期:2011-12-01 00:00:00

  • A comparative evaluation of end-emic and non-endemic region of visceral leishmaniasis (Kala-azar) in India with ground survey and space technology.

    abstract::In visceral leishmaniasis, phlebotomine vectors are targets for control measures. Understanding the ecosystem of the vectors is a prerequisite for creating these control measures. This study endeavours to delineate the suitable locations of Phlebotomus argentipes with relation to environmental characteristics between ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762011000500001

    authors: Kesari S,Bhunia GS,Kumar V,Jeyaram A,Ranjan A,Das P

    更新日期:2011-08-01 00:00:00

  • Sequencing and identification of expressed Schistosoma mansoni genes by random selection of cDNA clones from a directional library.

    abstract::We have initiated a gene discovery program in Schistosoma mansoni based on the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of cDNAs obtained from single passes in automatic DNA sequences. ESTs can be used to identify genes on the basis of their homology with sequences from other species deposit...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761995000200015

    authors: Franco GR,Simpson AJ,Pena SD

    更新日期:1995-03-01 00:00:00

  • From allergy to schistosomes: role of Fc receptors and adhesion molecules in eosinophil effector function.

    abstract::The dual function of eosinophils has been evidenced in protective immunity against parasites as well as in pathological manifestations during allergic disorders. We have demonstrated that a new class of IgE receptors, Fc epsilon RII/CD23, was involved in the functional duality of eosinophils and other proinflammatory ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02761997000800003

    authors: Nutten S,Trottein F,Gounni AS,Papin JP,Capron A,Capron M

    更新日期:1997-01-01 00:00:00

  • Uptake of macromolecules by cercariae during skin penetration and transformation to schistosomula (Schistosoma mansoni).

    abstract::Here, we observed the uptake of membrane-impermeant molecules by cercariae as they penetrate the skin and are transformed into schistosomula. We propose that membrane-impermeant molecules, Lucifer Yellow, Propidium iodide and Hoechst 33258 enter the parasite through both thenephridiopore and the surface membrane and t...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762010000400007

    authors: Thornhill J,Kusel J,Oliviera FA,Ribeiro F,Lima SF,Coelho PM,McVeigh P,Mattos AC

    更新日期:2010-07-01 00:00:00

  • Life cycle, feeding and defecation patterns of Rhodnius ecuadoriensis (Lent & León 1958) (Hemiptera: Reduviidae: Triatominae) under laboratory conditions.

    abstract::Rhodnius ecuadoriensis is the second most important vector of Chagas Disease (CD) in Ecuador. The objective of this study was to describe (and compare) the life cycle, the feeding and defecation patterns under laboratory conditions of two populations of this specie [from the provinces of Manabí (Coastal region) and Lo...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762008000700011

    authors: Villacís AG,Arcos-Terán L,Grijalva MJ

    更新日期:2008-11-01 00:00:00

  • Early Cretaceous trypanosomatids associated with fossil sand fly larvae in Burmese amber.

    abstract::Early Cretaceous flagellates with characters typical of trypanosomatids were found in the gut of sand fly larvae, as well as in surrounding debris, in Burmese amber. This discovery supports a hypothesis in which free-living trypanosomatids could have been acquired by sand fly larvae in their feeding environment and th...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762007005000070

    authors: Poinar G Jr

    更新日期:2007-08-01 00:00:00

  • Differentiation and genetic analysis of Rhodnius prolixus and Rhodnius colombiensis by rDNA and RAPD amplification.

    abstract::Domiciliated Rhodnius prolixus and sylvatic R. colombiensis were analyzed in order to confirm their genetic divergence and verify the risk that the latter represents in the domiciliation process, and to provide tools for identifying the sources of possible reinfestation by triatomines in human dwellings allowing contr...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762001000800003

    authors: Jaramillo C,Montaña MF,Castro LR,Vallejo GA,Guhl F

    更新日期:2001-11-01 00:00:00

  • Comparative descriptions of eggs from three species of Rhodnius (Hemiptera: Reduviidae: Triatominae).

    abstract::The authors describe and compare the morphological and ultrastructural characteristics of eggs from the three most recent described species of the genus Rhodnius Stål, 1859, which have not previously been studied. These species are Rhodnius colombiensis (Mejia, Galvão & Jurberg 1999), Rhodnius milesi (Carcavallo, Roch...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762009000700013

    authors: Santos CM,Jurberg J,Galvão C,Rosa JA,Júnior WC,Barata JM,Obara MT

    更新日期:2009-11-01 00:00:00

  • FLI1 gene influences lesion size and skin test may predict therapeutic response in cutaneous leishmaniasis.

    abstract::Genes associated with wound healing have been shown to be risk factors for cutaneous leishmaniasis (CL) which is caused by Leishmania braziliensis. In this study, we examined whether the genes previously associated with CL influenced the clinical outcome. Patients were genotyped and retrospectively classified as respo...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760190361

    authors: da Hora AS,de Almeida LF,do Lago TS,Machado PR,Castellucci LC

    更新日期:2020-03-02 00:00:00

  • Present aspects of immunodiagnosis of schistosomiasis.

    abstract::Facilitated and improved by advances in molecular biology, techniques for the immunodiagnosis of schistosomiasis, including assays based on the detection of antigens circulating in the serum and/or excreted in the urine, have now reached the stage of multi-centre trials. There is a need to complement parasitological t...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02761992000800004

    authors: Bergquist NR

    更新日期:1992-01-01 00:00:00

  • Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells.

    abstract::Acute infection with Trypanosoma cruzi results in intense myocarditis, which progresses to a chronic, asymptomatic indeterminate form. The evolution toward this chronic cardiac form occurs in approximately 30% of all cases of T. cruzi infection. Suppression of delayed type hypersensitivity (DTH) has been proposed as a...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-0276108062013004

    authors: Thé TS,Portella RS,Guerreiro ML,Andrade SG

    更新日期:2013-09-01 00:00:00

  • Symbiont modulates expression of specific gene categories in Angomonas deanei.

    abstract::Trypanosomatids are parasites that cause disease in humans, animals, and plants. Most are non-pathogenic and some harbor a symbiotic bacterium. Endosymbiosis is part of the evolutionary process of vital cell functions such as respiration and photosynthesis. Angomonas deanei is an example of a symbiont-containing trypa...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160228

    authors: Penha LL,Hoffmann L,Souza SS,Martins AC,Bottaro T,Prosdocimi F,Faffe DS,Motta MC,Ürményi TP,Silva R

    更新日期:2016-11-01 00:00:00

  • Effects of gamma irradiation on the reproductive capacity and the longevity of Panstrongylus megistus (Hemiptera: Reduviidae).

    abstract::The effects of radiation on the reproductive capacity and the longevity of Panstrongylus megistus were studied. An indirect correlation between longevity and radiation doses was observed. Males were more affected than females. Longevity of females submitted to 10 Gy was not different when compared to controls. Some of...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761993000200004

    authors: Verçosa P,Falcão PH,Furtado AF

    更新日期:1993-04-01 00:00:00

  • Phylogeny and evolution of the aspartyl protease family from clinically relevant Candida species.

    abstract::Aspartyl proteases are a class of enzymes that include the yeast aspartyl proteases and secreted aspartyl protease (Sap) superfamilies. Several Sap superfamily members have been demonstrated or suggested as virulence factors in opportunistic pathogens of the genus Candida. Candida albicans, Candida tropicalis, Candida...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762009000300018

    authors: Parra-Ortega B,Cruz-Torres H,Villa-Tanaca L,Hernández-Rodríguez C

    更新日期:2009-05-01 00:00:00

  • Ecogenetics of Triatoma sordida and Triatoma guasayana (Hemiptera: reduviidae) in the Bolivian chaco.

    abstract::Triatoma guasayana and two putative cryptic species pertaining to T. sordida complex (named groups 1 and 2) occur in sympatry in the Bolivian Chaco. Using multilocus enzyme electrophoresis and subsequent genetic analysis, our work assesses their population distribution and dispersal capacity in domestic, peridomestic,...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/S0074-02761999000400004

    authors: Noireau F,Gutierrez T,Flores R,Breniere F,Bosseno MF,Wisnivesky-Colli C

    更新日期:1999-07-01 00:00:00