Fungal-host interactions: insights into microRNA in response to Paracoccidioides species.

Abstract:

:BACKGROUND Paracoccidioides spp. causes paracoccidioidomycosis (PCM), an important and frequent systemic mycosis that occurs in Latin America. The infectious process begins with contact between the fungus and lung cells, and the molecular pattern of this interaction is currently poorly understood. MicroRNAs (miRNAs) are small non-coding RNAs that regulate the gene expression in many biological processes, including in the infections. OBJECTIVE This study aimed to analyse the expression of miRNAs in lung cells as response to infection by Paracoccidioides spp. METHODS A quantitative real-time polymerase chain reaction (RT-qPCR) based screening was employed to verify differentially expressed miRNAs in human lung cells infected with three different species; Paracoccidioides lutzii, Paracoccidioides americana, and Paracoccidioides brasiliensis. Furthermore, the in silico predictions of target genes and pathways for miRNAs were obtained. FINDINGS The results showed that miRNAs identified in the lung cells were different according to the species studied. However, based on the predicted targets, the potential signaling pathways regulated by miRNAs are common and related to adhesion, actin cytoskeleton rearrangement, apoptosis, and immune response mediated by T cells and TGF-β. MAIN CONCLUSIONS In summary, this study showed the miRNAs pattern of epithelial cells in response to infection by Paracoccidioides species and the potential role of these molecules in the regulation of key pathogenesis mechanisms of PCM.

journal_name

Mem Inst Oswaldo Cruz

authors

Singulani JL,Silva JFD,Gullo FP,Costa MC,Fusco-Almeida AM,Enguita FJ,Mendes-Giannini MJS

doi

10.1590/0074-02760200238

subject

Has Abstract

pub_date

2020-01-01 00:00:00

pages

e200238

eissn

0074-0276

issn

1678-8060

pii

S0074-02762020000100334

journal_volume

115

pub_type

杂志文章
  • The rational search for natural neolignans.

    abstract::A rational method of search for natural neolignans of desired structures is outlined. This involves consultation of a collection of chemical profiles of plant families. The profiles are assembled considering the biosynthetic class (in the present case lignoids), subclass (neolignans), structural types (neolignan skele...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/s0074-02761991000600009

    authors: Gottlieb OR

    更新日期:1991-01-01 00:00:00

  • Citral and carvone chemotypes from the essential oils of Colombian Lippia alba (Mill.) N.E. Brown: composition, cytotoxicity and antifungal activity.

    abstract::Two essential oils of Lippia alba (Mill.) N.E. Brown (Verbenacea), the carvone and citral chemotypes and 15 of their compounds were evaluated to determine cytotoxicity and antifungal activity. Cytotoxicity assays for both the citral and carvone chemotypes were carried out with tetrazolium-dye, which showed a dose-depe...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762009000600010

    authors: Mesa-Arango AC,Montiel-Ramos J,Zapata B,Durán C,Betancur-Galvis L,Stashenko E

    更新日期:2009-09-01 00:00:00

  • Isolation and identification of 9-methylgermacrene-B as the putative sex pheromone of Lutzomyia cruzi (Mangabeira, 1938) (Diptera: Psychodidae).

    abstract::Lutzomyia (Lutzomyia) cruzi has been named as a probable vector of Leishmania chagasi in Corumbá, Mato Grosso do Sul, Brazil. Taxonomically L. cruzi is closely related to the L. longipalpis species complex. Females of L. cruzi and L. longipalpis are morphologically indistinguishable and associated males must be examin...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000300030

    authors: Brazil RP,Hamilton JG

    更新日期:2002-04-01 00:00:00

  • Aspects of classification of Hemiptera hemocytes from six triatomine species.

    abstract::The objective of this work was to characterize, and compare different morphological types of hemocytes of Rhodnius prolixus, Rhodnius robustus, Rhodnius neglectus, Triatoma infestans, Panstrongylus megistus, and Dipetalogaster maximus. This information provides the basis for studying the cellular immune systems of the...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761991000100002

    authors: de Azambuja P,Garcia ES,Ratcliffe NA

    更新日期:1991-01-01 00:00:00

  • Rhabdias paraensis sp. nov.: a parasite of the lungs of Rhinella marina (Amphibia: Bufonidae) from Brazilian Amazonia.

    abstract::The nematode parasites of Rhinella marina include species of the genus Rhabdias (Rhabdiasidae: Rhabditoidea). The present study describes Rhabdias paraensis sp. nov., which parasitizes the lungs of R. marina in Brazilian Amazonia. Of the more than 70 known species of this genus, 18 are parasites of bufonids, of which,...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762011000400008

    authors: Santos JN,Melo FT,Nascimento Lde C,Nascimento DE,Giese EG,Furtado AP

    更新日期:2011-06-01 00:00:00

  • Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil.

    abstract::An epidemic of sporotrichosis, a subcutaneous mycosis caused by the fungus Sporothrix schenckii, is ongoing in Rio de Janeiro, Brazil, in which cases of human infection are related to exposure to cats. In an attempt to demonstrate the zoonotic character of this epidemic using molecular methodology, we characterised by...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762009000500018

    authors: Reis RS,Almeida-Paes R,Muniz Mde M,Tavares PM,Monteiro PC,Schubach TM,Gutierrez-Galhardo MC,Zancopé-Oliveira RM

    更新日期:2009-08-01 00:00:00

  • Mansonella ozzardi in Brazil: prevalence of infection in riverine communities in the Purus region, in the state of Amazonas.

    abstract::This study was undertaken to investigate the prevalence of Mansonella ozzardi infection and to estimate the parasitic infection rate (PIR) in simuliid black flies in the municipality of Pauini, Amazonas, Brazil. We used thick blood films to examine 921 individuals in 35 riverine communities along the Pauini and Purus ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762009000100012

    authors: Medeiros JF,Py-Daniel V,Barbosa UC,Izzo TJ

    更新日期:2009-02-01 00:00:00

  • Evaluation of spleen cell population and effect of splenectomy on granuloma modulation in BALB/c mice infected with Schistosoma mansoni.

    abstract::A kinetic study of the cells present in the spleen of BALB/c mice infected with Schistosoma mansoni was carried out. The lymphocytes were evaluated phenotypically with monoclonal antibodies and the effect of splenectomy on the modulation of periovular granuloma was also investigated. The infected mice had proportional...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761993000100015

    authors: Arruda SM,Santoro F,Sadigursky M

    更新日期:1993-01-01 00:00:00

  • Triatoma jatai sp. nov. in the state of Tocantins, Brazil (Hemiptera: Reduviidae: Triatominae).

    abstract::Triatoma jatai sp. nov. is the first new species of triatomine to be described in the state of Tocantins, in the northern region of Brazil. It was caught on rock outcrops in the wild environment and, more recently, invading homes. While T. jatai sp. nov. is morphologically similar to Triatoma costalimai, it is disting...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/S0074-0276108042013006

    authors: Gonçalves TC,Teves-Neves SC,Santos-Mallet JR,Carbajal-de-la-Fuente AL,Lopes CM

    更新日期:2013-06-01 00:00:00

  • Surveillance of antiviral resistance markers in Argentina: detection of E119V neuraminidase mutation in a post-treatment immunocompromised patient.

    abstract::Although vaccines are the best means of protection against influenza, neuraminidase inhibitors are currently the main antiviral treatment available to control severe influenza cases. One of the most frequent substitutions in the neuraminidase (NA) protein of influenza A(H3N2) viruses during or soon after oseltamivir a...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160262

    authors: Pontoriero A,Avaro M,Benedetti E,Russo M,Czech A,Periolo N,Campos A,Zamora A,Baumeister E

    更新日期:2016-12-01 00:00:00

  • Field evaluation of effectiveness of the BG-Sentinel, a new trap for capturing adult Aedes aegypti (Diptera: Culicidae).

    abstract::In recent years, the development of new tools to gather field information about vector ecological parameters has increased. This report evaluated the BG-Sentinel Trap (BGS-Trap), a promising new attempt to improve collection of the dengue vector, Aedes aegypti. The efficacy of the BGS-Trap was compared with the CDC ba...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762006000300017

    authors: Maciel-de-Freitas R,Eiras AE,Lourenço-de-Oliveira R

    更新日期:2006-05-01 00:00:00

  • Isolation and identification of actin-binding proteins in Plasmodium falciparum by affinity chromatography.

    abstract::The invasion of the erythrocyte by Plasmodium falciparum depends on the ability of the merozoite to move through the membrane invagination. This ability is probably mediated by actin dependent motors. Using affinity columns with G-actin and F-actin we isolated actin binding proteins from the parasite. By immunoblottin...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762000000300007

    authors: Forero C,Wasserman M

    更新日期:2000-05-01 00:00:00

  • In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis.

    abstract::Mutations in the katG gene have been identified and correlated with isoniazid (INH) resistance in Mycobacterium tuberculosis isolates. The mutation AGC-->ACC (Ser-->Thr) at katG315 has been reported to be the most frequent and is associated with transmission and multidrug resistance. Rapid detection of this mutation c...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762009000500008

    authors: Verza M,Maschmann Rde A,Silva MS,Dalla Costa ER,Ribeiro MO,Rosso F,Suffys PN,Tortoli E,Marcelli F,Zaha A,Rossetti ML

    更新日期:2009-08-01 00:00:00

  • Acoustic communication in insect disease vectors.

    abstract::Acoustic signalling has been extensively studied in insect species, which has led to a better understanding of sexual communication, sexual selection and modes of speciation. The significance of acoustic signals for a blood-sucking insect was first reported in the XIX century by Christopher Johnston, studying the hear...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章,评审

    doi:10.1590/0074-0276130390

    authors: Vigoder Fde M,Ritchie MG,Gibson G,Peixoto AA

    更新日期:2013-01-01 00:00:00

  • Characterisation of ocular involvement in an experimental model of neuroschistosomiasis mansoni.

    abstract::The Global Burden of Disease Study 2010 listed schistosomiasis among the leading 100 causes of death in Brazil, responsible for 3.6% of the estimated total of deaths globally. Eye and adnexa are very rarely affected by schistosomiasis mansoni, with limited documentation of ocular pathology in this setting. This short ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760190029

    authors: Fidelis TAA,Brasileiro-Filho G,Santos HH,Vasconcelos-Santos DV,Parreiras PM,Coelho PMZ,Araujo N,Chaud MV,Lambertucci JR

    更新日期:2019-01-01 00:00:00

  • Chagas disease: national survey of seroprevalence in children under five years of age conducted in 2008.

    abstract:BACKGROUND:Since the early 1990s, programs to control Chagas disease in South America have focused on eradicating domiciliary Triatoma infestans, the main vector. Seroprevalence studies of the chagasic infection are included as part of the vector control programs; they are essential to assess the impact of vector contr...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760160407

    authors: Russomando G,Cousiño B,Sanchez Z,Franco LX,Nara EM,Chena L,Martínez M,Galeano ME,Benitez L

    更新日期:2017-05-01 00:00:00

  • A new species of Thelastomathidae (Nematoda) a parasite of Neocurtilla claraziana Saussure (Orthoptera, Gryllotalpidae) in Argentina.

    abstract::Gryllophila cephalobulata n. sp. (Nematoda, Thelastomatidae) a parasite of the mole cricket Neocurtilla claraziana (Orthoptera, Gryllotalpidae) isolated in Buenos Aires Province, is described and illustrated. It is characterized by cuticle annulated all along the length of the body; the first ring has 4 lobules, the s...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762002000500012

    authors: Camino NB,Maiztegui B

    更新日期:2002-07-01 00:00:00

  • Detection and characterization of rotavirus G and P types from children participating in a rotavirus vaccine trial in Belém, Brazil.

    abstract::This study sought the characterization of rotaviruses in a trial with a tetravalent rhesus-human rotavirus vaccine in Belém, Brazil in children who received three doses of vaccine or placebo in the 1st, 3rd and 5th months of life. Rotavirus electropherotypes, subgroups, G serotypes, G, [P] and [P], G genotypes were de...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1590/s0074-02762002000100020

    authors: Mascarenhas JD,Linhares AC,Gabbay YB,Leite JP

    更新日期:2002-01-01 00:00:00

  • New host and locality records for Tetrameres (Gynaecophila) spirospiculum Pinto & Vicente, 1995 (Nematoda: Tetrameridae), with new morphological data.

    abstract::We report the finding of Tetrameres spirospiculum Pinto & Vicente, 1995 from Theristicus melanopis melanopis (Threskiornithidae) from Patagonia, Argentina. These constitute new host and locality records. We propose the assignation of this species to the subgenus T. (Gynaecophila) Gubanov, 1950, based on the presence o...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762001000300009

    authors: Digiani MC,Cremonte F

    更新日期:2001-04-01 00:00:00

  • Monitoring the circulation of rotavirus among children after the introduction of the Rotarix™ vaccine in Goiânia, Brazil.

    abstract::The epidemiological features of rotavirus A (RVA) infection differ between children from developing and developed countries which could result in differences in vaccine efficacy around the world. To evaluate the impact of Rotarix™ on RVA prevalence, we monitored RVA genotypes circulating in Goiânia by monitoring virus...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762011000400018

    authors: Borges AM,Dias e Souza M,Fiaccadori FS,Cardoso Dd

    更新日期:2011-06-01 00:00:00

  • More productive in vitro culture of Cryptosporidium parvum for better study of the intra- and extracellular phases.

    abstract::The great difficulties in treating people and animals suffering from cryptosporidiosis have prompted the development of in vitro experimental models. Due to the models of in vitro culture, new extracellular stages of Cryptosporidium have been demonstrated. The development of these extracellular phases depends on the t...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762007005000071

    authors: Perez Cordón G,Marin C,Romero D,Rosales C,Sánchez Moreno M,Rosales MJ

    更新日期:2007-08-01 00:00:00

  • A chromosome 9 deletion in Plasmodium falciparum results in loss of cytoadherence.

    abstract::Many lines of Plasmodium falciparum undergo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and the selected cells produce gametocy...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761992000700011

    authors: Kemp DJ,Thompson J,Barnes DA,Triglia T,Karamalis F,Petersen C,Brown GV,Day KP

    更新日期:1992-01-01 00:00:00

  • New insights into trypanosomatid U5 small nuclear ribonucleoproteins.

    abstract::Several protozoan parasites exist in the Trypanosomatidae family, including various agents of human diseases. Multiple lines of evidence suggest that important differences are present between the translational and mRNA processing (trans splicing) systems of trypanosomatids and other eukaryotes. In this context, certai...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762011000200003

    authors: Silva MT,Ambrósio DL,Trevelin CC,Watanabe TF,Laure HJ,Greene LJ,Rosa JC,Valentini SR,Cicarelli RM

    更新日期:2011-03-01 00:00:00

  • Human toxocariasis: a seroepidemiological survey in schoolchildren of Sorocaba, Brazil.

    abstract::A seroepidemiological survey for toxocariasis, among 180 schoolchildren of the public schools of Sorocaba City, state of São Paulo, Brazil, was carried out from August 2000 to July 2001. ELISA test was performed using excretory and secretory antigens for the detection of IgG anti-Toxocara antibodies. Information regar...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:/S0074-02762004000600002

    authors: Coelho LM,Silva MV,Dini CY,Giacon Neto AA,Novo NF,Silveira EP

    更新日期:2004-10-01 00:00:00

  • Synonymy between Lymnaea bogotensis Pilsbry, 1935 and Lymnaea cousini Jousseaume, 1887 (Gastropoda: Lymnaeidae).

    abstract::The lymneid snail Lymnaea bogotensis Pilsbry 1935 is synonymized with L. cousini Jousseaume 1887, based on morphological comparisons of the reproductive systems. The shell, renal tube, and reproductive system are described and illustrated from specimens collected in the type locality and the municipality of Paipa, Col...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762006000700015

    authors: Velásquez LE

    更新日期:2006-11-01 00:00:00

  • Acute experimental Trypanosoma cruzi infection: establishing a murine model that utilises non-invasive measurements of disease parameters.

    abstract::Trypanosoma cruzi infection has a large public health impact in Latin American countries. Although the transmission rates via blood transfusions and insect vectors have declined sharply in the past 20 years due to policies of the Southern Cone countries, a large number of people are still at risk for infection. Curren...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762012000200010

    authors: Silva DR,Castro SL,Alves MC,Batista Wda S,Oliveira GM

    更新日期:2012-03-01 00:00:00

  • Feeding and defaecation patterns in Triatoma sordida.

    abstract::Triatoma sordida is a peridomestic Triatominae that could play an important role in the transmission of Trypanosoma cruzi, although its vectorial competence is not well known. The aim of this work was to evaluate two aspects of the vectorial competence: the feeding behaviour and defaecation patterns, and to compare th...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02761996000400004

    authors: Crocco LB,Catalá SS

    更新日期:1996-07-01 00:00:00

  • Deep sequencing of small RNAs reveals the repertoire of miRNAs and piRNAs in Biomphalaria glabrata.

    abstract:BACKGROUND:Biomphalaria glabrata snails are widely distributed in schistosomiasis endemic areas like America and Caribe, displaying high susceptibility to infection by Schistosoma mansoni. After the availability of B. glabrata genome and transcriptome data, studies focusing on genetic markers and small non-coding RNAs ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/0074-02760190498

    authors: Queiroz FR,Portilho LG,Jeremias WJ,Babá ÉH,do Amaral LR,Silva LM,Coelho PMZ,Caldeira RL,Gomes MS

    更新日期:2020-07-01 00:00:00

  • Crossing experiments detect genetic incompatibility among populations of Triatoma brasiliensis Neiva, 1911 (Heteroptera, Reduviidae, Triatominae).

    abstract::Triatoma brasiliensis is composed of at least four geographic populations (brasiliensis, melanica, macromelasoma, and juazeiro) that have distinct chromatic, morphologic, biologic and ecologic patterns, and genetic composition. Reciprocal crosses between all pairwise combinations were carried out in order to evaluate ...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762003000500009

    authors: Costa J,Almeida CE,Dujardin JP,Beard CB

    更新日期:2003-07-01 00:00:00

  • Malpighian tubule polytene chromosomes of Culex quinquefasciatus (Diptera, Culicinae).

    abstract::Dipteran polytene chromosomes provide an excellent model for understanding in species complexes, as well as for structural and functional cytogenetics. The status of species in the Culex pipiens complex is controversial and the use of polytene chromosomes for cytogenetic analysis in the subfamily Culicinae has been di...

    journal_title:Memorias do Instituto Oswaldo Cruz

    pub_type: 杂志文章

    doi:10.1590/s0074-02762003000300016

    authors: Campos J,Andrade CF,Recco-Pimentel SM

    更新日期:2003-04-01 00:00:00