Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages.

Abstract:

:Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was decreased in knockout and complemented strains. The cdd disruption does not affect M. tuberculosis growth both in Mid- dlebrook 7H9 and in RAW 264.7 cells, which indicates that cdd is not important for macrophage invasion and virulence.

journal_name

Mem Inst Oswaldo Cruz

authors

Villela AD,Rodrigues-Junior VS,Pinto AFM,Falcão VCA,Sánchez-Quitian ZA,Eichler P,Bizarro CV,Basso LA,Santos DS

doi

10.1590/0074-02760170105

subject

Has Abstract

pub_date

2017-11-01 00:00:00

pages

785-789

issue

11

eissn

0074-0276

issn

1678-8060

pii

S0074-02762017001100785

journal_volume

112

pub_type

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