The use of polyvinyl alcohol glutaraldehyde as solid-phase in ELISA for plague.

Abstract:

:Discs of polyvinyl alcohol cross-linked with glutaraldehyde were synthesized under acid catalysis (H2SO4). Then, the antigen F1 purified from Yersinia pestis was covalently linked to this modified polymer. Afterwards, an enzyme-linked immunosorbent assay (ELISA) was established for the diagnosis of plague in rabbit and human. The best conditions for the method were achieved by using 1.3 micrograms of F1 prepared in 0.067 M phosphate buffer, pH 7.2, containing 1 M NaCl (PBS); anti-IgG peroxidase conjugate diluted 6,000 times and as a blocking agent 3% w/v skim milk in PBS. The titration of positive rabbit serum according to this procedure detected antibody concentrations up to 1:12,800 times. The present method, the conventional ELISA and passive haemagglutination assay are compared.

journal_name

Mem Inst Oswaldo Cruz

authors

Araujo AM,Petribú AT,Sales Barbosa GH,Diniz JR,de Almeida AM,Mendes Azevedo W,Malagueño E,Carvalho Júnior LB

doi

10.1590/s0074-02761996000200014

subject

Has Abstract

pub_date

1996-03-01 00:00:00

pages

195-8

issue

2

eissn

0074-0276

issn

1678-8060

pii

S0074-02761996000200014

journal_volume

91

pub_type

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