First isolation of West Nile virus in Brazil.

Abstract:

BACKGROUND:Serological evidence of West Nile virus (WNV) infection has been reported in different regions of Brazil from equine and human hosts but the virus had never been isolated in the country. OBJECTIVES:We sought to identify the viral etiology of equine encephalitis in Espírito Santo state. METHODS:We performed viral culture in C6/36 cells, molecular detection of WNV genome, histopathology and immunohistochemistry from horse cerebral tissue. We also carried out sequencing, phylogenetic analysis and molecular clock. FINDINGS:Histopathologic analysis from horse cerebral tissue showed injury related to encephalitis and WNV infection was confirmed by immunohistochemistry. The virus was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) from brain tissue and subsequently isolated in C6/36 cells. WNV full-length genome was sequenced showing the isolated strain belongs to lineage 1a. The molecular clock indicated that Brazilian WNV strain share the same common ancestor that were circulating in US during 2002-2005. MAIN CONCLUSIONS:Here we report the first isolation of WNV in Brazil from a horse with neurologic disease, which was clustered into lineage 1a with others US WNV strains isolated in beginning of 2000's decade.

journal_name

Mem Inst Oswaldo Cruz

authors

Martins LC,Silva EVPD,Casseb LMN,Silva SPD,Cruz ACR,Pantoja JAS,Medeiros DBA,Martins Filho AJ,Cruz EDRMD,Araújo MTF,Cardoso JF,Cunha MACRD,Almada GL,Romano APM,Santos MGDP,Rodrigues GAP,Chiang JO,Quaresma JAS,Carvalho

doi

10.1590/0074-02760180332

subject

Has Abstract

pub_date

2019-01-17 00:00:00

pages

e180332

eissn

0074-0276

issn

1678-8060

pii

S0074-02762019000100305

journal_volume

114

pub_type

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