Abstract:
BACKGROUND:Serological evidence of West Nile virus (WNV) infection has been reported in different regions of Brazil from equine and human hosts but the virus had never been isolated in the country. OBJECTIVES:We sought to identify the viral etiology of equine encephalitis in Espírito Santo state. METHODS:We performed viral culture in C6/36 cells, molecular detection of WNV genome, histopathology and immunohistochemistry from horse cerebral tissue. We also carried out sequencing, phylogenetic analysis and molecular clock. FINDINGS:Histopathologic analysis from horse cerebral tissue showed injury related to encephalitis and WNV infection was confirmed by immunohistochemistry. The virus was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) from brain tissue and subsequently isolated in C6/36 cells. WNV full-length genome was sequenced showing the isolated strain belongs to lineage 1a. The molecular clock indicated that Brazilian WNV strain share the same common ancestor that were circulating in US during 2002-2005. MAIN CONCLUSIONS:Here we report the first isolation of WNV in Brazil from a horse with neurologic disease, which was clustered into lineage 1a with others US WNV strains isolated in beginning of 2000's decade.
journal_name
Mem Inst Oswaldo Cruzjournal_title
Memorias do Instituto Oswaldo Cruzauthors
Martins LC,Silva EVPD,Casseb LMN,Silva SPD,Cruz ACR,Pantoja JAS,Medeiros DBA,Martins Filho AJ,Cruz EDRMD,Araújo MTF,Cardoso JF,Cunha MACRD,Almada GL,Romano APM,Santos MGDP,Rodrigues GAP,Chiang JO,Quaresma JAS,Carvalhodoi
10.1590/0074-02760180332subject
Has Abstractpub_date
2019-01-17 00:00:00pages
e180332eissn
0074-0276issn
1678-8060pii
S0074-02762019000100305journal_volume
114pub_type
杂志文章abstract::Genomic DNA fragments from males of Psychodopygus wellcomei were isolated and shown to be useful as sensitive diagnostic probes for positively separating individuals of this species from those of Ps. complexus. These two members of the Ps. squamiventris series are found sympatrically in foci of cutaneous leishmaniasis...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761991000100008
更新日期:1991-01-01 00:00:00
abstract::The characteristics of tuberculosis (TB) patients related to a chain of recent TB transmissions were investigated. Mycobacterium tuberculosis (MTB) isolates (120) were genotyped using the restriction fragment length polymorphism-IS6110 (R), spacer oligotyping (S) and mycobacterial interspersed repetitive units-variabl...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-0276130550
更新日期:2014-09-01 00:00:00
abstract::The number of visceral leishmaniasis (VL) cases has increased over the past 10 years in Brazil, especially in the North and Northeast regions of the country. The aim of this study was to evaluate the urbanisation of VL vectors in Barcarena, Pará, an area in northern Brazil where VL is endemic. Sandflies were captured ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762011000800023
更新日期:2011-12-01 00:00:00
abstract::This paper presents epidemiological, laboratory, and clinical data on 12 years of dengue virus activity in the State of Rio de Janeiro from the time the disease was first confirmed virologically in April 1986 through April 1998. DEN-1 and DEN-2 viruses are the serotypes circulating in the state and were responsible fo...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761999000300004
更新日期:1999-05-01 00:00:00
abstract::There is no clear understanding of the outcome of reinfection in New World cutaneous leishmaniasis, and its role in the relationship to the development of protection or secondary disease. For this reason, reinfection experiments with homologous (Leishmania panamensis-L. panamensis) and heterologous (L. major-L. paname...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761998000300015
更新日期:1998-05-01 00:00:00
abstract::The Brazilian variant of human immunodeficiency virus type 1 (HIV-1) subtype B, (serotype B"-GWGR), has a tryptophan replacing the proline in position 328 the HIV-1 envelope. A longer median time period from infection to acquired immunodeficiency syndrome (AIDS) for serotype B (B"-GWGR) infected subjects compared to t...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762004000100012
更新日期:2004-02-01 00:00:00
abstract::The vaccine Bacillus of Calmette Guérin (BCG) was originally developed in France as an oral vaccine against tuberculosis. The oral use of this vaccine was replaced by the parenteral route in almost all countries after the Lubeck disaster. In contrast, Brazil retained the oral delivery of the vaccine until the mid-seve...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 历史文章,杂志文章,评审
doi:10.1590/s0074-02762005000500002
更新日期:2005-08-01 00:00:00
abstract::The evolutionary relationships of sand flies and Leishmania are discussed in this report, which draws distinctions between co-association, co-evolution and co-speciation (or co-cladogenesis). Examples focus on Phlebotomus vectors of Le. infantum and Le. major in the Mediterranean subregion. ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762000000400024
更新日期:2000-07-01 00:00:00
abstract::Studies on the sandfly fauna to evaluate natural infection indexes are still limited in the Brazilian Amazon, a region with an increasing incidence of cutaneous leishmaniasis. Here, by using a multiplex polymerase chain reaction directed to Leishmania kDNA and hybridisation, we were able to identify L. (Viannia) subge...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-02760160283
更新日期:2017-01-01 00:00:00
abstract::The seroprevalence of toxoplasmosis in 832 pregnant women in Miracema, Rio de Janeiro, was determined and 75.1% (625) and 2.0% (17) were anti-Toxoplasma gondii IgG and IgM positive, respectively. Out of the 17 IgM positive pregnant women, only one had low avidity IgG corresponding to the acute phase of the infection. ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762008000600013
更新日期:2008-09-01 00:00:00
abstract::Non-bilayer phospholipid arrangements are three-dimensional structures that form when anionic phospholipids with an intermediate structure of the tubular hexagonal phase II are present in a bilayer of lipids. Antibodies that recognise these arrangements have been described in patients with antiphospholipid syndrome an...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762012000900016
更新日期:2012-12-01 00:00:00
abstract::Aedes albifasciatus is an important common mosquito in Central Argentina. It is a confirmed vector of the Western Equine Encephalitis and is responsible for loss of milk production in dairy cattle during peak populations. This paper reports the flight activity pattern of Ae. albifasciatus for different seasons, in the...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761995000500020
更新日期:1995-09-01 00:00:00
abstract::We have been interested in identifying genes that play a role in reproduction of the mosquito Aedes aegypti. Our interests are currently focused on the vitellogenin genes which in the mosquito are expressed only in the fat body in response to the insect steroid hormone, 20-hydroxyecdysone. Four of the five vitellogeni...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761987000700021
更新日期:1987-01-01 00:00:00
abstract::The present report describes an alternative method for in vitro detection of HIV-1-specific antibody secretion in 24h of culture employing as stimulant of peripheral blood mononuclear cells the disrupted inactivated whole virus adsorbed onto microwells in a commercial ELISA kit plates. The results obtained from this t...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761992000200012
更新日期:1992-04-01 00:00:00
abstract::Experimental techniques that we have found useful during our studies of insect blood-feeding behaviour are reviewed. Some of the principal findings resulting from these techniques are discussed. Where directly applicable, the work of others is included, but no complete review of the subject has been attempted. ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章,评审
doi:10.1590/s0074-02761987000700005
更新日期:1987-01-01 00:00:00
abstract::Goats from the Venezuelan northern arid zones were found infested by the following nematodes: Haemonchus contortus, Trichostrongylus axei, T. colubriformis, Cooperia curticei, Oesophagostomum columbianum, Skrjabinema ovis y Trichuris globulosa. The Shannon-Weaver index of diversity values oscillated between 0.045 and ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761986000200009
更新日期:1986-04-01 00:00:00
abstract::The invasion of the erythrocyte by Plasmodium falciparum depends on the ability of the merozoite to move through the membrane invagination. This ability is probably mediated by actin dependent motors. Using affinity columns with G-actin and F-actin we isolated actin binding proteins from the parasite. By immunoblottin...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762000000300007
更新日期:2000-05-01 00:00:00
abstract::Facilitated and improved by advances in molecular biology, techniques for the immunodiagnosis of schistosomiasis, including assays based on the detection of antigens circulating in the serum and/or excreted in the urine, have now reached the stage of multi-centre trials. There is a need to complement parasitological t...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章,评审
doi:10.1590/s0074-02761992000800004
更新日期:1992-01-01 00:00:00
abstract::In C57Bl/6 strain mice vaccinated with radiation-attenuated cercariae of Schistosoma mansoni immune elimination of challenge parasites occurs in the lungs. Leucocytes were recovered from the lungs of such mice by bronchoalveolar lavage and cultured in vitro with larval antigen; the profile of cytokines released was th...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章,评审
doi:10.1590/s0074-02761992000800015
更新日期:1992-01-01 00:00:00
abstract::Sand fly identification is complex because it depends on the expertise of the taxonomist. The females show subtle morphological differences and the occurrence of the species complexes are usual in this taxon. Therefore, a fragment of the cytochrome c oxidase subunit I (COI) gene is used for taxon barcoding to resolve ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-02760190208
更新日期:2019-11-28 00:00:00
abstract::The ultrastructure of endogenous stages of Eimeria ninakohlyakimovae was observed in epithelial cells of cecum and colon crypts from a goat experimentally infected with 2.0 x 10(5) oocysts/kg. The secondary meronts developed above the nucleus of the host cell. The nucleus first divides and merozoites then form on the ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761997000400017
更新日期:1997-07-01 00:00:00
abstract::The purpose of this study was to investigate the genotypes of Giardia lamblia from human and animal feces and their epidemiological and clinical characteristics in Argentina, South America. Seventy isolates, 60 from humans (adults and children), eight from dogs and two from cows were processed by polymerase chain reac...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762008000100015
更新日期:2008-02-01 00:00:00
abstract::The high level of protection elicited in rodents and primates by the radiation-attenuated schistosome vaccine gives hope that a human vaccine relying on equivalent mechanisms is feasible. In humans, a vaccine would be undoubtedly administered to previously or currently infected individuals. We have therefore used the ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02762006000900061
更新日期:2006-09-01 00:00:00
abstract::Different molecular-genetic methods were used to identify a cohort of Leishmania strains from natural foci of zoonotic cutaneous leishmaniasis located in Central Asia, on the former USSR territory. The results obtained using isoenzymes, PCR, restriction fragment length polymorphisms of kDNA and molecular hybridization...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761997000100023
更新日期:1997-01-01 00:00:00
abstract::An important point in paleoparasitology is the correct diagnosis of the origin of coprolites found in archaeological sites. The identification of human and animal coprolites, through the study of the shape, size, characteristics after rehydration, alimentary contents, and the presence of parasites, has proved to be ac...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761989000200007
更新日期:1989-04-01 00:00:00
abstract::An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a pr...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/0074-02760140457
更新日期:2015-06-01 00:00:00
abstract::Small molecular weight compounds from Mandevilla velutina and from Eclipta prostata were found to be active against snakebite. ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761991000600044
更新日期:1991-01-01 00:00:00
abstract::Preparations of simian rotavirus SA11 maintained in different laboratories were compared with each other by polyacrylamide gel electrophoresis of genomic RNA. Differences in the migration of genome segments 4, 5 and 7 allowed the classification of eight virus preparations into four electrophoretic types. ...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761986000400005
更新日期:1986-10-01 00:00:00
abstract::Lapachol was chemically modified to obtain its thiosemicarbazone and semicarbazone derivatives. These compounds were tested for antimicrobial activity against several bacteria and fungi by the broth microdilution method. The thiosemicarbazone and semicarbazone derivatives of lapachol exhibited antimicrobial activity a...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/S0074-02762013000300013
更新日期:2013-05-01 00:00:00
abstract::Soluble antigens (Ag) from Paracoccidioides brasiliensis, Histoplasma capsulatum and Aspergillus fumigatus were prepared and standardized by double immunodiffusion (DID) and immunoelectroosmophoresis (IEOP). No difference in sensitivity was observed between the two techniques; 100% of standard patient sera were positi...
journal_title:Memorias do Instituto Oswaldo Cruz
pub_type: 杂志文章
doi:10.1590/s0074-02761985000300005
更新日期:1985-07-01 00:00:00