Neutrophil CD64 expression levels in IGRA-positive individuals distinguish latent tuberculosis from active disease.

Abstract:

BACKGROUND:CD64 (FcγR1) is a high-affinity receptor for monomeric IgG1 and IgG3. Circulating neutrophils express very low amounts of CD64 on their surface. OBJECTIVES:Our primary aim was to investigate the utility of neutrophil CD64 surface expression as a biomarker of active pulmonary tuberculosis (TB). We hypothesised that elevated neutrophil CD64 expression in TB infection would be associated with interferon gamma (IFN-γ) as an inducer of CD64 expression. METHODS:The expression level of CD64 per neutrophil (PMN CD64 index) was quantitatively measured with flow cytometry using a Leuko64 kit in samples from patients with TB and latent TB infection (LTBI) as well as healthy controls, as part of a prospective cohort study in Brazil. FINDINGS:The PMN CD64 index in patients with TB was higher than that in healthy controls and LTBI. Receiver operating characteristic curve analyses determined that the PMN CD64 index could discriminate patients with TB from those with LTBI and healthy individuals. PMN CD64 index levels returned to baseline levels after treatment. CONCLUSIONS:The positive regulation of CD64 expression in circulating neutrophils of patients with active TB could represent an additional biomarker for diagnosis of active TB and could be used for monitoring individuals with LTBI before progression of TB disease.

journal_name

Mem Inst Oswaldo Cruz

authors

Corrêa RDS,Rodrigues LS,Pereira LHL,Nogueira OC,Leung J,Sousa MDS,Hacker MA,Siqueira HR,Capone D,Alves RLR,Pessolani MCV,Schmitz V,Pereira GMB

doi

10.1590/0074-02760180579

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

e180579

eissn

0074-0276

issn

1678-8060

pii

S0074-02762019000100319

journal_volume

114

pub_type

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