Purification and biochemical characterization of four iron superoxide dismutases in Trypanosoma cruzi.

Abstract:

:Four superoxide dismutase (SOD) activities (SOD I, II, III, and IV) have been characterized in the epimastigote form of Trypanosoma cruzi. The total extract was subjected to two successive ammonium sulphate additions between 35 and 85%, and the resulting fraction was purified using two continuous chromatography processes (ion exchange and filtration). Enzymes were insensitive to cyanide but sensitive to hydrogen peroxide, properties characteristic of iron-containing SODs. The molecular masses of the different SODs were 20 kDa (SOD I), 60 kDa (SOD II), 50 kDa (SOD III) and 25 kDa (SOD IV), whereas the isoelectric points were 6.9, 6.8, 5.2 and 3.8, respectively. Subcellular location and digitonin experiments have shown that these SODs are mainly cytosolic, with small amounts in the low-mass organelles (SOD II and SOD I) and the mitochondrion (SOD III), where these enzymes play an important role in minimizing oxidative damage.

journal_name

Mem Inst Oswaldo Cruz

authors

Mateo H,Marín C,Pérez-Cordón G,Sánchez-Moreno M

doi

10.1590/s0074-02762008000300008

subject

Has Abstract

pub_date

2008-05-01 00:00:00

pages

271-6

issue

3

eissn

0074-0276

issn

1678-8060

pii

S0074-02762008000300008

journal_volume

103

pub_type

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