Developmentally Regulated Expression of the Gene Family for Cytosolic Glutamine Synthetase in Pisum sativum.

Abstract:

:In Pisum sativum, two classes of genes encode distinct isoforms of cytosolic glutamine synthetase (GS). The first class comprises two nearly identical or "twin" GS genes (GS341 and GS132), while the second comprises a single GS gene (GS299) distinct in both coding and noncoding regions from the "twin" GS genes. Gene-specific analyses were used to monitor the individual contribution of each gene for cytosolic GS during root nodule development and in cotyledons during germination, two contexts where large amounts of ammonia must be assimilated by GS for nitrogen transport. mRNAs corresponding to all three genes for cytosolic GS were shown to accumulate coordinately during a time course of nodule development. All the GS mRNAs also accumulate to wild-type levels in mutant nodules formed by a nifD(-) strain of Rhizobium leguminosarum indicating that induced GS expression in pea root nodules does not depend on the production of ammonia. Distinct patterns of expression for the two classes of GS genes were observed in certain mutant root nodules and most dramatically in cotyledons of germinating seedlings. The different patterns of expression between the two classes of genes for cytosolic GS suggests that their distinct gene products may serve nonoverlapping functions during pea development.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Walker EL,Coruzzi GM

doi

10.1104/pp.91.2.702

subject

Has Abstract

pub_date

1989-10-01 00:00:00

pages

702-8

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

91

pub_type

杂志文章
  • Light Quality and Irradiance Level Interaction in the Control of Expression of Light-Harvesting Complex of Photosystem II: Pigments, Pigment-Proteins, and mRNA Accumulation.

    abstract::Effects of red and blue light at irradiances from 1.6 to 28.3 micromolar per square meter per second on chloroplast pigments, light-harvesting pigment-proteins associated with photosystem II, and the corresponding mRNA were evaluated in maize (Zea mays L.) plants (OP Golden Bantum) grown for 14 days under 14 hours lig...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.1.163

    authors: Eskins K,Westhoff P,Beremand PD

    更新日期:1989-09-01 00:00:00

  • Calcium-regulated proteolysis of eEF1A.

    abstract::Eukaryotic elongation factor 1alpha (eEF1A) can be post-translationally modified by the addition of phosphorylglycerylethanolamine (PGE). [(14)C]Ethanolamine was incorporated into the PGE modification, and with carrot (Daucus carota L.) suspension culture cells, eEF1A was the only protein that incorporated detectable ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.3.957

    authors: Ransom-Hodgkins WD,Brglez I,Wang X,Boss WF

    更新日期:2000-03-01 00:00:00

  • Spatial and temporal distribution of polyamine levels and polyamine anabolism in different organs/tissues of the tobacco plant. Correlations with age, cell division/expansion, and differentiation.

    abstract::Polyamine (PA) titers and biosynthesis follow a basipetal decrease along the tobacco (Nicotiana tabacum) plant axis, and they also correlate negatively with cell size. On the contrary, the titers of arginine (Arg), ornithine (Orn), and arginase activity increase with age. The free (soluble)/total-PA ratios gradually i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.055483

    authors: Paschalidis KA,Roubelakis-Angelakis KA

    更新日期:2005-05-01 00:00:00

  • Acclimation to High CO2 Requires the ω Subunit of the RNA Polymerase in Synechocystis.

    abstract::Inactivation of the nonessential ω-subunit of the RNA polymerase core in the ΔrpoZ strain of the model cyanobacterium Synechocystis sp. PCC 6803 leads to a unique high-CO2-sensitive phenotype. Supplementing air in the growth chamber with 30 mL L-1 (3%) CO2 accelerated the growth rate of the control strain (CS) 4-fold,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01953

    authors: Kurkela J,Hakkila K,Antal T,Tyystjärvi T

    更新日期:2017-05-01 00:00:00

  • Relationship between Respiration and Photosynthesis in Guard Cell and Mesophyll Cell Protoplasts of Commelina communis L.

    abstract::A mass spectrometric method combining (16)O/(18)O and (12)C/(13)C isotopes was used to quantify the unidirectional fluxes of O(2) and CO(2) during a dark to light transition for guard cell protoplasts and mesophyll cell protoplasts of Commelina communis L. In darkness, O(2) uptake and CO(2) evolution were similar on a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.2.636

    authors: Gautier H,Vavasseur A,Gans P,Lascève G

    更新日期:1991-02-01 00:00:00

  • A cross-polarization, magic-angle-spinning, 13C-nuclear-magnetic-resonance study of polysaccharides in sugar beet cell walls

    abstract::Solid-state nuclear magnetic resonance relaxation experiments were used to study the rigidity and spatial proximity of polymers in sugar beet (Beta vulgaris) cell walls. Proton T1rho decay and cross-polarization patterns were consistent with the presence of rigid, crystalline cellulose microfibrils with a diameter of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.119.4.1315

    authors: Renard CM,Jarvis MC

    更新日期:1999-04-01 00:00:00

  • Ricinosomes predict programmed cell death leading to anther dehiscence in tomato.

    abstract::Successful development and dehiscence of the anther and release of pollen are dependent upon the programmed cell death (PCD) of the tapetum and other sporophytic tissues. Ultrastructural examination of the developing and dehiscing anther of tomato (Solanum lycopersicum) revealed that cells of the interlocular septum, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.132720

    authors: Senatore A,Trobacher CP,Greenwood JS

    更新日期:2009-02-01 00:00:00

  • DIACYLGLYCEROL ACYLTRANSFERASE and DIACYLGLYCEROL KINASE Modulate Triacylglycerol and Phosphatidic Acid Production in the Plant Response to Freezing Stress.

    abstract::Plants accumulate the lipids phosphatidic acid (PA), diacylglycerol (DAG), and triacylglycerol (TAG) during cold stress, but how plants balance the levels of these lipids to mediate cold responses remains unknown. The enzymes ACYL-COENZYME A:DIACYLGLYCEROL ACYLTRANSFERASE (DGAT) and DIACYLGLYCEROL KINASE (DGK) catalyz...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.00402

    authors: Tan WJ,Yang YC,Zhou Y,Huang LP,Xu L,Chen QF,Yu LJ,Xiao S

    更新日期:2018-07-01 00:00:00

  • A Starch Deficient Mutant of Arabidopsis thaliana with Low ADPglucose Pyrophosphorylase Activity Lacks One of the Two Subunits of the Enzyme.

    abstract::A starch deficient mutant of Arabidopsis thaliana (L.) Heynh. has been isolated in which leaf extracts contain only about 5% as much activity of ADPglucose pyrophosphorylase (EC 2.7.7.27) as the wild type. A single, nuclear mutation at a previously undescribed locus designated adg2 is responsible for the mutant phenot...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.4.1175

    authors: Lin TP,Caspar T,Somerville CR,Preiss J

    更新日期:1988-12-01 00:00:00

  • Light and the correlation of chloroplast development and coupling of phosphorylation to electron transport.

    abstract::Coupling of phosphorylation to electron transport was examined by measuring the photosynthetic control ratio for broken wheat plastids isolated from seedlings at different greening stages. The photosynthetic control ratio progressively increased during greening and tight coupling was noted after granal stacking and th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.5.880

    authors: Duysen ME,Freeman TP,Zabrocki RD

    更新日期:1980-05-01 00:00:00

  • A novel image-analysis technique for kinematic study of growth and curvature.

    abstract::Kinematic analysis has provided important insights into the biology of growth by revealing the distribution of expansion within growing organs. Modern methods of kinematic analysis have made use of new image-tracking algorithms and computer-assisted evaluation, but these methods have yet to be adapted for examination ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.103226

    authors: Basu P,Pal A,Lynch JP,Brown KM

    更新日期:2007-10-01 00:00:00

  • Tobacco Plants Transformed with the Bean alphaai Gene Express an Inhibitor of Insect alpha-Amylase in Their Seeds.

    abstract::Bean (Phaseolus vulgaris L.) seeds contain a putative plant defense protein that inhibits insect and mammalian but not plant alpha-amylases. We recently (J Moreno, MJ Chrispeels [1989] Proc Natl Acad Sci USA 86:7885-7889) presented strong circumstantial evidence that this alpha-amylase inhibitor (alphaAI) is encoded b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.805

    authors: Altabella T,Chrispeels MJ

    更新日期:1990-06-01 00:00:00

  • Diurnal Changes in Metabolite Levels and Crassulacean Acid Metabolism in Kalanchoë daigremontiana Leaves.

    abstract::Diurnal changes in levels of selected metabolites associated with glycolysis, the C(3) cycle, C(4)-organic acids, and storage carbohydrates were analyzed in active Kalanchoë daigremontiana Crassulacean acid metabolism leaves. Three metabolic transition periods occurred each day. During the first two hours of light, ne...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.5.1002

    authors: Kenyon WH,Holaday AS,Black CC

    更新日期:1981-11-01 00:00:00

  • Analysis of Porphyra membrane transporters demonstrates gene transfer among photosynthetic eukaryotes and numerous sodium-coupled transport systems.

    abstract::Membrane transporters play a central role in many cellular processes that rely on the movement of ions and organic molecules between the environment and the cell, and between cellular compartments. Transporters have been well characterized in plants and green algae, but little is known about transporters or their evol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.193896

    authors: Chan CX,Zäuner S,Wheeler G,Grossman AR,Prochnik SE,Blouin NA,Zhuang Y,Benning C,Berg GM,Yarish C,Eriksen RL,Klein AS,Lin S,Levine I,Brawley SH,Bhattacharya D

    更新日期:2012-04-01 00:00:00

  • Passive nitrate transport by root plasma membrane vesicles exhibits an acidic optimal pH like the H(+)-ATPase.

    abstract::The net initial passive flux (J(Ni)) in reconstituted plasma membrane (PM) vesicles from maize (Zea mays) root cells was measured as recently described (P. Pouliquin, J.-P. Grouzis, R. Gibrat ¿1999 Biophys J 76: 360-373). J(Ni) in control liposomes responded to membrane potential or to NO(3)(-) as expected from the Go...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.1.265

    authors: Pouliquin P,Boyer JC,Grouzis JP,Gibrat R

    更新日期:2000-01-01 00:00:00

  • Reconfiguration of the achene and receptacle metabolic networks during strawberry fruit development.

    abstract::The anatomy of strawberry (Fragaria x ananassa) fruit, in which the achene is found on the outer part of the fruit, makes it an excellent species for studying the regulation of fruit development. It can provide a model for the cross talk between primary and secondary metabolism, whose role is of pivotal importance in ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.120691

    authors: Fait A,Hanhineva K,Beleggia R,Dai N,Rogachev I,Nikiforova VJ,Fernie AR,Aharoni A

    更新日期:2008-10-01 00:00:00

  • Effects of ethylene, kinetin, and calcium on growth and wall composition of pea epicotyls.

    abstract::Ethylene supplied with indoleacetic acid at 0.1 and 1 mum inhibited elongation and enhanced swelling in epicotyls of decapitated and derooted pea seedlings (Pisum sativum L., var. Alaska). These growth responses were correlated with the development of cell walls rich in weak acid-extractable materials and pectic uroni...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.55.3.450

    authors: Mondal MH,Nance JF

    更新日期:1975-03-01 00:00:00

  • Regulation of a Cytochrome P450 Gene CYP94B1 by WRKY33 Transcription Factor Controls Apoplastic Barrier Formation in Roots to Confer Salt Tolerance.

    abstract::Salinity is an environmental stress that causes decline in crop yield. Avicennia officinalis and other mangroves have adaptations such as ultrafiltration at the roots aided by apoplastic cell wall barriers to thrive in saline conditions. We studied a cytochrome P450 gene from A. officinalis, AoCYP94B1, and its putativ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.01054

    authors: Krishnamurthy P,Vishal B,Ho WJ,Lok FCJ,Lee FSM,Kumar PP

    更新日期:2020-12-01 00:00:00

  • Functional genomic analysis supports conservation of function among cellulose synthase-like a gene family members and suggests diverse roles of mannans in plants.

    abstract::Mannan polysaccharides are widespread among plants, where they serve as structural elements in cell walls, as carbohydrate reserves, and potentially perform other important functions. Previous work has demonstrated that members of the cellulose synthase-like A (CslA) family of glycosyltransferases from Arabidopsis (Ar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.093989

    authors: Liepman AH,Nairn CJ,Willats WG,Sørensen I,Roberts AW,Keegstra K

    更新日期:2007-04-01 00:00:00

  • The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis.

    abstract::In many plant species, a subset of the genes of the chloroplast genome is transcribed by RpoTp, a nuclear-encoded plastid-targeted RNA polymerase. Here, we describe the positional cloning of the SCABRA3 (SCA3) gene, which was found to encode RpoTp in Arabidopsis (Arabidopsis thaliana). We studied one weak (sca3-1) and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.080069

    authors: Hricová A,Quesada V,Micol JL

    更新日期:2006-07-01 00:00:00

  • Embryo Development in Phaseolus vulgaris: II. Analysis of Selected Inorganic Ions, Ammonia, Organic Acids, Amino Acids, and Sugars in the Endosperm Liquid.

    abstract::Endosperm liquid bathes the embryo of Phaseolus vulgaris from the heart stage through the late cotyledon state. This liquid was aspirated from many ovules of the same stage, pooled, and analyzed for the following constituents and parameters: [List: see text]. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.51.3.454

    authors: Smith JG

    更新日期:1973-03-01 00:00:00

  • Selection for Improved Energy Use Efficiency and Drought Tolerance in Canola Results in Distinct Transcriptome and Epigenome Changes.

    abstract::To increase both the yield potential and stability of crops, integrated breeding strategies are used that have mostly a direct genetic basis, but the utility of epigenetics to improve complex traits is unclear. A better understanding of the status of the epigenome and its contribution to agronomic performance would he...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00155

    authors: Verkest A,Byzova M,Martens C,Willems P,Verwulgen T,Slabbinck B,Rombaut D,Van de Velde J,Vandepoele K,Standaert E,Peeters M,Van Lijsebettens M,Van Breusegem F,De Block M

    更新日期:2015-08-01 00:00:00

  • Ubiquitin-Proteasome Dependent Regulation of the GOLDEN2-LIKE 1 Transcription Factor in Response to Plastid Signals.

    abstract::Arabidopsis (Arabidopsis thaliana) GOLDEN2-LIKE (GLK) transcription factors promote chloroplast biogenesis by regulating the expression of photosynthesis-related genes. Arabidopsis GLK1 is also known to participate in retrograde signaling from chloroplasts to the nucleus. To elucidate the mechanism by which GLK1 is re...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01546

    authors: Tokumaru M,Adachi F,Toda M,Ito-Inaba Y,Yazu F,Hirosawa Y,Sakakibara Y,Suiko M,Kakizaki T,Inaba T

    更新日期:2017-01-01 00:00:00

  • StGCPRP, a potato gene strongly expressed in stomatal guard cells, defines a novel type of repetitive proline-rich proteins.

    abstract::Guard cells represent a highly differentiated cell type within the epidermis of plant leaves and stems. They respond to many endogenous and environmental signals and thereby modify the size of the stomatal pore they surround. We identified a novel gene that is highly expressed in guard cells of potato (Solanum tuberos...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.3.677

    authors: Menke U,Renault N,Mueller-Roeber B

    更新日期:2000-03-01 00:00:00

  • Single-Cell RNA Sequencing Resolves Molecular Relationships Among Individual Plant Cells.

    abstract::Single-cell RNA sequencing (scRNA-seq) has been used extensively to study cell-specific gene expression in animals, but it has not been widely applied to plants. Here, we describe the use of a commercially available droplet-based microfluidics platform for high-throughput scRNA-seq to obtain single-cell transcriptomes...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01482

    authors: Ryu KH,Huang L,Kang HM,Schiefelbein J

    更新日期:2019-04-01 00:00:00

  • Water-stress-induced ethylene production in wheat : a fact or artifact?

    abstract::Effects of water stress on ethylene evolution from excised leaf segments and intact plants of wheat (Triticum aestivum L. cv Katepwa) were studied. Excised leaf segments of 8 day or 6 week old plants were dried until they lost 8% of their fresh weight (water potential about -2.3 megapascals). These and nondried contro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.2.406

    authors: Narayana I,Lalonde S,Saini HS

    更新日期:1991-06-01 00:00:00

  • Characterization of two isoenzymes of lipoxygenase from bush beans.

    abstract::Two isoenzymes of lipoxygenase, a and b, have been obtained from bush beans (Phaseolus vulgaris) as electrophoretically homogeneous proteins. Both proteins have a molecular weight of 100,000, contain 1 atom of iron, and appear to be composed of a single peptide chain. However, these enzymes appear to differ in some ot...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.4.695

    authors: Hurt GB,Axelrod B

    更新日期:1977-04-01 00:00:00

  • Photosynthetic carbon metabolism of isolated corn chloroplasts.

    abstract::Chloroplasts have been isolated from 4- to 6-day-old corn (Zea mays) leaves capable of assimilating 45 micromoles CO(2) per milligram chlorophyll per hour. The effects of various factors such as inorganic phosphate, reducing agents, inhibitors, intermediates of the photosynthetic carbon reduction cycle, organic acids,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.49.4.607

    authors: O'neal D,Hew CS,Latzko E,Gibbs M

    更新日期:1972-04-01 00:00:00

  • Cluster bean-a ureide- or amide-producing legume?

    abstract::Xylem sap of cluster bean (Cyamopsis tetragonoloba L. cv FS-277) and pigeonpea (Cajanus cajan cv UPAS-120) were analyzed for total nitrogen, amide nitrogen, and ureide nitrogen at flowering stage. Nitrogenase, uricase, and allantoinase were compared in nodules of cluster bean and pigeonpea. Xylem sap of cluster bean e...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.3.917

    authors: Sheoran IS,Luthra YP,Kuhad MS,Singh R

    更新日期:1982-09-01 00:00:00

  • Characterization of P69E and P69F, two differentially regulated genes encoding new members of the subtilisin-like proteinase family from tomato plants.

    abstract::Subtilisin-like proteins represent an ancient family of serine proteases that are extremely widespread in living organisms. We report here the structure and genomic organization of two new transcriptionally active genes encoding proteins that belong to the P69 family of subtilisin-like proteases from tomato (Lycopersi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.122.1.67

    authors: Jordá L,Conejero V,Vera P

    更新日期:2000-01-01 00:00:00