Abstract:
:In Pisum sativum, two classes of genes encode distinct isoforms of cytosolic glutamine synthetase (GS). The first class comprises two nearly identical or "twin" GS genes (GS341 and GS132), while the second comprises a single GS gene (GS299) distinct in both coding and noncoding regions from the "twin" GS genes. Gene-specific analyses were used to monitor the individual contribution of each gene for cytosolic GS during root nodule development and in cotyledons during germination, two contexts where large amounts of ammonia must be assimilated by GS for nitrogen transport. mRNAs corresponding to all three genes for cytosolic GS were shown to accumulate coordinately during a time course of nodule development. All the GS mRNAs also accumulate to wild-type levels in mutant nodules formed by a nifD(-) strain of Rhizobium leguminosarum indicating that induced GS expression in pea root nodules does not depend on the production of ammonia. Distinct patterns of expression for the two classes of GS genes were observed in certain mutant root nodules and most dramatically in cotyledons of germinating seedlings. The different patterns of expression between the two classes of genes for cytosolic GS suggests that their distinct gene products may serve nonoverlapping functions during pea development.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Walker EL,Coruzzi GMdoi
10.1104/pp.91.2.702subject
Has Abstractpub_date
1989-10-01 00:00:00pages
702-8issue
2eissn
0032-0889issn
1532-2548journal_volume
91pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Effects of red and blue light at irradiances from 1.6 to 28.3 micromolar per square meter per second on chloroplast pigments, light-harvesting pigment-proteins associated with photosystem II, and the corresponding mRNA were evaluated in maize (Zea mays L.) plants (OP Golden Bantum) grown for 14 days under 14 hours lig...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.1.163
更新日期:1989-09-01 00:00:00
abstract::Eukaryotic elongation factor 1alpha (eEF1A) can be post-translationally modified by the addition of phosphorylglycerylethanolamine (PGE). [(14)C]Ethanolamine was incorporated into the PGE modification, and with carrot (Daucus carota L.) suspension culture cells, eEF1A was the only protein that incorporated detectable ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.3.957
更新日期:2000-03-01 00:00:00
abstract::Polyamine (PA) titers and biosynthesis follow a basipetal decrease along the tobacco (Nicotiana tabacum) plant axis, and they also correlate negatively with cell size. On the contrary, the titers of arginine (Arg), ornithine (Orn), and arginase activity increase with age. The free (soluble)/total-PA ratios gradually i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.055483
更新日期:2005-05-01 00:00:00
abstract::Inactivation of the nonessential ω-subunit of the RNA polymerase core in the ΔrpoZ strain of the model cyanobacterium Synechocystis sp. PCC 6803 leads to a unique high-CO2-sensitive phenotype. Supplementing air in the growth chamber with 30 mL L-1 (3%) CO2 accelerated the growth rate of the control strain (CS) 4-fold,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01953
更新日期:2017-05-01 00:00:00
abstract::A mass spectrometric method combining (16)O/(18)O and (12)C/(13)C isotopes was used to quantify the unidirectional fluxes of O(2) and CO(2) during a dark to light transition for guard cell protoplasts and mesophyll cell protoplasts of Commelina communis L. In darkness, O(2) uptake and CO(2) evolution were similar on a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.95.2.636
更新日期:1991-02-01 00:00:00
abstract::Solid-state nuclear magnetic resonance relaxation experiments were used to study the rigidity and spatial proximity of polymers in sugar beet (Beta vulgaris) cell walls. Proton T1rho decay and cross-polarization patterns were consistent with the presence of rigid, crystalline cellulose microfibrils with a diameter of ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.4.1315
更新日期:1999-04-01 00:00:00
abstract::Successful development and dehiscence of the anther and release of pollen are dependent upon the programmed cell death (PCD) of the tapetum and other sporophytic tissues. Ultrastructural examination of the developing and dehiscing anther of tomato (Solanum lycopersicum) revealed that cells of the interlocular septum, ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.132720
更新日期:2009-02-01 00:00:00
abstract::Plants accumulate the lipids phosphatidic acid (PA), diacylglycerol (DAG), and triacylglycerol (TAG) during cold stress, but how plants balance the levels of these lipids to mediate cold responses remains unknown. The enzymes ACYL-COENZYME A:DIACYLGLYCEROL ACYLTRANSFERASE (DGAT) and DIACYLGLYCEROL KINASE (DGK) catalyz...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.00402
更新日期:2018-07-01 00:00:00
abstract::A starch deficient mutant of Arabidopsis thaliana (L.) Heynh. has been isolated in which leaf extracts contain only about 5% as much activity of ADPglucose pyrophosphorylase (EC 2.7.7.27) as the wild type. A single, nuclear mutation at a previously undescribed locus designated adg2 is responsible for the mutant phenot...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.4.1175
更新日期:1988-12-01 00:00:00
abstract::Coupling of phosphorylation to electron transport was examined by measuring the photosynthetic control ratio for broken wheat plastids isolated from seedlings at different greening stages. The photosynthetic control ratio progressively increased during greening and tight coupling was noted after granal stacking and th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.5.880
更新日期:1980-05-01 00:00:00
abstract::Kinematic analysis has provided important insights into the biology of growth by revealing the distribution of expansion within growing organs. Modern methods of kinematic analysis have made use of new image-tracking algorithms and computer-assisted evaluation, but these methods have yet to be adapted for examination ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.103226
更新日期:2007-10-01 00:00:00
abstract::Bean (Phaseolus vulgaris L.) seeds contain a putative plant defense protein that inhibits insect and mammalian but not plant alpha-amylases. We recently (J Moreno, MJ Chrispeels [1989] Proc Natl Acad Sci USA 86:7885-7889) presented strong circumstantial evidence that this alpha-amylase inhibitor (alphaAI) is encoded b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.2.805
更新日期:1990-06-01 00:00:00
abstract::Diurnal changes in levels of selected metabolites associated with glycolysis, the C(3) cycle, C(4)-organic acids, and storage carbohydrates were analyzed in active Kalanchoë daigremontiana Crassulacean acid metabolism leaves. Three metabolic transition periods occurred each day. During the first two hours of light, ne...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.5.1002
更新日期:1981-11-01 00:00:00
abstract::Membrane transporters play a central role in many cellular processes that rely on the movement of ions and organic molecules between the environment and the cell, and between cellular compartments. Transporters have been well characterized in plants and green algae, but little is known about transporters or their evol...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.193896
更新日期:2012-04-01 00:00:00
abstract::The net initial passive flux (J(Ni)) in reconstituted plasma membrane (PM) vesicles from maize (Zea mays) root cells was measured as recently described (P. Pouliquin, J.-P. Grouzis, R. Gibrat ¿1999 Biophys J 76: 360-373). J(Ni) in control liposomes responded to membrane potential or to NO(3)(-) as expected from the Go...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.1.265
更新日期:2000-01-01 00:00:00
abstract::The anatomy of strawberry (Fragaria x ananassa) fruit, in which the achene is found on the outer part of the fruit, makes it an excellent species for studying the regulation of fruit development. It can provide a model for the cross talk between primary and secondary metabolism, whose role is of pivotal importance in ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.120691
更新日期:2008-10-01 00:00:00
abstract::Ethylene supplied with indoleacetic acid at 0.1 and 1 mum inhibited elongation and enhanced swelling in epicotyls of decapitated and derooted pea seedlings (Pisum sativum L., var. Alaska). These growth responses were correlated with the development of cell walls rich in weak acid-extractable materials and pectic uroni...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.3.450
更新日期:1975-03-01 00:00:00
abstract::Salinity is an environmental stress that causes decline in crop yield. Avicennia officinalis and other mangroves have adaptations such as ultrafiltration at the roots aided by apoplastic cell wall barriers to thrive in saline conditions. We studied a cytochrome P450 gene from A. officinalis, AoCYP94B1, and its putativ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.01054
更新日期:2020-12-01 00:00:00
abstract::Mannan polysaccharides are widespread among plants, where they serve as structural elements in cell walls, as carbohydrate reserves, and potentially perform other important functions. Previous work has demonstrated that members of the cellulose synthase-like A (CslA) family of glycosyltransferases from Arabidopsis (Ar...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.093989
更新日期:2007-04-01 00:00:00
abstract::In many plant species, a subset of the genes of the chloroplast genome is transcribed by RpoTp, a nuclear-encoded plastid-targeted RNA polymerase. Here, we describe the positional cloning of the SCABRA3 (SCA3) gene, which was found to encode RpoTp in Arabidopsis (Arabidopsis thaliana). We studied one weak (sca3-1) and...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.080069
更新日期:2006-07-01 00:00:00
abstract::Endosperm liquid bathes the embryo of Phaseolus vulgaris from the heart stage through the late cotyledon state. This liquid was aspirated from many ovules of the same stage, pooled, and analyzed for the following constituents and parameters: [List: see text]. ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.51.3.454
更新日期:1973-03-01 00:00:00
abstract::To increase both the yield potential and stability of crops, integrated breeding strategies are used that have mostly a direct genetic basis, but the utility of epigenetics to improve complex traits is unclear. A better understanding of the status of the epigenome and its contribution to agronomic performance would he...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00155
更新日期:2015-08-01 00:00:00
abstract::Arabidopsis (Arabidopsis thaliana) GOLDEN2-LIKE (GLK) transcription factors promote chloroplast biogenesis by regulating the expression of photosynthesis-related genes. Arabidopsis GLK1 is also known to participate in retrograde signaling from chloroplasts to the nucleus. To elucidate the mechanism by which GLK1 is re...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01546
更新日期:2017-01-01 00:00:00
abstract::Guard cells represent a highly differentiated cell type within the epidermis of plant leaves and stems. They respond to many endogenous and environmental signals and thereby modify the size of the stomatal pore they surround. We identified a novel gene that is highly expressed in guard cells of potato (Solanum tuberos...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.3.677
更新日期:2000-03-01 00:00:00
abstract::Single-cell RNA sequencing (scRNA-seq) has been used extensively to study cell-specific gene expression in animals, but it has not been widely applied to plants. Here, we describe the use of a commercially available droplet-based microfluidics platform for high-throughput scRNA-seq to obtain single-cell transcriptomes...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.18.01482
更新日期:2019-04-01 00:00:00
abstract::Effects of water stress on ethylene evolution from excised leaf segments and intact plants of wheat (Triticum aestivum L. cv Katepwa) were studied. Excised leaf segments of 8 day or 6 week old plants were dried until they lost 8% of their fresh weight (water potential about -2.3 megapascals). These and nondried contro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.2.406
更新日期:1991-06-01 00:00:00
abstract::Two isoenzymes of lipoxygenase, a and b, have been obtained from bush beans (Phaseolus vulgaris) as electrophoretically homogeneous proteins. Both proteins have a molecular weight of 100,000, contain 1 atom of iron, and appear to be composed of a single peptide chain. However, these enzymes appear to differ in some ot...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.59.4.695
更新日期:1977-04-01 00:00:00
abstract::Chloroplasts have been isolated from 4- to 6-day-old corn (Zea mays) leaves capable of assimilating 45 micromoles CO(2) per milligram chlorophyll per hour. The effects of various factors such as inorganic phosphate, reducing agents, inhibitors, intermediates of the photosynthetic carbon reduction cycle, organic acids,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.49.4.607
更新日期:1972-04-01 00:00:00
abstract::Xylem sap of cluster bean (Cyamopsis tetragonoloba L. cv FS-277) and pigeonpea (Cajanus cajan cv UPAS-120) were analyzed for total nitrogen, amide nitrogen, and ureide nitrogen at flowering stage. Nitrogenase, uricase, and allantoinase were compared in nodules of cluster bean and pigeonpea. Xylem sap of cluster bean e...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.3.917
更新日期:1982-09-01 00:00:00
abstract::Subtilisin-like proteins represent an ancient family of serine proteases that are extremely widespread in living organisms. We report here the structure and genomic organization of two new transcriptionally active genes encoding proteins that belong to the P69 family of subtilisin-like proteases from tomato (Lycopersi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.1.67
更新日期:2000-01-01 00:00:00