Calcium-regulated proteolysis of eEF1A.

Abstract:

:Eukaryotic elongation factor 1alpha (eEF1A) can be post-translationally modified by the addition of phosphorylglycerylethanolamine (PGE). [(14)C]Ethanolamine was incorporated into the PGE modification, and with carrot (Daucus carota L.) suspension culture cells, eEF1A was the only protein that incorporated detectable quantities of [(14)C]ethanolamine (Ransom et al., 1998). When 1 mM CaCl(2) was added to microsomes containing [(14)C]ethanolamine-labeled eEF1A ([(14)C]et-eEF1A), there was a 60% decrease in the amount of [(14)C]et-eEF1A recovered after 10 min. The loss of endogenous [(14)C]et-eEF1A was prevented by adding EGTA. Recombinant eEF1A, which did not contain the PGE modification, also was degraded by microsomes in a Ca(2+)-regulated manner, indicating that PGE modification was not necessary for proteolysis; however, it enabled us to quantify enodgenous eEF1A. By monitoring [(14)C]et-eEF1A, we found that treatment with phospholipase D or C, but not phospholipase A(2), resulted in a decrease in [(14)C]et-eEF1A from carrot microsomes. The fact that there was no loss of [(14)C]et-eEF1A with phospholipase A(2) treatment even in the presence of 1 mM Ca(2+) suggested that the loss of membrane lipids was not essential for eEF1A proteolysis and that lysolipids or fatty acids decreased proteolysis. At micromolar Ca(2+) concentrations, proteolysis of eEF1A was pH sensitive. When 1 microM CaCl(2) was added at pH 7.2, 35% of [(14)C]et-eEF1A was lost; while at pH 6.8, 10 microM CaCl(2) was required to give a similar loss of protein. These data suggest that eEF1A may be an important downstream target for Ca(2+) and lipid-mediated signal transduction cascades.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Ransom-Hodgkins WD,Brglez I,Wang X,Boss WF

doi

10.1104/pp.122.3.957

subject

Has Abstract

pub_date

2000-03-01 00:00:00

pages

957-65

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

122

pub_type

杂志文章
  • Characteristics of glutamate dehydrogenase in mitochondria prepared from corn shoots.

    abstract::The amination of alpha-ketoglutarate (alpha-KG) by NADH-glutamate dehydrogenase (GDH) obtained from Sephadex G-75 treated crude extracts from shoots of 5-day-old seedlings was stimulated by the addition of Ca(2+). The NADH-GDH purified 161-fold with ammonium sulfate, DEAE-Toyopearl, and Sephadex G-200 was also activat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.4.1009

    authors: Yamaya T,Oaks A,Matsumoto H

    更新日期:1984-12-01 00:00:00

  • The Influence of 0.03% Carbon Dioxide on Protein Metabolism of Etiolated Avena sativa Coleoptiles.

    abstract::The influence of indoleacetic acid, 0.03% CO(2), and malate on protein metabolism of etiolated Avena sativa coleoptile sections has been investigated. All three were found to elevate both the rate of incorporation of labeled leucine into protein, and the level of soluble protein. The combination of indoleacetic acid a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.1.19

    authors: Bown AW,Aung T

    更新日期:1974-07-01 00:00:00

  • Oxidative Phosphorylation in Germinating Lettuce Seeds (Lactuca sativa) during the First Hours of Imbibition.

    abstract::Experiments with lettuce seeds during the first hours of imbibition showed that oxygen is necessary to sustain high adenine nucleotide ratios and consequently, energy charge values are higher than 0.8 as is usually the case in normally metabolizing tissues.The energy charge value (0.2) of dry seeds soaked in aerated w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.3.631

    authors: Hourmant A,Pradet A

    更新日期:1981-09-01 00:00:00

  • Influence of assimilate demand on photosynthesis, diffusive resistances, translocation, and carbohydrate levels of soybean leaves.

    abstract::Rates of net photosynthesis and translocation, CO(2) diffusive resistances, levels of carbohydrates, total protein, chlorophyll, and inorganic phosphate, and ribulose 1,5-diphosphate carboxylase activity were measured in soybean (Glycine max L. Merrill) leaves to ascertain the effect of altered assimilate demand. To i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.2.201

    authors: Thorne JH,Koller HR

    更新日期:1974-08-01 00:00:00

  • Carotenogenesis Is Regulated by 5'UTR-Mediated Translation of Phytoene Synthase Splice Variants.

    abstract::Phytoene synthase (PSY) catalyzes the highly regulated, frequently rate-limiting synthesis of the first biosynthetically formed carotene. While PSY constitutes a small gene family in most plant taxa, the Brassicaceae, including Arabidopsis (Arabidopsis thaliana), predominantly possess a single PSY gene. This monogenic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01262

    authors: Álvarez D,Voß B,Maass D,Wüst F,Schaub P,Beyer P,Welsch R

    更新日期:2016-12-01 00:00:00

  • Malate Decarboxylation by Kalanchoë daigremontiana Mitochondria and Its Role in Crassulacean Acid Metabolism.

    abstract::Mitochondria isolated from Kalanchoë daigremontiana, a Crassulacean acid metabolism plant, decarboxylate added malate to pyruvate at rates of up to 100 micromoles per hour per milligram original chlorophyll in the presence of ADP. Omitting ADP reduces this rate by approximately 50%. Antimycin A inhibits malate decarbo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.4.675

    authors: Day DA

    更新日期:1980-04-01 00:00:00

  • DNA free energy-based promoter prediction and comparative analysis of Arabidopsis and rice genomes.

    abstract::The cis-regulatory regions on DNA serve as binding sites for proteins such as transcription factors and RNA polymerase. The combinatorial interaction of these proteins plays a crucial role in transcription initiation, which is an important point of control in the regulation of gene expression. We present here an analy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.167809

    authors: Morey C,Mookherjee S,Rajasekaran G,Bansal M

    更新日期:2011-07-01 00:00:00

  • Root Exudates of Various Host Plants of Rhizobium leguminosarum Contain Different Sets of Inducers of Rhizobium Nodulation Genes.

    abstract::Rhizobium promoters involved in the formation of root nodules on leguminous plants are activated by flavonoids in plant root exudate. A series of Rhizobium strains which all contain the inducible Rhizobium leguminosarum nodA promoter fused to the Escherichia coli lacZ gene, and which differ only in the source of the r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.4.1298

    authors: Zaat SA,Wijffelman CA,Mulders IH,van Brussel AA,Lugtenberg BJ

    更新日期:1988-04-01 00:00:00

  • Method for overcoming the antiethylene effects of ag.

    abstract::A technique is described for eliminating the antiethylene effects of the Ag(+) ion in the intact pea plant (Pisum sativum). The technique is based on the ability of the ethylene mimic, acetylene, to negate the antiethylene effect of Ag(+), presumably through salt formation, and subsequently to induce the ethylene resp...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.4.616

    authors: Beyer EM

    更新日期:1978-10-01 00:00:00

  • Immunocytolocalization of polygalacturonase in ripening tomato fruit.

    abstract::Using tissue blotting and immunocytolocalization, we have investigated the appearance and accumulation of polygalacturonase (PG) during tomato (Lycopersicon esculentum Mill.) fruit ripening. Results show that PG first appears in the collumella region followed by sequential appearance in the exopericarp and endopericar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.1.17

    authors: Tieman DM,Handa AK

    更新日期:1989-05-01 00:00:00

  • Appearance of an Alternate Pathway Cyanide-resistant during Germination of Seeds of Cicer arietinum.

    abstract::The combined action of the inhibitors antimycin A and cyanide with benzohydroxamic acid indicates the presence of a cyanide-resistant pathway of respiration in chick pea (Cicer arietinum L.) seeds. The appearance of this pathway takes place during germination. During the first 12 hours of germination, the respiration ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.4.524

    authors: Burguillo Pde L,Nicolás G

    更新日期:1977-10-01 00:00:00

  • Starch-branching enzyme I-deficient mutation specifically affects the structure and properties of starch in rice endosperm.

    abstract::We have isolated a starch mutant that was deficient in starch-branching enzyme I (BEI) from the endosperm mutant stocks of rice (Oryza sativa) induced by the treatment of fertilized egg cells with N-methyl-N-nitrosourea. The deficiency of BEI in this mutant was controlled by a single recessive gene, tentatively design...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.021527

    authors: Satoh H,Nishi A,Yamashita K,Takemoto Y,Tanaka Y,Hosaka Y,Sakurai A,Fujita N,Nakamura Y

    更新日期:2003-11-01 00:00:00

  • Overexpression of RING Domain E3 Ligase ZmXerico1 Confers Drought Tolerance through Regulation of ABA Homeostasis.

    abstract::Drought stress is one of the main environmental problems encountered by crop growers. Reduction in arable land area and reduced water availability make it paramount to identify and develop strategies to allow crops to be more resilient in water-limiting environments. The plant hormone abscisic acid (ABA) plays an impo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01072

    authors: Brugière N,Zhang W,Xu Q,Scolaro EJ,Lu C,Kahsay RY,Kise R,Trecker L,Williams RW,Hakimi S,Niu X,Lafitte R,Habben JE

    更新日期:2017-11-01 00:00:00

  • Detecting mutants that have impaired photosynthesis by their increased level of fluorescence.

    abstract::A technique is described for detecting mutants of Chlamydomonas reinhardi that have impaired photosynthesis. It is based on the fact that when photosynthetic electron transport is blocked there may be an increase in the level of fluorescence similar to that obtained in the presence of an inhibitor of photosynthetic el...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.9.1284

    authors: Bennoun P,Levine RP

    更新日期:1967-09-01 00:00:00

  • Complementation of sugary-1 phenotype in rice endosperm with the wheat isoamylase1 gene supports a direct role for isoamylase1 in amylopectin biosynthesis.

    abstract::To examine the role of isoamylase1 (ISA1) in amylopectin biosynthesis in plants, a genomic DNA fragment from Aegilops tauschii was introduced into the ISA1-deficient rice (Oryza sativa) sugary-1 mutant line EM914, in which endosperm starch is completely replaced by phytoglycogen. A. tauschii is the D genome donor of w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.051359

    authors: Kubo A,Rahman S,Utsumi Y,Li Z,Mukai Y,Yamamoto M,Ugaki M,Harada K,Satoh H,Konik-Rose C,Morell M,Nakamura Y

    更新日期:2005-01-01 00:00:00

  • Synthesis of early heat shock proteins in young leaves of barley and sorghum.

    abstract::The in vivo synthesis of early heat-shock proteins in young leaves of barley (Hordeum vulgare L.) and sorghum (Sorghum bicolor L.) was studied by one- and two-dimensional electrophoresis. Analysis of whole leaf protein patterns demonstrated clearly the enhanced resolution of heat-shock proteins, especially those of lo...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.2.567

    authors: Clarke AK,Critchley C

    更新日期:1990-10-01 00:00:00

  • Adaptations of Photosynthetic Electron Transport, Carbon Assimilation, and Carbon Partitioning in Transgenic Nicotiana plumbaginifolia Plants to Changes in Nitrate Reductase Activity.

    abstract::Transgenic Nicotiana plumbaginifolia plants that express either a 5-fold increase or a 20-fold decrease in nitrate reductase (NR) activity were used to study the relationships between carbon and nitrogen metabolism in leaves. Under saturating irradiance the maximum rate of photosynthesis, per unit surface area, was de...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.1.171

    authors: Foyer CH,Lescure JC,Lefebvre C,Morot-Gaudry JF,Vincentz M,Vaucheret H

    更新日期:1994-01-01 00:00:00

  • Analysis of ethylene signal-transduction kinetics associated with seedling-growth response and chitinase induction in wild-type and mutant arabidopsis.

    abstract::Kinetic aspects of ethylene-mediated signal transduction leading to seedling-growth inhibition and chitinase induction in Arabidopsis were investigated by the introduction of defined mutations in components of these pathways. Dose-response analysis of wild-type responses indicated that the rate-limiting steps for seed...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.2.597

    authors: Chen QG,Bleecker AB

    更新日期:1995-06-01 00:00:00

  • Auxin Conjugation by Tobacco Mesophyll Protoplasts : Correlations between Auxin Cytotoxicity under Low Density Growth Conditions and Induction of Conjugation Processes at High Density.

    abstract::Auxin induction of the proliferation of Nicotiana tabacum (cv Xanthi) mesophyll protoplasts and of protoplast-derived cells was studied. The growth-promoting properties and cytotoxicities at high concentrations of IAA and naphthaleneacetic acid were strongly affected by cell density. The induction of growth by 2,4-dic...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.1.54

    authors: Caboche M,Aranda G,Poll AM,Huet JC,Leguay JJ

    更新日期:1984-05-01 00:00:00

  • Ketose reductase activity in developing maize endosperm.

    abstract::Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.3.830

    authors: Doehlert DC

    更新日期:1987-07-01 00:00:00

  • Inositol Metabolism in Plants. III. Conversion of Myo-inositol-2-H to Cell Wall Polysaccharides in Sycamore (Acer pseudoplatanus L.) Cell Culture.

    abstract::Prolonged growth of cell cultures of sycamore (Acer pseudoplatanus L.) on agar medium containing myo-inositol-2-(3)H resulted in incorporation of label predominately into uronosyl and pentosyl units of cell wall polysaccharides. Procedures normally used to distinguish between pectic substance and hemicellulose yielded...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.9.1489

    authors: Roberts RM,Loewus F

    更新日期:1966-11-01 00:00:00

  • Isolation of Sperms from the Pollen Tubes of Flowering Plants during Fertilization.

    abstract::Sperm cells have been isolated from pollen tubes growing in style segments of the dicotlyledon Rhododendron macgregoriae and the monocotyledon Gladiolus gandavensis by the in vivo/in vitro method at various stages of fertilization. Pollen tubes emerged from the cut end of the style into agar medium, and more than 95% ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.87.3.647

    authors: Shivanna KR,Xu H,Taylor P,Knox RB

    更新日期:1988-07-01 00:00:00

  • Identification of a new glucosinolate-rich cell type in Arabidopsis flower stalk.

    abstract::Distribution of K, Ca, Cl, S, and P in freeze-dried sections of Arabidopsis flower stalk was analyzed by energy dispersive x-ray imaging. Concentrations of these elements in different cell types were quantified by microanalysis of single-cell samples and phloem exudates. Results showed a differential pattern of distri...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.2.599

    authors: Koroleva OA,Davies A,Deeken R,Thorpe MR,Tomos AD,Hedrich R

    更新日期:2000-10-01 00:00:00

  • High-resolution mapping of a fruit firmness-related quantitative trait locus in tomato reveals epistatic interactions associated with a complex combinatorial locus.

    abstract::Fruit firmness in tomato (Solanum lycopersicum) is determined by a number of factors including cell wall structure, turgor, and cuticle properties. Firmness is a complex polygenic trait involving the coregulation of many genes and has proved especially challenging to unravel. In this study, a quantitative trait locus ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.200634

    authors: Chapman NH,Bonnet J,Grivet L,Lynn J,Graham N,Smith R,Sun G,Walley PG,Poole M,Causse M,King GJ,Baxter C,Seymour GB

    更新日期:2012-08-01 00:00:00

  • Cytokinin-controlled Indoleacetic Acid Oxidase Isoenzymes in Tobacco Callus Cultures.

    abstract::Indoleacetic acid oxidase in tobacco callus tissues (Nicotiana tabacum L., cultivar White Gold) was resolved into seven anionic isoenzymes by polyacrylamide gel disc electrophoresis. Different concentrations of kinetin and zeatin in the presence of indoleacetic acid affected the level of this enzyme, particularly two ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.47.2.181

    authors: Lee TT

    更新日期:1971-02-01 00:00:00

  • Growth-induced Water Potentials in Plant Cells and Tissues.

    abstract::A physical analysis of water movement through elongating soybean (Glycine max L. Merr.) hypocotyls was made to determine why significant water potentials persist in growing tissues even though the external water potentials were zero and transpiration is virtually zero. The analysis was based on a water transport theor...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.3.423

    authors: Molz FJ

    更新日期:1978-09-01 00:00:00

  • Characterization of Sucrolysis via the Uridine Diphosphate and Pyrophosphate-Dependent Sucrose Synthase Pathway.

    abstract::The breakdown of sucrose to feed both hexoses into glycolytic carbon flow can occur by the sucrose synthase pathway. This uridine diphosphate (UDP) and pyrophosphate (PPi)-dependent pathway was biochemically characterized using soluble extracts from several plants. The sucrolysis process required the simultaneous pres...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.2.635

    authors: Xu DP,Sung SJ,Loboda T,Kormanik PP,Black CC

    更新日期:1989-06-01 00:00:00

  • Effect of plant hormones on sucrose uptake by sugar beet root tissue discs.

    abstract::Abscisic acid (ABA), auxins, cytokinins, gibberellic acid, alone or in combination were tested for their effects on short-term sucrose uptake in sugar beet (Beta vulgaris cv USH-20) roots. The effect of ABA on active sucrose uptake varied from no effect to the more generally observed 1.4-to 3.0-fold stimulation. A rac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.4.951

    authors: Saftner RA,Wyse RE

    更新日期:1984-04-01 00:00:00

  • Effect of Benzyladenine Treatment Duration on delta-Aminolevulinic Acid Accumulation in the Dark, Chlorophyll Lag Phase Abolition, and Long-Term Chlorophyll Production in Excised Cotyledons of Dark-Grown Cucumber Seedlings.

    abstract::Cotyledons excised from dark-grown cucumber (Cucumis sativus L. cv. Aonagajibai) seedlings were incubated in the dark with the cytokinin benzyladenine for different time periods. Then, various greening parameters were examined, including protochlorophyll(ide) to chlorophyll(ide) photoconversion and delta-aminolevulini...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.3.663

    authors: Lew R,Tsuji H

    更新日期:1982-03-01 00:00:00

  • Differences in water relations and physiological characteristics in leaves of wheat associated with leaf position on the plant.

    abstract::The seasonal change in leaf water potential and its components, stomatal resistance, specific leaf weight, photosynthesis rate, the activities of ribulose-1,5-bisphosphate carboxylase and nitrate reductase, and soluble proteins were measured in flag leaves (ninth from base in position), seventh and fifth leaves of whe...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.74.4.1041

    authors: Aggarwal PK,Sinha SK

    更新日期:1984-04-01 00:00:00