Identification of a new glucosinolate-rich cell type in Arabidopsis flower stalk.

abstract：:The kinetics of l-aspartate transport into pea chloroplasts was studied in the presence and absence of transport inhibitors to determine whether multiple aspartate carriers exist. Transport was measured by the silicone oil centrifugation technique. Reciprocal plots of concentration-dependent transport rates were bipha...

journal_title：Plant physiology

authors： Werner-Washburne M,Keegstra K

更新日期：1985-06-01 00:00:00

abstract：:A fructokinase (EC 2.7.1.4) was obtained from pea (Pisum sativum L.) seeds. This enzyme, termed fructokinase (fraction IV), was specific for fructose as substrate and had little activity with glucose or mannose. Excess fructose inhibited the enzyme at the optimum pH (8.2) but not at pH 6.6. MgATP was inhibitory at pH ...

journal_title：Plant physiology

authors： Turner JF,Harrison DD,Copeland L

更新日期：1977-11-01 00:00:00

abstract：:We have analyzed the possibility that chemical signaling does not entirely account for the effect of water deficit on the maize (Zea mays L.) leaf elongation rate (LER) under high evaporative demand. We followed time courses of LER (0.2-h interval) and spatial distribution of elongation rate in leaves of either water-...

journal_title：Plant physiology

authors： Salah H,Tardieu F

更新日期：1997-07-01 00:00:00

abstract：:The effects of phosphorus deficiency on carbohydrate accumulation and utilization in 34-day-old soybean (Glycine max L. Merr.) plants were characterized over a diurnal cycle to evaluate the mechanisms by which phosphorus deficiency restricts plant growth. Phosphorus deficiency decreased the net CO(2) exchange rate thr...

journal_title：Plant physiology

authors： Qiu J,Israel DW

更新日期：1992-01-01 00:00:00

abstract：:We have isolated the plasma membrane H+-ATPase in a phosphorylated form from spinach (Spinacia oleracea L.) leaf tissue incubated with fusicoccin, a fungal toxin that induces irreversible binding of 14-3-3 protein to the C terminus of the H+-ATPase, thus activating H+ pumping. We have identified threonine-948, the sec...

journal_title：Plant physiology

authors： Olsson A,Svennelid F,Ek B,Sommarin M,Larsson C

更新日期：1998-10-01 00:00:00

abstract：:An important question concerning the role of carboxyarabinitol 1-phosphate (CA1P) metabolism in the light-dependent regulation of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity is the extent to which CA1P is bound to Rubisco in vivo. We report here the development of an extraction procedure using a...

journal_title：Plant physiology

authors： Moore B,Seemann JR

更新日期：1994-06-01 00:00:00

abstract：:Auxins and cytokinins support cell division in tissue and cell cultures. In cytokinin-independent pear (Pyrus communis) cells, omission of 2,4-dichlorophenoxyacetic acid (2,4-D) from the medium for two successive transfers leads to rapid cell lysis, unless the osmolarity is raised to 0.4 molar with mannitol. Use of th...

journal_title：Plant physiology

authors： Pech JC,Romani RJ

更新日期：1979-11-01 00:00:00

abstract：:We have identified a wall hydrolytic enzyme from Trichoderma reesei with potent ability to induce extension of heat-inactivated type I cell walls. It is a small (23-kD) endo-1,4-beta-glucanase (Cel12A) belonging to glycoside hydrolase family 12. Extension of heat-inactivated walls from cucumber (Cucumis sativus cv Bur...

journal_title：Plant physiology

authors： Yuan S,Wu Y,Cosgrove DJ

更新日期：2001-09-01 00:00:00

abstract：:Our results show that the phosphate ion is a nonlinear competitive inhibitor of Pinus pinea isocitrate lyase. In addition, this compound induces a sigmoidal response of the enzyme, which usually exhibits standard Michaelis-Menten kinetics. This peculiar behavior of P. pinea isocitrate lyase could be explained by a dim...

journal_title：Plant physiology

authors： Ranaldi F,Vanni P,Giachetti E

更新日期：2000-11-01 00:00:00

abstract：:Adenosine kinase was partially purified from wheat germ. This enzyme preparation, which was devoid of adenine phosphoribosyltransferase and nearly free of adenosine deaminase but contained adenylate kinase, rapidly phosphorylated adenosine and a cytokinin, N(6)-(delta(2)-isopentenyl)adenosine. Electrophoretic analysis...

journal_title：Plant physiology

authors： Chen CM,Eckert RL

更新日期：1977-03-01 00:00:00

abstract：:The first compound in the series of reactions leading to the ester conjugates of indole-3-acetic acid (IAA) in kernels of Zea mays sweet corn is the acyl alkyl acetal, 1-O-indol-3-ylacetyl-beta-D-glucose (1-O-IAGlu). The enzyme catalyzing the synthesis of this compound is UDP-glucose:indol-3-ylacetate glucosyl-transf...

journal_title：Plant physiology

authors： Kowalczyk S,Bandurski RS

更新日期：1990-01-01 00:00:00

abstract：:Opaque or nonvitreous phenotypes relate to the seed architecture of maize (Zea mays) and are linked to loci that control the accumulation and proper deposition of storage proteins, called zeins, into specialized organelles in the endosperm, called protein bodies. However, in the absence of null mutants of each type of...

journal_title：Plant physiology

authors： Wu Y,Messing J

更新日期：2010-05-01 00:00:00

abstract：:Growth response of the seagrass Halophila engelmanni Aschers. was studied using defined culture techniques. When added to the rhizosphere, nutrient-seawater medium supported vigorous plant growth, if substantial Cu (10 times that in modified Hoagland solution) plus organic N were present. H(2)S up to 0.2 millimolar in...

journal_title：Plant physiology

authors： Pulich WM

更新日期：1983-04-01 00:00:00

abstract：:The lv-1 mutant of pea (Pisum sativum L.) is deficient in responses regulated by phytochrome B (phyB) in other species but has normal levels of spectrally active phyB. We have characterized three further lv mutants (lv-2, lv-3, and lv-4), which are all elongated under red (R) and white light but are indistinguishable ...

journal_title：Plant physiology

authors： Weller JL,Nagatani A,Kendrick RE,Murfet IC,Reid JB

更新日期：1995-06-01 00:00:00

abstract：:Mean inhibition of net photosynthesis in autotrophic tobacco callus by 21 and 40% O(2) was 30 and 47%, respectively, similar to intact leaves. Increasing CO(2) concentrations (500-2000 microliters per liter) produced a steady decline in percent inhibition at both O(2) concentrations, indicating that O(2) inhibition re...

journal_title：Plant physiology

authors： McHale NA,Zelitch I,Peterson RB

更新日期：1987-08-01 00:00:00

abstract：:Activity of the enzyme ADPglucose pyrophosphorylase is known to be reduced in maize (Zea mays L.) endosperm mutants at two independent loci, Shrunken-2 (Sh(2)) and Brittle-2 (Bt(2)). Spinach leaf ADPglucose pyrophosphorylase has previously been shown to comprise two subunits of 51 and 54 kilodaltons. Anti-bodies raise...

journal_title：Plant physiology

authors： Preiss J,Danner S,Summers PS,Morell M,Barton CR,Yang L,Nieder M

更新日期：1990-04-01 00:00:00

abstract：:Cobalt was shown to be essential for the symbiotic growth of Azolla filiculoides and Anabacna azollac in the absence of fixed nitrogen. Addition of 0.01 mug/liter cobalt resulted in large increases in yield, chlorophyll content and nitrogen fixation as compared to control cultures without cobalt. Cobalt was not requir...

journal_title：Plant physiology

authors： Johnson GV,Mayeux PA,Evans HJ

更新日期：1966-05-01 00:00:00

abstract：:Lotus pedunculatus L., Medicago sativa L., Macroptilium atropurpureum, Glycine max, and Trifolium repens L. were grown in a N-free medium and inoculated with one of ten Rhizobium strains. Dry matter, N content, and delta(15)N values were determined for various plant parts.Nodules, with the exception of those from luce...

journal_title：Plant physiology

authors： Steele KW,Bonish PM,Daniel RM,O'hara GW

更新日期：1983-08-01 00:00:00

abstract：:The study aimed to test the hypothesis that ammonia production by Rhizobium bacteroids provides not only a source of nitrogen for growth but has a central regulatory role in maintaining the metabolic activity and functional integrity of the legume nodule. Production of ammonia in intact, attached nodules was interrupt...

journal_title：Plant physiology

authors： Atkins CA,Pate JS,Shelp BJ

更新日期：1984-11-01 00:00:00

abstract：:An enzyme activity, found for the first time in plants, mainly located in the 22,000g supernatant of the crude extract of sprout apices of Helianthus tuberosus L. cv OB1 tubers, is able in vitro to covalently bind polyamines to endogenous substrates of different molecular weights. The major assay parameters, such as p...

journal_title：Plant physiology

authors： Serafini-Fracassini D,Del Duca S,D'Orazi D

更新日期：1988-07-01 00:00:00

abstract：:Extracts of light-grown Cucumis sativus L. seedlings catalyzed the oxidation of indole-3-acetaldehyde to indole-3-acetic acid. No added cofactors were required. Inhibitor studies indicated that the enzyme is a metalloflavoprotein. While indole-3-aldehyde, benzaldehyde, and phenylacetaldehyde partially inhibited the ox...

journal_title：Plant physiology

authors： Bower PJ,Brown HM,Purves WK

更新日期：1978-01-01 00:00:00

abstract：:An ATPase preparation, presumed to be associated with plasma membrane due to the coincidence on isopycnic gradients of cellulase and beta-glucan synthetase at high substrate, has been isolated from the epidermal and mesophyll of tobacco leaf. The ATPase from both tissues was found to prefer ATP over other nucleotides....

journal_title：Plant physiology

authors： Lurie S

更新日期：1979-05-01 00:00:00

abstract：:A comparative study was made of the effects of auxin (alpha-naphthalene acetic acid), kinetin (6-furfurylaminopurine) and a mixture of auxin and kinetin applied in vivo on synthesis of RNA and protein and the distribution of such synthesis amongst the subcellular fractions of sections of endocarp from Kentucky Wonder ...

journal_title：Plant physiology

authors： Sacher JA

更新日期：1967-10-01 00:00:00

abstract：:Measurements of metabolic fluxes in whole embryos and isolated plastids have revealed major changes in the pathways of carbon utilization during cotyledon filling by oilseed rape (Brassica napus L.) embryos. In the early cotyledon stage (stage A), embryos used sucrose (Suc) predominantly for starch synthesis. Plastids...

journal_title：Plant physiology

authors： Eastmond PJ,Rawsthorne S

更新日期：2000-03-01 00:00:00

abstract：:To function, the catalytic sites of Rubisco (EC 4.1.1.39) need to be activated by the reversible carbamylation of a lysine residue within the sites followed by rapid binding of magnesium. The activation of Rubisco in vivo requires the presence of the regulatory protein Rubisco activase. This enzyme is thought to aid t...

journal_title：Plant physiology

authors： von Caemmerer S,Hendrickson L,Quinn V,Vella N,Millgate AG,Furbank RT

更新日期：2005-02-01 00:00:00

abstract：:The effect of initial oxygen concentration on the rate and extent of oxidation of the respiratory chain carriers of anaerobic mitochondria from mung bean (Phaseolus aureus) seedlings was examined. The substrate was succinate, with malonate added to give malonate to succinate ratios of 6 to 12, thereby minimizing the f...

journal_title：Plant physiology

authors： Storey BT,Bahr JT

更新日期：1972-07-01 00:00:00

abstract：:Interactions between putrescine and membrane function were examined with the use of a recently developed microelectrode system that enables us simultaneously to quantify membrane potentials and net K(+) fluxes associated with individual cells at the root surface of an intact corn (Zea mays L.) seedling. In contrast to...

journal_title：Plant physiology

authors： Ditomaso JM,Shaff JE,Kochian LV

更新日期：1989-07-01 00:00:00

abstract：:Mg-chelatase catalyzes the first step unique to the chlorophyll branch of tetrapyrrole biosynthesis, namely the insertion of Mg into protoporphyrin IX (Proto). Mg-chelatase was assayed in intact chloroplasts from semi-green cucumber (Cucumis sativus, cv Sumter) cotyledons. In the presence of Proto and MgATP, enzyme ac...

journal_title：Plant physiology

authors： Walker CJ,Weinstein JD

更新日期：1991-04-01 00:00:00

abstract：:Synechocystis PCC 6803 contains two types of glutathione peroxidase-like proteins (GPX-1 and GPX-2) that utilize NADPH but not reduced glutathione and unsaturated fatty acid hydroperoxides or alkyl hydroperoxides. The steady-state transcript level of gpx-1 gradually increased under oxidative stress conditions imposed ...

journal_title：Plant physiology

authors： Gaber A,Yoshimura K,Tamoi M,Takeda T,Nakano Y,Shigeoka S

更新日期：2004-09-01 00:00:00

abstract：:We have described the modulation of four auxin-regulated genes during the growth cycle of suspension-cultured tobacco (Nicotiana tabacum [L.] var White Burley) cells. The genes were transiently expressed 2 to 8 h after transfer of stationary phase cells to fresh medium, during the transition from the quiescent phase o...

journal_title：Plant physiology

authors： Boot K,Van Der Zaal BJ,Velterop J,Quint A,Mennes AM,Hooykaas P,Libbenga KR

更新日期：1993-06-01 00:00:00