Synthesis of early heat shock proteins in young leaves of barley and sorghum.

Abstract:

:The in vivo synthesis of early heat-shock proteins in young leaves of barley (Hordeum vulgare L.) and sorghum (Sorghum bicolor L.) was studied by one- and two-dimensional electrophoresis. Analysis of whole leaf protein patterns demonstrated clearly the enhanced resolution of heat-shock proteins, especially those of low molecular weight, when separated by two-dimensional electrophoresis. Comparison between the two cereals showed that a greater number and diversity of heat-shock proteins were induced in the subtropical C(4) (sorghum) species compared to the temperate C(3) (barley) species. Fractionation of whole leaf proteins into soluble and membrane fractions showed the majority of heat-shock proteins to be associated with the soluble fraction in both sorghum and barley. However, several low molecular mass (17-24 kilodalton) heat-shock proteins were clearly identified in the membrane fractions, indicating a likely association with thylakoid membranes in vivo during the early stages of a heat-shock response in both species.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Clarke AK,Critchley C

doi

10.1104/pp.94.2.567

subject

Has Abstract

pub_date

1990-10-01 00:00:00

pages

567-76

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

94

pub_type

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