Abstract:
:The in vivo synthesis of early heat-shock proteins in young leaves of barley (Hordeum vulgare L.) and sorghum (Sorghum bicolor L.) was studied by one- and two-dimensional electrophoresis. Analysis of whole leaf protein patterns demonstrated clearly the enhanced resolution of heat-shock proteins, especially those of low molecular weight, when separated by two-dimensional electrophoresis. Comparison between the two cereals showed that a greater number and diversity of heat-shock proteins were induced in the subtropical C(4) (sorghum) species compared to the temperate C(3) (barley) species. Fractionation of whole leaf proteins into soluble and membrane fractions showed the majority of heat-shock proteins to be associated with the soluble fraction in both sorghum and barley. However, several low molecular mass (17-24 kilodalton) heat-shock proteins were clearly identified in the membrane fractions, indicating a likely association with thylakoid membranes in vivo during the early stages of a heat-shock response in both species.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Clarke AK,Critchley Cdoi
10.1104/pp.94.2.567subject
Has Abstractpub_date
1990-10-01 00:00:00pages
567-76issue
2eissn
0032-0889issn
1532-2548journal_volume
94pub_type
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