Phosphatidylinositol specific phospholipase C of plant stems : membrane associated activity concentrated in plasma membranes.

Abstract:

:A phosphatidylinositol-specific phospholipase C of plant stems (EC 3.1.4.10) assayed at pH 6.6 and at 30 degrees C cleaved phosphatidylinositol such that more than 85% of the product was inositol-1-phosphate. Other phospholipids were cleaved 5 to 10% or less under these conditions. The phospholipase had both a soluble and a membrane-associated form. The soluble activity accounted for approximately 85 to 90% of the activity and 15% was associated with membranes. The membrane-associated activity was most concentrated in the plasma membranes of hypocotyl segments of both soybean (Glycine max) and bushbean (Phaseolus vulgaris). The plasma membrane location was verified by analysis of highly purified plasma membranes prepared both by aqueous two-phase partitioning and by preparative free-flow electrophoresis and from the quantitation of the activity in all major cell fractions. Internal membranes also contained phospholipase C activity but at specific activity levels of about 0.1 those present in plasma membranes. Golgi apparatus-enriched fractions from which plasma membrane contaminants were removed by two-phase partition contained the activity at specific activity levels 0.2 those of plasma membrane. Both the soluble and the membrane-associated activity was stimulated by calcium but not by calmodulin, either alone or in the presence of calcium.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Pfaffmann H,Hartmann E,Brightman AO,Morré DJ

doi

10.1104/pp.85.4.1151

subject

Has Abstract

pub_date

1987-12-01 00:00:00

pages

1151-5

issue

4

eissn

0032-0889

issn

1532-2548

journal_volume

85

pub_type

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