Abstract:
:A phosphatidylinositol-specific phospholipase C of plant stems (EC 3.1.4.10) assayed at pH 6.6 and at 30 degrees C cleaved phosphatidylinositol such that more than 85% of the product was inositol-1-phosphate. Other phospholipids were cleaved 5 to 10% or less under these conditions. The phospholipase had both a soluble and a membrane-associated form. The soluble activity accounted for approximately 85 to 90% of the activity and 15% was associated with membranes. The membrane-associated activity was most concentrated in the plasma membranes of hypocotyl segments of both soybean (Glycine max) and bushbean (Phaseolus vulgaris). The plasma membrane location was verified by analysis of highly purified plasma membranes prepared both by aqueous two-phase partitioning and by preparative free-flow electrophoresis and from the quantitation of the activity in all major cell fractions. Internal membranes also contained phospholipase C activity but at specific activity levels of about 0.1 those present in plasma membranes. Golgi apparatus-enriched fractions from which plasma membrane contaminants were removed by two-phase partition contained the activity at specific activity levels 0.2 those of plasma membrane. Both the soluble and the membrane-associated activity was stimulated by calcium but not by calmodulin, either alone or in the presence of calcium.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Pfaffmann H,Hartmann E,Brightman AO,Morré DJdoi
10.1104/pp.85.4.1151subject
Has Abstractpub_date
1987-12-01 00:00:00pages
1151-5issue
4eissn
0032-0889issn
1532-2548journal_volume
85pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The temperature response of net CO(2) assimilation rate (A), the rate of whole-chain electron transport, the activity and activation state of Rubisco, and the pool sizes of ribulose-1,5-bisphosphate (RuBP) and 3-phosphoglyceric acid (PGA) were assessed in sweet potato (Ipomoea batatas) grown under greenhouse condition...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.066233
更新日期:2005-10-01 00:00:00
abstract::A previously isolated rice (Oryza sativa) rbcS gene was further characterized. This analysis revealed specific sequences in the 5' regulatory region of the rice rbcS gene that are conserved in rbcS genes of other monocotyledonous species. In transgenic rice plants, we examined the expression of the beta-glucuronidase ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.3.991
更新日期:1993-07-01 00:00:00
abstract::Low-oxygen stress imposed by field waterlogging is a serious impediment to plant germination and growth. Plants respond to waterlogging with a complex set of physiological responses regulated at the transcriptional, cellular, and tissue levels. The Arabidopsis (Arabidopsis thaliana) NAC domain-containing gene ANAC102 ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.131912
更新日期:2009-04-01 00:00:00
abstract::Extracts of maize leaf sheath tissue deaminate both l-phenylalanine and l-tyrosine. The activities with both substrates are enhanced by treating the plant with gibberellic acid. Both activities decrease rapidly at the same rate when tissue is incubated in a moist atmosphere, and this decrease can be slowed by treatmen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.50.4.480
更新日期:1972-10-01 00:00:00
abstract::A light-induced shrinkage of chloroplasts in vivo could be detected with chloroplasts isolated within 2 minutes of harvesting pea plants. As determined both by packed volume and Coulter counter, the mean volume of chloroplasts from plants in the dark was 39 mu(3), whereas it was 31 mu(3) for chloroplasts from plants i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.5.781
更新日期:1968-05-01 00:00:00
abstract::Many eukaryotes have from one to three heat shock factors (Hsfs), but plants have more than 20 Hsfs, designated class A, B, and C. Class A Hsfs are activators of transcription, but details of the roles of individual Hsfs have not been fully characterized. We show here that Arabidopsis (Arabidopsis thaliana) HsfB1 and ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.179036
更新日期:2011-11-01 00:00:00
abstract::Mean inhibition of net photosynthesis in autotrophic tobacco callus by 21 and 40% O(2) was 30 and 47%, respectively, similar to intact leaves. Increasing CO(2) concentrations (500-2000 microliters per liter) produced a steady decline in percent inhibition at both O(2) concentrations, indicating that O(2) inhibition re...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.4.1055
更新日期:1987-08-01 00:00:00
abstract::Aspartate aminotransferase (AAT), a key enzyme in the biosynthesis of aspartate and asparagine, occurs as two forms in alfalfa (Medicago sativa L.), AAT-1 and AAT-2. Both forms were purified to near homogeneity, and high titer polyclonal antibodies produced to the native proteins. Alfalfa AAT-1 was purified from root ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.2.603
更新日期:1990-06-01 00:00:00
abstract::The unicellular algae Chlorella sorokiniana was used as a model system to investigate the interaction of ozone with plant cell membranes. Ozone induces K(+) leakage from Chlorella sorokiniana similar to the electrolytic loss observed from many higher plants under stressful conditions. The kinetics of this leakage indi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.56.6.723
更新日期:1975-12-01 00:00:00
abstract::The surface tension (γ) of xylem sap plays a key role in stabilizing air-water interfaces at the pits between water- and gas-filled conduits to avoid air seeding at low water potentials. We studied seasonal changes in xylem sap γ in Picea abies and Pinus mugo growing at the alpine timberline. We analyzed their vulnera...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01053
更新日期:2017-11-01 00:00:00
abstract::A two-dimensional gel electrophoresis system that combines a cationic polyacrylamide gel electrophoresis at pH near neutrality with sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to analyze the spectrum of basic polypeptides that accumulate in bean (Phaseolus vulgaris) abscission zones after treatm...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.3.955
更新日期:1992-03-01 00:00:00
abstract::Glyphosate, the most commonly used herbicide in the world, controls a wide range of plant species, mainly because plants have little capacity to metabolize (detoxify) glyphosate. Massive glyphosate use has led to world-wide evolution of glyphosate-resistant (GR) weed species, including the economically damaging grass ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.00979
更新日期:2019-12-01 00:00:00
abstract::Analysis of Sorghum bicolor bloomless (bm) mutants with altered epicuticular wax (EW) structure uncovered a mutation affecting both EW and cuticle deposition. The cuticle of mutant bm-22 was about 60% thinner and approximately one-fifth the weight of the wild-type parent P954035 (WT-P954035) cuticles. Reduced cuticle ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.4.1239
更新日期:1994-08-01 00:00:00
abstract::Circular RNAs (circRNAs) play roles in various biological processes, but their functions in the rice (Oryza sativa) response to Magnaporthe oryzae remain elusive. Here, we demonstrate that circRNAs are involved in the rice-M. oryzae interaction using comparative circRNA-sequencing and transgenic approaches. We identif...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.00716
更新日期:2020-01-01 00:00:00
abstract::Photosystem I electron transport activity has been found to be considerably higher in a decaploid tall fescue (Festuca arundinacea Schreb.) genotype as compared to a common hexaploid genotype. The decaploid genotype also displayed a higher photosystem whole chain (Photosystem II plus Photosystem I uncoupled) activity,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.68.5.1110
更新日期:1981-11-01 00:00:00
abstract::The induction of phototropism in etiolated (dark-grown) seedlings exposed to an unidirectional pulse or extended irradiation with low fluence rate blue light (BL) requires the action of the phototropin (nph1) BL receptor. Although cryptochromes and phytochromes are not required for phototropic induction, these photore...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.2.826
更新日期:2001-06-01 00:00:00
abstract::A depolarization-activated K(+) channel capable of carrying the large K(+) currents that flow from shrinking cells during movements of Samanea saman leaflets has been described in the plasmalemma of Samanea motor cell protoplasts (N Moran et al [1988] Plant Physiol 88:643-648). We now characterize this channel in grea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.2.424
更新日期:1990-10-01 00:00:00
abstract::The development of photochemical activity and carbon assimilation in light-grown cucumber (Cucumis sativus L. cv Natsusairaku) leaves was studied to determine the pattern of acquisition and its relationship to leaf growth and expansion. Measurements of chlorophyll a fluorescence showed that leaves acquire photochemica...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.3.1078
更新日期:1990-07-01 00:00:00
abstract::Microsomal membranes from suspension-cultured Catharanthus roseus cells possess an enzymic activity that catalyzes the ATP-dependent phosphorylation of phosphatidic acid (PA) to form diacylglycerol pyrophosphate (H. Behrbohm, J.B. Wissing [1993] FEBS Lett 315: 95-99). This enzyme activity, PA kinase, was purified and ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.4.1243
更新日期:1993-08-01 00:00:00
abstract::A chimeric gene containing the patatin promoter and the transit-peptide region of the small-subunit carboxylase gene was utilized to direct expression of Escherichia coli glycogen synthase (glgA) to potato (Solanum tuberosum) tuber amyloplasts. Expression of the glgA gene product in tuber amyloplasts was between 0.007...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.4.1159
更新日期:1994-04-01 00:00:00
abstract::The Arabidopsis genome contains 14 genes encoding the serine protease DegP. Products of four of these genes are located in the chloroplast: three in the thylakoid lumen and one on the stromal side of the membrane. We expressed the gene encoding DegP1 as a His-tagged fusion protein in Escherichia coli, purified the pro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.007922
更新日期:2002-10-01 00:00:00
abstract::Bean (Phaseolus vulgaris L.) seeds contain a putative plant defense protein that inhibits insect and mammalian but not plant alpha-amylases. We recently (J Moreno, MJ Chrispeels [1989] Proc Natl Acad Sci USA 86:7885-7889) presented strong circumstantial evidence that this alpha-amylase inhibitor (alphaAI) is encoded b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.2.805
更新日期:1990-06-01 00:00:00
abstract::The effect of abscisic acid (ABA) on the exudation process in excised sunflower root was investigated. A promotion of both ion flux and volume exudation rate was observed. Cutting off the external supply of ions to the roots markedly increased the magnitude of the ABA effect. The promotive effect of ABA on exudation r...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.3.537
更新日期:1980-03-01 00:00:00
abstract::Sporopollenin is the major component of the outer pollen wall (exine). Fatty acid derivatives and phenolics are thought to be its monomeric building blocks, but the precise structure, biosynthetic route, and genetics of sporopollenin are poorly understood. Based on a phenotypic mutant screen in Arabidopsis (Arabidopsi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.144469
更新日期:2009-10-01 00:00:00
abstract::The rate of (14)C-leucine and (3)H-uracil incorporation by tobacco cells (Nicotiana tabaccum var. Samsun N.N.) in suspension culture was simultaneously decreased by the addition of kinetin at concentrations above 2.5 x 10(-5)m. Ribosomal RNA was the first RNA species affected by kinetin. The purine derivatives, adenin...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.47.3.400
更新日期:1971-03-01 00:00:00
abstract::Blue light is known to induce suppression of stem elongation. To avoid the complication of blue-light-induced transformation of phytochrome we have adapted the procedure of measuring blue-light-induced suppression of stem elongation in Pisum sativum L. var Alaska grown under continuous red light. The resulting fluence...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.89.2.544
更新日期:1989-02-01 00:00:00
abstract::To study the role of the plastidial alpha-glucan phosphorylase in starch metabolism in the leaves of Arabidopsis, two independent mutant lines containing T-DNA insertions within the phosphorylase gene were identified. Both insertions eliminate the activity of the plastidial alpha-glucan phosphorylase. Measurement of o...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.032631
更新日期:2004-06-01 00:00:00
abstract::The herbicide Sandoz 6706 (4-chloro-5-(dimethylamino)-2-alpha,alpha,alpha, (trifluoro-m-tolyl)-3(2H)-pyridazinone), when applied as a preplant soil treatment at a concentration of 0.05 mug/g reduced the content of beta-carotene and chlorophylls in 21-day-old wheat seedlings (Triticum aestivum L.) by 55% and 29%, respe...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.6.916
更新日期:1974-12-01 00:00:00
abstract::The trafficking and function of cell surface proteins in eukaryotic cells may require association with detergent-resistant sphingolipid- and sterol-rich membrane domains. The aim of this work was to obtain evidence for lipid domain phenomena in plant membranes. A protocol to prepare Triton X-100 detergent-resistant me...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.053041
更新日期:2005-01-01 00:00:00
abstract::As structural and functional genomics efforts provide the biological community with ever-broadening sets of interrelated data, the need to explore such complex information for subtle relationships expands. We present wCLUTO, a Web-enabled version of the stand-alone application CLUTO, designed to apply clustering metho...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.024885
更新日期:2003-10-01 00:00:00