circRNAs Are Involved in the Rice-Magnaporthe oryzae Interaction.

Abstract:

:Circular RNAs (circRNAs) play roles in various biological processes, but their functions in the rice (Oryza sativa) response to Magnaporthe oryzae remain elusive. Here, we demonstrate that circRNAs are involved in the rice-M. oryzae interaction using comparative circRNA-sequencing and transgenic approaches. We identified 2932 high-confidence circRNAs from young leaves of the blast-resistant accession International Rice Blast Line Pyricularia-Kanto51-m-Tsuyuake (IR25) and the blast-susceptible accession Lijiangxin Tuan Heigu (LTH) under Moryzae-infected or uninfected conditions; 636 were detected specifically upon Moryzae infection. The circRNAs in IR25 were significantly more diverse than those in LTH, especially under M. oryzae infection. Particularly, the number of circRNAs generated per parent gene was much higher in IR25 than in LTH and increased in IR25 but decreased in LTH upon M. oryzae infection. The higher diversity of circRNAs in IR25 was further associated with more frequent 3' and 5' alternative back-splicing and usage of complex splice sites. Moreover, a subset of circRNAs was differentially responsive to Moryzae in IR25 and LTH. We further confirmed that circR5g05160 promotes rice immunity against Moryzae Therefore, our data indicate that circRNA diversity is associated with different responses to Moryzae infection in rice and provide a starting point to investigate a new layer of regulation in the rice-Moryzae interaction.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Fan J,Quan W,Li GB,Hu XH,Wang Q,Wang H,Li XP,Luo X,Feng Q,Hu ZJ,Feng H,Pu M,Zhao JQ,Huang YY,Li Y,Zhang Y,Wang WM

doi

10.1104/pp.19.00716

subject

Has Abstract

pub_date

2020-01-01 00:00:00

pages

272-286

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.19.00716

journal_volume

182

pub_type

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