Abstract:
:Phenylpropanoid biosynthesis in plants engenders myriad phenolics with diverse biological functions. Phenylalanine ammonia-lyase (PAL) is the first committed enzyme in the pathway, directing primary metabolic flux into a phenylpropanoid branch. Previously, we demonstrated that the Arabidopsis (Arabidopsis thaliana) Kelch domain-containing F-box proteins, AtKFB01, AtKFB20, and AtKFB50, function as the negative regulators controlling phenylpropanoid biosynthesis via mediating PAL's ubiquitination and subsequent degradation. Here, we reveal that Arabidopsis KFB39, a close homolog of AtKFB50, also interacts physically with PAL isozymes and modulates PAL stability and activity. Disturbing the expression of KFB39 reciprocally affects the accumulation/deposition of a set of phenylpropanoid end products, suggesting that KFB39 is an additional posttranslational regulator responsible for the turnover of PAL and negatively controlling phenylpropanoid biosynthesis. Furthermore, we discover that exposure of Arabidopsis to ultraviolet (UV)-B radiation suppresses the expression of all four KFB genes while inducing the transcription of PAL isogenes; these data suggest that Arabidopsis consolidates both transcriptional and posttranslational regulation mechanisms to maximize its responses to UV light stress. Simultaneous down-regulation of all four identified KFBs significantly enhances the production of (poly)phenols and the plant's tolerance to UV irradiation. This study offers a biotechnological approach for engineering the production of useful phenolic chemicals and for increasing a plant's resistance to environmental stress.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Zhang X,Gou M,Guo C,Yang H,Liu CJdoi
10.1104/pp.114.249136subject
Has Abstractpub_date
2015-02-01 00:00:00pages
337-50issue
2eissn
0032-0889issn
1532-2548pii
pp.114.249136journal_volume
167pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The effect of salicylic acid (SA) on respiration and mitochondrial function was examined in tobacco (Nicotiana tabacum) suspension cell cultures in the range of 0.01 to 5 mm. Cells rapidly accumulated SA up to 10-fold of the externally applied concentrations. At the lower concentrations, SA accumulation was transitory...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.031039
更新日期:2004-01-01 00:00:00
abstract::The ictA gene, renamed ndhL in this paper, essential to inorganic carbon transport of Synechocystis PCC6803, was expressed in Eschericia coli as a fusion protein with glutathione S-transferase. An antibody was raised against this fusion protein. Western analysis of the thylakoid membrane of wild-type (WT) Synechocysti...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.99.4.1604
更新日期:1992-08-01 00:00:00
abstract::The effect of seed coat removal on the synthesis of alpha-amylase isoenzymes in wheat was investigated. The immature wheat endosperm-aleurone (seed coat and embryo detached) produced considerably less alpha-amylase activity than immature whole or de-embryonated wheat kernels, when incubated under identical conditions ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.67.1.89
更新日期:1981-01-01 00:00:00
abstract::Induction of ferric reductase activity in dicots and nongrass monocots is a well-recognized response to Fe deficiency. Recent evidence has shown that Cu deficiency also induces plasma membrane Fe reduction. In this study we investigated whether other nutrient deficiencies could also induce ferric reductase activity in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.3.1061
更新日期:1997-07-01 00:00:00
abstract::We have previously identified GAMYB, a gibberellin (GA)-regulated transcriptional activator of alpha-amylase gene expression, in aleurone cells of barley (Hordeum vulgare). To examine the regulation of GAMYB expression, we describe the use of nuclear run-on experiments to show that GA causes a 2-fold increase in the r...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.010918
更新日期:2002-05-01 00:00:00
abstract::The marine dinoflagellate Amphidinium carterae (Plymouth 450) releases several water-soluble peridinin-chlorophyll a proteins after freezethawing. These chromoproteins have a molecular weight of 39.2 x 10(3) and are comprised of noncovalently bound peridinin and chlorophyll a and a nonoligomeric protein. They have dis...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.57.2.297
更新日期:1976-02-01 00:00:00
abstract::We have cloned and characterized a cDNA encoding a maize (Zea mays L.) heat shock protein (HSP), HSP26. The mRNA of HSP26 is present as a single mRNA species of 1.1 kilobase pairs in size and is detectable when maize seedlings are treated at 40 degrees C but not at 28 degrees C. Accumulation of HSP26 mRNA was detected...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.4.1321
更新日期:1990-08-01 00:00:00
abstract::While membrane transporters mediating ammonium uptake across the plasma membrane have been well described at the molecular level, little is known about compartmentation and cellular export of ammonium. (The term ammonium is used to denote both NH3 and NH4+ and chemical symbols are used when specificity is required.) W...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.051268
更新日期:2005-02-01 00:00:00
abstract::The effect of ethylene on the uptake, distribution and polar transport of C(14) from indole-3-acetic acid-2-C(14) and naphthalene acetic acid-1-C(14) in tissue sections was studied. Test species were cotton (Gossypium hirsutum, L.) and cowpea (Vigna sinensis, Endl.). Generally, incubation of tissue or intact plants wi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.1.45
更新日期:1966-01-01 00:00:00
abstract::The presence of multiple forms of alpha-amylase in gibberellic acid-treated embryoless barley half-seeds was demonstrated by separation on diethylaminoethyl-Sephadex and isoelectric focusing polyacrylamide gel disc electrophoresis. Two major alpha-amylase fractions (A and B), each consisting of two to three isozyme co...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.46.4.586
更新日期:1970-10-01 00:00:00
abstract::Reactive oxygen species (ROS) are potent signal molecules rapidly generated in response to stress. Detection of pathogen-associated molecular patterns induces a transient apoplastic ROS through the function of the NADPH respiratory burst oxidase homologs D (RbohD). However, little is known about the regulation of path...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.154567
更新日期:2010-09-01 00:00:00
abstract::Trees typically experience large diurnal depressions in water potential, which may impede carbon export from leaves during the day because the xylem is the source of water for the phloem. As water potential becomes more negative, higher phloem osmotic concentrations are needed to draw water in from the xylem. Generati...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.20.00426
更新日期:2020-08-01 00:00:00
abstract::Previously, we reported that inorganic phosphate (Pi) deprivation of Brassica nigra suspension cells or seedlings leads to a progressive increase in the alpha: beta-subunit ratio of the inorganic pyrophosphate (PPi)-dependent phosphofructokinase (PFP) and that this coincides with a marked enhancement in the enzyme's a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.1.343
更新日期:1996-09-01 00:00:00
abstract::A radioimmunoassay for glyceollin I, the major phytoalexin produced by soybean (Glycine max [L.] Merr.), has been developed. Antibodies were raised in rabbits against a glyceollin I-bovine serum albumin conjugate. The antisera were used to establish a radioimmunoassay for glyceollin I using [(125)I]glyceollin I as the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.73.2.233
更新日期:1983-10-01 00:00:00
abstract::The surface tension (γ) of xylem sap plays a key role in stabilizing air-water interfaces at the pits between water- and gas-filled conduits to avoid air seeding at low water potentials. We studied seasonal changes in xylem sap γ in Picea abies and Pinus mugo growing at the alpine timberline. We analyzed their vulnera...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01053
更新日期:2017-11-01 00:00:00
abstract::In climates that experience short growing seasons due to drought, heat, or end-of-season frost, early flowering is a highly desirable trait for chickpea (Cicer arietinum). In this study, we mapped, sequenced, and characterized Early flowering3 (Efl3), an ortholog of Arabidopsis (Arabidopsis thaliana) EARLY FLOWERING3 ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00082
更新日期:2017-10-01 00:00:00
abstract::Respiratory control ratios between 2.0 and 9.0 were obtained by comparison of the respiratory rates of cabbage mitochondria in the presence and in the absence of individual components of the system used to provide ADP and by comparing the rates before and after exhaustion of added ADP. These results indicate that resp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.3.327
更新日期:1968-03-01 00:00:00
abstract::Storage compound accumulation during seed development prepares the next generation of plants for survival. Therefore, processes involved in the regulation and synthesis of storage compound accumulation during seed development bear relevance to germination and seedling establishment. The wrinkled1 (wri1) mutant of Arab...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.079574
更新日期:2006-06-01 00:00:00
abstract::Gas-liquid chromatography-mass spectrometry (GLC-MS) has been used for the separation, detection, and identification of 1,4-benzoxazin-3-ones (hydroxamic acids and lactams) and benzoxazolinones found in maize (Zea mays L.) extracts. Compounds of interest were partitioned into ethyl acetate from aqueous maize seedling ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.63.1.9
更新日期:1979-01-01 00:00:00
abstract::Dehydration inhibits petal expansion resulting in abnormal flower opening and results in quality loss during the marketing of cut flowers. We constructed a suppression subtractive hybridization library from rose (Rosa hybrida) flowers containing 3,513 unique expressed sequence tags and analyzed their expression profil...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.112.207720
更新日期:2012-12-01 00:00:00
abstract::Orcinol O-methyltransferase (OOMT) 1 and 2 catalyze the last two steps of the biosynthetic pathway leading to the phenolic methyl ether 3,5-dimethoxytoluene (DMT), the major scent compound of many rose (Rosa x hybrida) varieties. Modern roses are descended from both European and Chinese species, the latter being produ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.070961
更新日期:2006-01-01 00:00:00
abstract::We have used cDNA microarray analysis to examine changes in gene expression during embryo development in soybean (Glycine max) and to compare gene expression profiles of two soybean cultivars that differ in seed isoflavonoid content. The analysis identified 5,910 genes that were differentially expressed in both soybea...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.086306
更新日期:2007-01-01 00:00:00
abstract::The stimulation of the Hill reaction in CO(2)-depleted broken chloroplasts (Pisum sativum L. cv Rondo) by the total amount of dissolved CO(2) and HCO(3) (-) (bicarbonate(*)) was measured at several formate concentrations. Formate appears to be a competitive inhibitor of the bicarbonate(*) stimulation of electron flow....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.1.146
更新日期:1984-05-01 00:00:00
abstract::We identified a dwarf transgenic hybrid poplar (Populus tremula x Populus alba) after screening of 627 independent activation-tagged transgenic lines in tissue culture, greenhouse, and field environments. The cause of the phenotype was a hyperactivated gene encoding GA 2-oxidase (GA2ox), the major gibberellin (GA) cat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.020354
更新日期:2003-07-01 00:00:00
abstract::The evolution of C4 photosynthesis in many taxa involves the establishment of a two-celled photorespiratory CO2 pump, termed C2 photosynthesis. How C3 species evolved C2 metabolism is critical to understanding the initial phases of C4 plant evolution. To evaluate early events in C4 evolution, we compared leaf anatomy,...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.221119
更新日期:2013-11-01 00:00:00
abstract::C(4) photosynthesis has evolved multiple times from ancestral C(3) species. Carbonic anhydrase (CA) catalyzes the reversible hydration of CO(2) and is involved in both C(3) and C(4) photosynthesis; however, its roles and the intercellular and intracellular locations of the majority of its activity differ between C(3) ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.137513
更新日期:2009-07-01 00:00:00
abstract::Comparative genomics provides insight into the evolutionary dynamics that shape discrete sequences as well as whole genomes. To advance comparative genomics within the Brassicaceae, we have end sequenced 23,136 medium-sized insert clones from Boechera stricta, a wild relative of Arabidopsis (Arabidopsis thaliana). A s...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.105.073981
更新日期:2006-04-01 00:00:00
abstract::The enzyme aldose (aldehyde) reductase was partially purified (142-fold) and characterized from Euonymus japonica leaves. The reductase, a dimer, had an average molecular weight of 67,000 as determined by gel filtration on Sephadex G-100. The enzyme was NADPH specific and reduced a broad range of substrates including ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.4.972
更新日期:1986-04-01 00:00:00
abstract::Intact peroxisomes were prepared from green leaves of a number of C(3) species, both monocots and dicots. A protoplast extract from which chloroplasts have been removed by a 1-minute 10,000g centrifugation was applied to a step gradient of 5, 15, 30, and 45% Percoll containing 0.5 molar sucrose, 0.1% BSA, and 25 milli...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.4.1213
更新日期:1982-10-01 00:00:00
abstract::High specific radioactivity (26.3 mc/mmole) racemic 2-(14)C-abscisic acid was synthesized. An aliquot of abscisic acid, 1.2 x 10(-4)m in aqueous methanolic solution, was applied to the surface of either a cotyledon or the first true leaf of 8- to 32-day-old cotton seedlings (Gossypium hirsutum L.). After various inter...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.52.5.443
更新日期:1973-11-01 00:00:00