Down-regulation of Kelch domain-containing F-box protein in Arabidopsis enhances the production of (poly)phenols and tolerance to ultraviolet radiation.

Abstract:

:Phenylpropanoid biosynthesis in plants engenders myriad phenolics with diverse biological functions. Phenylalanine ammonia-lyase (PAL) is the first committed enzyme in the pathway, directing primary metabolic flux into a phenylpropanoid branch. Previously, we demonstrated that the Arabidopsis (Arabidopsis thaliana) Kelch domain-containing F-box proteins, AtKFB01, AtKFB20, and AtKFB50, function as the negative regulators controlling phenylpropanoid biosynthesis via mediating PAL's ubiquitination and subsequent degradation. Here, we reveal that Arabidopsis KFB39, a close homolog of AtKFB50, also interacts physically with PAL isozymes and modulates PAL stability and activity. Disturbing the expression of KFB39 reciprocally affects the accumulation/deposition of a set of phenylpropanoid end products, suggesting that KFB39 is an additional posttranslational regulator responsible for the turnover of PAL and negatively controlling phenylpropanoid biosynthesis. Furthermore, we discover that exposure of Arabidopsis to ultraviolet (UV)-B radiation suppresses the expression of all four KFB genes while inducing the transcription of PAL isogenes; these data suggest that Arabidopsis consolidates both transcriptional and posttranslational regulation mechanisms to maximize its responses to UV light stress. Simultaneous down-regulation of all four identified KFBs significantly enhances the production of (poly)phenols and the plant's tolerance to UV irradiation. This study offers a biotechnological approach for engineering the production of useful phenolic chemicals and for increasing a plant's resistance to environmental stress.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Zhang X,Gou M,Guo C,Yang H,Liu CJ

doi

10.1104/pp.114.249136

subject

Has Abstract

pub_date

2015-02-01 00:00:00

pages

337-50

issue

2

eissn

0032-0889

issn

1532-2548

pii

pp.114.249136

journal_volume

167

pub_type

杂志文章
  • Salicylic acid is an uncoupler and inhibitor of mitochondrial electron transport.

    abstract::The effect of salicylic acid (SA) on respiration and mitochondrial function was examined in tobacco (Nicotiana tabacum) suspension cell cultures in the range of 0.01 to 5 mm. Cells rapidly accumulated SA up to 10-fold of the externally applied concentrations. At the lower concentrations, SA accumulation was transitory...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.031039

    authors: Norman C,Howell KA,Millar AH,Whelan JM,Day DA

    更新日期:2004-01-01 00:00:00

  • Identification and Characterization of the ictA/ndhL Gene Product Essential to Inorganic Carbon Transport of Synechocystis PCC6803.

    abstract::The ictA gene, renamed ndhL in this paper, essential to inorganic carbon transport of Synechocystis PCC6803, was expressed in Eschericia coli as a fusion protein with glutathione S-transferase. An antibody was raised against this fusion protein. Western analysis of the thylakoid membrane of wild-type (WT) Synechocysti...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.4.1604

    authors: Ogawa T

    更新日期:1992-08-01 00:00:00

  • Alpha-amylase synthesis in wheat kernels as influenced by the seed coat.

    abstract::The effect of seed coat removal on the synthesis of alpha-amylase isoenzymes in wheat was investigated. The immature wheat endosperm-aleurone (seed coat and embryo detached) produced considerably less alpha-amylase activity than immature whole or de-embryonated wheat kernels, when incubated under identical conditions ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.1.89

    authors: Marchylo BA,Lacroix LJ,Kruger JE

    更新日期:1981-01-01 00:00:00

  • Induction of the Root Cell Plasma Membrane Ferric Reductase (An Exclusive Role for Fe and Cu).

    abstract::Induction of ferric reductase activity in dicots and nongrass monocots is a well-recognized response to Fe deficiency. Recent evidence has shown that Cu deficiency also induces plasma membrane Fe reduction. In this study we investigated whether other nutrient deficiencies could also induce ferric reductase activity in...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.3.1061

    authors: Cohen CK,Norvell WA,Kochian LV

    更新日期:1997-07-01 00:00:00

  • Gibberellin signaling in barley aleurone cells. Control of SLN1 and GAMYB expression.

    abstract::We have previously identified GAMYB, a gibberellin (GA)-regulated transcriptional activator of alpha-amylase gene expression, in aleurone cells of barley (Hordeum vulgare). To examine the regulation of GAMYB expression, we describe the use of nuclear run-on experiments to show that GA causes a 2-fold increase in the r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010918

    authors: Gubler F,Chandler PM,White RG,Llewellyn DJ,Jacobsen JV

    更新日期:2002-05-01 00:00:00

  • Peridinin-Chlorophyll a Proteins of the Dinoflagellate Amphidinium carterae (Plymouth 450).

    abstract::The marine dinoflagellate Amphidinium carterae (Plymouth 450) releases several water-soluble peridinin-chlorophyll a proteins after freezethawing. These chromoproteins have a molecular weight of 39.2 x 10(3) and are comprised of noncovalently bound peridinin and chlorophyll a and a nonoligomeric protein. They have dis...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.57.2.297

    authors: Haxo FT,Kycia JH,Somers GF,Bennett A,Siegelman HW

    更新日期:1976-02-01 00:00:00

  • Cloning, sequence analysis, and expression of a cDNA encoding a plastid-localized heat shock protein in maize.

    abstract::We have cloned and characterized a cDNA encoding a maize (Zea mays L.) heat shock protein (HSP), HSP26. The mRNA of HSP26 is present as a single mRNA species of 1.1 kilobase pairs in size and is detectable when maize seedlings are treated at 40 degrees C but not at 28 degrees C. Accumulation of HSP26 mRNA was detected...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.4.1321

    authors: Nieto-Sotelo J,Vierling E,Ho TH

    更新日期:1990-08-01 00:00:00

  • Tonoplast intrinsic proteins AtTIP2;1 and AtTIP2;3 facilitate NH3 transport into the vacuole.

    abstract::While membrane transporters mediating ammonium uptake across the plasma membrane have been well described at the molecular level, little is known about compartmentation and cellular export of ammonium. (The term ammonium is used to denote both NH3 and NH4+ and chemical symbols are used when specificity is required.) W...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.051268

    authors: Loqué D,Ludewig U,Yuan L,von Wirén N

    更新日期:2005-02-01 00:00:00

  • Effects of ethylene on auxin transport.

    abstract::The effect of ethylene on the uptake, distribution and polar transport of C(14) from indole-3-acetic acid-2-C(14) and naphthalene acetic acid-1-C(14) in tissue sections was studied. Test species were cotton (Gossypium hirsutum, L.) and cowpea (Vigna sinensis, Endl.). Generally, incubation of tissue or intact plants wi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.1.45

    authors: Morgan PW,Gausman HW

    更新日期:1966-01-01 00:00:00

  • alpha-Amylase Isozymes in Gibberellic Acid-treated Barley Half-seeds.

    abstract::The presence of multiple forms of alpha-amylase in gibberellic acid-treated embryoless barley half-seeds was demonstrated by separation on diethylaminoethyl-Sephadex and isoelectric focusing polyacrylamide gel disc electrophoresis. Two major alpha-amylase fractions (A and B), each consisting of two to three isozyme co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.4.586

    authors: Tanaka Y,Akazawa T

    更新日期:1970-10-01 00:00:00

  • Ethylene signaling regulates accumulation of the FLS2 receptor and is required for the oxidative burst contributing to plant immunity.

    abstract::Reactive oxygen species (ROS) are potent signal molecules rapidly generated in response to stress. Detection of pathogen-associated molecular patterns induces a transient apoplastic ROS through the function of the NADPH respiratory burst oxidase homologs D (RbohD). However, little is known about the regulation of path...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.154567

    authors: Mersmann S,Bourdais G,Rietz S,Robatzek S

    更新日期:2010-09-01 00:00:00

  • Leaf Carbon Export and Nonstructural Carbohydrates in Relation to Diurnal Water Dynamics in Mature Oak Trees.

    abstract::Trees typically experience large diurnal depressions in water potential, which may impede carbon export from leaves during the day because the xylem is the source of water for the phloem. As water potential becomes more negative, higher phloem osmotic concentrations are needed to draw water in from the xylem. Generati...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00426

    authors: Gersony JT,Hochberg U,Rockwell FE,Park M,Gauthier PPG,Holbrook NM

    更新日期:2020-08-01 00:00:00

  • Purification and characterization of pyrophosphate-dependent phosphofructokinase from phosphate-starved Brassica nigra suspension cells.

    abstract::Previously, we reported that inorganic phosphate (Pi) deprivation of Brassica nigra suspension cells or seedlings leads to a progressive increase in the alpha: beta-subunit ratio of the inorganic pyrophosphate (PPi)-dependent phosphofructokinase (PFP) and that this coincides with a marked enhancement in the enzyme's a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.1.343

    authors: Theodorou ME,Plaxton WC

    更新日期:1996-09-01 00:00:00

  • Development of a radioimmunoassay for the soybean phytoalexin glyceollin I.

    abstract::A radioimmunoassay for glyceollin I, the major phytoalexin produced by soybean (Glycine max [L.] Merr.), has been developed. Antibodies were raised in rabbits against a glyceollin I-bovine serum albumin conjugate. The antisera were used to establish a radioimmunoassay for glyceollin I using [(125)I]glyceollin I as the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.2.233

    authors: Moesta P,Hahn MG,Grisebach H

    更新日期:1983-10-01 00:00:00

  • Xylem Sap Surface Tension May Be Crucial for Hydraulic Safety.

    abstract::The surface tension (γ) of xylem sap plays a key role in stabilizing air-water interfaces at the pits between water- and gas-filled conduits to avoid air seeding at low water potentials. We studied seasonal changes in xylem sap γ in Picea abies and Pinus mugo growing at the alpine timberline. We analyzed their vulnera...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01053

    authors: Losso A,Beikircher B,Dämon B,Kikuta S,Schmid P,Mayr S

    更新日期:2017-11-01 00:00:00

  • The Chickpea Early Flowering 1 (Efl1) Locus Is an Ortholog of Arabidopsis ELF3.

    abstract::In climates that experience short growing seasons due to drought, heat, or end-of-season frost, early flowering is a highly desirable trait for chickpea (Cicer arietinum). In this study, we mapped, sequenced, and characterized Early flowering3 (Efl3), an ortholog of Arabidopsis (Arabidopsis thaliana) EARLY FLOWERING3 ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00082

    authors: Ridge S,Deokar A,Lee R,Daba K,Macknight RC,Weller JL,Tar'an B

    更新日期:2017-10-01 00:00:00

  • Intermediate reactions of oxidative phosphorylation in mitochondria from cabbage.

    abstract::Respiratory control ratios between 2.0 and 9.0 were obtained by comparison of the respiratory rates of cabbage mitochondria in the presence and in the absence of individual components of the system used to provide ADP and by comparing the rates before and after exhaustion of added ADP. These results indicate that resp...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.3.327

    authors: Lotlikar PD,Remmert LF

    更新日期:1968-03-01 00:00:00

  • WRI1 is required for seed germination and seedling establishment.

    abstract::Storage compound accumulation during seed development prepares the next generation of plants for survival. Therefore, processes involved in the regulation and synthesis of storage compound accumulation during seed development bear relevance to germination and seedling establishment. The wrinkled1 (wri1) mutant of Arab...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.079574

    authors: Cernac A,Andre C,Hoffmann-Benning S,Benning C

    更新日期:2006-06-01 00:00:00

  • Identification of 1,4-Benzoxazin-3-ones in Maize Extracts by Gas-Liquid Chromatography and Mass Spectrometry.

    abstract::Gas-liquid chromatography-mass spectrometry (GLC-MS) has been used for the separation, detection, and identification of 1,4-benzoxazin-3-ones (hydroxamic acids and lactams) and benzoxazolinones found in maize (Zea mays L.) extracts. Compounds of interest were partitioned into ethyl acetate from aqueous maize seedling ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.1.9

    authors: Woodward MD,Corcuera LJ,Schnoes HK,Helgeson JP,Upper CD

    更新日期:1979-01-01 00:00:00

  • RhNAC2 and RhEXPA4 are involved in the regulation of dehydration tolerance during the expansion of rose petals.

    abstract::Dehydration inhibits petal expansion resulting in abnormal flower opening and results in quality loss during the marketing of cut flowers. We constructed a suppression subtractive hybridization library from rose (Rosa hybrida) flowers containing 3,513 unique expressed sequence tags and analyzed their expression profil...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.207720

    authors: Dai F,Zhang C,Jiang X,Kang M,Yin X,Lü P,Zhang X,Zheng Y,Gao J

    更新日期:2012-12-01 00:00:00

  • Role of petal-specific orcinol O-methyltransferases in the evolution of rose scent.

    abstract::Orcinol O-methyltransferase (OOMT) 1 and 2 catalyze the last two steps of the biosynthetic pathway leading to the phenolic methyl ether 3,5-dimethoxytoluene (DMT), the major scent compound of many rose (Rosa x hybrida) varieties. Modern roses are descended from both European and Chinese species, the latter being produ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.070961

    authors: Scalliet G,Lionnet C,Le Bechec M,Dutron L,Magnard JL,Baudino S,Bergougnoux V,Jullien F,Chambrier P,Vergne P,Dumas C,Cock JM,Hugueney P

    更新日期:2006-01-01 00:00:00

  • Transcriptome analysis reveals a critical role of CHS7 and CHS8 genes for isoflavonoid synthesis in soybean seeds.

    abstract::We have used cDNA microarray analysis to examine changes in gene expression during embryo development in soybean (Glycine max) and to compare gene expression profiles of two soybean cultivars that differ in seed isoflavonoid content. The analysis identified 5,910 genes that were differentially expressed in both soybea...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.086306

    authors: Dhaubhadel S,Gijzen M,Moy P,Farhangkhoee M

    更新日期:2007-01-01 00:00:00

  • Reevaluation of the role of bicarbonate and formate in the regulation of photosynthetic electron flow in broken chloroplasts.

    abstract::The stimulation of the Hill reaction in CO(2)-depleted broken chloroplasts (Pisum sativum L. cv Rondo) by the total amount of dissolved CO(2) and HCO(3) (-) (bicarbonate(*)) was measured at several formate concentrations. Formate appears to be a competitive inhibitor of the bicarbonate(*) stimulation of electron flow....

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.1.146

    authors: Snel JF,van Rensen JJ

    更新日期:1984-05-01 00:00:00

  • Activation tagging of a dominant gibberellin catabolism gene (GA 2-oxidase) from poplar that regulates tree stature.

    abstract::We identified a dwarf transgenic hybrid poplar (Populus tremula x Populus alba) after screening of 627 independent activation-tagged transgenic lines in tissue culture, greenhouse, and field environments. The cause of the phenotype was a hyperactivated gene encoding GA 2-oxidase (GA2ox), the major gibberellin (GA) cat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.020354

    authors: Busov VB,Meilan R,Pearce DW,Ma C,Rood SB,Strauss SH

    更新日期:2003-07-01 00:00:00

  • Initial events during the evolution of C4 photosynthesis in C3 species of Flaveria.

    abstract::The evolution of C4 photosynthesis in many taxa involves the establishment of a two-celled photorespiratory CO2 pump, termed C2 photosynthesis. How C3 species evolved C2 metabolism is critical to understanding the initial phases of C4 plant evolution. To evaluate early events in C4 evolution, we compared leaf anatomy,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.221119

    authors: Sage TL,Busch FA,Johnson DC,Friesen PC,Stinson CR,Stata M,Sultmanis S,Rahman BA,Rawsthorne S,Sage RF

    更新日期:2013-11-01 00:00:00

  • Loss of the transit peptide and an increase in gene expression of an ancestral chloroplastic carbonic anhydrase were instrumental in the evolution of the cytosolic C4 carbonic anhydrase in Flaveria.

    abstract::C(4) photosynthesis has evolved multiple times from ancestral C(3) species. Carbonic anhydrase (CA) catalyzes the reversible hydration of CO(2) and is involved in both C(3) and C(4) photosynthesis; however, its roles and the intercellular and intracellular locations of the majority of its activity differ between C(3) ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.137513

    authors: Tanz SK,Tetu SG,Vella NG,Ludwig M

    更新日期:2009-07-01 00:00:00

  • Partial shotgun sequencing of the Boechera stricta genome reveals extensive microsynteny and promoter conservation with Arabidopsis.

    abstract::Comparative genomics provides insight into the evolutionary dynamics that shape discrete sequences as well as whole genomes. To advance comparative genomics within the Brassicaceae, we have end sequenced 23,136 medium-sized insert clones from Boechera stricta, a wild relative of Arabidopsis (Arabidopsis thaliana). A s...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.073981

    authors: Windsor AJ,Schranz ME,Formanová N,Gebauer-Jung S,Bishop JG,Schnabelrauch D,Kroymann J,Mitchell-Olds T

    更新日期:2006-04-01 00:00:00

  • Purification and Properties of an NADPH-Aldose Reductase (Aldehyde Reductase) from Euonymus japonica Leaves.

    abstract::The enzyme aldose (aldehyde) reductase was partially purified (142-fold) and characterized from Euonymus japonica leaves. The reductase, a dimer, had an average molecular weight of 67,000 as determined by gel filtration on Sephadex G-100. The enzyme was NADPH specific and reduced a broad range of substrates including ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.4.972

    authors: Negm FB

    更新日期:1986-04-01 00:00:00

  • Isolation and purification of intact peroxisomes from green leaf tissue.

    abstract::Intact peroxisomes were prepared from green leaves of a number of C(3) species, both monocots and dicots. A protoplast extract from which chloroplasts have been removed by a 1-minute 10,000g centrifugation was applied to a step gradient of 5, 15, 30, and 45% Percoll containing 0.5 molar sucrose, 0.1% BSA, and 25 milli...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.4.1213

    authors: Schmitt MR,Edwards GE

    更新日期:1982-10-01 00:00:00

  • Absorption and distribution of high specific radioactivity 2-C-abscisic Acid in cotton seedlings.

    abstract::High specific radioactivity (26.3 mc/mmole) racemic 2-(14)C-abscisic acid was synthesized. An aliquot of abscisic acid, 1.2 x 10(-4)m in aqueous methanolic solution, was applied to the surface of either a cotyledon or the first true leaf of 8- to 32-day-old cotton seedlings (Gossypium hirsutum L.). After various inter...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.5.443

    authors: Shindy WW,Asmundson CM,Smith OE,Kumamoto J

    更新日期:1973-11-01 00:00:00