Abstract:
:The stimulation of the Hill reaction in CO(2)-depleted broken chloroplasts (Pisum sativum L. cv Rondo) by the total amount of dissolved CO(2) and HCO(3) (-) (bicarbonate(*)) was measured at several formate concentrations. Formate appears to be a competitive inhibitor of the bicarbonate(*) stimulation of electron flow. From these experiments we have obtained a reactivation constant (K(r)) of 78 +/- 31 micromolar NaHCO(3) and an inhibition constant (K(i)) of 2.0 +/- 0.7 millimolar HCOONa at pH 6.5. In the absence of formate, significant electron flow was measured at a bicarbonate(*) concentration well below K(r), suggesting that electron flow from Q, the primary electron acceptor of photosystem II, to plastoquinone can proceed when no bicarbonate(*) is bound to the regulatory site at the Q(B)-protein. If so, bicarbonate(*) stimulation of electron flow is mainly a diminution of the inhibition of electron flow by formate. In view of the results, it is proposed that regulation of linear electron flow by bicarbonate(*) and formate is a mechanism that could link cell metabolism to photosynthetic electron flow.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Snel JF,van Rensen JJdoi
10.1104/pp.75.1.146subject
Has Abstractpub_date
1984-05-01 00:00:00pages
146-50issue
1eissn
0032-0889issn
1532-2548journal_volume
75pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::We have previously shown that in the red alga Rhodella violacea, exposure to continuous low intensities of light 2 (green light) or near-saturating intensities of white light induces a DeltapH-dependent PSII fluorescence quenching. In this article we further characterize this fluorescence quenching by using white, sat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.118.1.103
更新日期:1998-09-01 00:00:00
abstract::Glycoprotein nonspecific elicitor (NSE) and a specific elicitor preparation from intercellular fluids (SE) of tomato (Lycopersicon esculentum Mill. cv Bonny Best or Potentate) infected with race 2.4.5 of Cladosporium fulvum Cooke [syn. Fulvia fulva (Cooke) Ciferri] were injected into cv Sonatine (resistant to race 2.4...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.90.3.867
更新日期:1989-07-01 00:00:00
abstract::Reactive oxygen species act as signaling molecules but can also directly provoke cellular damage by rapidly oxidizing cellular components, including lipids. We developed a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry-based quantitative method that allowed us to discriminate b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.125690
更新日期:2008-10-01 00:00:00
abstract::The effects of representative respiratory inhibitors were investigated on the coupled respiration of mung bean mitochondria using succinate and l-malate as substrates. The inhibitors studied were: (I) malonate, (II) amytal and rotenone, (III) antimycin A and 2-n-nonyl-4-hydroxyquinoline N-oxide (NOQNO), and (IV) cyani...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.11.1535
更新日期:1967-11-01 00:00:00
abstract::When ribulose-1,5-bisphosphate carboxylase is assayed under N(2) using [(3)H]ribulose 1,5-bisphosphate and (14)CO(2), [(3)H]3-phosphoglycerate and [(14)C]3-phosphoglycerate are produced in nonstoichiometric amounts in a ratio which approaches 7 at low concentrations of CO(2) (2 micromolar) assuming a 1:1 ratio at V(ma...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.80.4.1055
更新日期:1986-04-01 00:00:00
abstract::4-Hydroxyphenylpyruvate dioxygenase (4HPPD) catalyzes the formation of homogentisate (2,5-dihydroxyphenylacetate) from p-hydroxyphenylpyruvate and molecular oxygen. In plants this enzyme activity is involved in two distinct metabolic processes, the biosynthesis of prenylquinones and the catabolism of tyrosine. We repo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.119.4.1507
更新日期:1999-04-01 00:00:00
abstract::DEAD-box RNA helicases (DBRHs) modulate RNA secondary structure, allowing RNA molecules to adopt the conformations required for interaction with their target proteins. RH50 is a chloroplast-located DBRH that colocalizes and is coexpressed with GUN1, a central factor in chloroplast-to-nucleus signaling. When combined w...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01545
更新日期:2018-01-01 00:00:00
abstract::The nature of the association between nitrate reductase (NR) and membranes was examined. Nitrate reductase activity (NRA) associated with the microsomal fraction of barley (Hordeum vulgare L.) roots amounted to 0.6 to 0.8% of soluble NRA following sonication in the presence of 250 mM KI and repeated osmotic shock. T...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.3.925
更新日期:1994-01-01 00:00:00
abstract::A mutant of Arabidopsis thaliana has been isolated in which ribulose-1,5-bisphosphate carboxylase is present in a nonactivatable form in vivo. The mutation appears to affect carboxylase activation specifically, and not any other enzyme of the photosynthesis or photorespiratory cycles. The effect of the mutation on car...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.2.381
更新日期:1982-08-01 00:00:00
abstract::Pepino mosaic virus (PepMV) is a highly infectious potexvirus and a major disease of greenhouse tomato (Solanum lycopersicum) crops worldwide. Damage and economic losses caused by PepMV vary greatly and can be attributed to differential symptomatology caused by different PepMV isolates. Here, we used a custom-designed...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.173906
更新日期:2011-05-01 00:00:00
abstract::Formation of the riboside-5'-monophosphate is a general feature of the metabolism of cytokinins in plants. As part of a study of the biological significance of the nucleotide form of cytokinins, we analyzed a mutant of Arabidopsis thaliana deficient in adenine phosphoribosyltransferase (APRT) activity for its ability ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.95.3.900
更新日期:1991-03-01 00:00:00
abstract::Distribution of K, Ca, Cl, S, and P in freeze-dried sections of Arabidopsis flower stalk was analyzed by energy dispersive x-ray imaging. Concentrations of these elements in different cell types were quantified by microanalysis of single-cell samples and phloem exudates. Results showed a differential pattern of distri...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.124.2.599
更新日期:2000-10-01 00:00:00
abstract::The anatomy of strawberry (Fragaria x ananassa) fruit, in which the achene is found on the outer part of the fruit, makes it an excellent species for studying the regulation of fruit development. It can provide a model for the cross talk between primary and secondary metabolism, whose role is of pivotal importance in ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.120691
更新日期:2008-10-01 00:00:00
abstract::The combined action of the inhibitors antimycin A and cyanide with benzohydroxamic acid indicates the presence of a cyanide-resistant pathway of respiration in chick pea (Cicer arietinum L.) seeds. The appearance of this pathway takes place during germination. During the first 12 hours of germination, the respiration ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.4.524
更新日期:1977-10-01 00:00:00
abstract::Regulation of photosynthetic gene expression by plastid-encoded RNA polymerase (PEP) is essential for chloroplast development. The activity of PEP largely relies on at least 12 PEP-associated proteins (PAPs) encoded in the nuclear genome of plant cells. A recent model proposed that these PAPs regulate the establishmen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00248
更新日期:2017-05-01 00:00:00
abstract::Amino acids and amino acid analogs have been used in numerous genetic screens to isolate mutants deficient in amino acid biosynthetic pathways or in the regulation of amino acid metabolism. Several of these mutants exhibit relaxed feedback control of branched amino acid biosynthetic pathways and are thus resistant to ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.047506
更新日期:2004-10-01 00:00:00
abstract::Aspartate aminotransferase (AAT), a key enzyme in the biosynthesis of aspartate and asparagine, occurs as two forms in alfalfa (Medicago sativa L.), AAT-1 and AAT-2. Both forms were purified to near homogeneity, and high titer polyclonal antibodies produced to the native proteins. Alfalfa AAT-1 was purified from root ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.93.2.603
更新日期:1990-06-01 00:00:00
abstract::The capacity of sodium orthovanadate to inhibit the plasmalemma H(+) ATPase of Nitella translucens internodal cells in vivo was tested. Here we show that 1 millimolar vanadate added externally depolarizes strongly and permanently the membrane potential, both in dark and light, to the Nernst potential for potassium con...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.1.91
更新日期:1991-05-01 00:00:00
abstract::We have characterized the gravitropic response of inflorescence stems in Arabidopsis thaliana. When the inflorescence stems were placed horizontally, they curved upward about 90 degrees within 90 min in darkness at 23 degrees C, exhibiting strong negative gravitropism. Decapitated stem segments (without all flowers, f...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.3.933
更新日期:1996-03-01 00:00:00
abstract::Biosynthesis of the polyamines putrescine, spermidine, and spermine is induced in response to pathogen infection of plants. Putrescine, which is produced from Arg, serves as a metabolic precursor for longer polyamines, including spermidine and spermine. Polyamine acetylation, which has important regulatory functions i...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00446
更新日期:2016-06-01 00:00:00
abstract::Metal accumulation in seeds is a prerequisite for germination and establishment of plants but also for micronutrient delivery to humans. To investigate metal transport processes and their interactions in seeds, we focused on METAL TOLERANCE PROTEIN8 (MTP8), a tonoplast transporter of the manganese (Mn) subclade of cat...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01646
更新日期:2017-07-01 00:00:00
abstract::The role of H(+) excretion in auxin-induced growth of soybean hypocotyl tissues has been investigated, using tissues whose cuticle was rendered permeable to protons or buffers by scarification (scrubbing). Indoleacetic acid induces both elongation and H(+) excretion after a lag of 10 to 12 minutes. Cycloheximide inhib...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.3.433
更新日期:1980-09-01 00:00:00
abstract::Reducing the level of endogenous ethylene in detached Shamouti oranges (Citrus sinensis L. Osbeck) by means of subatmospheric pressure did not alter the rate of chlorophyll destruction and color changes during the first 8 days after harvest in the presence or absence of exogenous ethylene. Reducing the activity of eth...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.57.5.836
更新日期:1976-05-01 00:00:00
abstract::A method has been devised for loading chloroplast inner envelope vesicles prepared from pea (Pisum sativum L. var Progress No. 9) or spinach (Spinacia oleracea L.) with 8-hydroxypyrene-1,3,6-trisulfonate (pyranine), a membrane impermeant, fluorescent pH indicator. Two known proton-linked transport activities of the in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.86.4.999
更新日期:1988-04-01 00:00:00
abstract::An experimental chemical N-[2-(2-oxo-1-imidazolidinyl)ethyl]-N'-phenylurea (EDU), is an effective protectant against acute and chronic foliar injury due to ozone (0(3)) when sprayed on intact leaves or supplied to the plants through soil application. An 0(3)-sensitive snap bean cultivar (Phaseolus vulgaris L. ;Bush Bl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.6.1444
更新日期:1982-06-01 00:00:00
abstract::When wheat (Triticum aestivum) seedlings are exposed to a cold temperature (2-4 degrees C) above 0 degrees C, sucrose accumulates and sucrose synthase activity increases. The effect of a cold period on the level of sucrose synthase (SS) was investigated. Using antibodies against wheat germ SS, Western blots studies sh...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.3.887
更新日期:1991-07-01 00:00:00
abstract::In order to study the effects of desiccation on a photosynthetic system, light harvesting and light-induced electron transport processes were examined in pea cotyledons at various moisture levels, using in vivo fluorescence excitation spectra and fluorescence induction kinetics. Water sorption isotherms yielded thermo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.79.1.248
更新日期:1985-09-01 00:00:00
abstract::Sucrose phosphate synthase and acid invertase activities in the mature leaves of roses (Rosa hybrida cv Golden Times) were greater in plants grown under a higher night temperature than under a lower temperature regime. In young shoots, the activity of acid invertase was promoted by the lower temperature while that of ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.84.2.447
更新日期:1987-06-01 00:00:00
abstract::Electron transport activity at the cell surface of intact oat seedlings (Avena sativa L. cv Garry) was examined by measuring the oxidation and/or reduction of agents in the medium bathing the roots. Oxidation of NADH with or without added electron acceptors and reduction of ferricyanide by an endogenous electron donor...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.76.2.386
更新日期:1984-10-01 00:00:00
abstract::Boron (B) polyol complexes have been isolated and characterized from the phloem sap of celery (Apium graveolens L.) and the extrafloral nectar of peach (Prunus persica L.). In celery the direct analysis of untreated phloem sap by matrix-assisted laser desorption-Fourier transform mass spectrometry, with verification b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.2.649
更新日期:1997-02-01 00:00:00