Abstract:
:Amino acids and amino acid analogs have been used in numerous genetic screens to isolate mutants deficient in amino acid biosynthetic pathways or in the regulation of amino acid metabolism. Several of these mutants exhibit relaxed feedback control of branched amino acid biosynthetic pathways and are thus resistant to accumulation of pathway end products. For example, feedback-regulated enzymes of the shikimate pathway are anthranilate synthase on the branch leading to Trp and chorismate mutase on the branch leading to Phe and Tyr. A feedback-insensitive mutant of anthranilate synthase alpha, trp5-1, is resistant to toxic Trp analogs. Mutants resistant to Phe have not previously been reported, and this article describes the isolation of the recessive Arabidopsis Phe insensitive growth mutant pig1-1 by a forward genetic screen. pig1-1 was not only tolerant to Phe, Tyr, and Trp, but also to other, not biosynthetically related amino acids. Amino acid contents in pig1-1 were significantly elevated with respect to wild-type controls but, in contrast to the wild type, dramatically decreased when plants were supplemented with 2 mm Phe. Protein contents were similar in the mutant and the wild type at all tested conditions. Phe catabolism was similar to the wild type in pig1-1 roots but was significantly increased in pig1-1 shoots. Phenylalanine uptake into the root, its root-to-shoot translocation, and Phe and phenylpropanoid contents were unaltered in pig1-1, indicating that pig1-1 is not affected in amino acid translocation or the shikimate pathway. Instead, the response of pig1-1 toward amino acid feeding indicates that amino acid metabolism is generally deregulated in pig1-1.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Voll LM,Allaire EE,Fiene G,Weber APdoi
10.1104/pp.104.047506subject
Has Abstractpub_date
2004-10-01 00:00:00pages
3058-69issue
2eissn
0032-0889issn
1532-2548pii
pp.104.047506journal_volume
136pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Biochemical changes related to cyanogenesis (hydrogen cyanide production) were monitored during maturation of black cherry (Prunus serotina Ehrh.) fruits. At weekly intervals from flowering until maturity, fruits (or selected parts thereof) were analyzed for (a) fresh and dry weights, (b) prunasin and amygdalin levels...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.98.4.1423
更新日期:1992-04-01 00:00:00
abstract::It has been suggested that jasmonic acid (JA) could be an integral part of a general signal transduction system regulating inducible defense genes in plants. It was reported that treatment with an elicitor (N-acetylchitoheptaose) induced production of phytoalexin in suspension-cultured rice (Oryza sativa L.) cells. In...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.2.387
更新日期:1996-02-01 00:00:00
abstract::The mechanism of sucrose transport was investigated in plasma membrane (PM) vesicles isolated from spinach (Spinacia oleracea L.) leaves. PM vesicles were isolated by aqueous two-phase partitioning and were equilibrated in pH 7.8 buffer containing K(+). The vesicles rapidly accumulated sucrose in the presence of a tra...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.2.615
更新日期:1991-06-01 00:00:00
abstract::Tomato Atypical Receptor Kinase 1 (TARK1) is a pseudokinase required for postinvasion immunity. TARK1 was originally identified as a target of the Xanthomonas euvesicatoria effector protein Xanthomonas outer protein N (XopN), a suppressor of early defense signaling. How TARK1 participates in immune signal transduction...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.01400
更新日期:2020-07-01 00:00:00
abstract::The LOSS OF APOMEIOSIS (LOA) locus is one of two dominant loci known to control apomixis in the eudicot Hieracium praealtum. LOA stimulates the differentiation of somatic aposporous initial cells after the initiation of meiosis in ovules. Aposporous initial cells undergo nuclear proliferation close to sexual megaspore...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.181164
更新日期:2011-11-01 00:00:00
abstract::The induction of phototropism in etiolated (dark-grown) seedlings exposed to an unidirectional pulse or extended irradiation with low fluence rate blue light (BL) requires the action of the phototropin (nph1) BL receptor. Although cryptochromes and phytochromes are not required for phototropic induction, these photore...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.2.826
更新日期:2001-06-01 00:00:00
abstract::Through a single genetic transformation in onion (Allium cepa), a crop recalcitrant to genetic transformation, we suppressed the lachrymatory factor synthase gene using RNA interference silencing in six plants. This reduced lachrymatory synthase activity by up to 1,544-fold, so that when wounded the onions produced si...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.123273
更新日期:2008-08-01 00:00:00
abstract::A new bacterial agglutinin was isolated from seeds of the soybean cultivar Clark. Purification was carried out by ammonium sulfate precipitation and ion-exchange chromatography. The agglutinin is a heat-labile glycoprotein most active at pH 4.0. Addition of Ca(2+), Mn(2+) and Mg(2+) did not enhance the agglutinating a...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.5.847
更新日期:1980-11-01 00:00:00
abstract::Waxy wheat (Triticum aestivum L.) lacks the waxy protein, which is also known as granule-bound starch synthase I (GBSSI). The starch granules of waxy wheat endosperm and pollen do not contain amylose and therefore stain red-brown with iodine. However, we observed that starch from pericarp tissue of waxy wheat stained ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.118.2.451
更新日期:1998-10-01 00:00:00
abstract::Interrupting each long night with a prolonged period of far red radiant energy resulted in the inhibition of floral initiation in cocklebur. Irradiations inducing different relative levels of P(FR) from 1 to 2% to 80% had about the same effect under 4-hour photoperiods. The lower levels of P(FR) induced by continuous ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.42.1.95
更新日期:1967-01-01 00:00:00
abstract::Chenopods synthesize betaine by a two-step oxidation of choline: choline --> betaine aldehyde --> betaine. The pathway is chloroplastic; the first step has been shown in isolated spinach (Spinacia oleracea L.) chloroplasts to be O(2)- and light-dependent, the role of light being to provide reducing power (P Weigel, EA...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.88.3.695
更新日期:1988-11-01 00:00:00
abstract::The influence of ozone on Ca(2+) transport in plant membranes from pinto bean (Phaseolus vulgaris L. var Pinto) leaves was investigated in vitro by means of a filtration method using purified vesicles. Two transport mechanisms located at the plasma membrane are involved in a response to ozone: (a) passive Ca(2+) influ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.94.2.788
更新日期:1990-10-01 00:00:00
abstract::THE PRESENT STUDY COMPARES THE BINDING AND INHIBITORY ACTIVITY OF TWO PHOTOSYSTEM II INHIBITORS: 3-(3,4-dichlorophenyl)-1,1-dimethylurea (diuron [DCMU]) and 2-chloro-4-(ethylamine)-6-(isopropyl amine)-S-triazene (atrazine). Chloroplasts isolated from naturally occurring triazine-susceptible and triazine-resistant biot...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.64.6.995
更新日期:1979-12-01 00:00:00
abstract::Sucrose synthase (Sus; EC 2.4.1.13) is a key enzyme of sucrose metabolism in plant cells, providing carbon for respiration and for the synthesis of cell wall polymers and starch. Since Sus is important for plant cell growth, insights into its structure, localization, and features are useful for defining the relationsh...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.115956
更新日期:2008-08-01 00:00:00
abstract::Severe Arabidopsis (Arabidopsis thaliana) gibberellin (GA)-deficient mutant ga1-3 fails to germinate and is impaired in floral organ development. In contrast, the ga1-3 gai-t6 rga-t2 rgl1-1 rgl2-1 mutant confers GA-independent seed germination and floral development. This fact suggests that GA-regulated transcriptomes...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.082289
更新日期:2006-10-01 00:00:00
abstract::We studied aquaporins in maize (Zea mays), an important crop in which numerous studies on plant water relations have been carried out. A maize cDNA, ZmTIP1, was isolated by reverse transcription-coupled PCR using conserved motifs from plant aquaporins. The derived amino acid sequence of ZmTIP1 shows 76% sequence ident...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.117.4.1143
更新日期:1998-08-01 00:00:00
abstract::Using novel specially designed instrumentation, fluorescence emission spectra were recorded from Arabidopsis (Arabidopsis thaliana) leaves during the induction period of dark to high-light adaptation in order to follow the spectral changes associated with the formation of nonphotochemical quenching. In addition to an ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.148213
更新日期:2010-03-01 00:00:00
abstract::The occurrence of proteins able to oxidize polyphenols even in the absence of H2O2 was recently reported in mung bean (Vigna radiata L.) hypocotyl cell wall extracts (R. Goldberg, A. Chabanet, A.M. Catesson [1993] In K.G. Welinder, S.K. Rasmussen, C. Penel, H. Greppin, eds, Plant Peroxidases: Biochemistry and Physiolo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.3.1095
更新日期:1994-11-01 00:00:00
abstract::Radioactive gibberellin A(5) ((3)H-GA(5)) was synthesized from gibberellic acid. When it was applied to dwarf peas grown in the dark, an average of 3% was converted to another acid gibberellin within 48 hours. The biological activity of the metabolite did not account for the response to applied GA(5). GA(5) is therefo...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.45.1.56
更新日期:1970-01-01 00:00:00
abstract::Plant viruses elicit the expression of common sets of genes in susceptible hosts. Studies in Arabidopsis (Arabidopsis thaliana) and tomato (Lycopersicon esculentum) indicate that at least one-third of the genes induced in common by viruses have been previously associated with plant defense and stress responses. The ge...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.104.056028
更新日期:2005-03-01 00:00:00
abstract::Branched-chain 2-oxo acids which are formed by transamination of leucine, isoleucine, and valine are metabolized by the peroxisomes from mung bean (Vigna radiata L.) hypocotyls. Acylcoenzyme A (CoA) thio ester intermediates of the pathways were separated by reversed-phase high performance liquid chromatography. Retent...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.4.1387
更新日期:1989-12-01 00:00:00
abstract::Genotypic variability in vulnerability of leaf xylem to water-stress-induced cavitation was determined in four sugarcane (Saccharum sp.) clones using detached leaf segments in a hydraulic conductivity apparatus. Vulnerability curves were constructed by plotting the percentage of maximum conductivity versus leaf water ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.2.1020
更新日期:1992-10-01 00:00:00
abstract::To study the effect of O(2) on the photosynthetic and glycolate pathways, maize leaves were exposed to (14)CO(2) during steady-state photosynthesis in 21 or 1% O(2). At the two O(2) concentrations after a (14)CO(2) pulse (4 seconds) followed by a (12)CO(2) chase, there was a slight difference in CO(2) uptake and in th...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.66.6.1079
更新日期:1980-12-01 00:00:00
abstract::We used a small oxygen electrode and a grating monochromator of 10 mmu half-band width to determine light-saturation curves of photosynthesis for films of Chlorella pyrenoidosa no more than 1 cell thick. All curves were referenced to the lightsaturated rate observed in 5 mw/cm(2) of 680 mmu. To a close approximation (...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.41.1.90
更新日期:1966-01-01 00:00:00
abstract::Root nodules were harvested from chamber-grown soybean (Glycine max L. Merrill cv Woodworth) plants throughout development. Apparent nitrogenase activity (acetylene reduction) peaked before seeds began to develop, but a significant amount of activity remained as the seeds matured. Nodule senescence was defined as the ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.71.4.797
更新日期:1983-04-01 00:00:00
abstract::Isoprene is formed in and emitted by plants and the reason for this apparent carbon waste is still unclear. It has been proposed that isoprene stabilizes cell and particularly chloroplast thylakoid membranes. We tested if membrane stabilization or isoprene reactivity with ozone induces protection against acute ozone e...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.126.3.993
更新日期:2001-07-01 00:00:00
abstract::The response of the actin cytoskeleton to nodulation (Nod) factors secreted by Rhizobium etli has been studied in living root hairs of bean (Phaseolus vulgaris) that were microinjected with fluorescein isothiocyanate-phalloidin. In untreated control cells or cells treated with the inactive chitin oligomer, the actin c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.116.3.871
更新日期:1998-03-01 00:00:00
abstract::A fructokinase (EC 2.7.1.4) was obtained from pea (Pisum sativum L.) seeds. This enzyme, termed fructokinase (fraction IV), was specific for fructose as substrate and had little activity with glucose or mannose. Excess fructose inhibited the enzyme at the optimum pH (8.2) but not at pH 6.6. MgATP was inhibitory at pH ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.60.5.666
更新日期:1977-11-01 00:00:00
abstract::Improving the performance of rice (Oryza sativa) under drought stress has the potential to significantly affect rice productivity. Here, we report that the ERF family transcription factor OsLG3 positively regulates drought tolerance in rice. In our previous work, we found that OsLG3 has a positive effect on rice grain...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01492
更新日期:2018-09-01 00:00:00
abstract::Ethylene supplied with indoleacetic acid at 0.1 and 1 mum inhibited elongation and enhanced swelling in epicotyls of decapitated and derooted pea seedlings (Pisum sativum L., var. Alaska). These growth responses were correlated with the development of cell walls rich in weak acid-extractable materials and pectic uroni...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.55.3.450
更新日期:1975-03-01 00:00:00