Abstract:
:A method has been devised for loading chloroplast inner envelope vesicles prepared from pea (Pisum sativum L. var Progress No. 9) or spinach (Spinacia oleracea L.) with 8-hydroxypyrene-1,3,6-trisulfonate (pyranine), a membrane impermeant, fluorescent pH indicator. Two known proton-linked transport activities of the inner envelope, glycolate/H(+) co-transport and phosphate/phosphoglycerate exchange have been shown to cause quenching of the internal pyranine fluorescence. This represents the first demonstration that these vesicles are sealed and competent for transport measurements. The technique, as it now stands, is essentially qualitative. It does, however, offer advantages over transport measurements with intact chloroplasts, for example compatibility with rapid mixing techniques and accessibility of the transport proteins to antibodies.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Howitz KT,McCarty REdoi
10.1104/pp.86.4.999subject
Has Abstractpub_date
1988-04-01 00:00:00pages
999-1001issue
4eissn
0032-0889issn
1532-2548journal_volume
86pub_type
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