Guard Cells Integrate Light and Temperature Signals to Control Stomatal Aperture.

Abstract:

:High temperature promotes guard cell expansion, which opens stomatal pores to facilitate leaf cooling. How the high-temperature signal is perceived and transmitted to regulate stomatal aperture is, however, unknown. Here, we used a reverse-genetics approach to understand high temperature-mediated stomatal opening in Arabidopsis (Arabidopsis thaliana). Our findings reveal that high temperature-induced guard cell movement requires components involved in blue light-mediated stomatal opening, suggesting cross talk between light and temperature signaling pathways. The molecular players involved include phototropin photoreceptors, plasma membrane H+-ATPases, and multiple members of the 14-3-3 protein family. We further show that phototropin-deficient mutants display impaired rosette evapotranspiration and leaf cooling at high temperatures. Blocking the interaction of 14-3-3 proteins with their client proteins severely impairs high temperature-induced stomatal opening but has no effect on the induction of heat-sensitive guard cell transcripts, supporting the existence of an additional intracellular high-temperature response pathway in plants.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Kostaki KI,Coupel-Ledru A,Bonnell VC,Gustavsson M,Sun P,McLaughlin FJ,Fraser DP,McLachlan DH,Hetherington AM,Dodd AN,Franklin KA

doi

10.1104/pp.19.01528

subject

Has Abstract

pub_date

2020-03-01 00:00:00

pages

1404-1419

issue

3

eissn

0032-0889

issn

1532-2548

pii

pp.19.01528

journal_volume

182

pub_type

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