Characterization of a new antifungal chitin-binding peptide from sugar beet leaves.

Abstract:

:The intercellular washing fluid (IWF) from leaves of sugar beet (Beta vulgaris L.) contains a number of proteins exhibiting in vitro antifungal activity against the devastating leaf pathogen Cercospora beticola (Sacc.). Among these, a potent antifungal peptide, designated IWF4, was identified. The 30-amino-acid residue sequence of IWF4 is rich in cysteines (6) and glycines (7) and has a highly basic isoelectric point. IWF4 shows homology to the chitin-binding (hevein) domain of chitin-binding proteins, e.g. class I and IV chitinases. Accordingly, IWF4 has a strong affinity to chitin. Notably, it binds chitin more strongly than the chitin-binding chitinases. A full-length IWF4 cDNA clone was obtained that codes for a preproprotein of 76 amino acids containing an N-terminal putative signal peptide of 21 residues, followed by the mature IWF4 peptide of 30 residues, and an acidic C-terminal extension of 25 residues. IWF4 mRNA is expressed in the aerial parts of the plant only, with a constitutive expression in young and mature leaves and in young flowers. No induced expression of IWF4 protein or mRNA was detected during infection with C. beticola or after treatment with 2,6-dichloroisonicotinic acid, a well-known inducer of resistance in plants.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Nielsen KK,Nielsen JE,Madrid SM,Mikkelsen JD

doi

10.1104/pp.113.1.83

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

83-91

issue

1

eissn

0032-0889

issn

1532-2548

pii

113/1/83

journal_volume

113

pub_type

杂志文章
  • Identification of negative cis-acting elements in response to copper in the chloroplastic iron superoxide dismutase gene of the moss Barbula unguiculata.

    abstract::Superoxide dismutases (SODs) are ubiquitous metalloenzymes that catalyze the dismutation of superoxide radicals. Chloroplasts have two isozymes, copper/zinc SOD (Cu/ZnSOD) and iron SOD (FeSOD), encoded by nuclear genes. Because bryophytes are considered as the earliest land plants, they are one of the most interesting...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.114868

    authors: Nagae M,Nakata M,Takahashi Y

    更新日期:2008-04-01 00:00:00

  • Effects of decenylsuccinic Acid on p uptake and translocation by barley and winter wheat.

    abstract::After 3 days of exposure to 10(-3) and 10(-4) M decenylsuccinic acid, winter wheat plants wilted and died. Decenylsuccinate at 10(-3) M inhibited (32)P uptake by barley roots and wheat roots and resulted in significant (P

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.45.1.1

    authors: Green DG,Ferguson WS,Warder FG

    更新日期:1970-01-01 00:00:00

  • Expression of tobacco carbonic anhydrase in the C4 dicot flaveria bidentis leads to increased leakiness of the bundle sheath and a defective CO2-concentrating mechanism

    abstract::Flaveria bidentis (L.) Kuntze, a C4 dicot, was genetically transformed with a construct encoding the mature form of tobacco (Nicotiana tabacum L.) carbonic anhydrase (CA) under the control of a strong constitutive promoter. Expression of the tobacco CA was detected in transformant whole-leaf and bundle-sheath cell (bs...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.3.1071

    authors: Ludwig M,von Caemmerer S,Dean Price G,Badger MR,Furbank RT

    更新日期:1998-07-01 00:00:00

  • Identification of Arabidopsis ethylene-responsive element binding factors with distinct induction kinetics after pathogen infection.

    abstract::Ethylene-responsive element binding factors (ERF) proteins are plant-specific transcription factors, many of which have been linked to stress responses. We have identified four Arabidopsis ERF genes whose expression was specifically induced by avirulent and virulent strains of the bacterial pathogen Pseudomonas syring...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010862

    authors: Oñate-Sánchez L,Singh KB

    更新日期:2002-04-01 00:00:00

  • Ubiquitin Specific Protease 15 Has an Important Role in Regulating Grain Width and Size in Rice.

    abstract::Ubiquitination and deubiquitination are reversible processes that play crucial roles in regulating organ size in plants. However, information linking deubiquitination and seed size in rice (Oryza sativa) is limited. Here, we characterized a dominant large-grain mutant, large grain1-D (lg1-D), with a 30.8% increase in ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00065

    authors: Shi C,Ren Y,Liu L,Wang F,Zhang H,Tian P,Pan T,Wang Y,Jing R,Liu T,Wu F,Lin Q,Lei C,Zhang X,Zhu S,Guo X,Wang J,Zhao Z,Wang J,Zhai H,Cheng Z,Wan J

    更新日期:2019-05-01 00:00:00

  • Abscission: the role of RNA synthesis.

    abstract::Ethylene stimulated the incorporation of (32)P into RNA in the abscission zone of bean explants (Phaseolus vulgaris L. var. Red Kidney). The enhancement was observed in all fractions separated by methylated albumin kieselguhr column chromatography, although the magnitude of the increase was not the same for each fract...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.42.8.1094

    authors: Holm RE,Abeles FB

    更新日期:1967-08-01 00:00:00

  • Mode of action of a herbicide : johnsongrass and methanearsonic Acid.

    abstract::Johnsongrass (Sorghum halepense (L.) Pers.) is sensitive to methanearsonate, foliar application resulting in a topkill. Investigation of the pattern of photosynthesis by radioautography revealed an accumulation of malate in methanearsonate-treated leaves. Accumulation of malate was attributed to an inhibition of NADP(...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.2.235

    authors: Knowles FC,Benson AA

    更新日期:1983-02-01 00:00:00

  • Photosynthesis in Eurasian Watermilfoil (Myriophyllum spicatum L.).

    abstract::Gas exchange of Eurasian watermilfoil (Myriophyllum spicatum L.) indicated a near-zero CO(2) compensation point and a high temperature optimum for photosynthesis. These properties are characteristic of plants fixing CO(2) by a beta-carboxylation mechanism. Operation of the Calvin cycle was shown and no evidence for be...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.50.1.149

    authors: Stanley RA,Naylor AW

    更新日期:1972-07-01 00:00:00

  • Identification of 1,4-Benzoxazin-3-ones in Maize Extracts by Gas-Liquid Chromatography and Mass Spectrometry.

    abstract::Gas-liquid chromatography-mass spectrometry (GLC-MS) has been used for the separation, detection, and identification of 1,4-benzoxazin-3-ones (hydroxamic acids and lactams) and benzoxazolinones found in maize (Zea mays L.) extracts. Compounds of interest were partitioned into ethyl acetate from aqueous maize seedling ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.63.1.9

    authors: Woodward MD,Corcuera LJ,Schnoes HK,Helgeson JP,Upper CD

    更新日期:1979-01-01 00:00:00

  • Appearance of an Alternate Pathway Cyanide-resistant during Germination of Seeds of Cicer arietinum.

    abstract::The combined action of the inhibitors antimycin A and cyanide with benzohydroxamic acid indicates the presence of a cyanide-resistant pathway of respiration in chick pea (Cicer arietinum L.) seeds. The appearance of this pathway takes place during germination. During the first 12 hours of germination, the respiration ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.60.4.524

    authors: Burguillo Pde L,Nicolás G

    更新日期:1977-10-01 00:00:00

  • The effect of exogenous abscisic acid on stomatal development, stomatal mechanics, and leaf gas exchange in Tradescantia virginiana.

    abstract::Gas exchange parameters and stomatal physical properties were measured in Tradescantia virginiana plants grown under well-watered conditions and treated daily with either distilled water (control) or 3.0 mM abscisic acid (ABA). Photosynthetic capacity (CO(2) assimilation rate for any given leaf intercellular CO(2) con...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.2.935

    authors: Franks PJ,Farquhar GD

    更新日期:2001-02-01 00:00:00

  • A CCCH-type zinc finger nucleic acid-binding protein quantitatively confers resistance against rice bacterial blight disease.

    abstract::Bacterial blight is a devastating disease of rice (Oryza sativa) caused by Xanthomonas oryzae pv oryzae (Xoo). Zinc finger proteins harboring the motif with three conserved cysteine residues and one histidine residue (CCCH) belong to a large family. Although at least 67 CCCH-type zinc finger protein genes have been id...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.191379

    authors: Deng H,Liu H,Li X,Xiao J,Wang S

    更新日期:2012-02-01 00:00:00

  • Deep Supercooling in Most Tissues of Wintering Sasa senanensis and Its Mechanism in Leaf Blade Tissues.

    abstract::Cold hardiness of leaf blades, leaf sheaths, culms, rhizomes, and leaf buds in wintering Sasa senanensis (Fr. et Sav.) Rehder, a dwarf bamboo, was studied paying special attention to the types of resistance mechanisms which were determined with differential thermal analysis. Coincidence of LT(25) (lethal temperature a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.1.196

    authors: Ishikawa M

    更新日期:1984-05-01 00:00:00

  • An Arabidopsis prenylated Rab acceptor 1 isoform, AtPRA1.B6, displays differential inhibitory effects on anterograde trafficking of proteins at the endoplasmic reticulum.

    abstract::Prenylated Rab acceptors (PRAs), members of the Ypt-interacting protein family of small membrane proteins, are thought to aid the targeting of prenylated Rabs to their respective endomembrane compartments. In plants, the Arabidopsis (Arabidopsis thaliana) PRA1 family contains 19 members that display varying degrees of...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.180810

    authors: Lee MH,Jung C,Lee J,Kim SY,Lee Y,Hwang I

    更新日期:2011-10-01 00:00:00

  • Effect of vanadate on rhythmic leaflet movement in albizzia julibrissin.

    abstract::Vanadate (Na(3)VO(4)) selectively and reversibly affects the rhythmic movement of Albizzia julibrissin leaflets. Leaflets floated on 1 millimolar vanadate open at the same rate or more rapidly than controls, but closure is inhibited. After 6 to 24 hours incubation, the inhibition can be reversed by a 24-to 48-hour per...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.64.5.905

    authors: Saxe H,Satter RL

    更新日期:1979-11-01 00:00:00

  • Strigolactone acts downstream of auxin to regulate bud outgrowth in pea and Arabidopsis.

    abstract::During the last century, two key hypotheses have been proposed to explain apical dominance in plants: auxin promotes the production of a second messenger that moves up into buds to repress their outgrowth, and auxin saturation in the stem inhibits auxin transport from buds, thereby inhibiting bud outgrowth. The recent...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.134783

    authors: Brewer PB,Dun EA,Ferguson BJ,Rameau C,Beveridge CA

    更新日期:2009-05-01 00:00:00

  • Essential role of urease in germination of nitrogen-limited Arabidopsis thaliana seeds.

    abstract::In Arabidopsis thaliana, urease transcript levels increased sharply between 2 and 4 d after germination (DAG) and were maintained at maximal levels until at least 8 DAG. Seed urease specific activity declined upon germination but began to increase in seedlings 2 DAG, reaching approximately 75% of seed activity by 8 DA...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.4.1097

    authors: Zonia LE,Stebbins NE,Polacco JC

    更新日期:1995-04-01 00:00:00

  • Dead or Alive? Using Membrane Failure and Chlorophyll a Fluorescence to Predict Plant Mortality from Drought.

    abstract::Climate models predict widespread increases in both drought intensity and duration in the next decades. Although water deficiency is a significant determinant of plant survival, limited understanding of plant responses to extreme drought impedes forecasts of both forest and crop productivity under increasing aridity. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00581

    authors: Guadagno CR,Ewers BE,Speckman HN,Aston TL,Huhn BJ,DeVore SB,Ladwig JT,Strawn RN,Weinig C

    更新日期:2017-09-01 00:00:00

  • Localization of [gamma]-Glutamylcysteine Synthetase and Glutathione Synthetase Activity in Maize Seedlings.

    abstract::Fresh weight, protein, cysteine, [gamma]-glutamylcysteine, glutathione, and the extractable activity of the enzymes of glutathione biosynthesis, [gamma]-glutamylcysteine synthetase (EC 6.3.2.2) and glutathione synthetase (EC 6.3.2.3), were measured in roots, scutella, endosperms, and shoots of 3-, 7-, and 11-d-old mai...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.2.561

    authors: Ruegsegger A,Brunold C

    更新日期:1993-02-01 00:00:00

  • Regions of differential cell elongation and mitosis, and root meristem morphology in different tissues of geotropically stimulated maize root apices.

    abstract::We examined cell length, mitosis, and root meristem "cuticle" in different tissues of geostimulated, red light-exposed primary roots of corn (Zea Mays, Wisconsin hybrid 64A x 22R). The examination was done at 15-minute intervals for a period of 240 minutes. Differences in cell elongation between the upper and lower si...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.1.7

    authors: Shen-Miller J,McNitt RE,Wojciechowski M

    更新日期:1978-01-01 00:00:00

  • Oxygen-18 and deuterium labeling studies of choline oxidation by spinach and sugar beet.

    abstract::Chenopods synthesize betaine by a two-step oxidation of choline: choline --> betaine aldehyde --> betaine. The pathway is chloroplastic; the first step has been shown in isolated spinach (Spinacia oleracea L.) chloroplasts to be O(2)- and light-dependent, the role of light being to provide reducing power (P Weigel, EA...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.88.3.695

    authors: Lerma C,Hanson AD,Rhodes D

    更新日期:1988-11-01 00:00:00

  • An improved green fluorescent protein gene as a vital marker in plants.

    abstract::A synthetic green fluorescent protein (GFP) gene (pgfp) was constructed to improve GFP expression in plants. Corn and tobacco protoplast transient assays showed that pgfp gave about 20-fold brighter fluorescence than the wild-type gene (gfp). Replacement of the serine at position 65 with a threonine (S65Tpgfp) or a cy...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.3.893

    authors: Pang SZ,DeBoer DL,Wan Y,Ye G,Layton JG,Neher MK,Armstrong CL,Fry JE,Hinchee MA,Fromm ME

    更新日期:1996-11-01 00:00:00

  • On the Factors Which Determine Massive beta-Carotene Accumulation in the Halotolerant Alga Dunaliella bardawil.

    abstract::Dunaliella bardawil, a beta-carotene-accumulating halotolerant alga, has been analyzed for the effect of various growth conditions on its pigment content, and compared with Dunaliella salina, a beta-carotene nonaccumulating species. In D. bardawil, increasing light intensity and light period or inhibiting growth by va...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.3.593

    authors: Ben-Amotz A,Avron M

    更新日期:1983-07-01 00:00:00

  • Phytochrome induces rapid PIF5 phosphorylation and degradation in response to red-light activation.

    abstract::The phytochrome (phy) family of sensory photoreceptors (phyA-phyE in Arabidopsis thaliana) induces changes in target-gene expression upon light-induced translocation to the nucleus, where certain members interact with selected members of the constitutively nuclear basic helix-loop-helix transcription factor family, su...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.105601

    authors: Shen Y,Khanna R,Carle CM,Quail PH

    更新日期:2007-11-01 00:00:00

  • A brassinosteroid-hypersensitive mutant of BAK1 indicates that a convergence of photomorphogenic and hormonal signaling modulates phototropism.

    abstract::The phototropic response of Arabidopsis (Arabidopsis thaliana) is induced by the phototropin photoreceptors and modulated by the cryptochrome and phytochrome photoreceptors. Downstream of these photoreceptors, asymmetric lateral redistribution of auxin underlies the differential growth, which results in phototropism. ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.064444

    authors: Whippo CW,Hangarter RP

    更新日期:2005-09-01 00:00:00

  • Cold-regulated cereal chloroplast late embryogenesis abundant-like proteins. Molecular characterization and functional analyses.

    abstract::Cold acclimation and freezing tolerance are the result of complex interaction between low temperature, light, and photosystem II (PSII) excitation pressure. Previous results have shown that expression of the Wcs19 gene is correlated with PSII excitation pressure measured in vivo as the relative reduction state of PSII...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.001925

    authors: NDong C,Danyluk J,Wilson KE,Pocock T,Huner NP,Sarhan F

    更新日期:2002-07-01 00:00:00

  • Evidence That IAA Conjugates Are Slow-Release Sources of Free IAA in Plant Tissues.

    abstract::Evidence that indoleacetic acid (IAA) conjugates are metabolized via enzyme-catalyzed hydrolysis to free IAA and that their biological activities are related to the rates at which they are hydrolyzed by the tissue is presented. These conclusions are based on the following observations. Slow but continuous decarboxylat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.6.1424

    authors: Hangarter RP,Good NE

    更新日期:1981-12-01 00:00:00

  • Kok effect and the quantum yield of photosynthesis : light partially inhibits dark respiration.

    abstract::The linear response of photosynthesis to light at low photon flux densities is known to change abruptly in the vicinity of the light compensation point so that the quantum yield seems to decrease as radiation increases. We studied this ;Kok effect' in attached sunflower (Helianthus annuus L. cv IS894) leaves using gas...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.1.95

    authors: Sharp RE,Matthews MA,Boyer JS

    更新日期:1984-05-01 00:00:00

  • Molecular and functional analyses support a role of Ornithine-{delta}-aminotransferase in the provision of glutamate for glutamine biosynthesis during pine germination.

    abstract::We report the molecular characterization and functional analysis of a gene (PsdeltaOAT) from Scots pine (Pinus sylvestris) encoding Orn-delta-aminotransferase (delta-OAT; EC 2.6.1.13), an enzyme of arginine metabolism. The deduced amino acid sequence contains a putative N-terminal signal peptide for mitochondrial targ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.122853

    authors: Cañas RA,Villalobos DP,Díaz-Moreno SM,Cánovas FM,Cantón FR

    更新日期:2008-09-01 00:00:00

  • Cucumber seedling indoleacetaldehyde oxidase.

    abstract::Extracts of light-grown Cucumis sativus L. seedlings catalyzed the oxidation of indole-3-acetaldehyde to indole-3-acetic acid. No added cofactors were required. Inhibitor studies indicated that the enzyme is a metalloflavoprotein. While indole-3-aldehyde, benzaldehyde, and phenylacetaldehyde partially inhibited the ox...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.1.107

    authors: Bower PJ,Brown HM,Purves WK

    更新日期:1978-01-01 00:00:00