Expression of tobacco carbonic anhydrase in the C4 dicot flaveria bidentis leads to increased leakiness of the bundle sheath and a defective CO2-concentrating mechanism


:Flaveria bidentis (L.) Kuntze, a C4 dicot, was genetically transformed with a construct encoding the mature form of tobacco (Nicotiana tabacum L.) carbonic anhydrase (CA) under the control of a strong constitutive promoter. Expression of the tobacco CA was detected in transformant whole-leaf and bundle-sheath cell (bsc) extracts by immunoblot analysis. Whole-leaf extracts from two CA-transformed lines demonstrated 10% to 50% more CA activity on a ribulose-1,5-bisphosphate carboxylase/oxygenase-site basis than the extracts from transformed, nonexpressing control plants, whereas 3 to 5 times more activity was measured in CA transformant bsc extracts. This increased CA activity resulted in plants with moderately reduced rates of CO2 assimilation (A) and an appreciable increase in C isotope discrimination compared with the controls. With increasing O2 concentrations up to 40% (v/v), a greater inhibition of A was found for transformants than for wild-type plants; however, the quantum yield of photosystem II did not differ appreciably between these two groups over the O2 levels tested. The quantum yield of photosystem II-to-A ratio suggested that at higher O2 concentrations, the transformants had increased rates of photorespiration. Thus, the expression of active tobacco CA in the cytosol of F. bidentis bsc and mesophyll cells perturbed the C4 CO2-concentrating mechanism by increasing the permeability of the bsc to inorganic C and, thereby, decreasing the availability of CO2 for photosynthetic assimilation by ribulose-1,5-bisphosphate carboxylase/oxygenase.


Plant Physiol


Plant physiology


Ludwig M,von Caemmerer S,Dean Price G,Badger MR,Furbank RT




Has Abstract


1998-07-01 00:00:00












  • Effect of Glucose and CO(2) on Nitrate Uptake and Coupled OH Flux in Ankistrodesmus braunii.

    abstract::In Ankistrodesmus braunii, in the absence of CO(2), i.e. in CO(2)-free air or N(2), photosynthetic nitrate uptake and nitrate reduction were inhibited, especially at low pH. Under such conditions, glucose stimulated nitrate uptake and reduction to almost the same level in the pH range between 6 and 8.5. CO(2) at 0.03%...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Eisele R,Ullrich WR

    更新日期:1977-01-01 00:00:00

  • Regulation of beta-Glucan Synthetase Activity by Auxin in Pea Stem Tissue: II. Metabolic Requirements.

    abstract::The 2- to 4-fold rise in particle-bound beta-glucan synthetase (uridine diphosphate-glucose: beta-1, 4-glucan glucosyltransferase) activity that can be induced by indoleacetic acid in pea stem tissue is not prevented by concentrations of actinomycin D or cycloheximide that inhibit growth and macromolecule synthesis. T...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Ray PM

    更新日期:1973-04-01 00:00:00

  • Polysome Formation in Light-controlled Dormancy.

    abstract::Lettuce (Lactuca sativa) seeds var. Grand Rapids could be maintained many weeks in the dark without germination. Following illumination with white light, a gradual increase in polyribosome population up to the time of germination was demonstrated by sucrose gradient centrifugation. Polysomes could not be detected in i...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Mitchell RC,Villiers TA

    更新日期:1972-12-01 00:00:00

  • Photosynthesis in Eurasian Watermilfoil (Myriophyllum spicatum L.).

    abstract::Gas exchange of Eurasian watermilfoil (Myriophyllum spicatum L.) indicated a near-zero CO(2) compensation point and a high temperature optimum for photosynthesis. These properties are characteristic of plants fixing CO(2) by a beta-carboxylation mechanism. Operation of the Calvin cycle was shown and no evidence for be...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Stanley RA,Naylor AW

    更新日期:1972-07-01 00:00:00

  • Manipulation of galactolipid Fatty Acid composition with substituted pyridazinones.

    abstract::The fatty acid composition of the major lipids of the chloroplast membranes, the mono- and digalactosyl diglycerides, can be definably altered with various substituted pyridazinones. Galactolipid fatty acid composition of wheat (Triticum aestivum L.) can be altered so that there is a decrease in linolenic acid accompa...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: John JB

    更新日期:1976-01-01 00:00:00

  • Isolation of Intact and Functional Chloroplasts from Mesophyll and Bundle Sheath Protoplasts of the C(4) Plant Panicum miliaceum.

    abstract::A procedure is described for isolating and purifying mesophyll protoplasts and bundle sheath protoplasts of the C(4) plant Panicum miliaceum. Following enzymic digestion of leaf tissue, mesophyll protoplasts and bundle sheath protoplasts are released and purified by density centrifugation. The lower density of mesophy...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Edwards GE

    更新日期:1979-05-01 00:00:00

  • Distribution and Activity of the Plasma Membrane H+-ATPase in Mimosa pudica L. in Relation to Ionic Fluxes and Leaf Movements.

    abstract::Plasma membrane H+-ATPase was immunolocalized in several cell types of the sensitive plant Mimosa pudica L., and transmembrane potentials were measured on cortical cells. In comparison with the nonspecialized cortical cells of the petiole or stem, the proton pump was highly expressed in motor cells. These immunologica...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Fleurat-Lessard P,Bouche-Pillon S,Leloup C,Bonnemain JL

    更新日期:1997-03-01 00:00:00

  • Potassium-dependent changes in the expression of membrane-associated proteins in barley roots : I. Correlations with k(rb) influx and root k concentration.

    abstract::Barley (Hordeum vulgare L. cv Halcyon) seedlings which had been grown in full strength complete inorganic nutrient media (containing 6 millimolar K(+)) had high internal K(+) concentrations and low values of K(+) ((86)Rb(+)) influx when influx was measured from solutions containing 100 micromolar K(+). Transfer of the...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Fernando M,Kulpa J,Siddiqi MY,Glass AD

    更新日期:1990-04-01 00:00:00

  • Stomatal Responses to Light and Drought Stress in Variegated Leaves of Hedera helix.

    abstract::Direct and indirect mechanisms underlying the light response of stomata were studied in variegated leaves of the juvenile phase of Hedera helix L. Dose response curves of leaf conductance were measured with blue and red light in leaves kept in normal or in an inverted position. In the green portions of the leaves, the...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Aphalo PJ,Sánchez RA

    更新日期:1986-07-01 00:00:00

  • Enzymes of starch metabolism in the developing rice grain.

    abstract::The levels of starch, soluble sugars, protein, and enzymes involved in starch metabolism-alpha-amylase, beta-amylase, phosphorylase, Q-enzyme, R-enzyme, and starch synthetase -were assayed in dehulled developing rice grains (Oryzasativa L., variety IR8). Phosphorylase, Q-enzyme, and R-enzyme had peak activities 10 day...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Baun LC,Palmiano EP,Perez CM,Juliano BO

    更新日期:1970-09-01 00:00:00

  • The relationship of the peroxidative indoleacetic Acid oxidase system to in vivo ethylene synthesis in cotton.

    abstract::Since peroxidase and manganese have been implicated in both auxin destruction and ethylene production, the effect of auxins and high tissue levels of manganese on the peroxidative indoleacetic acid oxidase system and the internal level of ethylene was determined in cotton (Gossypium hirsutum L. cv. Watson GL-7). The h...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Fowler JL,Morgan PW

    更新日期:1972-04-01 00:00:00

  • Photosynthetic Carbon Metabolism in Photoautotrophic Cell Suspension Cultures Grown at Low and High CO(2).

    abstract::Photosynthetic carbon metabolism was characterized in four photoautotrophic cell suspension cultures. There was no apparent difference between two soybean (Glycine max) and one cotton (Gossypium hirsutum) cell line which required 5% CO(2) for growth, and a unique cotton cell line that grows at ambient CO(2) (660 micro...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Roeske CA,Widholm JM,Ogren WL

    更新日期:1989-12-01 00:00:00

  • Candidate genes and quantitative trait loci affecting fruit ascorbic acid content in three tomato populations.

    abstract::Fresh fruit and vegetables are a major source of ascorbic acid (vitamin C), an important antioxidant for the human diet and also for plants. Ascorbic acid content in fruit exhibits a quantitative inheritance. Quantitative trait loci (QTL) for ascorbic acid content have been mapped in three tomato populations derived f...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Stevens R,Buret M,Duffé P,Garchery C,Baldet P,Rothan C,Causse M

    更新日期:2007-04-01 00:00:00

  • Light Energy Dissipation under Water Stress Conditions: Contribution of Reassimilation and Evidence for Additional Processes.

    abstract::Using (14)CO(2) gas exchange and metabolite analyses, stomatal as well as total internal CO(2) uptake and evolution were estimated. Pulse modulated fluorescence was measured during induction and steady state of photosynthesis. Leaf water potential of Digitalis lanata EHRH. plants decreased to -2.5 megapascals after wi...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Stuhlfauth T,Scheuermann R,Fock HP

    更新日期:1990-04-01 00:00:00

  • Linear beta-1,3 glucans are elicitors of defense responses in tobacco.

    abstract::Laminarin, a linear beta-1,3 glucan (mean degree of polymerization of 33) was extracted and purified from the brown alga Laminaria digitata. Its elicitor activity on tobacco (Nicotiana tabacum) was compared to that of oligogalacturonides with a mean degree of polymerization of 10. The two oligosaccharides were perceiv...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Klarzynski O,Plesse B,Joubert JM,Yvin JC,Kopp M,Kloareg B,Fritig B

    更新日期:2000-11-01 00:00:00

  • Photosynthesis, Rubisco Activity and Amount, and Their Regulation by Transcription in Senescing Soybean Leaves.

    abstract::Senescence is a phase of leaf ontogeny marked by declining photosynthetic activity that, in soybean (Glycine max [L.] Merr.), is paralleled by a decline in chloroplast function. Soybean leaves have different patterns of decline in photosynthetic capacity and chloroplast function associated with nodal position and sink...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Jiang CZ,Rodermel SR,Shibles RM

    更新日期:1993-01-01 00:00:00

  • Structure and expression of chloroplast-localized porphobilinogen deaminase from pea (Pisum sativum L.) isolated by redundant polymerase chain reaction.

    abstract::Porphobilinogen (PBG) deaminase catalyzes the polymerization of four PBG monopyrrole units into the linear tetrapyrrole hydroxymethylbilane necessary for the formation of chlorophyll and heme in plant cells. Degenerate oligonucleotide primers were designed based on amino acid sequence data (generated by mass spectrome...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Witty M,Wallace-Cook AD,Albrecht H,Spano AJ,Michel H,Shabanowitz J,Hunt DF,Timko MP,Smith AG

    更新日期:1993-09-01 00:00:00

  • Characterization of a meta-Fluorotyrosine-Tolerant Cell Culture of Eschscholtzia californica Cham.

    abstract::A cell line of Eschscholtzia californica selected for meta-fluorotyrosine (MFT) tolerance was found to have 10-fold increased levels of phenylalanine and tyrosine compared to the parent line, while most other amino acids were only increased 2-fold. Tracer experiments with shikimic acid in the presence of MFT showed th...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Berlin J,van Huystee RB,Witte L,Bender J,Jäger HJ,Wray V

    更新日期:1989-04-01 00:00:00

  • Reductions of Rubisco activase by antisense RNA in the C4 plant Flaveria bidentis reduces Rubisco carbamylation and leaf photosynthesis.

    abstract::To function, the catalytic sites of Rubisco (EC need to be activated by the reversible carbamylation of a lysine residue within the sites followed by rapid binding of magnesium. The activation of Rubisco in vivo requires the presence of the regulatory protein Rubisco activase. This enzyme is thought to aid t...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: von Caemmerer S,Hendrickson L,Quinn V,Vella N,Millgate AG,Furbank RT

    更新日期:2005-02-01 00:00:00

  • Effect of Temperature, CO(2) Concentration, and Light Intensity on Oxygen Inhibition of Photosynthesis in Wheat Leaves.

    abstract::The effect of 21% O(2) and 3% O(2) on the CO(2) exchange of detached wheat leaves was measured in a closed system with an infrared carbon dioxide analyzer. Temperature was varied between 2 degrees and 43 degrees , CO(2) concentration between 0.000% and 0.050% and light intensity between 40 ft-c and 1000 ft-c. In most ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Jolliffe PA,Tregunna EB

    更新日期:1968-06-01 00:00:00

  • Cytokinin and inhibitor activities in the avocado fruit mesocarp.

    abstract::No cytokinin activity was found in methanolic mesocarp extracts after purification with petroleum ether and ethyl acetate. Cytokinin activity did appear, however, in aqueous fraction after acid hydrolysis or passage through Dowex 50 (H(+)) ion exchange columns. The level of this activity, the fruit growth rate, and th...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Gazit S,Blumenfeld A

    更新日期:1970-08-01 00:00:00

  • The Ca(2+) status of the endoplasmic reticulum is altered by induction of calreticulin expression in transgenic plants.

    abstract::To investigate the endoplasmic reticulum (ER) Ca(2+) stores in plant cells, we generated tobacco (Nicotiana tabacum; NT1) suspension cells and Arabidopsis plants with altered levels of calreticulin (CRT), an ER-localized Ca(2+)-binding protein. NT1 cells and Arabidopsis plants were transformed with a maize (Zea mays) ...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Persson S,Wyatt SE,Love J,Thompson WF,Robertson D,Boss WF

    更新日期:2001-07-01 00:00:00

  • Oxygen Uptake and Photosynthesis of the Red Macroalga, Chondrus crispus, in Seawater: Effects of Oxygen Concentration.

    abstract::With an experimental system developed for aquatic plants using the mass spectrometry technique and infrared gas analysis of CO(2), we studied the responses to various O(2) concentrations of gas exchanges with the red macroalga Chondrus crispus S. The results were as follows. (a) Irrespective of the CO(2) concentration...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Brechignac F,Andre M

    更新日期:1985-07-01 00:00:00

  • Primary structures of Arabidopsis calmodulin isoforms deduced from the sequences of cDNA clones.

    abstract::Complementary DNA (cDNA) clones encoding calmodulin isoforms were isolated from an Arabidopsis leaf lambdagt10 library by screening with cloned barley calmodulin cDNA probes. Two cDNAs, one a 626-base pair partial-length clone (ACaM-1) and one a 1400-base pair full-length clone (ACaM-2), encode calmodulin polypeptides...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Ling V,Perera I,Zielinski RE

    更新日期:1991-08-01 00:00:00

  • Stachyose Synthesis in Source Leaf Tissues of the CAM Plant Xerosicyos danguyi H. Humb.

    abstract::Leaf tissues from Xerosicyos danguyi H. Humb., a succulent member of the Cucurbitaceae, were found to possess both galactinol synthase activity and the capacity for photosynthetic production of stachyose, the phloem transport oligosaccharide common to other nonsucculent cucurbits. The amounts of stachyose isolated fro...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Madore MA,Mitchell DE,Boyd CM

    更新日期:1988-07-01 00:00:00

  • Concomitant determination of folar nitrogen loss, net carbon dioxide uptake, and transpiration.

    abstract::A closed system was designed for concomitant determination of net CO(2) uptake, transpiration, and foliar nitrogen (N) loss in soybeans (Glycine max [L.] Merr.). The CO(2) uptake was monitored by infrared analysis of system air removed periodically in aliquots. Leaf vapors were trapped in calibrated tubes at Dry Ice t...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Weiland RT,Stutte CA

    更新日期:1980-02-01 00:00:00

  • Polar Calcium Flux in Sunflower Hypocotyl Segments : II. The Effect of Segment Orientation, Growth, and Respiration.

    abstract::Calcium flux in sunflower (Helianthus annuus L. cv Russian mammoth) hypocotyl was measured with a Ca(2+) electrode as the increase or decrease in Ca(2+) in an aqueous solution (10 micromolar CaCl(2)) in contact with either the basal or apical end of 20 millimeter segments. Ca(2+) efflux was significantly higher at the...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: de Guzman CC,Dela Fuente RK

    更新日期:1986-06-01 00:00:00

  • Three-Dimensional Analysis of Chloroplast Structures Associated with Virus Infection.

    abstract::Chloroplasts are multifunctional organelles whose morphology is affected by environmental stresses. Although the three-dimensional (3D) architecture of thylakoid membranes has been reported previously, a 3D visualization of chloroplast under stress has not been explored. In this work, we used a positive-strand RNA ((+...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Jin X,Jiang Z,Zhang K,Wang P,Cao X,Yue N,Wang X,Zhang X,Li Y,Li D,Kang BH,Zhang Y

    更新日期:2018-01-01 00:00:00

  • The Role of ATP in Mechanically Stimulated Rapid Closure of the Venus's Flytrap.

    abstract::When the midribs of untreated traps of Dionaea muscipula are frozen in liquid nitrogen after rapid closure, they contain significantly less ATP than those frozen before closure. Exogenous ATP causes a significant increase in the rate of mechanically stimulated trap closure. Illuminated traps close faster than those ke...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Jaffe MJ

    更新日期:1973-01-01 00:00:00

  • Characterization of Plasma Membrane-associated Adenosine Triphosphase Activity of Oat Roots.

    abstract::ATPase activity of plasma membranes isolated from oat (Avena sativa L. cv. Goodfield) roots was activated by divalent cations (Mg(2+) = Mn(2+) > Zn(2+) > Fe(2+) > Ca(2+)) and further stimulated by KCl and a variety of monovalent salts, both inorganic and organic. The enzyme exhibited greater specificity for cations th...

    journal_title:Plant physiology

    pub_type: 杂志文章


    authors: Leonard RT,Hodges TK

    更新日期:1973-07-01 00:00:00