Abstract:
:The response of cortical microtubules to low temperature and freezing was assessed for root tips of cold-acclimated and non-acclimated winter rye (Secale cereale L. cv Puma) seedlings using indirect immunofluorescence microscopy with antitubulin antibodies. Roots cooled to 0 or -3 degrees C were fixed for immunofluorescence microscopy at these temperatures or after an additional hour at 4 degrees C. Typical arrays of cortical microtubules were present in root-tip cells of seedlings exposed to the cold-acclimation treatment of 4 degrees C for 2 days. Microtubules in these cold-acclimated cells were more easily depolymerized by a 0 degrees C treatment than microtubules in root-tip cells of nonacclimated, 22 degrees C-grown seedlings. Microtubules were still present in some cells of both nonacclimated and cold-acclimated roots at 0 and -3 degrees C; however, the number of microtubules in these cells was lower than in controls. Microtubules remaining during the -3 degrees C freeze were shorter than microtubules in unfrozen control cells. Repolymerization of microtubules after both the 0 and -3 degrees C treatments occurred within 1 h. Root tips of nonacclimated seedlings had an LT-50 of -9 degrees C. Cold acclimation lowered this value to -14 degrees C. Treatment of 22 degrees C-grown seedlings for 24 h with the microtubule-stabilizing drug taxol caused a decrease in the freezing tolerance of root tips, indicated by a LT-50 of -3 degrees C. Treatment with D-secotaxol, an analog of taxol that was less effective in stabilizing microtubules, did not alter the freezing tolerance. We interpret these data to indicate that a degree of depolymerization of microtubules is necessary for realization of maximum freezing tolerance in root-tip cells of rye.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Kerr GP,Carter JVdoi
10.1104/pp.93.1.77subject
Has Abstractpub_date
1990-05-01 00:00:00pages
77-82issue
1eissn
0032-0889issn
1532-2548journal_volume
93pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::The large ATP synthase gene cluster in spinach (Spinacia oleracea) plastids encodes four of the six chloroplast-encoded ATP synthase subunits. Expression of this cluster was examined to determine its response to light-induced plastid development. Spinach plastid transcripts were isolated from etiolated tissues, etiola...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.3.1164
更新日期:1992-11-01 00:00:00
abstract::The accumulation and reduction of nitrate in the presence of the nitrogen metabolites asparagine (Asn) and glutamine (Gln) and the carbon metabolite sucrose (Suc) were examined in maize (Zea mays L.) seedlings in an attempt to separate their effects on the nitrate uptake system and the nitrate reduction system. After ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.2.583
更新日期:1997-06-01 00:00:00
abstract::Nickel was established as an essential micronutrient for the growth of temperate cereal crops. Grain from barley (Hordeum vulgare L. cv ;Onda'; containing 40 to 80 nanograms of Ni per gram dry weight) grown in solution culture with negligible Ni concentrations (< 30 nanograms of Ni per liter) exhibited greatly reduced...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.3.801
更新日期:1987-11-01 00:00:00
abstract::Proanthocyanidins (PAs) are secondary metabolites that contribute to plant protection and crop quality. Persimmon (Diospyros kaki) has a unique characteristic of accumulating large amounts of PAs, particularly in its fruit. Normal astringent-type and mutant nonastringent-type fruits show different PA accumulation patt...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.111.191205
更新日期:2012-02-01 00:00:00
abstract::The net initial passive flux (J(Ni)) in reconstituted plasma membrane (PM) vesicles from maize (Zea mays) root cells was measured as recently described (P. Pouliquin, J.-P. Grouzis, R. Gibrat ¿1999 Biophys J 76: 360-373). J(Ni) in control liposomes responded to membrane potential or to NO(3)(-) as expected from the Go...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.122.1.265
更新日期:2000-01-01 00:00:00
abstract::The effect of inorganic carbon (Ci) transport and accumulation on photosynthetic electron transport was studied in air-grown cells of the cyanobacterium Synechococcus UTEX 625. When the cells were depleted of Ci, linear photosynthetic electron flow was almost completely inhibited in the presence of the photosystem I (...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.4.1273
更新日期:1997-08-01 00:00:00
abstract::Soybeans (Glycine max) exposed to chronic levels of ozone showed a linear decrease in biomass with increasing concentration. The foliar free sterols increased while the steryl ester, and the steryl glycosides, a minor component, decreased with increasing pollutant concentration. Of the free sterols, stigmasterol showe...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.77.1.245
更新日期:1985-01-01 00:00:00
abstract::We studied the ethylene-insensitive, hypernodulating mutant, sickle (skl), to investigate the interaction of ethylene with auxin transport during root nodulation in Medicago truncatula. Grafting experiments demonstrated that hypernodulation in skl is root controlled. Long distance transport of auxin from shoot to root...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.080093
更新日期:2006-09-01 00:00:00
abstract::Plasma membranes were isolated from oat (Avena sativa) roots by the phase-partitioning method. The membranes were exposed to repeated periods of moderate water-deficit stress, and a water-deficit tolerance was induced (acclimated plants). The plasma membranes of the controls (nonacclimated plants) were characterized b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.4.1136
更新日期:1991-08-01 00:00:00
abstract::Plant cystatins, similar to other defense proteins, include hypervariable, positively selected amino acid sites presumably impacting their biological activity. Using 29 single mutants of the eighth domain of tomato (Solanum lycopersicum) multicystatin, SlCYS8, we assessed here the potential of site-directed mutagenesi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.115741
更新日期:2008-03-01 00:00:00
abstract::Isoamylase-type starch debranching enzymes (ISA) play important roles in starch biosynthesis in chloroplast-containing organisms, as shown by the strict conservation of both catalytically active ISA1 and the noncatalytic homolog ISA2. Functional distinctions exist between species, although they are not understood yet....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.225565
更新日期:2013-11-01 00:00:00
abstract::A radiation-induced mutant of Scotch spearmint (Mentha x gracilis) was shown to produce an essential oil containing principally C3-oxygenated p-menthane monoterpenes that are typical of peppermint, instead of the C6-oxygenated monoterpene family characteristic of spearmint. In vitro measurement of all of the enzymes r...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.96.3.744
更新日期:1991-07-01 00:00:00
abstract::Absorption of selenate and selenite by excised roots of Astragalus Crotalariae, a selenium accumulator, and of A. lentiginosus, a non-accumulator, was favored by CaCl(2) and a pH of 4.0. The uptake of selenate and possibly selenite, is metabolically linked. Roots of a number of Astragalus species were examined, and in...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.1.14
更新日期:1968-01-01 00:00:00
abstract::Plant regeneration is fundamental to basic research and agricultural applications. The regeneration capacity of plants varies largely in different genotypes, but the reason for this variation remains elusive. Here, we identified a novel thioredoxin DCC1 in determining the capacity of shoot regeneration among Arabidops...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00633
更新日期:2018-03-01 00:00:00
abstract::We previously demonstrated that overexpression of the horseradish (Armoracia rusticana) peroxidase prxC1a gene stimulated the growth rate of tobacco (Nicotiana tabacum) plants. Here, the cauliflower mosaic virus 35S::prxC1a construct was introduced into hybrid aspen (Populus sieboldii x Populus grandidentata). The gro...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.102.019794
更新日期:2003-07-01 00:00:00
abstract::Gravity is a critical environmental factor affecting the morphology and functions of organisms on the Earth. Plants sense changes in the gravity vector (gravistimulation) and regulate their growth direction accordingly. In Arabidopsis (Arabidopsis thaliana) seedlings, gravistimulation, achieved by rotating the specime...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.223313
更新日期:2013-10-01 00:00:00
abstract::Thylakoid proteins and their catabolites have been detected in lipid-protein particles isolated from the stroma of intact chloroplasts obtained from primary leaves of 2-week-old bean seedlings (Phaseolus vulgaris L. cv Kinghorn). The lipid-protein particles bear morphological resemblance to plastoglobuli seen in the c...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.4.1547
更新日期:1994-12-01 00:00:00
abstract::Tomato Atypical Receptor Kinase 1 (TARK1) is a pseudokinase required for postinvasion immunity. TARK1 was originally identified as a target of the Xanthomonas euvesicatoria effector protein Xanthomonas outer protein N (XopN), a suppressor of early defense signaling. How TARK1 participates in immune signal transduction...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.01400
更新日期:2020-07-01 00:00:00
abstract::The EGG CELL1 (EC1) gene family of Arabidopsis (Arabidopsis thaliana) comprises five members that are specifically expressed in the egg cell and redundantly control gamete fusion during double fertilization. We investigated the activity of all five EC1 promoters in promoter-deletion studies and identified SUF4 (SUPPRE...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.01024
更新日期:2017-01-01 00:00:00
abstract::In characterizing the enzymes involved in the formation of very long-chain fatty acids (VLCFAs) in the Brassicaceae, we have generated a series of mutants of Arabidopsis thaliana that have reduced VLCFA content. Here we report the characterization of a seed lipid mutant, AS11, which, in comparison to wild type (WT), h...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.1.399
更新日期:1995-05-01 00:00:00
abstract::Oligomeric proanthocyanidins (PAs) composed primarily of epicatechin units accumulate in the seed coats of the model legume Medicago truncatula, reaching maximal levels at around 20 d after pollination. Genes encoding the single Medicago anthocyanidin synthase (ANS; EC 1.14.11.19) and leucoanthocyanidin reductase (LAR...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.107326
更新日期:2007-11-01 00:00:00
abstract::An experimental chemical N-[2-(2-oxo-1-imidazolidinyl)ethyl]-N'-phenylurea (EDU), is an effective protectant against acute and chronic foliar injury due to ozone (0(3)) when sprayed on intact leaves or supplied to the plants through soil application. An 0(3)-sensitive snap bean cultivar (Phaseolus vulgaris L. ;Bush Bl...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.6.1444
更新日期:1982-06-01 00:00:00
abstract::Topped plants of tomato (Lycopersicon esculentum), cucumber (Cucumis sativus), corn (Zea mays), carrot (Daucus carota), and peanut (Arachis hypogaea) were treated with 0.5 to 50 micromolar Ni (containing (63)Ni) in nutrient solutions. Xylem exudate was collected for 10 hours or, in the case of corn, for 20 hours at 5-...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.48.3.273
更新日期:1971-09-01 00:00:00
abstract::Virus-induced gene silencing (VIGS) is used extensively for gene function studies in plants. VIGS is inexpensive and rapid compared with silencing conducted through stable transformation, but many virus-silencing vectors, especially in grasses, induce only transient silencing phenotypes. A major reason for transient p...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.00905
更新日期:2018-01-01 00:00:00
abstract::Seedlings of maize (Zea mays L. cv Pioneer 3906), hydroponically grown in the dark, were exposed to NaCl either gradually (salt acclimation) or in one step (salt shock). In the salt-acclimation treatment, root extension was indistinguishable from that of unsalinized controls for at least 6 d at concentrations up to 10...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.3.881
更新日期:1997-03-01 00:00:00
abstract::Seven-day-old dark-grown bean leaves were greened under continuous light. The amount of chlorophyll, the ratio of chlorophyll a to chlorophyll b, the O(2) evolving capacity and the primary photochemical activities of Photosystem I and Photosystem II were measured on the leaves after various times of greening. The prim...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.4.526
更新日期:1976-10-01 00:00:00
abstract::Branched-chain amino acids (BCAAs) are synthesized in plants from branched-chain keto acids, but their metabolism is not completely understood. The interface of BCAA metabolism lies with branched-chain aminotransferases (BCAT) that catalyze both the last anabolic step and the first catabolic step. In this study, six B...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.110.154922
更新日期:2010-07-01 00:00:00
abstract::Indoleacetic acid-induced ethylene production and growth in excised segments of etiolated pea shoots (Pisum sativum L. var. Alaska) parallels the free indoleacetic acid level in the tissue which in turn depends upon the rate of indoleacetic acid conjugation and decarboxylation. Both ethylene synthesis and growth requi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.47.4.504
更新日期:1971-04-01 00:00:00
abstract::One of the most abundant proteins in maize (Zea mays L.) embryos is the molecular weight 63,000 globulin encoded by the Glb1 gene. To obtain DNA clones corresponding to Glb1, a cDNA library corresponding to RNA from developing maize embryos was constructed in a lambda expression vector and screened with antibodies spe...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.91.2.636
更新日期:1989-10-01 00:00:00
abstract::The Arabidopsis genome encodes four Dicer-like (DCL) proteins, two of which contain putative nuclear localization signals. This suggests one or more nuclear pathways for processing double-stranded (ds) RNA in plants. To study the subcellular location of processing of nuclear-encoded dsRNA involved in transcriptional s...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.103.021980
更新日期:2003-07-01 00:00:00