Passive nitrate transport by root plasma membrane vesicles exhibits an acidic optimal pH like the H(+)-ATPase.

Abstract:

:The net initial passive flux (J(Ni)) in reconstituted plasma membrane (PM) vesicles from maize (Zea mays) root cells was measured as recently described (P. Pouliquin, J.-P. Grouzis, R. Gibrat ¿1999 Biophys J 76: 360-373). J(Ni) in control liposomes responded to membrane potential or to NO(3)(-) as expected from the Goldman-Hodgkin-Katz diffusion theory. J(Ni) in reconstituted PM vesicles exhibited an additional component (J(Nif)), which was saturable (K(m) for NO(3)(-) approximately 3 mM, with J(Nifmax) corresponding to 60 x 10(-9) mol m(-2) s(-1) at the native PM level) and selective (NO(3)(-) = ClO(3)(-) > Br(-) > Cl(-) = NO(2)(-); relative fluxes at 5 mM: 1:0.34:0.19). J(Nif) was totally inhibited by La(3+) and the arginine reagent phenylglyoxal. J(Nif) was voltage dependent, with an optimum voltage at 105 mV at pH 6.5. The activation energy of J(Nif) was high (129 kJ mol(-1)), close to that of the H(+)-ATPase (155 kJ mol(-1)), and J(Nif) displayed the same acidic optimal pH (pH 6.5) as that of the H(+) pump. This is the first example, to our knowledge, of a secondary transport at the plant PM with such a feature. Several properties of the NO(3)(-) uniport seem poorly compatible with that reported for plant anion channels and to be attributable instead to a classical carrier. The physiological relevance of these findings is suggested.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Pouliquin P,Boyer JC,Grouzis JP,Gibrat R

doi

10.1104/pp.122.1.265

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

265-74

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

122

pub_type

杂志文章
  • Modulation of PSI and PSII Organization During Loss and Repair of Photosynthetic Activity in a Temperature Sensitive Mutant of Chlorella pyrenoidosa.

    abstract::Photosynthetic activity and organization of chlorophyll(Chl)-protein complexes in a temperature sensitive mutant of Chlorella pyrenoidosa have been investigated. The mutant is practically indistinguishable from wild type cells when grown at 25 C. However, mutant cells grown at 33 C do not synthesize Chl and lose their...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.6.1264

    authors: Lavintman N,Galling G,Ohad I

    更新日期:1981-12-01 00:00:00

  • Identification of methyl jasmonate and salicylic acid response elements from the nopaline synthase (nos) promoter.

    abstract::Transgenic tobacco plants carrying a fusion between the nopaline synthase (nos) promoter and chloramphenicol acetyltransferase (CAT) reporter gene (cat) were studied for their inducibility by salicylic acid (SA) or methyl jasmonate (MJ) treatments. Either chemical significantly increased CAT activity to a level much h...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.103.1.97

    authors: Kim SR,Kim Y,An G

    更新日期:1993-09-01 00:00:00

  • A Plant Virus-Based Vector System for Gene Function Studies in Pepper.

    abstract::While pepper (Capsicum annuum) is a highly recalcitrant species for genetic transformation studies, plant virus-based vectors can provide alternative and powerful tools for transient regulation and functional analysis of genes of interest in pepper. In this study, we established an effective virus-based vector system ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00836

    authors: Choi B,Kwon SJ,Kim MH,Choe S,Kwak HR,Kim MK,Jung C,Seo JK

    更新日期:2019-11-01 00:00:00

  • Suppression of both ELIP1 and ELIP2 in Arabidopsis does not affect tolerance to photoinhibition and photooxidative stress.

    abstract::ELIPs (early light-induced proteins) are thylakoid proteins transiently induced during greening of etiolated seedlings and during exposure to high light stress conditions. This expression pattern suggests that these proteins may be involved in the protection of the photosynthetic apparatus against photooxidative damag...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.083055

    authors: Rossini S,Casazza AP,Engelmann EC,Havaux M,Jennings RC,Soave C

    更新日期:2006-08-01 00:00:00

  • Preparation of Membrane Vesicles Enriched in ATP-Dependent Proton Transport from Suspension Cultures of Tomato Cells.

    abstract::Membranes enriched in ATP-dependent proton transport were prepared from suspension cultures of tomato cells (Lycopersicon esculentum Mill cv VF36). Suspension cultures were a source of large quantities of membranes from rapidly growing, undifferentiated cells. Proton transport activity was assayed as quench of acridin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.1.69

    authors: Dupont FM,Zabala Mde G

    更新日期:1985-01-01 00:00:00

  • Inhibition of leaf process by p-fluorophenylalanine during induction of flowering in the cocklebur.

    abstract::The amino acid antimetabolite, DL-p-fluorophenylalanine (FPA), inhibited induction of flowering in the short-day cocklebur plant, Xanthium pensylvanicum Wall., primarily by interfering with processes occurring during the inductive dark period. At the concentrations used the inhibitor had little effect on subsequent ve...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.41.7.1185

    authors: Miller J,Ross C

    更新日期:1966-09-01 00:00:00

  • Quantification of free plus conjugated indoleacetic acid in arabidopsis requires correction for the nonenzymatic conversion of indolic nitriles.

    abstract::The genetic advantages to the use of Arabidopsis thaliana mutants for the study of auxin metabolism previously have been partially offset by the complexity of indolic metabolism in this plant and by the lack of proper methods. To address some of these problems, we developed isotopic labeling methods to determine amoun...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.3.781

    authors: Llić N,Normanly J,Cohen JD

    更新日期:1996-07-01 00:00:00

  • A bioinformatics approach to the identification, classification, and analysis of hydroxyproline-rich glycoproteins.

    abstract::Hydroxyproline-rich glycoproteins (HRGPs) are a superfamily of plant cell wall proteins that function in diverse aspects of plant growth and development. This superfamily consists of three members: hyperglycosylated arabinogalactan proteins (AGPs), moderately glycosylated extensins (EXTs), and lightly glycosylated pro...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.156554

    authors: Showalter AM,Keppler B,Lichtenberg J,Gu D,Welch LR

    更新日期:2010-06-01 00:00:00

  • The velocities of ion transport into and through the xylem of roots: findings with a two-point application pulse-chase technique.

    abstract::Excised corn roots, Zea mays, had radioactively labeled solution applied at two points along their length, for 1 minute, and were then kept in dilute, unlabeled nutrient solution. During this "chase" period, exudate was collected at 1-minute intervals, and its content of radioions was determined. Two pulses of label a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.4.346

    authors: Epstein E,Norlyn JD

    更新日期:1973-10-01 00:00:00

  • Endogenous NO(3) in the Root as a Source of Substrate for Reduction in the Light.

    abstract::An experiment was conducted to investigate the reduction of endogenous NO(3) (-), which had been taken up by plants in darkness, during the course of the subsequent light period. Vegetative, nonnodulated soybean plants (Glycine max [L]. Merrill, ;Ransom') were exposed to 1.0 millimolar (15)NO(3) (-) for 12 hours in da...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.4.1421

    authors: Rufty TW,Volk RJ,Mackown CT

    更新日期:1987-08-01 00:00:00

  • Arabidopsis seed development and germination is associated with temporally distinct metabolic switches.

    abstract::While the metabolic networks in developing seeds during the period of reserve accumulation have been extensively characterized, much less is known about those present during seed desiccation and subsequent germination. Here we utilized metabolite profiling, in conjunction with selective mRNA and physiological profilin...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.086694

    authors: Fait A,Angelovici R,Less H,Ohad I,Urbanczyk-Wochniak E,Fernie AR,Galili G

    更新日期:2006-11-01 00:00:00

  • Metabolism of D-glycero-D-manno-heptitol, volemitol, in polyanthus. Discovery Of a novel ketose reductase1

    abstract::Volemitol (D-glycero-D-manno-heptitol, alpha-sedoheptitol) is an unusual seven-carbon sugar alcohol that fulfills several important physiological functions in certain species of the genus Primula. Using the horticultural hybrid polyanthus (Primula x polyantha) as our model plant, we found that volemitol is the major n...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.119.1.191

    authors: Hafliger B,Kindhauser E,Keller F

    更新日期:1999-01-01 00:00:00

  • Soybean Seed Water Relations during in Situ and in Vitro Growth and Maturation.

    abstract::Water, osmotic, and pressure potentials of soybean (Glycine max [L.] Merrill) embryos and related maternal tissues were measured during periods of seed growth and maturation to test the involvement of embryo water relations in seed maturation. Seeds were matured in situ or in an in vitro liquid culture medium in detac...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.2.610

    authors: Saab IN,Obendorf RL

    更新日期:1989-02-01 00:00:00

  • Mechanism of Stimulation and Inhibition of Tonoplast H-ATPase of Beta vulgaris by Chloride and Nitrate.

    abstract::The H(+)-ATPase of tonoplast vesicles isolated from red beet (Beta vulgaris L.) storage tissue was studied with respect to the kinetic effects of Cl(-) and NO(3) (-). N-Ethylmaleimide (NEM) was employed as a probe to investigate substrate binding and gross conformational changes of the enzyme. Chloride decreased the K...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.81.1.120

    authors: Griffith CJ,Rea PA,Blumwald E,Poole RJ

    更新日期:1986-05-01 00:00:00

  • Purification and Developmental Analysis of an Extracellular Proteinase from Young Leaves of Soybean.

    abstract::A proteinase present in intercellular wash fluids from leaves of Glycine max has been purified 600-fold to electrophoretic homogeneity. The native protein is monomeric with a molecular mass of 60 kD, as estimated by denaturing gel electrophoresis, and has an isoelectric point of 7.7. The enzyme has a pH optimum of 9.5...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.3.969

    authors: Huangpu J,Graham JS

    更新日期:1995-07-01 00:00:00

  • Lignification in sugarcane: biochemical characterization, gene discovery, and expression analysis in two genotypes contrasting for lignin content.

    abstract::Sugarcane (Saccharum spp.) is currently one of the most efficient crops in the production of first-generation biofuels. However, the bagasse represents an additional abundant lignocellulosic resource that has the potential to increase the ethanol production per plant. To achieve a more efficient conversion of bagasse ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.225250

    authors: Bottcher A,Cesarino I,Santos AB,Vicentini R,Mayer JL,Vanholme R,Morreel K,Goeminne G,Moura JC,Nobile PM,Carmello-Guerreiro SM,Anjos IA,Creste S,Boerjan W,Landell MG,Mazzafera P

    更新日期:2013-12-01 00:00:00

  • Identification of Putative Substrates of SEC2, a Chloroplast Inner Envelope Translocase.

    abstract::Most chloroplast proteins are synthesized in the cytosol and imported into chloroplasts. Many imported proteins are further targeted to the thylakoid membrane and lumen by the SEC1, TAT, or SRP/ALB3 translocases. Others are targeted to the inner chloroplast envelope membrane by undescribed translocases. Recently, a se...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00012

    authors: Li Y,Martin JR,Aldama GA,Fernandez DE,Cline K

    更新日期:2017-04-01 00:00:00

  • Inhibition of plant glutamine synthetases by substituted phosphinothricins.

    abstract::Glutamine synthetase (GS) utilizes various substituted glutamic acids as substrates. We have used this information to design herbicidal alpha- and gamma-substituted analogs of phosphinothricin (l-2-amino-4-(hydroxymethylphosphinyl)butanoic acid, PPT), a naturally occurring GS inhibitor and a potent herbicide. The subs...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.4.1057

    authors: Logusch EW,Walker DM,McDonald JF,Franz JE

    更新日期:1991-04-01 00:00:00

  • The Ethylene-insensitive sickle mutant of Medicago truncatula shows altered auxin transport regulation during nodulation.

    abstract::We studied the ethylene-insensitive, hypernodulating mutant, sickle (skl), to investigate the interaction of ethylene with auxin transport during root nodulation in Medicago truncatula. Grafting experiments demonstrated that hypernodulation in skl is root controlled. Long distance transport of auxin from shoot to root...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.080093

    authors: Prayitno J,Rolfe BG,Mathesius U

    更新日期:2006-09-01 00:00:00

  • Analysis of cell division and elongation underlying the developmental acceleration of root growth in Arabidopsis thaliana.

    abstract::To investigate the relation between cell division and expansion in the regulation of organ growth rate, we used Arabidopsis thaliana primary roots grown vertically at 20 degreesC with an elongation rate that increased steadily during the first 14 d after germination. We measured spatial profiles of longitudinal veloci...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.116.4.1515

    authors: Beemster GT,Baskin TI

    更新日期:1998-04-01 00:00:00

  • The Patterned Structure of Galactoglucomannan Suggests It May Bind to Cellulose in Seed Mucilage.

    abstract::The interaction between mannan polysaccharides and cellulose microfibrils contributes to cell wall properties in some vascular plants, but the molecular arrangement of mannan in the cell wall and the nature of the molecular bonding between mannan and cellulose remain unknown. Previous studies have shown that mannan is...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.00709

    authors: Yu L,Lyczakowski JJ,Pereira CS,Kotake T,Yu X,Li A,Mogelsvang S,Skaf MS,Dupree P

    更新日期:2018-11-01 00:00:00

  • Intracellular beta-carbonic anhydrase of the unicellular green alga Coccomyxa. Cloning of the cdna and characterization of the functional enzyme overexpressed in Escherichia coli.

    abstract::Carbonic anhydrase (CA) (EC 4.2.1.1) enzymes catalyze the reversible hydration of CO2, a reaction that is important in many physiological processes. We have cloned and sequenced a full-length cDNA encoding an intracellular beta-CA from the unicellular green alga Coccomyxa. Nucleotide sequence data show that the isolat...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.4.1341

    authors: Hiltonen T,Björkbacka H,Forsman C,Clarke AK,Samuelsson G

    更新日期:1998-08-01 00:00:00

  • Vacuolar Transporters - Companions on a Longtime Journey.

    abstract::Biochemical and electrophysiological studies on plant vacuolar transporters became feasible in the late 1970s and early 1980s, when methods to isolate large quantities of intact vacuoles and purified vacuolar membrane vesicles were established. However, with the exception of the H+-ATPase and H+-PPase, which could be ...

    journal_title:Plant physiology

    pub_type: 杂志文章,评审

    doi:10.1104/pp.17.01481

    authors: Martinoia E

    更新日期:2018-02-01 00:00:00

  • Maintenance of air in intercellular spaces of plants.

    abstract::Although air-filled intercellular spaces are necessary and ubiquitous in higher plants, little attention has been paid to the possible mechanisms by which these spaces are kept from being flooded. The most likely mechanism is that the living plant cell may maintain a hydrophobic monolayer on the surfaces of adjacent i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.4.989

    authors: Woolley JT

    更新日期:1983-08-01 00:00:00

  • The Multisite PeachRefPop Collection: A True Cultural Heritage and International Scientific Tool for Fruit Trees.

    abstract::Plants have evolved a range of adaptive mechanisms that adjust their development and physiology to variable external conditions, particularly in perennial species subjected to long-term interplay with the environment. Exploiting the allelic diversity within available germplasm and leveraging the knowledge of the mecha...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01412

    authors: Cirilli M,Micali S,Aranzana MJ,Arús P,Babini A,Barreneche T,Bink M,Cantin CM,Ciacciulli A,Cos-Terrer JE,Drogoudi P,Eduardo I,Foschi S,Giovannini D,Guerra W,Liverani A,Pacheco I,Pascal T,Quilot-Turion B,Verde I,Ros

    更新日期:2020-10-01 00:00:00

  • Molecular structure of three mutations at the maize sugary1 locus and their allele-specific phenotypic effects.

    abstract::Starch production in all plants examined is altered by mutations of isoamylase-type starch-debranching enzymes (DBE), although how these proteins affect glucan polymer assembly is not understood. Various allelic mutations in the maize (Zea mays) gene sugary1 (su1), which codes for an isoamylase-type DBE, condition dis...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.125.3.1406

    authors: Dinges JR,Colleoni C,Myers AM,James MG

    更新日期:2001-03-01 00:00:00

  • The Arabidopsis aleurone layer responds to nitric oxide, gibberellin, and abscisic acid and is sufficient and necessary for seed dormancy.

    abstract::Seed dormancy is a common phase of the plant life cycle, and several parts of the seed can contribute to dormancy. Whole seeds, seeds lacking the testa, embryos, and isolated aleurone layers of Arabidopsis (Arabidopsis thaliana) were used in experiments designed to identify components of the Arabidopsis seed that cont...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.093435

    authors: Bethke PC,Libourel IG,Aoyama N,Chung YY,Still DW,Jones RL

    更新日期:2007-03-01 00:00:00

  • Gain-of-Function Mutants of the Cytokinin Receptors AHK2 and AHK3 Regulate Plant Organ Size, Flowering Time and Plant Longevity.

    abstract::The phytohormone cytokinin is a regulator of numerous processes in plants. In Arabidopsis (Arabidopsis thaliana), the cytokinin signal is perceived by three membrane-located receptors named ARABIDOPSIS HISTIDINE KINASE2 (AHK2), AHK3, and AHK4/CRE1. How the signal is transmitted across the membrane is an entirely unkno...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01903

    authors: Bartrina I,Jensen H,Novák O,Strnad M,Werner T,Schmülling T

    更新日期:2017-03-01 00:00:00

  • Functional and expressional analyses of PmDAM genes associated with endodormancy in Japanese apricot.

    abstract::Bud endodormancy in woody plants plays an important role in their perennial growth cycles. We previously identified a MADS box gene, DORMANCY-ASSOCIATED MADS box6 (PmDAM6), expressed in the endodormant lateral buds of Japanese apricot (Prunus mume), as a candidate for the dormancy-controlling gene. In this study, we d...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.111.181982

    authors: Sasaki R,Yamane H,Ooka T,Jotatsu H,Kitamura Y,Akagi T,Tao R

    更新日期:2011-09-01 00:00:00

  • Effect of phosphoenolpyruvate and oxaloacetate on ca uptake by isolated mung bean mitochondria.

    abstract::Phosphoenolpyruvate partially inhibits the accumulation of Ca(2+) in isolated mung bean (Phaseolus aureus Roxb.) mitochondria. Succinate-supported Ca(2+) uptake is twice as sensitive to phosphoenolpyruvate inhibition as is NADH- or malate/pyruvate-supported Ca(2+) uptake. Pyruvate, atractylate, and ATP, but not ITP, r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.2.126

    authors: Graesser RJ

    更新日期:1977-02-01 00:00:00