Thioredoxin-Mediated ROS Homeostasis Explains Natural Variation in Plant Regeneration.

Abstract:

:Plant regeneration is fundamental to basic research and agricultural applications. The regeneration capacity of plants varies largely in different genotypes, but the reason for this variation remains elusive. Here, we identified a novel thioredoxin DCC1 in determining the capacity of shoot regeneration among Arabidopsis (Arabidopsis thaliana) natural variation. Loss of function of DCC1 resulted in inhibited shoot regeneration. DCC1 was expressed mainly in the inner tissues of the callus and encoded a functional thioredoxin that was localized in the mitochondria. DCC1 protein interacted directly with CARBONIC ANHYDRASE2 (CA2), which is an essential subunit of the respiratory chain NADH dehydrogenase complex (Complex I). DCC1 regulated Complex I activity via redox modification of CA2 protein. Mutation of DCC1 or CA2 led to reduced Complex I activity and triggered mitochondrial reactive oxygen species (ROS) production. The increased ROS level regulated shoot regeneration by repressing expression of the genes involved in multiple pathways. Furthermore, linkage disequilibrium analysis indicated that DCC1 was a major determinant of the natural variation in shoot regeneration among Arabidopsis ecotypes. Thus, our study uncovers a novel regulatory mechanism by which thioredoxin-dependent redox modification regulates de novo shoot initiation via the modulation of ROS homeostasis and provides new insights into improving the capacity of plant regeneration.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Zhang H,Zhang TT,Liu H,Shi Y,Wang M,Bie XM,Li XG,Zhang XS

doi

10.1104/pp.17.00633

subject

Has Abstract

pub_date

2018-03-01 00:00:00

pages

2231-2250

issue

3

eissn

0032-0889

issn

1532-2548

pii

pp.17.00633

journal_volume

176

pub_type

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