Stress Responses in Alfalfa (XXI. Activation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl Coenzyme A 3-O-Methyltransferase Genes Does Not Contribute to Changes in Metabolite Accumulation in Elicitor-Treated Cell-Suspension Cultures).

Abstract:

:Transcription of genes encoding L-phenylalanine ammonia-lyase (PAL), the first enzyme of the phenylpropanoid pathway, and caffeic acid 3-O-methyltransferase (COMT) and caffeoyl CoA 3-O-methyltransferase (CCOMT), enzymes involved in the synthesis of lignin and wall-esterified phenolic compounds, was strongly activated in elicitor-treated cell-suspension cultures of alfalfa (Medicago sativa L.). However, consequent changes in the extractable activities of COMT and CCOMT were small to nonexistent compared with a 15- to 16-fold increase in PAL activity. Only low levels of COMT and CCOMT transcripts were reflected in the total and polysomal RNA fractions compared with PAL transcripts. Elicited cell cultures did not accumulate lignin or the products of COMT and CCOMT in the soluble and wall-esterified phenolic fractions. In one alfalfa cell line in which elicitation resulted in very high PAL activity and increased deposition of methoxyl groups in the insoluble wall fraction, there was still no change in COMT and CCOMT activities. Overall, these results indicate that the initial gene transcription events in elicited cells may be less selective than the subsequent metabolic changes, highlighting the importance of posttranscriptional events in the control of phenylpropanoid biosynthesis.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Ni W,Sewalt V,Korth KL,Blount JW,Ballance GM,Dixon RA

doi

10.1104/pp.112.2.717

subject

Has Abstract

pub_date

1996-10-01 00:00:00

pages

717-726

issue

2

eissn

0032-0889

issn

1532-2548

pii

112/2/717

journal_volume

112

pub_type

杂志文章
  • Arabidopsis map-based cloning in the post-genome era.

    abstract::Map-based cloning is an iterative approach that identifies the underlying genetic cause of a mutant phenotype. The major strength of this approach is the ability to tap into a nearly unlimited resource of natural and induced genetic variation without prior assumptions or knowledge of specific genes. One begins with an...

    journal_title:Plant physiology

    pub_type: 杂志文章,评审

    doi:10.1104/pp.003533

    authors: Jander G,Norris SR,Rounsley SD,Bush DF,Levin IM,Last RL

    更新日期:2002-06-01 00:00:00

  • Short-term leaf elongation kinetics of maize in response to salinity are independent of the root.

    abstract::The essentiality of roots to the short-term responses of leaf elongation to salinity was tested by removing the roots of maize (Zea mays L.) from the shoots and comparing the initial short-term response of leaf elongation to that with intact plants. Eightday-old seedlings growing in solution culture were treated with ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.3.965

    authors: Cramer GR,Bowman DC

    更新日期:1991-03-01 00:00:00

  • Endogenous gibberellin and abscisic Acid content as related to senescence of detached lettuce leaves.

    abstract::Levels of gibberillins (GAs) and of abscisic acid (ABA) in attached leaves of romaine lettuce (Lactuca sativa L.) declined as the leaf became older. The time course of changes in hormone levels, determined in detached lettuce leaves kept in darkness, revealed that a sharp decline in GAs accompanied by a moderate rise ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.62.2.224

    authors: Aharoni N

    更新日期:1978-08-01 00:00:00

  • Changes to the Stoichiometry of Glycine Decarboxylase Subunits during Wheat (Triticum aestivum L.) and Pea (Pisum sativum L.) Leaf Development.

    abstract::Changes in the levels of the four subunits of the mitochondrial enzyme glycine decarboxylase (EC 2.1.2.10) have been investigated during development in the 8 day old primary leaf of wheat (Triticum aestivum L.). Proteins were extracted from wheat leaf sections between the basal meristem and 8.5 centimeters. The indivi...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.3.952

    authors: Rogers WJ,Jordan BR,Rawsthorne S,Tobin AK

    更新日期:1991-07-01 00:00:00

  • Inhibition of the formation of photosynthetic enzymes by inhibitors of photosynthesis.

    abstract::It has been shown previously that an increase in ribulose diphosphate carboxylase activity occurs upon brief illumination of leaves of dark-grown Zea mays plants; an increase in ribose 5-phosphate isomerase occurs after prolonged illumination. Both of these responses to illumination are inhibited by chloramphenicol.Th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.2.188

    authors: McMahon D,Bogorad L

    更新日期:1968-02-01 00:00:00

  • Magnitude and Kinetics of Stem Elongation Induced by Exogenous Indole-3-Acetic Acid in Intact Light-Grown Pea Seedlings.

    abstract::Exogenously applied indole-3-acetic acid (IAA) strongly promoted stem elongation over the long term in intact light-grown seedlings of both dwarf (cv Progress No. 9) and tall (cv Alaska) peas (Pisum sativum L.), with the relative promotion being far greater in dwarf plants. In dwarf seedlings, solutions of IAA (betwee...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.102.3.717

    authors: Yang T,Law DM,Davies PJ

    更新日期:1993-07-01 00:00:00

  • Chlorophyll biosynthesis. Expression of a second chl I gene of magnesium chelatase in Arabidopsis supports only limited chlorophyll synthesis.

    abstract::Magnesium (Mg) chelatase is a heterotrimeric enzyme complex that catalyzes a key regulatory and enzymatic reaction in chlorophyll biosynthesis, the insertion of Mg(2+) into protoporphyrin IX. Studies of the enzyme complex reconstituted in vitro have shown that all three of its subunits, CHL I, CHL D, and CHL H, are re...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010625

    authors: Rissler HM,Collakova E,DellaPenna D,Whelan J,Pogson BJ

    更新日期:2002-02-01 00:00:00

  • Overexpression of an isoprenyl diphosphate synthase in spruce leads to unexpected terpene diversion products that function in plant defense.

    abstract::Spruce (Picea spp.) and other conifers employ terpenoid-based oleoresin as part of their defense against herbivores and pathogens. The short-chain isoprenyl diphosphate synthases (IDS) are situated at critical branch points in terpene biosynthesis, producing the precursors of the different terpenoid classes. To determ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.228940

    authors: Nagel R,Berasategui A,Paetz C,Gershenzon J,Schmidt A

    更新日期:2014-02-01 00:00:00

  • Molecular Characterization of Arabidopsis GAL4/UAS Enhancer Trap Lines Identifies Novel Cell-Type-Specific Promoters.

    abstract::Cell-type-specific gene expression is essential to distinguish between the numerous cell types of multicellular organism. Therefore, cell-type-specific gene expression is tightly regulated and for most genes RNA transcription is the central point of control. Thus, transcriptional reporters are broadly used markers for...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.00213

    authors: Radoeva T,Ten Hove CA,Saiga S,Weijers D

    更新日期:2016-06-01 00:00:00

  • Expression of foreign genes in transgenic yellow-poplar plants.

    abstract::Cells of yellow-poplar (Liriodendron tulipifera L.) were transformed by direct gene transfer and regenerated into plants by somatic embryogenesis. Plasmid DNA bearing marker genes encoding beta-glucuronidase (GUS) and neomycin phosphotransferase (NPT II) were introduced by microprojectile bombardment into single cells...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.1.114

    authors: Wilde HD,Meagher RB,Merkle SA

    更新日期:1992-01-01 00:00:00

  • TAP46 plays a positive role in the ABSCISIC ACID INSENSITIVE5-regulated gene expression in Arabidopsis.

    abstract::TAP46 is a protein phosphatase2A (PP2A)-associated protein that regulates PP2A activity in Arabidopsis (Arabidopsis thaliana). To study how PP2A is involved in abscisic acid (ABA) signaling in plants, we studied the function of TAP46 in ABA-regulated seed maturation and seedling development. Expression of TAP46 coinci...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.233684

    authors: Hu R,Zhu Y,Shen G,Zhang H

    更新日期:2014-02-01 00:00:00

  • Soybean root suberin: anatomical distribution, chemical composition, and relationship to partial resistance to Phytophthora sojae.

    abstract::Soybean (Glycine max L. Merr.) is a versatile and important agronomic crop grown worldwide. Each year millions of dollars of potential yield revenues are lost due to a root rot disease caused by the oomycete Phytophthora sojae (Kaufmann & Gerdemann). Since the root is the primary site of infection by this organism, we...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.091090

    authors: Thomas R,Fang X,Ranathunge K,Anderson TR,Peterson CA,Bernards MA

    更新日期:2007-05-01 00:00:00

  • Proton Transport Activity of the Purified Vacuolar H-ATPase from Oats : Direct Stimulation by Cl.

    abstract::To determine whether the detergent-solubilized and purified vacuolar H(+)-ATPase from plants was active in H(+) transport, we reconstituted the purified vacuolar ATPase from oat roots (Avena sativa var Lang). Triton-solubilized ATPase activity was purified by gel filtration and ion exchange chromatography. Incorporati...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.3.925

    authors: Ward JM,Sze H

    更新日期:1992-07-01 00:00:00

  • Freeze-thaw stress: effects of temperature on hydraulic conductivity and ultrasonic activity in ten woody angiosperms.

    abstract::Freeze-thaw events can affect plant hydraulics by inducing embolism. This study analyzed the effect of temperature during the freezing process on hydraulic conductivity and ultrasonic emissions (UE). Stems of 10 angiosperms were dehydrated to a water potential at 12% percentage loss of hydraulic conductivity (PLC) and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.228403

    authors: Charrier G,Charra-Vaskou K,Kasuga J,Cochard H,Mayr S,Améglio T

    更新日期:2014-02-01 00:00:00

  • Arabidopsis RING E3 ligase XBAT32 regulates lateral root production through its role in ethylene biosynthesis.

    abstract::XBAT32, a member of the RING domain-containing ankyrin repeat subfamily of E3 ligases, was previously identified as a positive regulator of lateral root development. Arabidopsis (Arabidopsis thaliana) plants harboring a mutation in XBAT32 produce fewer lateral roots that wild-type plants. We found that xbat32 mutants ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.156976

    authors: Prasad ME,Schofield A,Lyzenga W,Liu H,Stone SL

    更新日期:2010-08-01 00:00:00

  • Two young MicroRNAs originating from target duplication mediate nitrogen starvation adaptation via regulation of glucosinolate synthesis in Arabidopsis thaliana.

    abstract::Nitrogen is an essential macronutrient required for plant growth and development. A number of genes respond to nitrogen starvation conditions. However, the functions of most of these nitrogen starvation-responsive genes are unclear. Our recent survey suggested that many microRNAs (miRNAs) are responsive to nitrogen st...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.228635

    authors: He H,Liang G,Li Y,Wang F,Yu D

    更新日期:2014-02-01 00:00:00

  • A grapevine gene encoding a guard cell K(+) channel displays developmental regulation in the grapevine berry.

    abstract::SIRK is a K(+) channel identified in grapevine (Vitis vinifera), belonging to the so-called Shaker family. The highest sequence similarities it shares with the members of this family are found with channels of the KAT type, although SIRK displays a small ankyrin domain. This atypical feature provides a key to understa...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.010529

    authors: Pratelli R,Lacombe B,Torregrosa L,Gaymard F,Romieu C,Thibaud JB,Sentenac H

    更新日期:2002-02-01 00:00:00

  • Initial events during the evolution of C4 photosynthesis in C3 species of Flaveria.

    abstract::The evolution of C4 photosynthesis in many taxa involves the establishment of a two-celled photorespiratory CO2 pump, termed C2 photosynthesis. How C3 species evolved C2 metabolism is critical to understanding the initial phases of C4 plant evolution. To evaluate early events in C4 evolution, we compared leaf anatomy,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.221119

    authors: Sage TL,Busch FA,Johnson DC,Friesen PC,Stinson CR,Stata M,Sultmanis S,Rahman BA,Rawsthorne S,Sage RF

    更新日期:2013-11-01 00:00:00

  • Specific gene silencing by artificial MicroRNAs in Physcomitrella patens: an alternative to targeted gene knockouts.

    abstract::MicroRNAs (miRNAs) are approximately 21-nucleotide-long RNAs processed from nuclear-encoded transcripts, which include a characteristic hairpin-like structure. MiRNAs control the expression of target transcripts by binding to reverse complementary sequences directing cleavage or translational inhibition of the target ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.128025

    authors: Khraiwesh B,Ossowski S,Weigel D,Reski R,Frank W

    更新日期:2008-10-01 00:00:00

  • Soft X-Ray Imaging of Cellular Carbon and Nitrogen Distributions in Heterocystous Cyanobacteria.

    abstract::Soft x-ray microscopy (SXM) is a minimally invasive technique for single-cell high-resolution imaging as well as the visualization of intracellular distributions of light elements such as carbon, nitrogen, and oxygen. We used SXM to observe photosynthesis and nitrogen fixation in the filamentous cyanobacterium Anabaen...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.01767

    authors: Teramoto T,Azai C,Terauchi K,Yoshimura M,Ohta T

    更新日期:2018-05-01 00:00:00

  • The internal propeptide of the ricin precursor carries a sequence-specific determinant for vacuolar sorting.

    abstract::Ricin is a heterodimeric toxin that accumulates in the storage vacuoles of castor bean (Ricinus communis) endosperm. Proricin is synthesized as a single polypeptide precursor comprising the catalytic A chain and the Gal-binding B chain joined by a 12-amino acid linker propeptide. Upon arrival in the vacuole, the linke...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.126.1.167

    authors: Frigerio L,Jolliffe NA,Di Cola A,Felipe DH,Paris N,Neuhaus JM,Lord JM,Ceriotti A,Roberts LM

    更新日期:2001-05-01 00:00:00

  • Modification of logarithmic growth rates of tobacco callus tissue by gibberellic Acid.

    abstract::Logarithmic growth rates (either fresh or dry weight basis) of tobacco callus tissues grown on 10(-4) to 10(-1) mum cytokinin are increased if gibberellic acid (10(-3)-2 mum) is incorporated into the medium. At higher (1-10 mum) cytokinin concentrations gibberellic acid has little effect on growth rate but extends the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.46.1.113

    authors: Helgeson JP,Upper CD

    更新日期:1970-07-01 00:00:00

  • Mesoporous silica nanoparticle-mediated intracellular cre protein delivery for maize genome editing via loxP site excision.

    abstract::The delivery of proteins instead of DNA into plant cells allows for a transient presence of the protein or enzyme that can be useful for biochemical analysis or genome modifications. This may be of particular interest for genome editing, because it can avoid DNA (transgene) integration into the genome and generate pre...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.233650

    authors: Martin-Ortigosa S,Peterson DJ,Valenstein JS,Lin VS,Trewyn BG,Lyznik LA,Wang K

    更新日期:2014-02-01 00:00:00

  • Sucrose phosphate synthase, a key enzyme for sucrose biosynthesis in plants: protein purification from corn leaves and immunological detection.

    abstract::We have purified the protein for the enzyme sucrose phosphate synthase (SPS) from corn (Zea mays) leaves. Partially purified SPS protein was used to generate specific monoclonal antibodies. The following immunoaffinity chromatography allowed the isolation of pure SPS protein. The apparent molecular mass of the SPS pol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.2.473

    authors: Bruneau JM,Worrell AC,Cambou B,Lando D,Voelker TA

    更新日期:1991-06-01 00:00:00

  • Predicting Dynamic Metabolic Demands in the Photosynthetic Eukaryote Chlorella vulgaris.

    abstract::Phototrophic organisms exhibit a highly dynamic proteome, adapting their biomass composition in response to diurnal light/dark cycles and nutrient availability. Here, we used experimentally determined biomass compositions over the course of growth to determine and constrain the biomass objective function (BOF) in a ge...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00605

    authors: Zuñiga C,Levering J,Antoniewicz MR,Guarnieri MT,Betenbaugh MJ,Zengler K

    更新日期:2018-01-01 00:00:00

  • Photosynthetic Genes of Petunia (Mitchell) Are Differentially Expressed during the Diurnal Cycle.

    abstract::The petunia (Petunia [Mitchell]) chloroplast proteins, the chlorophyll a/b-binding (Cab) proteins, and the small subunit of ribulose bisphosphate carboxylase (RbcS) are encoded by nuclear genes that are expressed in a light-dependent manner. The steady-state concentrations of five cab mRNAs vary with a dramatic circad...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.89.3.776

    authors: Stayton MM,Brosio P,Dunsmuir P

    更新日期:1989-03-01 00:00:00

  • Trehalose Toxicity in Cuscuta reflexa: SUCROSE CONTENT DECREASES IN SHOOT TIPS UPON TREHALOSE FEEDING.

    abstract::Trehalose, an alpha,alpha-diglucoside, induced a rapid blackening and death of shoot tips of Cuscuta reflexa (dodder) cultured in vitro. The onset of toxic symptom was delayed if any of the several sugars which support the in vitro growth of Cuscuta was supplied with trehalose. The rate of trehalose uptake or its accu...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.69.6.1247

    authors: Veluthambi K,Mahadevan S,Maheshwari R

    更新日期:1982-06-01 00:00:00

  • Analysis of the Nicotiana tabacum stigma/style transcriptome reveals gene expression differences between wet and dry stigma species.

    abstract::The success of plant reproduction depends on pollen-pistil interactions occurring at the stigma/style. These interactions vary depending on the stigma type: wet or dry. Tobacco (Nicotiana tabacum) represents a model of wet stigma, and its stigmas/styles express genes to accomplish the appropriate functions. For a larg...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.131573

    authors: Quiapim AC,Brito MS,Bernardes LA,Dasilva I,Malavazi I,DePaoli HC,Molfetta-Machado JB,Giuliatti S,Goldman GH,Goldman MH

    更新日期:2009-03-01 00:00:00

  • Failure to Maintain Acetate Homeostasis by Acetate-Activating Enzymes Impacts Plant Development.

    abstract::The metabolic intermediate acetyl-CoA links anabolic and catabolic processes and coordinates metabolism with cellular signaling by influencing protein acetylation. In this study we demonstrate that in Arabidopsis (Arabidopsis thaliana), two distinctly localized acetate-activating enzymes, ACETYL-COA SYNTHETASE (ACS) i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.01162

    authors: Fu X,Yang H,Pangestu F,Nikolau BJ

    更新日期:2020-03-01 00:00:00

  • Analysis of Chromophytic and Rhodophytic Ribulose-1,5-Bisphosphate Carboxylase Indicates Extensive Structural and Functional Similarities among Evolutionarily Diverse Algae.

    abstract::Ribulose-1,5-bisphosphate carboxylase (Rubisco) from the algae Olisthodiscus luteus (chromophyte) and Griffithsia pacifica (rhodophyte) are remarkably similar to each other. However, both enzymes differ significantly in the structure and function when compared to Rubisco from green algae and land plants. Analysis of p...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.91.3.939

    authors: Newman SM,Derocher J,Cattolico RA

    更新日期:1989-11-01 00:00:00