Abstract:
:Soft x-ray microscopy (SXM) is a minimally invasive technique for single-cell high-resolution imaging as well as the visualization of intracellular distributions of light elements such as carbon, nitrogen, and oxygen. We used SXM to observe photosynthesis and nitrogen fixation in the filamentous cyanobacterium Anabaena sp. PCC 7120, which can form heterocysts during nitrogen starvation. Statistical and spectroscopic analyses from SXM images around the K-absorption edge of nitrogen revealed a significant difference in the carbon-to-nitrogen (C/N) ratio between vegetative cells and heterocysts. Application of this analysis to soft x-ray images of Anabaena sp. PCC 7120 revealed inhomogenous C/N ratios in the cells. Furthermore, soft x-ray tomography of Anabaena sp. PCC 7120 revealed differing cellular C/N ratios, indicating different carbon and nitrogen distributions between vegetative cells and heterocysts in three dimensions.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Teramoto T,Azai C,Terauchi K,Yoshimura M,Ohta Tdoi
10.1104/pp.17.01767subject
Has Abstractpub_date
2018-05-01 00:00:00pages
52-61issue
1eissn
0032-0889issn
1532-2548pii
pp.17.01767journal_volume
177pub_type
杂志文章相关文献
PLANT PHYSIOLOGY文献大全abstract::Using isopycnic sucrose gradients, we have ascertained the subcellular location of several enzymes involved in the processing of the N-linked oligosaccharides of glycoproteins in developing cotyledons of the common bean, Phaseolus vulgaris. All are localized in the endoplasmic reticulum (ER) or Golgi complex as determ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.85.3.741
更新日期:1987-11-01 00:00:00
abstract::Tobacco (Nicotiana tabacum L. cv Xanthi) plants were exposed to toxic levels of zinc (Zn). Zn exposure resulted in toxicity signs in plants, and these damages were partly reduced by a calcium (Ca) supplement. Confocal imaging of intracellular Zn using Zinquin showed that Zn was preferentially accumulated in trichomes....
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.106.082743
更新日期:2006-07-01 00:00:00
abstract::(125)I-Labeled ethylene biosynthesis-inducing xylanase (EIX) was used to study the movement of this protein in tobacco (Nicotiana tabacum) tissues. A biologically active (125)I-labeled EIX was obtained using chloramine-T as the oxidizing agent. Labeled EIX was detected in the far most edges of the leaf 5 min after it ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.100.4.2059
更新日期:1992-12-01 00:00:00
abstract::Primary leaves of 8-day-old Phaseolus vulgaris plants dipped in water at 46.5 to 47.5 C for 2 minutes were found to have decreased synthesis of lipid and beta-glucan compared to controls; UDP-glucose was used as substrate by cell-free preparations. As time elapsed, leaf growth, which was inhibited within the first 12 ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.53.1.118
更新日期:1974-01-01 00:00:00
abstract::Changes in the levels of twenty-two free amino acids and in the amino acid composition of the total protein were measured throughout the development of cotyledons of a dwarf garden pea, Pisum sativum cv Greenfeast, grown in a constant environment. A sensitive double-isotope dansylation technique was used. Fresh weight...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.2.492
更新日期:1983-06-01 00:00:00
abstract::Intact spheroplasts of the cyanobacterium (blue-green alga) Anacystis nidulans oxidized various exogenous c-type cytochromes with concomitant outward proton translocation while exogenous ferricytochrome c was not reduced. The H(+)/e(-) stoichiometry was close to 1 with each of the cytochromes and did not depend on the...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.75.4.968
更新日期:1984-08-01 00:00:00
abstract::The accumulation of starch within photosynthetic tissues and within dedicated storage organs has been characterized extensively in many species, and a function in buffering carbon availability or in fueling later growth phases, respectively, has been proposed. However, developmentally regulated starch turnover within ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.16.00916
更新日期:2016-12-01 00:00:00
abstract::DNA polymerase zeta catalytic subunit REV3 is known to play an important role in the repair of DNA damage induced by cross-linking and methylating agents. Here, we demonstrate that in Arabidopsis (Arabidopsis thaliana), the basic polymerase activity of REV3 is essential for resistance protection against these differen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.15.00806
更新日期:2015-12-01 00:00:00
abstract::Polyamines are small ubiquitous molecules that have been involved in nearly all developmental processes, including the stress response. Nevertheless, no direct evidence of a role of polyamines in the wound response has been described. We have studied the expression of genes involved in polyamine biosynthesis in respon...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.009951
更新日期:2002-11-01 00:00:00
abstract::Regulation of cortical microtubule reorganization is essential for plant cell survival under high salinity conditions. In response to salt stress, microtubules undergo rapid depolymerization followed by reassembly to form a new microtubule network that promotes cell survival; however, the upstream regulatory mechanism...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.17.01124
更新日期:2018-03-01 00:00:00
abstract::Experiments were conducted in growth chambers to examine the effect of a mild water stress (-200 kilopascals polyethylene glycol) on frond elongation and water status of the ostrich fern (Matteuccia struthiopteris [L.] Todaro). Measurements were taken for two days, starting one day after the application of polyethylen...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.72.1.96
更新日期:1983-05-01 00:00:00
abstract::A number of plants have been surveyed with respect to isolation by mild grinding in large quantities of leaf cells. The extent of recovery of mesophyll cells per unit leaf area was found to vary with plant species and the method of grinding. Greater than 70% recovery was obtained from the leaves of Canna indica L., Cr...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.54.4.569
更新日期:1974-10-01 00:00:00
abstract::The rhythmic movement of excised Samanea saman pulvini incubated in H(2)O or 50 mm sucrose was monitored during extended periods of white light (cool white fluorescent, 2,000 ft-c), darkness, or alternating white light (16 hr) and darkness (8 hr). In continuous white light, the rhythm damps at an intermediate angle af...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.58.3.417
更新日期:1976-09-01 00:00:00
abstract::The circadian RNA-binding protein CHLAMY1 from the green alga Chlamydomonas reinhardtii consists of two subunits named C1 and C3. Changes in the C1 level cause arrhythmicity of the phototaxis rhythm, while alterations in the level of C3 lead to acrophase shifts. Thus, CHLAMY1 is involved in maintaining period and phas...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.118570
更新日期:2008-08-01 00:00:00
abstract::The possible role of the octadecanoid signaling pathway with jasmonic acid (JA) as the central component in defense-gene regulation of pathogen-attacked rice was studied. Rice (Oryza sativa L.) seedlings were treated with JA or inoculated with the rice blast fungus Magnaporthe grisea (Hebert) Barr., and gene-expressio...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.1.79
更新日期:1997-05-01 00:00:00
abstract::Ion transport, measured using double-barreled micropipettes to obtain current-voltage relations, was examined in Arabidopsis thaliana root hairs that continued tip growth and cytoplasmic streaming after impalement with the micropipette. To do this required in situ measurements with no handling of the seedlings to avoi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.97.4.1527
更新日期:1991-12-01 00:00:00
abstract::The synthetically produced fluorescent siderophore NBD-desferrioxamine B (NBD-DFO), an analog of the natural siderophore ferrioxamine B, was used to study iron uptake by plants. Short-term (10-hour) (55)Fe uptake rates by cotton (Gossypium spp.) and maize (Zea mays L.) plants from the modified siderophore were similar...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.99.4.1329
更新日期:1992-08-01 00:00:00
abstract::The effects of the diphenyl ether herbicides HOE 29152 (methyl-2[4-(4-trifluoromethoxy) phenoxy] propanoate) and nitrofluorfen (2-chloro-1-[4-nitrophenoxy]-4-[trifluoromethyl]benzene) on photosynthetic electron transport have been examined with pea seedling and spinach chloroplasts. Linear electron transport (water to...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.65.1.47
更新日期:1980-01-01 00:00:00
abstract::Injection of [(14)C]tryptophan into one seed in a soybean fruit resulted in recovery of radiolabel in a fraction that cochromatographed with indoleacetic acid (IAA) through three successive high performance liquid chromatography separations. Label was found in the putative IAA fraction in all of the fruit tissues samp...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.82.2.454
更新日期:1986-10-01 00:00:00
abstract::In higher plants, blue light (BL) phototropism is primarily controlled by the phototropins, which are also involved in stomatal movement and chloroplast relocation. These photoresponses are mediated by two phototropins, phot1 and phot2. Phot1 mediates responses with higher sensitivity than phot2, and phot2 specificall...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.113.232306
更新日期:2014-04-01 00:00:00
abstract::Recent structure-function analysis of heterologously expressed K+-selective inward-rectifying channels (KIRCs) from plants has revealed that external protons can have opposite effects on different members of the same gene family. An important question is how the diverse response of KIRCs to apoplastic pH is reflected ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.120.1.331
更新日期:1999-05-01 00:00:00
abstract::Rice (Oryza sativa) is the staple food for over half the world's population yet may represent a significant dietary source of inorganic arsenic (As), a nonthreshold, class 1 human carcinogen. Rice grain As is dominated by the inorganic species, and the organic species dimethylarsinic acid (DMA). To investigate how As ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.109.146126
更新日期:2010-01-01 00:00:00
abstract::Tomato Atypical Receptor Kinase 1 (TARK1) is a pseudokinase required for postinvasion immunity. TARK1 was originally identified as a target of the Xanthomonas euvesicatoria effector protein Xanthomonas outer protein N (XopN), a suppressor of early defense signaling. How TARK1 participates in immune signal transduction...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.19.01400
更新日期:2020-07-01 00:00:00
abstract::Plant hormones modulate plant growth, development, and defense. However, many aspects of the origin and evolution of plant hormone signaling pathways remain obscure. Here, we use a comparative genomic and phylogenetic approach to investigate the origin and evolution of nine major plant hormone (abscisic acid, auxin, b...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.114.247403
更新日期:2015-03-01 00:00:00
abstract::Phosphoenolpyruvate carboxylase was purified from corn root tips about 80-fold by centrifugation, ammonium sulfate fractionation, and anion exchange and gel filtration chromatography. The resulting preparation was essentially free from malate dehydrogenase, isocitrate dehydrogenase, malate enzyme, NADH oxidase, and py...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.43.12.1919
更新日期:1968-12-01 00:00:00
abstract::Increasing experimental evidence suggests that the tricarboxylic acid cycle in plants is of greater importance in illuminated photosynthetic tissues than previously thought. In this study, transgenic tomato (Solanum lycopersicum) plants expressing a fragment of the beta-subunit of succinyl-coenzyme A ligase in either ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.107.103101
更新日期:2007-11-01 00:00:00
abstract::d-Galactose has been shown to have toxic and growth inhibitory effects in plants. When applied at levels of 50 millimolar to tobacco (Nicotiana tabacum L. cv Xanthi) leaf discs galactose caused a rapid increase in ethylene production during the first 2 days of incubation, followed by a rapid return to the basal level ...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.83.1.8
更新日期:1987-01-01 00:00:00
abstract::In angiosperms, auxin phytohormones play a crucial regulatory role in fruit initiation. The expression of auxin biosynthesis genes in ovules and placenta results in uncoupling of tomato (Solanum lycopersicum) fruit development from fertilization with production of parthenocarpic fruits. We have identified two newly de...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.108.131367
更新日期:2009-01-01 00:00:00
abstract::The influence of polyploidization on chloroplast coupling factor 1 (CF1) was examined in leaves and leaf protoplasts from isogenic diploid-tetraploid (DDC 2X-4X) and tetraploid-octoploid (IC 4X-8X) sets of alfalfa (Medicago sativa L.). Alfalfa CF1 was purified to homogeneity and was found to contain five subunits with...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.70.6.1715
更新日期:1982-12-01 00:00:00
abstract::Crown galls induced by infection of tobacco plants with Agrobacterium tumefaciens strain C58-Cl(pTiB6S3) were excised and cultured in vitro. After about one year of culture on medium-lacking phytohormones, two noncloned lines spontaneously formed shoots. Leaf explants from shoots of tumor-line T5 were capable of growi...
journal_title:Plant physiology
pub_type: 杂志文章
doi:10.1104/pp.69.1.37
更新日期:1982-01-01 00:00:00