Abstract:
:A number of plants have been surveyed with respect to isolation by mild grinding in large quantities of leaf cells. The extent of recovery of mesophyll cells per unit leaf area was found to vary with plant species and the method of grinding. Greater than 70% recovery was obtained from the leaves of Canna indica L., Crotalaria Laburnifolia L., and Thunbergia grandiflora Roxb.By pulse-chase time course experiments, the photosynthetically fixed primary carbon compounds of bean leaf cells were not converted into the ethanol-insoluble fraction. About 25% of total (14)C-photoassimilates were found to leak out into the incubation medium. In contrast, Euglena and Chlorella cells incorporated their primary photosynthetic products into cellular macromolecules and the amount of "leak" was very little. (14)C-Leucine supplied to the bean cells was absorbed readily and incorporated into the trichloroacetic acid insoluble fraction.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Kulandaivelu G,Gnanam Adoi
10.1104/pp.54.4.569subject
Has Abstractpub_date
1974-10-01 00:00:00pages
569-74issue
4eissn
0032-0889issn
1532-2548journal_volume
54pub_type
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