Abstract:
:Bean (Phaseolus vulgaris L.) seeds contain a putative plant defense protein that inhibits insect and mammalian but not plant alpha-amylases. We recently (J Moreno, MJ Chrispeels [1989] Proc Natl Acad Sci USA 86:7885-7889) presented strong circumstantial evidence that this alpha-amylase inhibitor (alphaAI) is encoded by an already-identified lectin gene whose product is referred to as lectin-like-protein (LLP). We have now made a chimeric gene consisting of the coding sequence of the lectin gene that encodes LLP and the 5' and 3' flanking sequences of the lectin gene that encodes phytohemagglutinin-L. When this chimeric gene was expressed in transgenic tobacco (Nicotiana tabacum), we observed in the seeds a series of polypeptides (M(r) 10,000-18,000) that cross-react with antibodies to the bean alpha-amylase inhibitor. Most of these polypeptides bind to a pig pancreas alpha-amylase affinity column. An extract of the seeds of the transformed tobacco plants inhibits pig pancreas alpha-amylase activity as well as the alpha-amylase present in the midgut of Tenebrio molitor. We suggest that introduction of this lectin gene (to be called alphaai) into other leguminous plants may be a strategy to protect the seeds from the seed-eating larvae of Coleoptera.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Altabella T,Chrispeels MJdoi
10.1104/pp.93.2.805subject
Has Abstractpub_date
1990-06-01 00:00:00pages
805-10issue
2eissn
0032-0889issn
1532-2548journal_volume
93pub_type
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