Bound auxin formation in growing stems.

Abstract:

:The term "bound auxin" is herein used to describe auxin conjugates insoluble in organic solvents which dissolve indoleacetic acid (IAA) and its derivatives, but hydrolyzable by NaOH to release IAA. Bound auxin from pea stems was fractionated into water-soluble, water-insoluble/NaOH-hydrolyzable, and insoluble components. Formation of bound auxin commenced with 15 minutes of applying exogenous labeled IAA, and progressively increased in amount, relative to IAA uptake, over 6 hours. Formation was not restricted to any particular zone of the stem and occurred in both light- and dark-grown stems. A greater quantity of bound auxin was formed in light-grown stems, reaching 4.2 and 7.7%, of the IAA taken up, in the water-soluble and water-insoluble/NaOH-hydrolyzable fractions after 6 hours. The presence of sucrose, during either the IAA treatment or an aging pretreatment had no effect, though 6 hours aging did cause a subsequent increase in the water-insoluble fraction of the bound auxin. Bound auxin formation in light-grown stems was dependent on respiratory metabolism, being reduced by KCN. It was also reduced, compared to total uptake, by inhibitors of RNA, and protein synthesis (6-methylpurine and cycloheximide) but only when the inhibitors preceded auxin addition and were present for a 4-hour period. Addition of inhibitors following auxin had no effect, suggesting an early inductive effect of auxin on bound auxin formation. Inhibitors of cell elongation had no effect. Deoxyglucose, an inhibitor of glucan synthesis, had only a small effect on the water-soluble fraction. Bound auxin is an important auxin product in growing plants. Its function is unknown, but some possibilities are discussed.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Davies PJ

doi

10.1104/pp.57.2.197

subject

Has Abstract

pub_date

1976-02-01 00:00:00

pages

197-202

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

57

pub_type

杂志文章
  • Effects of Nitrate Application on Amaranthus powellii Wats : II. Stomatal Response to Vapor Pressure Difference is Consistent with Optimization of Stomatal Conductance.

    abstract::The hypothesis of optimal stomatal conductance predicts conductance should vary with changes of the vapor pressure difference between leaf and air (VPD) to keep the partial derivative of transpiration rate (E) with respect to assimilation rate (A) constant ( partial differentialE/ partial differentialA = lambda). Stom...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.3.614

    authors: Hunt ER,Weber JA,Gates DM

    更新日期:1985-11-01 00:00:00

  • Emission of Hydrogen Sulfide by Leaf Tissue in Response to l-Cysteine.

    abstract::Leaf discs and detached leaves exposed to l-cysteine emitted a volatile sulfur compound which was proven by gas chromatography to be H(2)S. This phenomenon was demonstrated in all nine species tested (Cucumis sativus, Cucurbita pepo, Nicotiana tabacum, Coleus blumei, Beta vulgaris, Phaseolus vulgaris, Medicago sativa,...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.2.430

    authors: Sekiya J,Schmidt A,Wilson LG,Filner P

    更新日期:1982-08-01 00:00:00

  • The circadian clock that controls gene expression in Arabidopsis is tissue specific.

    abstract::The expression of CHALCONE SYNTHASE (CHS) expression is an important control step in the biosynthesis of flavonoids, which are major photoprotectants in plants. CHS transcription is regulated by endogenous programs and in response to environmental signals. Luciferase reporter gene fusions showed that the CHS promoter ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.005405

    authors: Thain SC,Murtas G,Lynn JR,McGrath RB,Millar AJ

    更新日期:2002-09-01 00:00:00

  • Development of the Potential for Cyanogenesis in Maturing Black Cherry (Prunus serotina Ehrh.) Fruits.

    abstract::Biochemical changes related to cyanogenesis (hydrogen cyanide production) were monitored during maturation of black cherry (Prunus serotina Ehrh.) fruits. At weekly intervals from flowering until maturity, fruits (or selected parts thereof) were analyzed for (a) fresh and dry weights, (b) prunasin and amygdalin levels...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.98.4.1423

    authors: Swain E,Li CP,Poulton JE

    更新日期:1992-04-01 00:00:00

  • beta-Conglycinins in Developing Soybean Seeds.

    abstract::The temporal sequence of development of the major proteins of seeds of soybean (Merr.) has been studied during development of cotyledons from flowering to maturity. A well-defined difference occurred in the times of appearance and the periods of maximum accumulation of alpha, alpha'-, and beta-subunits of betaconglyci...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.5.958

    authors: Gayler KR,Sykes GE

    更新日期:1981-05-01 00:00:00

  • Postharvest Variation in Cell Wall-Degrading Enzymes of Papaya (Carica papaya L.) during Fruit Ripening.

    abstract::Pectin methylesterase (PME), polygalacturonase (PG), xylanase, cellulase, and proteinase activity were determined and related to respiration, ethylene evolution, and changes in skin color of papaya (Carica papaya L.) fruit from harvest through to the start of fruit breakdown. PME gradually increased from the start of ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.72.2.382

    authors: Paull RE,Chen NJ

    更新日期:1983-06-01 00:00:00

  • Ion channels in Arabidopsis plasma membrane : transport characteristics and involvement in light-induced voltage changes.

    abstract::White light (25 watts per square meter) induced an increase in plasma membrane K(+)-channel activity and a 30- to 70-millivolt transient membrane depolarization (completed in 2-3 minutes) in Arabidopsis thaliana leaf mesophyll cells. Transport characteristics of three types of ion channels in the plasma membrane were ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.1.96

    authors: Spalding EP,Slayman CL,Goldsmith MH,Gradmann D,Bertl A

    更新日期:1992-05-01 00:00:00

  • Ricinosomes predict programmed cell death leading to anther dehiscence in tomato.

    abstract::Successful development and dehiscence of the anther and release of pollen are dependent upon the programmed cell death (PCD) of the tapetum and other sporophytic tissues. Ultrastructural examination of the developing and dehiscing anther of tomato (Solanum lycopersicum) revealed that cells of the interlocular septum, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.108.132720

    authors: Senatore A,Trobacher CP,Greenwood JS

    更新日期:2009-02-01 00:00:00

  • Ploidy effects on anatomy and gas exchange of tall fescue leaves.

    abstract::A growth chamber study was designed to interpret differences in CO(2) exchange rate (CER) and leaf diffusive resistance among 4X, 6X, 8X, and 10X ploidy levels of tall fescue (Festuca arundinacea, Schreb). Mesophyll cell size, stomatal density, number of major and minor veins, and bundle cap size of leaf blades were e...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.4.891

    authors: Byrne MC,Nelson CJ,Randall DD

    更新日期:1981-10-01 00:00:00

  • Identification of microRNAs involved in pathogen-associated molecular pattern-triggered plant innate immunity.

    abstract::Pathogen-associated molecular patterns (PAMPs) trigger plant defenses when perceived by surface-localized immune receptors. PAMP-triggered immunity (PTI) plays a vital role in the resistance of plants to numerous potential pathogens. MicroRNA (miRNA) biogenesis is known to be important for PTI, but miRNA species invol...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.151803

    authors: Li Y,Zhang Q,Zhang J,Wu L,Qi Y,Zhou JM

    更新日期:2010-04-01 00:00:00

  • Temperature-induced fluorescence changes : a screening method for frost tolerance of potato (solanum sp.).

    abstract::Field-grown tuber-bearing potatoes were screened for frost tolerance in a late stage of development. Three different clones of Solanum tuberosum L. and two interspecific crosses between clones of S. tuberosum and the wild potato species S. demissum Lindl. were studied. Two different methods were used. (a) Temperature-...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.70.5.1299

    authors: Sundbom E,Strand M,Hällgren JE

    更新日期:1982-11-01 00:00:00

  • Subcellular Distribution of Enzymes of Glycolate Metabolism in the Alga Cyanidium caldarium.

    abstract::The intracellular distribution of enzymes capable of catalyzing the reactions from phosphoglycolate to glycerate in the bluegreen colored eucaryotic alga Cyanidium caldarium has been studied. After separating the organelles from a crude homogenate on a linear flotation gradient, the enzymes glycolate oxidase and gluta...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.3.799

    authors: Gross W,Beevers H

    更新日期:1989-07-01 00:00:00

  • Effects of CO(2) and O(2) on Photosynthesis and Growth of Autotrophic Tobacco Callus.

    abstract::Mean inhibition of net photosynthesis in autotrophic tobacco callus by 21 and 40% O(2) was 30 and 47%, respectively, similar to intact leaves. Increasing CO(2) concentrations (500-2000 microliters per liter) produced a steady decline in percent inhibition at both O(2) concentrations, indicating that O(2) inhibition re...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.4.1055

    authors: McHale NA,Zelitch I,Peterson RB

    更新日期:1987-08-01 00:00:00

  • The hpa1 mutant of Arabidopsis reveals a crucial role of histidine homeostasis in root meristem maintenance.

    abstract::Histidine (His) is an essential ingredient for protein synthesis and is required by all living organisms. In higher plants, although there is considerable evidence that His is essential for plant growth and survival, there is very little information as to whether it plays any specific role in plant development. Here, ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.084178

    authors: Mo X,Zhu Q,Li X,Li J,Zeng Q,Rong H,Zhang H,Wu P

    更新日期:2006-08-01 00:00:00

  • Remodeling of Chlamydomonas Metabolism Using Synthetic Inducers Results in Lipid Storage during Growth.

    abstract::Microalgae accumulate lipids during stress such as that of nutrient deprivation, concomitant with cessation of growth and depletion of chloroplasts. By contrast, certain small chemical compounds selected by high-throughput screening in Chlamydomonas reinhardtii can induce lipid accumulation during growth, maintaining ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.19.00758

    authors: Wase N,Tu B,Rasineni GK,Cerny R,Grove R,Adamec J,Black PN,DiRusso CC

    更新日期:2019-11-01 00:00:00

  • A novel method of natural cryoprotection : intracellular glass formation in deeply frozen populus.

    abstract::Correlating measurements from differential scanning calorimetry, freeze-fracture freeze-etch electron microscopy, and survival of twigs after two-step cooling experiments, we provide strong evidence that winter-hardened Populus balsamifera v. virginiana (Sarg.) resists the stresses of freezing below -28 degrees C by a...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.1.41

    authors: Hirsh AG,Williams RJ,Meryman HT

    更新日期:1985-09-01 00:00:00

  • Brittle-1, an adenylate translocator, facilitates transfer of extraplastidial synthesized ADP--glucose into amyloplasts of maize endosperms.

    abstract::Amyloplasts of starchy tissues such as those of maize (Zea mays L.) function in the synthesis and accumulation of starch during kernel development. ADP-glucose pyrophosphorylase (AGPase) is known to be located in chloroplasts, and for many years it was generally accepted that AGPase was also localized in amyloplasts o...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.4.1235

    authors: Shannon JC,Pien FM,Cao H,Liu KC

    更新日期:1998-08-01 00:00:00

  • Cytochemical localization of catalase activity in glyoxysomes from castor bean endosperm.

    abstract::Glyoxysomes isolated from castor bean (Ricinus communis L. var. zanzibariensis) endosperm have been stained by the cytochemical diaminobenzidine reaction. The reaction product obtained by preincubation with 3,3'-diaminobenzidine and incubation with the reagent and H(2)O(2) is distributed uniformly throughout the matri...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.53.2.276

    authors: Bieglmayer C,Ruis H

    更新日期:1974-02-01 00:00:00

  • Inhibition of Barnyardgrass 4-Hydroxyphenylpyruvate Dioxygenase by Sulcotrione.

    abstract::4-Hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) was partially purified from barnyardgrass (Echinochloa crus-galli L.) leaves and assayed by high-performance liquid chromatography analysis of product formation or by the capture of released 14CO2. The bleaching herbicide sulcotrione [2-(2-chloro-4-methanesulfonylben...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.4.1429

    authors: Secor J

    更新日期:1994-12-01 00:00:00

  • Identification of a specific isoform of tomato lipoxygenase (TomloxC) involved in the generation of fatty acid-derived flavor compounds.

    abstract::There are at least five lipoxygenases (TomloxA, TomloxB, TomloxC, TomloxD, and TomloxE) present in tomato (Lycopersicon esculentum Mill.) fruit, but their role in generation of fruit flavor volatiles has been unclear. To assess the physiological role of TomloxC in the generation of volatile C6 aldehyde and alcohol fla...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.041608

    authors: Chen G,Hackett R,Walker D,Taylor A,Lin Z,Grierson D

    更新日期:2004-09-01 00:00:00

  • TOPP4 Regulates the Stability of PHYTOCHROME INTERACTING FACTOR5 during Photomorphogenesis in Arabidopsis.

    abstract::In plants, photoreceptors transfer light signals to phytochrome-interacting factors (PIFs), inducing the rapid phosphorylation and degradation of PIFs to promote photomorphogenesis. However, the phosphatase responsible for PIF dephosphorylation remains unknown. In this study, we identified a type 1 protein phosphatase...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.01729

    authors: Yue J,Qin Q,Meng S,Jing H,Gou X,Li J,Hou S

    更新日期:2016-03-01 00:00:00

  • Mechanical stimuli modulate lateral root organogenesis.

    abstract::Unlike mammals, whose development is limited to a short temporal window, plants produce organs de novo throughout their lifetime in order to adapt their architecture to the prevailing environmental conditions. The production of lateral roots represents one example of such postembryonic organogenesis. An endogenous dev...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.142448

    authors: Richter GL,Monshausen GB,Krol A,Gilroy S

    更新日期:2009-12-01 00:00:00

  • Cell-Wall Proteins Induced by Water Deficit in Bean (Phaseolus vulgaris L.) Seedlings.

    abstract::In the last few years, much attention has been given to the role of proteins that accumulate during water deficit. In this work, we analyzed the electrophoretic patterns of basic protein extracts, enriched for a number of cell-wall proteins, from bean (Phaseolus vulgaris L.) seedlings and 21-d-old plants subjected to ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.107.4.1119

    authors: Covarrubias AA,Ayala JW,Reyes JL,Hernandez M,Garciarrubio A

    更新日期:1995-04-01 00:00:00

  • Sensitive Detection of Phosphorus Deficiency in Plants Using Chlorophyll a Fluorescence.

    abstract::Phosphorus (P) is a finite natural resource and an essential plant macronutrient with major impact on crop productivity and global food security. Here, we demonstrate that time-resolved chlorophyll a fluorescence is a unique tool to monitor bioactive P in plants and can be used to detect latent P deficiency. When plan...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00823

    authors: Frydenvang J,van Maarschalkerweerd M,Carstensen A,Mundus S,Schmidt SB,Pedas PR,Laursen KH,Schjoerring JK,Husted S

    更新日期:2015-09-01 00:00:00

  • Modification of cytokinins by cauliflower microsomal enzymes.

    abstract::N(6)-(Delta(2)-isopentenyl)Adenine and N(6)-(Delta(2)-isopentenyl)adenosine were hydroxylated, respectively, to 6-(4-hydroxy-3-methyl-trans-2-butenylamino)purine and 6-(4-hydroxy-3-methyl-trans-2-butenylamino) -9-beta-ribofuranosylpurine in the presence of NADPH and the microsomal fraction from cauliflowers (Brassica ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.75.2.442

    authors: Chen CM,Leisner SM

    更新日期:1984-06-01 00:00:00

  • A low-CO2-inducible gene encoding an alanine: alpha-ketoglutarate aminotransferase in Chlamydomonas reinhardtii.

    abstract::At low-CO2 (air) conditions, the unicellular green alga Chlamydomonas reinhardtii acquires the ability to raise its internal inorganic carbon concentration. To study this adaptation to low CO2, cDNA clones induced under low-CO2 growth conditions were selected through differential screening. One full-length clone is 25...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.2.677

    authors: Chen ZY,Burow MD,Mason CB,Moroney JV

    更新日期:1996-10-01 00:00:00

  • Photooxidative Destruction of Chloroplasts Leads to Reduced Expression of Peroxisomal NADH-Dependent Hydroxypyruvate Reductase in Developing Cucumber Cotyledons.

    abstract::Photooxidative destruction of chloroplasts by exposure of norflurazon-treated cucumber (Cucumis sativus L.) seedlings to white light leads to reduced levels of the nuclear-encoded, peroxisomal enzyme hydroxypyruvate reductase. The partial reduction in hydroxypyruvate reductase activity under photooxidative conditions ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.2.681

    authors: Schwartz BW,Daniel SG,Becker WM

    更新日期:1992-06-01 00:00:00

  • FLAVODIIRON2 and FLAVODIIRON4 proteins mediate an oxygen-dependent alternative electron flow in Synechocystis sp. PCC 6803 under CO2-limited conditions.

    abstract::This study aims to elucidate the molecular mechanism of an alternative electron flow (AEF) functioning under suppressed (CO2-limited) photosynthesis in the cyanobacterium Synechocystis sp. PCC 6803. Photosynthetic linear electron flow, evaluated as the quantum yield of photosystem II [Y(II)], reaches a maximum shortly...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.249987

    authors: Shimakawa G,Shaku K,Nishi A,Hayashi R,Yamamoto H,Sakamoto K,Makino A,Miyake C

    更新日期:2015-02-01 00:00:00

  • Nod factors induce nod factor cleaving enzymes in pea roots. Genetic and pharmacological approaches indicate different activation mechanisms.

    abstract::Establishment of symbiosis between legumes and rhizobia requires bacterial Nod factors (NFs). The concentration of these lipochitooligosaccharides in the rhizosphere is influenced by plant enzymes. NFs induce on pea (Pisum sativum) a particular extracellular NF hydrolase that releases lipodisaccharides from NFs from S...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.105.061705

    authors: Ovtsyna AO,Dolgikh EA,Kilanova AS,Tsyganov VE,Borisov AY,Tikhonovich IA,Staehelin C

    更新日期:2005-10-01 00:00:00

  • DRT111/SFPS Splicing Factor Controls Abscisic Acid Sensitivity during Seed Development and Germination.

    abstract::RNA splicing is a fundamental mechanism contributing to the definition of the cellular protein population in any given environmental condition. DNA-DAMAGE REPAIR/TOLERATION PROTEIN111 (DRT111)/SPLICING FACTOR FOR PHYTOCHROME SIGNALING is a splicing factor previously shown to interact with phytochrome B and characteriz...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00037

    authors: Punzo P,Ruggiero A,Possenti M,Perrella G,Nurcato R,Costa A,Morelli G,Grillo S,Batelli G

    更新日期:2020-06-01 00:00:00