Modification of cytokinins by cauliflower microsomal enzymes.

Abstract:

:N(6)-(Delta(2)-isopentenyl)Adenine and N(6)-(Delta(2)-isopentenyl)adenosine were hydroxylated, respectively, to 6-(4-hydroxy-3-methyl-trans-2-butenylamino)purine and 6-(4-hydroxy-3-methyl-trans-2-butenylamino) -9-beta-ribofuranosylpurine in the presence of NADPH and the microsomal fraction from cauliflowers (Brassica oleracea L.). The hydroxylating reaction was completely inhibited by 10 millimolars metyrapone and partially inactivated by 10-minute treatment of the microsomal preparation with ethylene. The cytokinins were also dealkylated by the microsomal enzymes and formed adenine from cytokinin base and adenosine from cytokinin nucleoside. These results suggest that plant cytochrome P-450 is involved in the conversion of one type of cytokinin to another, and in the modification of cytokinin molecules.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Chen CM,Leisner SM

doi

10.1104/pp.75.2.442

subject

Has Abstract

pub_date

1984-06-01 00:00:00

pages

442-6

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

75

pub_type

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