Abstract:
:The mechanism of sucrose transport into vacuoles isolated from leaf tissue has been studied only in barley (Hordeum vulgare) mesophyll cells. In this tissue, sucrose transport was reported to be a facilitated diffusion. We have observed a facilitated diffusion of sucrose into vacuoles isolated from this tissue. However, no pH dependence was observed. Evidence is presented indicating that the pH dependence of sucrose uptake into vacuoles may be an artifact, reflecting tonoplast instability and survival of isolated vacuoles in different buffers. Apparently vacuoles do not withstand exposure to some commonly used buffers.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
De Leon JL,Daie J,Wyse Rdoi
10.1104/pp.88.2.251subject
Has Abstractpub_date
1988-10-01 00:00:00pages
251-4issue
2eissn
0032-0889issn
1532-2548journal_volume
88pub_type
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