Tonoplast stability and survival of isolated vacuoles in different buffers.

Abstract:

:The mechanism of sucrose transport into vacuoles isolated from leaf tissue has been studied only in barley (Hordeum vulgare) mesophyll cells. In this tissue, sucrose transport was reported to be a facilitated diffusion. We have observed a facilitated diffusion of sucrose into vacuoles isolated from this tissue. However, no pH dependence was observed. Evidence is presented indicating that the pH dependence of sucrose uptake into vacuoles may be an artifact, reflecting tonoplast instability and survival of isolated vacuoles in different buffers. Apparently vacuoles do not withstand exposure to some commonly used buffers.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

De Leon JL,Daie J,Wyse R

doi

10.1104/pp.88.2.251

subject

Has Abstract

pub_date

1988-10-01 00:00:00

pages

251-4

issue

2

eissn

0032-0889

issn

1532-2548

journal_volume

88

pub_type

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