Expression of DNA coding for diphtheria toxin chain a is toxic to plant cells.

Abstract:

:DNA coding for the enzymatically active subunit A of diphtheria toxin was placed under the control of the cauliflower mosaic virus 35S promoter and the Agrobacterium left transfer-DNA gene 7 polyadenylation signal. Agrobacteria carrying a binary plant vector with the chimeric diphtheria toxin A gene had very low transforming activity in tobacco (Nicotiana tabacum L.), and greatly diminished the recovery of stable transformants when mixed together with agrobacteria which alone transformed plant cells well. The introduction of this chimeric molecule into tobacco cells by electroporation lowered the level of the transient expression of the coelectroporated chloramphenicol acetyltransferase reporter gene indicating that expression of diphtheria toxin chain A in plant cells is toxic. We have developed a binary vector pGA987 which can be used for probing a variety of plant promoters.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Czako M,An G

doi

10.1104/pp.95.3.687

subject

Has Abstract

pub_date

1991-03-01 00:00:00

pages

687-92

issue

3

eissn

0032-0889

issn

1532-2548

journal_volume

95

pub_type

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