Glycoprotein Synthesis in Plants: II. Structure of the Mannolipid Intermediate.

Abstract:

:This paper reports the purification and structural determination of the mannolipid shown previously (Ericson and Delmer 1977 Plant Physiol 59: 341-347) to serve as an intermediate in glycoprotein synthesis in cotyledons of Phaseolus vulgaris. The mannolipid was purified by chromatography in organic solvents on diethylaminoethyl-cellulose, followed by repeated steps of deacylation and rechromatography on Sephadex LH-20. Binding and elution behavior on diethylaminoethyl-cellulose was consistent with the presence of a monophosphate residue. Lability of the mannolipid to mild acid treatment as well as its resistance to hot phenol treatment or catalytic hydrogenation are consistent with the structure of a polyprenol having a saturated alpha-residue. After methanolysis, the chloroform-methanol-soluble portion of the mannolipid was analyzed by mass spectrometry. The fragmentation pattern obtained was nearly identical to that obtained from standard dolichol-phosphate. An intense ion at m/e 69 represented the omega-terminal isoprenoid residue, and repeating fragments separated by 68 mass units were observed up to m/e of > 1,200. All evidence supports the conclusion that the mannolipid is dolichol-monophosphate-mannose and thus provides further support for the concept that the processes involved in the glycosylation of protein in higher plants are similar to those known to occur in the animal kingdom.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Delmer DP,Kulow C,Ericson MC

doi

10.1104/pp.61.1.25

subject

Has Abstract

pub_date

1978-01-01 00:00:00

pages

25-9

issue

1

eissn

0032-0889

issn

1532-2548

journal_volume

61

pub_type

杂志文章
  • Metabolism of C-Maltose in Avena fatua Seeds During Germination.

    abstract::Non-dormant and dormant seeds of Avena fatua metabolize (14)C-maltose in different ways: in non-dormant seeds, (14)C-maltose administered to the endosperm is readily converted to sucrose in the scutellum and translocated to the embryo; in dormant seeds, little sucrose is synthesized from (14)C-maltose, and maltose and...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.5.770

    authors: Chen SS,Varner JE

    更新日期:1969-05-01 00:00:00

  • Reversible o(2) inhibition of nitrogenase activity in attached soybean nodules.

    abstract::Various forms of stress result in decreased O(2) permeability or decreased capacity to consume O(2) in legume root nodules. These changes alter the nodule interior O(2) concentration (O(i)). To determine the relationship between O(i) and nitrogenase activity in attached soybean (Glycine max) nodules, we controlled O(i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.4.1863

    authors: Denison RF,Witty JF,Minchin FR

    更新日期:1992-12-01 00:00:00

  • On the uptake, metabolism and retention of [h] gibberellin a(1) by barley aleurone layers at low temperatures.

    abstract::Barley (c.v. Himalaya) aleurone layers were incubated in [(3)H]gibberellin A(1) (GA(1)) at low temperatures. At 3 and 4 C, (3)H-activity was steadily accumulated in aleurone layers, and this accumulation was correlated with significant [(3)H]GA(1) metabolism. At 1 and 1.5 C, metabolism could not be detected, and at th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.66.5.956

    authors: Keith B,Boal R,Srivastava LM

    更新日期:1980-11-01 00:00:00

  • DRT111/SFPS Splicing Factor Controls Abscisic Acid Sensitivity during Seed Development and Germination.

    abstract::RNA splicing is a fundamental mechanism contributing to the definition of the cellular protein population in any given environmental condition. DNA-DAMAGE REPAIR/TOLERATION PROTEIN111 (DRT111)/SPLICING FACTOR FOR PHYTOCHROME SIGNALING is a splicing factor previously shown to interact with phytochrome B and characteriz...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.20.00037

    authors: Punzo P,Ruggiero A,Possenti M,Perrella G,Nurcato R,Costa A,Morelli G,Grillo S,Batelli G

    更新日期:2020-06-01 00:00:00

  • Properties of Ornithine Carbamoyltransferase from Pisum sativum L.

    abstract::Some properties of ornithine carbamoyltransferase from chloroplasts isolated from leaves of Pisum sativum L. (cv Marzia) were compared with those of the enzyme partially purified (316-fold) from shoots of seedlings after 3 weeks of cultivation.Both preparations showed a pH optimum at pH 8.3 and had the same affinity t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.77.3.695

    authors: de Ruiter H,Kollöffel C

    更新日期:1985-03-01 00:00:00

  • Overexpression of ARGOS Genes Modifies Plant Sensitivity to Ethylene, Leading to Improved Drought Tolerance in Both Arabidopsis and Maize.

    abstract::Lack of sufficient water is a major limiting factor to crop production worldwide, and the development of drought-tolerant germplasm is needed to improve crop productivity. The phytohormone ethylene modulates plant growth and development as well as plant response to abiotic stress. Recent research has shown that modify...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.15.00780

    authors: Shi J,Habben JE,Archibald RL,Drummond BJ,Chamberlin MA,Williams RW,Lafitte HR,Weers BP

    更新日期:2015-09-01 00:00:00

  • Slow-growth phenotype of transgenic tomato expressing apoplastic invertase.

    abstract::The growth of transgenic tomato (Lycopersicon esculentum) plants that express in their apoplast yeast invertase under the control of the cauliflower mosaic virus 35S promoter is severely inhibited. The higher the level of invertase, the greater the inhibition of growth. A second phenotypic characteristic of these tran...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.95.2.420

    authors: Dickinson CD,Altabella T,Chrispeels MJ

    更新日期:1991-02-01 00:00:00

  • Rhythmic Differences in the Basipetal Movement of Indoleacetic Acid between Separated Upper and Lower Halves of Geotropically Stimulated Corn Coleoptiles.

    abstract::Rhythmic fluctuation in the basipetal movement of auxin occurs in corn (Zea mays) coleoptiles oriented either in the vertical or in the horizontal position. This periodicity of transport rate varies from region to region in a horizontal coleoptile. Between an upper and lower half coleoptile (with respect to gravity), ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.2.166

    authors: Shen-Miller J

    更新日期:1973-08-01 00:00:00

  • ESA1 Is Involved in Embryo Sac Abortion in Interspecific Hybrid Progeny of Rice.

    abstract::The emergence of sterile individuals in the hybrid backcross progeny of wild and cultivated rice limits the use of wild rice alleles for improving cultivated rice, but the molecular mechanisms underlying this sterility remain unclear. Here, we identified the semisterile introgression line YIL42, derived from a cross b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.18.01374

    authors: Hou J,Cao C,Ruan Y,Deng Y,Liu Y,Zhang K,Tan L,Zhu Z,Cai H,Liu F,Sun H,Gu P,Sun C,Fu Y

    更新日期:2019-05-01 00:00:00

  • Identification and Partial Characterization of the Denaturation Transition of the Photosystem II Reaction Center of Spinach Chloroplast Membranes.

    abstract::Sensitive differential scanning calorimetry was employed to investigate thylakoid membrane structure. Calorimetric scans of chloroplast membranes suspended in a low ionic strength Hepesbuffered medium revealed endothermic transitions centered at the following temperatures ( degrees C): A (42.5), B (60.6), C(1) (64.9),...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.2.575

    authors: Smith KA,Low PS

    更新日期:1989-06-01 00:00:00

  • Involvement of phytosulfokine in the attenuation of stress response during the transdifferentiation of zinnia mesophyll cells into tracheary elements.

    abstract::Phytosulfokine (PSK) is a sulfated peptide hormone required for the proliferation and differentiation of plant cells. Here, we characterize the physiological roles of PSK in transdifferentiation of isolated mesophyll cells of zinnia (Zinnia elegans 'Canary Bird') into tracheary elements (TEs). Transcripts for a zinnia...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.109.135954

    authors: Motose H,Iwamoto K,Endo S,Demura T,Sakagami Y,Matsubayashi Y,Moore KL,Fukuda H

    更新日期:2009-05-01 00:00:00

  • Purification and characterization of the reconstitutively active citrate carrier from maize mitochondria.

    abstract::The citrate carrier from maize (Zea mays) shoot mitochondria was solubilized with Triton X-100 and purified by sequential chromatography on hydroxyapatite and hydroxyapatite/celite in the presence of cardiolipin. SDS-gel electrophoresis of the purified fraction showed a single polypeptide band with an apparent molecul...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.120.3.841

    authors: Genchi G,Spagnoletta A,De Santis A,Stefanizzi L,Palmieri F

    更新日期:1999-07-01 00:00:00

  • Superoxide Dismutases: II. Purification and Quantitative Relationship with Water-soluble Protein in Seedlings.

    abstract::Superoxide dismutase was purified from pea (Pisum sativum L., cv. Wando) seeds and corn (Zea mays L., cv. Michigan 500) seedlings. The purified pea enzyme eluting as a single peak from gel exclusion chromatography columns contained the three electrophoretically distinct bands of superoxide dismutase characterizing the...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.2.315

    authors: Giannopolitis CN,Ries SK

    更新日期:1977-02-01 00:00:00

  • Fructosyl Transfer between 1-Kestose and Sucrose in Wheat Leaves.

    abstract::The labeling pattern of the sugar moieties of 1-kestose after in vivo pulse labeling with (14)CO(2) was not the same as that after in vitro labeling with (14)C-sucrose. The two fructosyl residues of 1-kestose had similar specific radioactivities after in vitro synthesis, but after in vivo radiolabeling the specific ra...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.96.1.251

    authors: Kanabus J,Gibeaut DM,Carpita NC,Housley TL

    更新日期:1991-05-01 00:00:00

  • Cytosolic triosephosphate isomerase is a single gene in rice.

    abstract::A cDNA clone encoding rice (Oryza sativa L.) cytosolic triosephosphate isomerase (TPI), an important glycolytic enzyme, was isolated and characterized. The clone (pRTPI-6) contains an open reading frame of 759 base pairs, encoding a polypeptide chain of 253 amino acid residues (M(r) 27,060). The identity of this clone...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.101.2.683

    authors: Xu Y,Hall TC

    更新日期:1993-02-01 00:00:00

  • Effects of low concentrations of o(3) on net photosynthesis, dark respiration, and chlorophyll contents in aging hybrid poplar leaves.

    abstract::Chronic exposure of hybrid poplar (Populus deltoides x trichocarpa) plants to low concentrations of ozone had negative impact upon net photosynthetic capacity, dark respiration, and leaf chlorophyll contents. Exposure to as much as 0.20 microliters per liter O(3) had no immediate effect on net photosynthesis (P(n)), b...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.73.2.291

    authors: Reich PB

    更新日期:1983-10-01 00:00:00

  • Complex I impairment, respiratory compensations, and photosynthetic decrease in nuclear and mitochondrial male sterile mutants of Nicotiana sylvestris.

    abstract::We have previously shown that in Nicotiana sylvestris cytoplasmic male-sterile (CMS) mutants where the mtDNA lacks the nad7 gene coding for a subunit of respiratory Complex I (NADH:ubiquinone oxidoreductase, EC 1.6.5.3), glycine (Gly) oxidation was lower than in the wild type and insensitive to rotenone, suggesting Co...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.124.3.1239

    authors: Sabar M,De Paepe R,de Kouchkovsky Y

    更新日期:2000-11-01 00:00:00

  • Drought Adaptation in Opuntia basilaris: Significance of Recycling Carbon through Crassulacean Acid Metabolism.

    abstract::Contrasting metabolic regimes operate in Opuntia basilaris Engelm. and Bigelov, before and after precipitation. During periods of drought, atmospheric CO(2) exchange and transpiration are greatly reduced throughout the day/night cycle by stomatal closure and a highly impervious cuticle. The hypothesis is that endogeno...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.6.539

    authors: Szarek SR,Johnson HB,Ting IP

    更新日期:1973-12-01 00:00:00

  • Water is allocated differently to chloroplasts in sun and shade leaves.

    abstract::Hydrogen-1 nuclear magnetic resonance spectroscopy was used to study water allocation in cell compartments of sun and shade leaves. NMR spectra of Acer platanoides were resolved into two peaks that were assigned to chloroplast and nonchloroplast water. Sun leaves contained 1.7 times more water per unit area of surface...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.86.1.16

    authors: McCain DC,Croxdale J,Markley JL

    更新日期:1988-01-01 00:00:00

  • Isolation and characterization of glutathione S-transferase isozymes from sorghum.

    abstract::Two glutathione S-transferase (GST) isozymes, A1/A1 and B1/B2, were purified from etiolated, O-1,3-dioxolan-2-yl-methyl-2,2,2, -trifluoro-4'-chloroacetophenone-oxime-treated sorghum (Sorghum bicolor L. Moench) shoots. GST A1/A1, a constitutively expressed homodimer, had a subunit molecular mass of 26 kD and an isoelec...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.3.877

    authors: Gronwald JW,Plaisance KL

    更新日期:1998-07-01 00:00:00

  • Posttranslational Modifications of Chloroplast Proteins: An Emerging Field.

    abstract::Posttranslational modifications of proteins are key effectors of enzyme activity, protein interactions, targeting, and turnover rate, but despite their importance, they are still poorly understood in plants. Although numerous reports have revealed the regulatory role of protein phosphorylation in photosynthesis, vario...

    journal_title:Plant physiology

    pub_type: 杂志文章,评审

    doi:10.1104/pp.15.00117

    authors: Lehtimäki N,Koskela MM,Mulo P

    更新日期:2015-07-01 00:00:00

  • Quantitative changes in in vitro and in vivo protein synthesis in aging and rejuvenated soybean cotyledons.

    abstract::Cotyledons of light-grown soybean (Glycine max L. var Wayne) seedlings were used as a model system to study the possibility that aging requires qualitative changes in protein synthesis. Cotyledons reached a final stage of senescence and then abscised about 22 days after imbibition. Cotyledon senescence was reversed at...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.4.861

    authors: Skadsen RW,Cherry JH

    更新日期:1983-04-01 00:00:00

  • The Stimulation of Ethylene Synthesis in Nicotiana tabacum Leaves by the Phytotoxin Coronatine.

    abstract::Coronatine is a chlorosis-inducing toxin produced by the plant pathogen Pseudomonas syringae pv atropurpurea. This bacterium is the causal agent of chocolate spot disease, in which brown lesions with chlorotic margins develop on the leaves of Lolium multiflorum Lam. Among the many physiological changes to plants cause...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.1.219

    authors: Kenyon JS,Turner JG

    更新日期:1992-09-01 00:00:00

  • Further characterization of ribosome binding to thylakoid membranes.

    abstract::Previous work indicated more polysomes bound to pea (Pisum sativum cv Progress No. 9) thylakoids in light than in the dark, in vivo (LE Fish, AT Jagendorf 1982 Plant Physiol 69: 814-825). With isolated intact chloroplasts incubated in darkness, addition of MgATP had no effect but 24 to 74% more RNA was thylakoid-bound...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.84.1.31

    authors: Hurewitz J,Jagendorf AT

    更新日期:1987-05-01 00:00:00

  • Extremely low frequency resistance fluctuations of chara membrane.

    abstract::The membrane resistivity of resting characean cells was measured and found to fluctuate unpredictably about its resting level. The amplitude of these fluctuations relative to the mean resistivity was normally somewhat larger than the same ratio for the vacuolar voltage; further the resistivity and voltage fluctuations...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.79.3.723

    authors: Gokhale AV,Pickard WF

    更新日期:1985-11-01 00:00:00

  • High genetic variability of herbivore-induced volatile emission within a broad range of maize inbred lines.

    abstract::Maize plants (Zea mays) attacked by caterpillars release a mixture of odorous compounds that attract parasitic wasps, natural enemies of the herbivores. We assessed the genetic variability of these induced volatile emissions among 31 maize inbred lines representing a broad range of genetic diversity used by breeders i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.039891

    authors: Degen T,Dillmann C,Marion-Poll F,Turlings TC

    更新日期:2004-08-01 00:00:00

  • Chemical Composition of Hypodermal and Endodermal Cell Walls and Xylem Vessels Isolated from Clivia miniata (Identification of the Biopolymers Lignin and Suberin).

    abstract::The occurrence of the biopolymers lignin and suberin was investigated with hypodermal (HCW) and endodermal cell walls (ECW) and xylem vessels (XV) isolated from Clivia miniata Reg. roots. Both biopolymers were detected in HCW and ECW, whereas in XV, typical aliphatic suberin monomers were missing and only representati...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.113.4.1223

    authors: Zeier J,Schreiber L

    更新日期:1997-04-01 00:00:00

  • Direct evidence for rapid degradation of Bacillus thuringiensis toxin mRNA as a cause of poor expression in plants.

    abstract::It is well established that the expression of Bacillus thuringiensis (B.t.) toxin genes in higher plants is severely limited at the mRNA level, but the cause remains controversial. Elucidating whether mRNA accumulation is limited transcriptionally or posttranscriptionally could contribute to effective gene design as w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.117.4.1445

    authors: De Rocher EJ,Vargo-Gogola TC,Diehn SH,Green PJ

    更新日期:1998-08-01 00:00:00

  • Adaptation to CO(2) Level and Changes in the Phosphorylation of Thylakoid Proteins during the Cell Cycle of Chlamydomonas reinhardtii.

    abstract::The photosynthetic performance of synchronously grown Chlamydomonas reinhardtii alternated rhythmically during the cell cycle. The activity of the "CO(2) concentrating mechanism" including the ability to accumulate CO(2) internally and the activity of carbonic anhydrase peaked after 6 to 9 hours of light and reached m...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.80.2.604

    authors: Marcus Y,Schuster G,Michaels A,Kaplan A

    更新日期:1986-02-01 00:00:00

  • Isolation and Characterization of the Genes Encoding Basic and Acidic Chitinase in Arabidopsis thaliana.

    abstract::Plants synthesize a number of antimicrobial proteins in response to pathogen invasion and environmental stresses. These proteins include two classes of chitinases that have either basic or acidic isoelectric points and that are capable of degrading fungal cell wall chitin. We have cloned and determined the nucleotide ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.3.907

    authors: Samac DA,Hironaka CM,Yallaly PE,Shah DM

    更新日期:1990-07-01 00:00:00